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PCR-based methodologies for detection and characterization of Listeria monocytogenes and Listeria ivanovii in foods and environmental sources

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Food Science and Human Wellness 2017年第2期6卷 39-59页

作者： Jin-Qiang Chen stephanie healey Patrick Regan Pongpan Laksanalamai Zonglin Hu Winchester Engineering&Analytical Center(WEAC) HFR-NE460Office of Regulatory AffairsUS Food and Drug Administration109 Holton StreetWinchesterMA 01890USA MD DHMH Laboratories Administration Division of MicrobiologyRoom 5281770 Ashland Ave.BaltimoreMD 21205USA

Listeria monocytogenes is an important foodborne pathogen responsible for listeriosis,a fatal *** is widely distributed in various foods and environmental *** this review,we focused on addressing PCR-based technologies,including conventional PCR,qPCR and droplet digital PCR(ddPCR).Specifically,we described(a)conventional PCR and mono-,duplex-and multiplex-qPCR methodologies;(b)development and applications of gene HlyA-,Iap-,PrfA–and SsrA-based conventional and qPCR assays as well as PCR assays targeting newly identified gene targets for specific detection of ***;differentiation of viable from dead *** by qPCR in conjugation with propidium monoazide pretreatment;PCR-based serotype identification of *** isolates;PCR-based detection of ***,infecting ruminants,differentiation of *** from other Listeria species;and sigB-gene based PCR identification of Listeria spp;(c)applications of ddPCR in detection of ***;and(d)application of qPCR assays in detection and subtyping of *** in milk and dairy products;meats,meat products and meat-processing environment;and seafood,seafood products and processing *** goal was to provide a relatively comprehensive overview of PCR-based methodologies available in detection,characterization and subtyping of various strains of *** in foods and environmental sources.

关键词： Foodborne pathogens Listeria monocytogenes Listeria ivanovii qPCR

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Prevalence and methodologies for detection,characterization and subtyping of Listeria monocytogenes and *** in foods and environmental sources

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Food Science and Human Wellness 2017年第3期6卷 97-120页

作者： Jin-Qiang Chena Patrick Regan Pongpan Laksanalamai stephanie healey Zonglin Hua Winchester Engineering Analytical Center(WEAC)HFR-NE460Office of Regulatory AffairsUS Food and Drug Administration109 Holton StreetWinchesterMA 01890USA MD DHMH Laboratories Administration Division of MicrobiologyRoom 5281770 Ashland Ave.BaltimoreMD 21205USA

Listeria monocytogenes,one of the most important foodborne pathogens,can cause listeriosis,a lethal disease for ***,which is closely related to ***,is also widely distributed in nature and infects mainly warm-blooded ruminants,causing economic ***,there are high priority needs for methodologies for rapid,specific,cost-effective and accurate detection,characterization and subtyping of *** and *** in foods and environmental *** this review,we(A)described *** and ***,world-wide incidence of listeriosis,and prevalence of various *** strains in food and environmental sources;(B)comprehensively reviewed different types of traditional and newly developed methodologies,including culture-based,antigen/antibody-based,LOOP-mediated isothermal amplification,matrix-assisted laser desorption ionization-time of flight-mass spectrometry,DNA microarray,and genomic sequencing for detection and characterization of *** in foods and environmental sources;(C)comprehensively summarized different subtyping methodologies,including pulsed-field gel electrophoresis,multi-locus sequence typing,ribotyping,and phage-typing,and whole genomic sequencing *** subtyping of *** strains from food and environmental sources;and(D)described the applications of these methodologies in detection and subtyping of *** in foods and food processing facilities.

关键词： Foodborne pathogens Listeria monocytogenes Listeria ivanovii Molecular characterization Subtyping

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维普期刊数据库

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EVER GREEN:A POST-OCCUPANCY BUILDING PERFORMANCE ANALYSIS OF LEED CERTIFIED HOMES IN NEW ENGLAND

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Journal of Green Building 2011年第4期6卷 138-145页

作者： Sandy J.Beauregard stephanie Berkland Simi Hoque Department of Environmental Conservation University of Massachusetts Amherst

The purpose of this research was to conduct a pilot study of LEED certified homes in New England one to five years after occupancy to determine whether they continue to perform at the level predicted during the certification *** criteria were defined to assess the performance of each home:(1)energy consumption for heating,cooling,lighting,electronics and appliances;(2)building envelope and air infiltration;(3)operations and maintenance;and(4)occupant satisfaction and indoor air *** qualitative and quantitative methods including blower door and duct blaster tests,utility bill analysis,and an occupant satisfaction survey were used to measure the post-occupancy performance of seven LEED certified homes in New *** were found between the original building performance as determined at the time of certification and the current level of building *** this is limited,initial research,these findings indicate a need for further post-occupancy research that can provide feedback to green certification programs,allowing them to be modified as necessary to better reflect the actual environmental impacts of certified buildings.

关键词： post-occupancy LEED residential certified homes home performance energy consumption

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