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PEP7 acts as a peptide ligand for the receptor kinase SIRK1 to regulate aquaporin-mediated water influx and lateral root growth

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Molecular Plant 2022年第10期15卷 1615-1631页

作者： Jiahui Wang Lin Xi Xu Na Wu stefanie konig Leander Rohr Theresia Neumann Jan Weber Klaus Harter Waltraud X.Schulze Department of Plant Systems Biology University of Hohenheim70593 StuttgartGermany School of Life Science Center for Life SciencesYunnan University650091 KunmingPeople's Republic of China Center for Plant Molecular Biology University of Tubingen72076 TubingenGermany

Plant receptors constitute a large protein family that regulates various aspects of development and responses to external *** characterization of this protein family and the identification of their ligands remain major challenges in plant ***,we identified plasma membrane-intrinsic sucrose-induced receptor kinase 1(SIRK1)and Qian Shou kinase 1(QSK1)as receptor/co-receptor pair involved in the regulation of aquaporins in response to osmotic conditions induced by *** this study,we identified a member of the elicitor peptide(PEP)family,namely PEP7,as the specific ligand of th receptor kinase ***7 binds to the extracellular domain of SIRK1 with a binding constant of 1.44±0.79μM and is secreted to the apoplasm specifically in response to sucrose *** of a signaling complex involving SIRK1,QSK1,and aquaporins as substrates is mediated by alterations in the external sucrose concentration or by PEP7 ***,the presence of PEP7 induces the phosphorylation of aquaporins in vivo and enhances water influx into *** water influx,in turn,led to delayed lateral root development in the pep7 *** loss-of-function mutant of SIRK1 is not responsive to external PEP7 treatment regarding kinase activity,aquaporin phosphorylation,water influx activity,and lateral root *** together,our data indicate that the PEP7/SIRK1/QSK1 complex represents a crucial perception and response module that mediates sucrose-controlled water flux in plants and lateral root development.

关键词： receptor kinase peptide signaling receptor-ligand pair sugar signaling regulation of water influx

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Transcriptional Activation and Production of Tryptophan-Derived Secondary Metabolites in Arabidopsis Roots Contributes to the Defense against the Fungal Vascular Pathogen Verticillium Iongisporum

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Molecular Plant 2012年第6期5卷 1389-1402页

作者： Tim Iven stefanie konig Seema Singh Susanna A. Braus-Stromeyer Matthias Bischoff Lutz F. Tietze Gerhard H. Braus Volker Lipka Ivo Feussner Wolfgang Droge-Laser Julius-Maximilians-Universitat Wurzburg Julius-von-Sachs-lnstitut Pharmazeutische Biologie Julius-von-Sachs-Platz 2 97082 Wurzburg Germany Georg-August-Universitat Albrecht-von-Haller-lnstitut fur Pflanzenwissenschaften Biochemie der Pflanze Justus-von-Liebig-Weg 11 37077 GottingenGermany Georg-August-Universitat Institut for Mikrobiologie und Genetik Molekulare Mikrobiologie und Genetik Grisebachstr. 8 37077 Gottingen Germany Georg-August-Universitat Institut for Organische und Biomolekulare Chemie AG Tietze Tammannstrasse 2 37077 Gottingen Germany Georg-August-Universitat Schwann-Schleiden-Forschungszentrum for Molekulare Zellbiologie Abteilung Zellbiologie der Pflanze Julia-Lermontowa-Weg3 37077 Gottingen Germany

The soil-borne fungal pathogen Verticillium Iongisporum causes vascular disease on Brassicaceae host plants such as oilseed rape. The fungus colonizes the root xylem and moves upwards to the foliage where disease symptoms become visible. Using Arabidopsis as a model for early gene induction, we performed root transcriptome analyses in re- sponse to hyphal growth immediately after spore germination and during penetration of the root cortex, respectively. Infected roots showed a rapid reprogramming of gene expression such as activation of transcription factors, stress-, and defense-related genes. Here, we focused on the highly coordinated gene induction resulting in the production of tryp- tophan-derived secondary metabolites. Previous studies in leaves showed that enzymes encoded by CYP81F2 and PEN2 （PENETRATION2） execute the formation of antifungal indole glucosinolate （IGS） metabolites. In Verticillium-infected roots, we found transcriptional activation of CYP81F2 and the PEN2 homolog PEL 1 （PEN2-LIKE1）, but no increase in antifungal IGS breakdown products. In contrast, indole-3-carboxylic acid （13CA） and the phytoalexin camalexin accumulated in infected roots but only camalexin inhibited Verticillium growth in vitro. Whereas genetic disruption of the individual metabolic pathways leading to either camalexin or CYP81F2-dependent IGS metabolites did not alter Verticillium-induced disease symptoms, a cyp79b2 cyp79b3 mutant impaired in both branches resulted in significantly enhanced susceptibility. Hence, our data provide an insight into root-specific early defenses and suggest tryptophan-derived metabolites as active anti- fungal compounds against a vascular pathogen.

关键词： Verticillium Iongisporum Arabidopsis root transcriptome camalexin indole glucosinolates.

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