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Optical prediction of single muscle fiber force production using a combined biomechatronics and second harmonic generation imaging approach

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Light(Science & Applications) 2018年第1期7卷 289-302页

作者： Dominik schneidereit stefanie Nübler Gerhard Prölß Barbara Reischl Sebastian schürmann Oliver J Müller Oliver Friedrich Institute of Medical Biotechnology Friedrich-Alexander-University(FAU)Erlangen-NürnbergPaul-Gordan-Str.391052 ErlangenGermany Erlangen Graduate school in Advanced Optical Technologies(SAOT) FAU Erlangen-NürnbergPaul-Gordan-Str.791052 ErlangenGermany Muscle Research Center Erlangen(MURCE) Friedrich-Alexander-University Erlangen-NürnbergErlangenGermany Department of Internal Medicine III University of KielArnold-Heller-Str.324105 KielGermany DZHK(German Center for Cardiovascular Research)Partner Site Hamburg/Kiel/Lübeck KielGermany

Skeletal muscle is an archetypal organ whose structure is tuned to match *** magnitude of order in muscle fibers and myofibrils containing motor protein polymers determines the directed force output of the summed force vectors and,therefore,the muscle’s power performance on the structural *** and function can change dramatically during disease states involving chronic *** remodeling of the cytoarchitecture has been pursued using noninvasive and label-free multiphoton second harmonic generation(SHG)***,structure parameters can be extracted as a measure of myofibrillar order and thus are suggestive of the force output that a remodeled structure can still ***,to date,the parameters have only been an indirect measure,and a precise calibration of optical SHG assessment for an exerted force has been elusive as no technology in existence correlates these *** engineered a novel,automated,high-precision biomechatronics system into a multiphoton microscope allows simultaneous isometric Ca2+-graded force or passive viscoelasticity measurements and SHG *** this MechaMorph system,we studied force and SHG in single EDL muscle fibers from wt and mdx mice;the latter serves as a model for compromised force and abnormal myofibrillar *** present Ca2+-graded isometric force,pCa-force curves,passive viscoelastic parameters and 3D structure in the same fiber for the first ***,we provide a direct calibration of isometric force to morphology,which allows noninvasive prediction of the force output of single fibers from only multiphoton images,suggesting a potential application in the diagnosis of myopathies.

关键词： structure harmonic fibers

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在循环作业周期内灵活应用的机器人

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现代制造 2017年第5期 18-19页

作者： stefanie schnberger

很多行业的生产过程中都涉及对部件进行标记以及识别,为了确保生产流程的持续性,打印标签作业必须可靠进行,而作业灵活且高效的机器人臂恰好能够满足这一要求。位于德国巴登符滕堡州的Topex公司成立于1984年,如今约有员工50人,该公司除...

很多行业的生产过程中都涉及对部件进行标记以及识别,为了确保生产流程的持续性,打印标签作业必须可靠进行,而作业灵活且高效的机器人臂恰好能够满足这一要求。位于德国巴登符滕堡州的Topex公司成立于1984年,如今约有员工50人,该公司除了设在Erkenbrechtsweiler的公司本部外,在德国北威州以及中国和美国还设有分公司。Topex公司是一家“标签标记打印”领域的专用机器生产厂家,其研发的设备

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Protective effects of ischemic preconditioning and application of lipoic acid prior to 90 min of hepatic ischemia in a rat model

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World Journal of Gastroenterology 2007年第27期13卷 3692-3698页

作者： Friedrich Duenschede Kirsten Erbes Nina Riegler Patrick Ewald Achim Kircher stefanie Westermann Arno schad Imke Miesmer Simon Albrecht-schck Ines Gockel Alexandra K Kiemer Theodor Junginger Department of General and Abdominal Surgery University hospital Mainz Langenbeckstr.1 D-55131 Mainz Germany Institute for Pathology University hospital Mainz Germany Department of Pharmacy Pharmaceutical Biology Saarland University 66123 Saarbrücken Germany

AIM： To compare different preconditioning strategies to protect the liver from ischemia/reperfusion injury focusing on the expression of pro- and anti-apoptotic proteins. Interventions comprised different modes of ischemic preconditioning （IP） as well as pharmacologic pretreatment by α-lipoic acid （LA）. METHODS： Several groups of rats were compared： sham operated animals, non-pretreated animals （nt）, animals receiving IP （10 rain of ischemia by clamping of the portal triad and 10 min of reperfusion） prior to sustained ischemia, animals receiving selective ischemic preconditioning （IPsel, 10 min of ischemia by selective clamping of the ischemic lobe and 10 rain of reperfusion） prior to sustained ichemia, and animals receiving 500 1μmol α-LA injected i.v. 15 min prior to the induction of 90 min of selective ischemia. RESULTS： Cellular damage was decreased only in the LA group. TUNEL-positive hepatocytes as well as necrotic hepatocyte injury were also decreased only by LA（19 ± 2 vs 10 ± 1, P〈 0.05 and 29 ± 5 vs 12 ± 1, P 〈 0.05）. Whereas caspase 3- activities in liver tissue were unchanged, caspase 9- activity in liver tissue was decreased only by LA pretreatment （3.1 ± 0.3 vs 1.8 ± 0.2, P 〈 0.05）. Survival rate as the endpoint of liver function was increased after IP and LA pretreatment but not after IPsel. Levels of lipid peroxidation （LPO） in liver tissue were decreased in the IP as well as in the LA group compared to the nt group. Determination of pro- and anti-apoptotic proteins showed a shift towards anti-apoptotic proteins by LA. In contrast, both our IP strategies failed to influence apototic cell death. CONCLUSION： IP, consisting of 10 min of ischemia and 10 min of reperfusion, ischemia/reperfusion injury protects only partly against of the liver prior to 90 min of selective ischemia. IPsel did not influence ischemic tolerance of the liver. LA improved tolerance to ischemia, possibly by downregulation of pro-apoptotic Bax.

关键词： Warm liver ischemia Liver preconditioning Apoptosis Lipid peroxidation Pharmacological preconditioning

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