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Point-of-need molecular processing of biosamples using portable instrumentation to reduce turnaround time

Biosafety and Health 2020年第3期2卷 177-182页

作者： Manousos E.Kambouris stavroula siamoglou Zoe Kordou Aphroditi Milioni Stamatis Vassilakis stavroula Goudoudaki stavroula Kritikou Yiannis Manoussopoulos Aristea Velegraki George P.Patrinos Department of Pharmacy University of PatrasRio Patras 26504Greece Department of Microbiology School of MedicineNational and Kapodistrian University of AthensMikras Asias 75Athens 11527Greece Plant Protection Division of Patras Institute of Industrial and Forage PlantsN.E.O&AmerikisPatras 26004Greece United Arab Emirates University College of Medicine and Health SciencesDepartment of PathologyAl-AinUnited Arab Emirates United Arab Emirates University Zayed Center of Health SciencesAl-AinUnited Arab Emirates

Infectious agents,both standard and emerging or engineered,might initiate outbreaks with global impact and/or existential-level threat *** agents might infect human,livestock,exploitable plant cultures or the environment at *** of responding by sampling teams which may also carry out prolonged surveillance,a more effective concept is to pre-process the samples,possibly to nucleic acid extract so as to transport them without biological risks by standard channels,or to process them up to a point and return the results,leaving the samples which only need specialized tests and dedicated *** this context a portable device,allowing multiple routine and crisis-management applications due to its inherent flexibility,is scrutinized against alternatives and possible needs so as to develop a novel standard of surveillance and intervention,meant to facilitate containment by providing faster much more reliable laboratory results at a fraction of older generation mobile nucleic acids *** steps,such as agarose in pills and buffers pre-packaged in expendable syringes with pistons locked by improvised safety features may improve the Complexity Level of the whole system so as to make it appealing to intervention/crisis management personnel,while the instrument proper may double as backup for respective benchtop devices.

关键词： Point-of-Need Biosurveillance Public health Biosecurity-biosafety Portable PCR Portable NGS Quarantine pathogens

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