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Research Progress of Immunosensor Assay for Several Phytohormones

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Research Progress of Immunosensor Assay for Several Phytohor...

2005年全国植物生长物质研讨会

作者： Langtao Xiao, Ruozhong Wang, Jin Li, Guoli Shen hunan Provincial key laboratory of Phytohormones, hunan Agricultural university. Changsha 410128 state key laboratory of chemo/biosensing and chemometrics, hunan university Changsha 410082

＜正＞Phytohormones play very important roles at almost all developmental stages of plants. Because of their extremely low concentrations in plant tissues and they are highly sensitive to environmental factors such as light, heat and oxygen, sensitive assay is a limiting factor for phytohormonal research. All traditional phytohormonal assays including bioassay, GC, HPLC, ELISA and RIA have their disadvantages. Mainly based on radioimmunoassay and biosensor technology, immunosensor assay is a new technology for phytohormonal analysis proposed by hunan Provincial key laboratory of Phytohormones and Growth Development and state key laboratory of chemo/biosensing and chemometrics at hunan university in ***

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Amperometric Immunosensor for Abscisic Acid Based on in Situ Chemical Reductive Growth of Gold Nanoparticles on Glassy Carbon Electrode

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Amperometric Immunosensor for Abscisic Acid Based on in Situ...

2007中国植物生理学会全国学术会议

作者： Ruozhong WANG~1 Langtao XIAO~(1*) Minghui YANG~2 Junhui DING~1 Yuwei LI~1 Guoli SHEN~2 (1 hunan Provincial key laboratory of Phytohormones and Growth Development,hunan Agricultural university,Changsha 410028) (2 Chemistry and Chemical Engineering College,state key laboratory of chemo/biosensing and chemometrics.hunan university,Changsha 410082)

＜正＞Gold nanoparticles（AuNPs）attached to glassy carbon（GC）electrode surface was successfully prepared by using a simple in situ chemical reduction *** this method,1～10 nm gold layer was first sputtered uniformly onto the electrode surface,AuNPs could be grown directly on the gold layer with immersion the electrode into the grown solution containing H2AuC14 and ascorbic *** gold layer sputtered uniformly serves as“seed”for the following growth of *** formed AuNPs were used for adsorption of antibody for the detection of abscisic acid（ABA）.The determination is based on an enzyme-linked competitive immunoreaction between free ABA and ABA labeled with HRP to bind on the anti-ABA IgG immobilized on the electrode *** linear response of the sensor is from 100 ng/ml to 100?g/ml with a detection limit of 50 ng/mL.

关键词： Gold nanoparticles(AuNPs) Abscisic acid(ABA) Immunoreaction

A new member of GHMP kinase family required for abscisic acid signaling in Arabidopsis

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A new member of GHMP kinase family required for abscisic aci...

从植物科学到农业发展——2012全国植物生物学大会

作者： Hongping Chang~§,Congying Yuan~§,Dashi Yu,Yu Wang,Shuai Hu,Xuanming Liu, Xiushan Li,Xinhong Guo~(*§) School of Biology,state key laboratory of chemo/biosensing and chemometrics,hunan university, Changsha 410082 P.R.China

Numerous studies have indicated that phosphorylation is an important component of ABA *** GHMP kinase family is a family of kinase *** of this family include the galactokinase,homoserine kinase,mevalonate kinase,and phosphomevalonate *** main roles are to catalyze protein phosphorylation by transfering the phosphate of ATP to amino acid residues of the specific substrate,which were involved in a variety of signal ***,we showed that a new member of GHMP kinase family(GHMP1) controlled responses to ABA in seed germination,dormancy,and seedling growth in Arabidopsis *** results showed that two deletion mutants were less sensitive to exogenous ABA and NaCl in seed germination and root growth inhibition compared with *** real time PCR results showed that there were a high transcription level in roots and flowers,but low level in leaf,petiole,and *** expression of GHMP1 gene was induced by ABA and *** transcription levels of a series of stress response genes(abi1,abi2,KIN1/2,SOS2/3,OST1,RD29A/B,RAB18, RD22,DREB1/2A) related to ABA and salt responses were compared by real-time PCR between the two ghmp mutants and wild *** distinct differences in transcription levels in these genes were displayed because of the deletion of GHMP 1 *** work provided some invaluable references for the physiological functions of the other GHMP gene family members.

关键词： Arabidopsis ABA GHMP1 Physiological function

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电化学DNA传感器的研制及其医学应用

分析测试学报 2005年第1期24卷 123-128页

作者：邹小勇陈汇勇李荫中山大学化学与化学工程学院湖南大学化学生物传感与计量学国家重点实验室湖南长沙410082

对近几年发展起来的电化学DNA传感器的研究工作进行了讨论 ,详细论述了该类传感器的工作原理、探针固定化技术、杂交指示剂的类型以及在基因诊断、药物分析等方面的医学应用 ,并展望了应用前景和发展方向。

关键词：电化学DNA传感器杂交指示剂修饰电极基凶诊断

AtWNK9 regulates root elongation and is involved in ABA response in Arabidopsis

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AtWNK9 regulates root elongation and is involved in ABA resp...

从植物科学到农业发展——2012全国植物生物学大会

作者： Minmin Xie~1,Reqing He~1,Guifang Duan~1,Qiuhong Duan~1,Jianzhong Lin~1,Dongying Tang~1,Xiaoying Zhao~(1*),Xuanming Liu~(1,2) 1 Bioenergy and Biomaterial Research Center,College of Biology,hunan university,Changsha 410082. 2 state key laboratory of chemo/biosensing and chemometrics,hunan university,Changsha 410082.

AtWNK9(with no lysine kinase) protein kinase gene is root-specific expressed in Arabidopsis,but its function remains *** we report that wnk9 mutant showed shorter primary root,while the over-expression lines of WNK9 had longer primary root compared with wild type *** mRNA expression of WNK9 was induced by ABA. When grown in the presence of ABA the seedlings exhibited ABA-inhibited root elongation,and the WNK9 over-expression lines showed hypersensitivity to ABA,however wnk9 mutant showed lower sensitivity to ABA compared with wild type seedlings. Moreover,the WNK9 over-expression lines exhibited decreased stomatal open,lower water-loss rate,more proline accumulation compared with wild type in response to ABA, while reverse phenotype was showed in the wnk9 mutant.Q-PCR results showed that a series of ABA-responsive genes were induced by ABA,and their expression level was lower in the wnk9 mutant but higher in the over-expression lines than that in the wild type. Subcellular localization result showed that WNK9 localizes in cell *** results indicate that WNK9 might regulate root elongation through ABA signal transduction.

关键词： AtWNK9 Root elongation ABA Arabidopsis

Exogenous gibberellin affects reserve accumulation during seeds filling in Brassica Napus

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Exogenous gibberellin affects reserve accumulation during se...

从植物科学到农业发展——2012全国植物生物学大会

作者： Xingqun Huang~1,Xiaoying Zhao~1,Reqing He~1,Bo Zhou~1,Wusheng Peng~3,Yuhong Zhuo~3, Jianzhong Lin~1,Dongying Tang~1,Jihong Zhang~1,Xuanming Liu~(*1,2) 1 Bioenergy and Biomaterial Research Center,College of Biology,hunan university,Changsha 410082. 2 state key laboratory of chemo/biosensing and chemometrics,hunan university,Changsha 410082. 3 Academy of Seed Industry of hunan Yahua,Changsha 410001.

Exogenous Gibberellins(GAs) are applied in agriculture broadly and their physiological functions and biochemistry mechanism become more and more clear,but little is known about the effect of GAs on the reserve substance accumulation during seed *** this paper,it is shown that GAs acts as a physiological regulator in the regulation of carbohydrate,protein,and lipids accumulation in developing seeds of Brassica *** was found that the oil and total sugar content of seeds increased by 4.70%and 6.77%,and the crude protein content decreased by 3.18%in the samples treated with GA at 15 DAF (days after flower);the total sugar content increased by 4.10%,and the crude protein content decreased by 5.77%treated with GA3 at 25 DAF;the crude protein and total sugar content increased by 5.85%and 5.04%treated with GA3 at 35DAF.Q-PCR analysis results showed the transcription levels of three transcription factors(LEC1,LEC2 and WRI), sucrose transporter SUC2,and key oil-synthesis gene DGAT were upregulated in response to GA at 15DAF,and the transcription factor ABB,ABI5,and storage protein gene Napin were downregulated in GA-treated samples at 25DAF,while upregulated at 35DAF,which consistent with the reserve accumulation *** results indicated that the effect of GAs on the reserve accumulation may be due to the altered lipid,protein,sugar metabolism related gene expression.

关键词： Gibberellins reserve accumulation gene expression Seed Maturing Brassica napuss

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基于双层类脂膜的去甲肾上腺素传感器

分析测试学报 2005年第1期24卷 53-55,59页

作者：李荫谭学才梁汝萍蔡沛祥邹小勇中山大学化学与化学工程学院广西民族学院化工系广西南宁530006 湖南大学化学生物传感与计量学国家重点实验室湖南长沙410082

报道了去甲肾上腺素 (NE)在月桂酸 -双层类脂膜修饰的玻碳电极上的电化学行为 ,在 +0.6～ -0.2V(***/AgCl)电位范围内 ,于pH7.0的0.01mol/L的KH2PO4-Na2HPO4 底液中 ,去甲肾上腺素产生很灵敏的氧化峰电流。氧化峰电流与去甲肾上腺素浓度... 详细信息

报道了去甲肾上腺素 (NE)在月桂酸 -双层类脂膜修饰的玻碳电极上的电化学行为 ,在 +0.6～ -0.2V(***/AgCl)电位范围内 ,于pH7.0的0.01mol/L的KH2PO4-Na2HPO4 底液中 ,去甲肾上腺素产生很灵敏的氧化峰电流。氧化峰电流与去甲肾上腺素浓度在1.7×10 -5～5.9×10 -4mol/L范围内呈良好线性关系。

关键词：去甲肾上腺素双层类脂膜修饰电极静电吸附作用

Inductance-based sensing technique for wireless,remote-query measurement in liquid media

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Science China Chemistry 2010年第6期53卷 1391-1397,1460页

作者： MA Lin,NIE Zhou,HUANG Yan & YAO ShouZhou state key laboratory of chemo/biosensing and chemometrics,College of Chemistry and Chemical Engineering,hunan university,Changsha 410082,China 1. state key laboratory of chemo/biosensing and chemometrics College of Chemistry and Chemical Engineering Hunan University Changsha 410082 China

A novel inductance-based sensing technique is presented for remote query measurement in different liquid media including organic solvents and inorganic *** inorganic solutions tested included salt solutions of different concentrations,and the organic solvents detected included 1,4-dioxane and *** extend the application of the sensor,bacterial culture media were also detected,and the growth of Escherichia coli (***) was *** influential factors which may affect the inductance responses were studied in *** was found that quantitative relationships exist between the sensor’s inductance response and the physico-chemical parameters of the liquid *** sensor’s inductance response (L) decreases with the increase of salt concentration (C) and its ionic valence (e) according to a semi-logarithmic equation LgL =-aeC + b,where a and b are constants,which is in accordance with the theoretically deduced *** inductance variation rate (ΔK) increases directly with the temperature (T): ΔK = a′ T + b′.As for organic solutions,the sensor’s inductance was found to increase with the increasing permittivity of the organic *** wireless sensor we designed is simple and easy to *** has the potential for remote determination of not only chemical substances but also microbiological species such as *** the newly developed inductance-based sensor,the pathogenic *** was monitored with a limit of detection of 10 cells/mL and a linear semi-logarithmic range of 1.0 × 101 to 2.5 × 109 cells/mL.

关键词： inductance sensor remote-query liquid medium wireless bacterial detection E. coli.

Eu^(3+)-doped LaPO_4 and LaAlO_3 nanosystems and their luminescence properties

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Science China Chemistry 2009年第8期52卷 1104-1112页

作者： WAN Qiang,HE YuPing,DAI Ning & ZOU BingSuo state key laboratory of state key laboratory of chemo/biosensing and chemometrics and key laboratory for Micro-Nano Opto-Electronic Devices of Ministry of Education,hunan university,Changsha 410082,China 1. state key laboratory of state key laboratory of chemo/biosensing and chemometrics and key laboratory for Micro-Nano Opto-Electronic Devices of Ministry of Education Hunan University Changsha 410082 China

Eu3+ ion-doped LaPO4 nanowires or nanorods have been successfully synthesized by a simple hydrothermal *** influence of varying the hydrothermal and subsequent sintering conditions on the morphology and structure of the LaPO4 host has been investigated by scanning electron microscopy(SEM) and X-ray diffraction(XRD).For comparison,the Eu3+ ions were also doped into monoclinic monazite LaPO4 nanoparticles and perovskite LaAlO3 *** relative intensities of the emission lines of the LaPO4:Eu3+ nanosystems were essentially independent of their *** optimal doping concentrations in the monoclinic LaPO4 and perovskite LaAlO3 nanosystems were determined to be about 5.0 and 3.5 mol%,*** appropriate UV-radiation,the red light emitted from LaAlO3:Eu3+(3.5 mol%) was brighter than that from LaPO4:Eu3+(5.0 mol%) nanomaterial,resulting from differences in their spin-orbit couplings and covalence,which indicates that the nanoscale LaAlO3 is a promising host material for rare earth ions.

关键词： doping synthesis luminescence nanosystem