检索结果-南通市图书馆

中国兽医科学 2016年第1期46卷 116-121页

作者：王秋霞郭利亚陈蕾窦永喜欧长波余燕张艳红马金友刘兴友才学鹏河南科技学院动物科学学院河南新乡453003 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室甘肃兰州730046 甘肃农业大学动物科学技术学院甘肃兰州730070 中国农业科学院北京畜牧兽医研究所动物营养国家重点实验室北京100193

采用巢式PCR方法获得未见报道的绵羊Nectin4基因。根据GenBank上登录的牛的Nectin4mRNA序列信息设计并合成10对引物;从绵羊肺支气管组织中提取总RNA,采用随机引物和Oligo(dT)将其反转录为cDNA;利用巢式PCR原理和PCR引物错配导致非特异... 详细信息

采用巢式PCR方法获得未见报道的绵羊Nectin4基因。根据GenBank上登录的牛的Nectin4mRNA序列信息设计并合成10对引物;从绵羊肺支气管组织中提取总RNA,采用随机引物和Oligo(dT)将其反转录为cDNA;利用巢式PCR原理和PCR引物错配导致非特异反应的特点,改变常规PCR反应条件,将10对引物分为2组,通过两轮PCR即获得大小片段相符的片段;TA克隆后测序证实已成功获得绵羊Nectin4基因的完整ORF,证明本方法可作为一种获取未知基因的方法,较RACE等其他传统技术有明显优势;构建了重组表达质粒pCMV-Myc-Nectin 4,在CHO细胞的细胞质中获得高效表达,经Western-blot分析,表达的Nectin 4蛋白的大小与预期符合。绵羊Nectin4基因的成功扩增为后续对其在病毒入侵中的功能进行研究奠定了基础。

关键词： Nectin4 巢氏PCR 载体构建蛋白质表达

Dose-dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome(Schistosomajaponicum)by RNA Interference

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2005年第6期37卷 386-390页

作者： Guo-Feng CHENG~1 Jiao-Jiao LIN~1 Yi SHI~2 You-Xin JIN~2 Zhi-Qiang FU~1 Ya-Mei JIN~1 Yuan-Cong ZHOU~2 You-Min CA~1 1 Shanghai institute of animal Parasitology, chinese academy of agricultural sciences, key Lahoratory of animal Parasitology,Ministry of Agriculture, Shanghai 200232, China 2 state key laboratory, of Molecular Biology institute of Biochemistry and Cell Biology Shanghai institutes for Biological sciences, chinese academy of sciences. Shanghai 200031, China Shanghai institute of animal Parasitology Chinese Academy of Agricultural Sciences Key Laboratory of Animal Parasitology Ministry of Agriculture Shanghai 200232 China.

The gynecophoral canal protein gene SjGCP of Schistosoma japonicum that is necessary forthe pairing between the male and female worms is specifically expressed in the adult male *** is widely distributed in the adult female worm after *** transcription-polymerase chainreaction(RT-PCR)and immunofluorescence were employed to analyze the relationship between the RNAieffect and dsRNA dosage in the *** results revealed that the inhibition of SjGCP expression bysiRNA is ***-PCR analysis showed that the SjGCP transcript level was reduced by 75%when 100 nM dsRNA was applied.

关键词： RNA interference gynecophoral canal protein gene Schistosoma japonicum dosage

The Effect of Bovine IFN-α on the Immune Response in Guinea Pigs Vaccinated with DNA Vaccine of Foot-and-Mouth Disease Virus

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第10期36卷 701-706页

作者： Hui-Chen GUO, Zai-Xin LIU*, Shi-Qi SUN, Qing-Wen LENG1, Dong LI, Xiang-Tao LIU, and Qing-Ge XIE* key laboratory of animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research institute, chinese academy of agricultural sciences, Lanzhou 730046, China College of animal Science and Technology, Shihezi University, Shihezi 832003, China 1 key laboratory of animal Virology of Ministry of Agriculture Lanzhou Veterinary Research Institute Chinese Academy of Agricultural SciencesLanzhou 730046 China College of animal Science and Technology Shihezi UniversityShihezi 832003 China

In this study, we constructed recombinant plasmid pcDNA3.1/P12X3C3D including P1, 2A, 3C, 3D and part of 2B gene of FMDV and pcDNA3.1/IFN containing the gene encoding bovine IFN-α. We inoculated the DNA vaccine pcDNA3.1/P12X3C3D with or without pcDNA3.1/IFN to evaluate the effi- ciency of this DNA vaccine and the immunogenicity of DNA vaccine enhanced by the co-delivery with pcDNA3.1/IFN. After two times of vaccination with DNA vaccine, all of guinea pigs were challenged with 103 ID50 FMDV type O. Anti-FMDV antibody levels were detected by ELISA and T lymphocyte proliferation response was tested by MTT assay. The result shows that guinea pigs inoculated by pcDNA3.1/P12X3C3D alone or with pcDNA3.1/IFN generated specific antibodies and induced an FMDV-specific T lymphocyte proliferation response. FMDV challenge tests showed that one in four guinea pigs immunized by pcDNA3.1/ P12X3C3D with pcDNA3.1/IFN was protected from the FMDV serotype O infection. This result indicated that the efficiency of the DNA vaccine was enhanced by co-delivery with pcDNA3.1/IFN. However, the protection rate was considerably lower than that immunized with conventional FMD vaccine.

关键词： foot-and-mouth disease virus (FMDV) DNA vaccine IFN-α gene vaccination

Diversity and evolution of MicroRNA gene clusters

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China(Life sciences) 2009年第3期52卷 261-266页

作者： ZHANG YanFeng,ZHANG Rui & SU Bing state key laboratory of Genetic Resources and Evolution,Kunming institute of Zoology,chinese academy of sciences,Kunming 650223,China state key laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming China

microRNA(miRNA) gene clusters are a group of miRNA genes clustered within a proximal distance on a *** a large number of miRNA clusters have been uncovered in animal and plant genomes,the functional consequences of this arrangement are still poorly *** in a polycistron,the coexpressed miRNA clusters are pivotal in coordinately regulating multiple processes,including embryonic development,cell cycles and cell *** this review,based on recent progress,we discuss the genomic diversity of miRNA gene clusters,the coordination of expression and function of the clustered miRNAs,and the evolutionarily adaptive processes with gain and loss of the clustering miRNA genes mediated by duplication and transposition events.

关键词： microRNA gene clusters gene duplication adaptive evolution transposition

MicroRNA in cell differentiation and development

在线全文

学校读者我要写书评

暂无评论

Science China(Life sciences) 2009年第3期52卷 205-211页

作者： SHI Yi & JIN YouXin state key laboratory of Molecular Biology,Shanghai institute of Biochemistry and Cell Biology,Shanghai institutes for Biological sciences,chinese academy of sciences,Shanghai 200031,China state key laboratory of Molecular Biology Shanghai Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

The regulation of gene expression by microRNAs(miRNAs) is a recently discovered pattern of gene regulation in animals and *** have been implicated in various aspects of animal development and cell differentiation,such as early embryonic development,neuronal development,muscle development,and lymphocyte development,by the analysis of genetic deletions of individual miRNAs in *** studies show that miRNAs are key regulators in animal development and are potential causes of human *** we review some recent discoveries about the functions of miRNAs in cell differentiation and development.

关键词： miRNA embryonic development neuronal development muscle development lymphocyte development

animal reproduction and physiology: from basis to application

在线全文

学校读者我要写书评

暂无评论

Science China(Life sciences) 2010年第4期53卷 399-400页

作者： GUI JianFang,Prof. state key laboratory of Freshwater Ecology and Biotechnology, institute of Hydrobiology, chinese academy of sciences, Wuhan 430072, China state key laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan China

animal reproduction and physiology is one of the traditional subjects in biology, and also one of the most rapidly developing fields because it is related to human

关键词： from basis to application animal reproduction and physiology

STUDY OF DIGITAL MANAGEMENT SYSTEM OF EPIDEMIC PREVENTION ON INDUSTRIALIZED BREEDING PIG FARM

在线全文

学校读者我要写书评

暂无评论

STUDY OF DIGITAL MANAGEMENT SYSTEM OF EPIDEMIC PREVENTION ON...

The associations between the environmental exposure to polychlorinated biphenyls (PCBs) and breast cancer risk and progression

第四届智能化农业信息技术国际学术会议

作者： Qingyao Luo Benhai Xiong Jianqiang Lu Zhihong Pang institute of animal sciences chinese academy of agricultural sciences state key laboratory of animal nutrition Beijing 100094 China

＜正＞Based on Visual Basic 6.0,Access relationship databases technologies,this study analyzes some metadata and designs structured datasheets for some main production processes of a breeding farm as a Industrialized running such as immunity,quarantine, prevention,sickening,diagnosis and curing and some production elements as bacterins and medicine et al.,and developes a set of digital management information systems for epidemic prevention by combining pre-defining pig herd special immunity programs parameters based on different types of pigs and different kinds of *** results show that it supplies convenient original data recording windows with intelligence,and it has beforehand hint about when and which pigs will be done what kind of immunity,and it can inspect health state of the whole herd dynamically by calculating some key parameters as incidence of a disease,recovered rate, cull of diseases,and mortality of *** the same time the system also supplies chart analysis on some statistics ***,it points out that a future orientation of this kind of system is to develop a B/S structure model from its C/S system.

关键词： breeding pig farm epidemic prevention digital management intelligent prompt

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Science China Chemistry 2010年第5期53卷 974-979页

作者： LIU SiJin ,ZHANG ShuPing,QU Chen,LIU Wei & DU YuGuo state key laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental sciences,chinese academy of sciences,Beijing 100085,China state key laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China

Polychlorinated biphenyls(PCBs) are chlorinated biphenyl compounds with wide applications in the *** spite of a ban on their production in the late 1970s,PCBs,as a group of POPs,are still persistent and widely spread in the environment,posing potential threats to human *** role of PCBs as etiologic agents for breast cancer has been intensively explored in a variety of in vivo,animal and epidemiologic *** investigations indicated higher levels of PCBs in mammary tissues or sera corresponded to the occurrence of breast cancer,but later studies showed no positive association between PCB exposure and breast cancer *** recent data suggested that the CYP1A1 m2 polymorphisms might add increased risk to the etiology of breast cancer in women with environmental exposure to *** are implicated in advancing breast cancer progression,and our unpublished data reveals that PCBs activate the ROCK signaling to enhance breast cancer ***,the correlation between PCB exposure and breast cancer risk warrants further careful investigations.

关键词： Polychlorinated biphenyls (PCBs) environmental exposure CYP1A1 polymorphisms breast cancer risk tumor progression

The role of MC1R gene in buffalo coat color

在线全文

学校读者我要写书评

暂无评论

Science China(Life sciences) 2010年第2期53卷 267-272页

作者： MIAO YongWang1,2,WU GuiSheng3,WANG Lei2,LI DaLin4,TANG ShouKun5,LIANG JianPing2,MAO HuaMing2,LUO HuaiRong3 & ZHANG YaPing1,6 1 laboratory for Conservation and Utilization of Bio-resource,Yunnan University,Kunming 650091,China 2 Faculty of animal Science and Technology,Yunnan agricultural University,Kunming 650201,China 3 Kunming institute of Botany,chinese academy of sciences,Kunming 650204,China 4 Yunnan institute of Buffalo Science and Technology,Kunming 650021,China 5 animal Husbandry and Veterinary Station of Luxi city,Luxi 678400,China 6 state key laboratory of Genetic Resources and Evolution,Kunming institute of Zoology,chinese academy of sciences,Kunming 650223,China laboratory for Conservation and Utilization of Bio-resource Yunnan University Kunming China Faculty of animal Science and Technology Yunnan Agricultural University Kunming China Kunming institute of Botany Chinese Academy of Sciences Kunming China Yunnan institute of Buffalo Science and Technology Kunming China animal Husbandry and Veterinary Station of Luxi city Luxi China state key laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming China

Melanocortin-1 receptor (MC1R) plays a major role in pigmentation in many *** investigate if the MC1R gene is associated with coat color in water buffalo,the coding region of MC1R gene of 216 buffalo samples was sequenced,which included 49 black river buffalo (Murrah and Nili-Ravi),136 swamp buffalo (Dehong,Diandongnan,Dechang,Guizhou,and Xilin) with white and gray body,and 31 hybrid offspring of river buffalo Nili-Ravi (or Murrah) and swamp *** the three variation sites found,SNP684 was synonymous,while SNP310 and SNP384 were nonsynonymous,leading to p.S104G and p.I128M changes,*** Individuals carrying homozygote EBR/EBR were *** genotype and phenotype analysis of the hybrid offspring of black river buffalo and gray swamp buffalo further revealed that the river buffalo type allele EBR or the allele carrying the amino acid p.104S was important for the full function of *** in silico functional analysis showed that the amino acid substitutions p.G104S and p.M128I had significant impact on the function of *** results indicate that the allele EBR or the allele carrying the amino acid p.104S was associated with the black coat color in buffalo.

关键词： Buffalo coat color MC1R