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Spread of *** Aerosols Carrying Antibiotic-Resistant Genes （Including MRSA） from Chicken Farms to Their Neighborhood Residents Environment

Spread of *** Aerosols Carrying Antibiotic-Resistant Genes ...

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2.12.环境污染与大众健康学术会议

作者： Dunjiang Liu 1 Tongjie Chai 1, * Yumei Cai 1 Lingyu Sun 1 Haiyu Hao 1 Jian Wang 2.1 College of animal Science and veterinary Medicine, {2. Agricultural university, Sino-German Cooperative Research Centre for Zoonoses of animal Origin of shandong province, Tai’an, 2.1018, china.state environmental protection key laboratory of microorganism application and risk control, tsinghua university, 100084.Beijing, china. 2.bureau of animal husbandry and veterinary zouping county, shandong province, china.

There is a rather limited understanding concerning the antibiotic-resistance of the airborne S. aureus and the transmission of the antibiotic-resistant genes it carries Therefore, we isolated 149 S. aureus strains from the samples collected from the feces, the indoor air and the outdoor air of 6 chicken farms, and performed the research on them with 15 types of antibiotics and the REP-PCR trace identification. The 100% homologous strains were selected to conduct the research on the carrying and transmission status of the antibiotic-resistant genes. The results revealed that 5.37% strains（8/149） were resistant to methicillins （MRSA）, and 94% strains （140/149） were resistant to compound sulfamethoxazole, etc. In addition, these strains displayed a resistance to multiple antibiotics （4, 5 or 6 types） and there were also 3 strains resistant to 9 antibiotics. the strains with the same REP-PCR trace identification result carried the same type of antibiotic-resistant genes. The results showed that airborne transmission not only causes the spread of epidemic diseases but also exerts threats to the public health of a community.

关键词： chicken farming environment aerosols of Staphylococcus aureus including MRSA REP-PCR tracing antibiotic-resistance antibiotic-resistant genes significance to epidemiology and public health.

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Origin and Spread Identification of Escherichia Coli Aerosol of Swine House Surroundings Using ERIC-PCR and REP-PCR

Origin and Spread Identification of Escherichia Coli Aerosol...

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2.12.环境污染与大众健康学术会议

作者： Huiyong Duan 1,2., Tongjie Chai 1 , Dunjiang Liu 1 1 College of animal Science and veterinary Medicine, {2. Agricultural university, Sino-German Cooperative Research Centre for Zoonoses of animal Origin of shandong province, Tai’an, china. 2.1018 state environmental protection key laboratory of microorganism application and risk control, tsinghua university, Beijing, china. 100084.2.College of animal {12. and veterinary Medicine, Jining Agricultural School, Jining, {2., china. 2.2.00

Evidence is mounting that microorganisms originating from livestock impact the air quality of the animal houses themselves and contribute to their neighborhood of the public. The aim of this study was to develop efficient bacterial source tracking capabilities to identify sources of Escherichia coli aerosol pollution caused by pigs. Airborne E. coli were isolated from indoor air, upwind air （10 and 50 m away） and downwind air samples （10, 50, 100, 2.0 and 400 m away） for five swine houses using six-stage Andersen microbial samplers and RCS （Reuter-Centrifugal samplers）. E. coli strains from pig fecal samples were also collected simultaneously. The enterobacterial repetitive intergenic consensus polymerise chain reaction （ERIC-PCR） and the repetitive extragenic palindromic （REP-PCR） approaches were used to study the genetic variability and to determine the strain relationships among E. coli isolated from different sites in each swine house. Results showed that 35.1% （2./57） of the bacterial DNA fingerprints from the fecal isolates matched with the corresponding strains isolated from indoor and downwind air samples （similarity ≥ 90%）. E. coli strains from the indoor and downwind air samples were closely related to the E. coli strains isolated from feces, while those isolated from upwind air samples （house C） had low similarity （61% - 69%）. Our results suggest that some strains isolated from downwind and indoor air originated in the swine feces. Effective hygienic measures should be taken in animal farms to prevent or minimize the downwind spread of microorganism aerosol.

关键词： Source identification Swine house Airborne Escherichia coli ERIC-PCR REP-PCR.

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