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Influence of Cell Confluency on the Expression of the α4 Integrin Subunit of Retinal Pigment Epithelial Cells

Advances in Biological Chemistry 2015年第2期5卷 73-82页

作者： Jean-Michel Bourget Mohib Morcos Karine Zaniolo Sylvain L. Guérin stéphanie Proulx Centre de recherche du CHU de Québec and Centre de recherche en organogénèse expérimentale de l’Université Laval/LOEX axe médecine régénératrice Hopital du Saint-Sacrement Québec Canada Département d’anatomie et de cytologie pathologiques CHU de Québec Hopital du Saint-Sacrement Québec Canada Département de biologie moléculaire biochimie médicale et pathologie Faculté de médecine Université Laval Québec Canada Département d’Ophtalmologie Faculté de médecine Université Laval Québec Canada

Integrins are a family of transmembrane glycoproteins that mediate cell-cell and cell-extracellular matrix interactions. The integrin α4 subunit is widely expressed by cells from the immune system and its expression by non-hematopoietic cells is scarce. In the present study, gene and protein expression of this integrin subunit was characterized in proliferating and quiescent human RPE cells. Immunofluorescent studies confirm that the α4 subunit is expressed in vitro by RPE cells, a result that has been validated by immunofluorescence and FACS analyses. The accumulation of the α4 integrin at cell-cell junctions in post-confluent RPE cell cultures negatively correlated with the level of expression of the mRNA transcript. Accordingly, transient transfection analyses reveal that the α4 promoter activity is considerably reduced when RPE cells form a confluent monolayer. Moreover, transfection of recombinant constructs bearing 5’-deletions of the α4 promoter segment allows the localization of strong negative regulatory elements on the -76 to -300 region of the α4 gene suggesting that its expression is intimately linked to the proliferative state of primary cultured RPE cells.

关键词： Retinal Pigment Epithelium Integrin Alpha 4 Subunit Cell Culture Confluency Promoter

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估算初级端稳压恒流反激式转换器的输出电流容限(第2部分)

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中国集成电路 2018年第7期27卷 60-64页

作者： stéphanie Cannenterre 安森美半导体

1前言本文的第1部分介绍了初级端稳压恒流反激式转换器的分析模型。我们也考虑了通过与实验结果进行对比来验证这个模型的有效性。该模型将使我们能够确定在LED驱动器应用中,在最差的情况下能够实现的反激式输出电流的精度。在第2部分中... 详细信息

1前言本文的第1部分介绍了初级端稳压恒流反激式转换器的分析模型。我们也考虑了通过与实验结果进行对比来验证这个模型的有效性。该模型将使我们能够确定在LED驱动器应用中,在最差的情况下能够实现的反激式输出电流的精度。在第2部分中,我们将通过执行蒙特卡罗分析来估算转换器在最差情况下的输出电流极限。

关键词：输出电流转换器反激式恒流稳压估算容限 LED驱动器

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Liver transplantation for alcoholic liver disease: Lessons learned and unresolved issues

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World Journal of Gastroenterology 2015年第39期21卷 10994-11002页

作者： José Ursic-Bedoya stéphanie Faure Hélène Donnadieu-Rigole Georges-Philippe Pageaux Liver Transplantation Unit Digestive Department Saint Eloi University Hospital University of Montpellier Addictology Department Saint Eloi University Hospital University of Montpellier

The use of liver transplantation(LT) as a treatment for alcoholic liver disease(ALD) has been highly controversial since the beginning. The ever increasing shortage of organs has accentuated the low priority given to patients suffering from ALD, which is considered a "self-inflicted" condition. However, by improving the long-term survival rates, making them similar to those from other indications, and recognizing that alcoholism is a primary disease, ALD has become one of the most common indications for LT in Europe and North America, a situation thought unfathomable thirty years ago. Unfortunately, there are still many issues with the use of this procedure for ALD. There are significant relapse rates, and the consequences of excessive drinking after LT range from asymptomatic biochemical and histological abnormalities to graft failure and death. A minimum three-month period of sobriety is required for an improvement in liver function, thus making LT unnecessary, and to demonstrate the patient's commitment to the project, even though a longer abstinence period does not guarantee lower relapse rates after LT. Recent data have shown that LT is also effective for severe alcoholic hepatitis when the patient is unresponsive to corticosteroids therapy, with low relapse rates in highly selected patients, although these results must be confirmed before LT becomes a standard procedure in this setting. Finally, LT for ALD is accompanied by an increased risk of de novo solid organ cancer, skin cancer, and lymphoproliferative disorders, which has a large impact on the survival rates.

关键词： Liver transplantation Alcoholic liver disease Cirr

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Minimally Invaded Sentinel Lymph Node Model for the Development of Intraoperative Infrared Fluorescence Imaging

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Advances in Molecular Imaging 2018年第4期8卷 48-58页

作者： Badiane Serigne Moussa Raes Florian Julien Sobilo Natkunarajah Sharuja Lerondel stéphanie Le Pape Alain UFR des Sciences de la Santé Université Gaston BERGER Saint-Louis Sénégal Centre for Small Animal Imaging PHENOMIN-TAAM CIPA CNRS UPS44 Orléans France

In this study we implemented an axillary SLN invasion model to develop highly sensitive imaging strategies enabling detection of a very small amount of tumor cells. A highly diffusible molecular probe targeting αvβ3 and αvβ5 integrins was investigated either via IV or locoregional injections. We additionally documented the potential interferences of this Near Infrared Fluorescence Probe with Blue Patente V and ICG dyes routinely used to facilitate lymph node detection during surgery. The human mammary adenocarcinoma MDA-MB-231-luc model was injected into the forepaw of nude female rats to obtain a controlled invasion of the axillary LN. Thanks to its high sensitivity, BLI was selected to achieve in vivo quantitation of tumor cells in SLNs and determine eligible animals for the study. NIRF of integrins was performed at 680 nm both in vivo and ex vivo using spectral unmixing to suppress auto-fluorescence signal and preserve sensitivity. In vivo BLI was quite reliable in estimating discrete invasion by cancer cells in the LN with thresholds of detection and quantitation of about 500 and 1500 cells respectively. For fluorescence at 680 nm, in vivo imaging is not suitable to detect micro-invasion, but ex vivo fluorescence with spectral unmixing of SLNs confirmed the presence of a tumor burden as low as 1500 cells expressing αvβ3/αvβ5 integrins. Targeting few tumor cells inside a micro-invaded sentinel lymph node by molecular probes is not sensitive enough to provide direct in vivo or peroperative imaging. At the time NIRF is performed on the excised specimen, high sensitivity imaging associated with spectral unmixing allowed such detection within less than 1 minute of examination.

关键词： Sentinel Lymph Node Near Infrared Fluorescence Imaging Photoacoustic Imaging Molecular Imaging

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A PERTURBATION METHOD FOR THE NUMERICAL SOLUTION OF THE BERNOULLI PROBLEM

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Journal of Computational Mathematics 2008年第1期26卷 23-36页

作者： Franois Bouchon stéphane Clain Rachid Touzani Laboratoire de Mathématiques Universite Blaise Pascal (Clermont-Ferrand) and CNRS (UMR 6620) Campus Universitaire des Cézeaux63177 Aubière cedexFrance Laboratoire MIP UFR MIGUniversité Paul Sabatier Toulouse 3118 route de Narbonne31062 Toulouse cedex 4France Laboratoire de Mathematiques Université Blaise Pascal (Clermont-Ferrand) and CNRS (UMR 6620) Campus Universitaire des Cézeaux63177 Aubière cedexFrance

We consider the numerical solution of the free boundary Bernoulli problem by employing level set formulations. Using a perturbation technique, we derive a second order method that leads to a fast iteration solver. The iteration procedure is adapted in order to work in the case of topology changes. Various numerical experiments confirm the efficiency of the derived numerical method.

关键词： Bernoulli problem Free boundary Level sets

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Impact of the arterial input function on microvascularization parameter measurements using dynamic contrast-enhanced ultrasonography

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World Journal of Radiology 2012年第7期4卷 291-301页

作者： Marianne Gauthier stéphanie Pitre-Champagnat Farid Tabarout Ingrid Leguerney Mélanie Polrot Nathalie Lassau IR4M-UMR 8081 Institut Gustave Roussy 94805 Villejuif cedex France Service Commun d'Expérimentation Animale Institut Gustave Roussy 94805 Villejuif cedex France Institut de recherche en cancérologie à Villejuif Institut Gustave Roussy 94805 Villejuif cedex France

AIM: To evaluate the sources of variation influencing the microvascularization parameters measured by dynamic contrast-enhanced ultrasonography (DCE-US). METHODS: Firstly, we evaluated, in vitro , the impact of the manual repositioning of the ultrasound probe and the variations in flow rates. Experiments were conducted using a custom-made phantom setup simulating a tumor and its associated arterial input. Secondly, we evaluated, in vivo , the impact of multiple contrast agent injections and of examination day, as well as the influence of the size of region of interest (ROI) associated with the arterial input function (AIF). Experiments were conducted on xenografted B16F10 female nude mice. For all of the experiments, an ultrasound scanner along with a linear transducer was used to perform pulse inversion imaging based on linear raw data throughout the experiments. Semi-quantitative and quantitative analyses were performed using two signal-processing methods. RESULTS:In vitro , no microvascularization parameters, whether semi-quantitative or quantitative, were significantly correlated (P values from 0.059 to 0.860) with the repositioning of the probe. In addition, all semiquantitative microvascularization parameters were correlated with the flow variation while only one quantitative parameter, the tumor blood flow, exhibited P value lower than 0.05 (P = 0.004). In vivo , multiple contrast agent injections had no significant impact (P values from 0.060 to 0.885) on microvascularization parameters. In addition, it was demonstrated that semi-quantitative microvascularization parameters were correlated with the tumor growth while among the quantitative parameters, only the tissue blood flow exhibited P value lower than 0.05 (P = 0.015). Based on these results, it was demonstrated that the ROI size of the AIF had significant influence on microvascularization parameters: in the context of larger arterial ROI (from 1.17 ± 0.6 mm 3 to 3.65 ± 0.3 mm 3 ), tumor blood flow and

关键词： Dynamic contrast-enhanced ultrasonography Angiogenesis Linear raw data Arterial input function Functional imaging

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通过解决营养和体育活动障碍来实现高血压预防与进展的公平性设计:一项聚类随机试验

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中华高血压杂志(中英文) 2024年第2期32卷 200-200页

作者： Hardy st Hannon L Hall L Cherrington AL 刘青练桂丽

美国南部农村地区的高血压和贫困率较高,导致非洲裔成年人心血管疾病发病率高于白人成年人的健康群体,凸显确定预防策略的必要性。方法和结果:通过解决营养和体育活动障碍来实现高血压预防和进展的公平性(equity in prevention and prog...

美国南部农村地区的高血压和贫困率较高,导致非洲裔成年人心血管疾病发病率高于白人成年人的健康群体,凸显确定预防策略的必要性。方法和结果:通过解决营养和体育活动障碍来实现高血压预防和进展的公平性(equity in prevention and progression of hypertension by addressing barriers to nutrition and physical activity,EPIPHANY)研究是一项聚类随机对照试验,测试一种多层次干预措施,以减少农村非洲裔成人健康生活方式障碍,从而降低血压。

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The undetected error probabilities of combinatorial codes and their dual codes

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Chinese Science Bulletin 1997年第23期42卷 2017-2018页

作者： Xia, st Fu, FW Nankai Univ Dept Math Tianjin 300071 Peoples R China

IT is known that the average undetected error probability （UEP） of a binary [n, k] code Con a binary symmetric channel with crossover probability p is given byPe（p） = sum from i=1 Aipi（1-p）n-1, （1）where （A0, A1,…, An） is the weight distribution of C. If for all 0≤p≤0.5, Pe（p）≤Pe（0. 5） = 2k-n-2-n, then C is called good for error detection. Moreover, if Pe （p） ismonotonously increasing in the interval [0, 0.5], then C is called proper. Clearly, propercodes are good.

关键词： UEP The undetected error probabilities of combinatorial codes and their dual codes code

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Error-detecting abilities of binary nonlinear constant weight codes

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Chinese Science Bulletin 1997年第4期42卷 347-350页

作者： Fu, FW Xia, st Department of Mathematics Nankai University Tianjin 300071 China

CONstANT weight codes are very important in coding theory for its application to communication. In refs. [1—5], the undetected error probabilities (UEP)of binary nonlinear constant weight codes (BNCW) were studied, and Wang et al. showed that when n>8, BNCW codes (n, 2, ω) were not proper, Wang proposed a conjecture that when n>4δ and δ> 1, BNCW codes (n, 2δ, ω) are not proper. in this note, the error-detecting abilities

关键词： nonlinear constant weight code optimum constant weight code.

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Role of human epidermal growth factor receptor 2 in gastric cancer:Biological and pharmacological aspects

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World Journal of Gastroenterology 2014年第16期20卷 4526-4535页

作者： Jan Tr?st J?rgensen Dx-Rx Institute 3480 FredensborgDenmark

Amplification of the human epidermal growth factor receptor 2 (HER2) gene and overexpression of the HER2 protein is found in 15%-20% of patients with gastric and gastroesophageal junction cancer. The degree of HER2 overexpression and amplification varies with the location of the carcinoma, with higher expression in the gastroesophageal and proximal parts compared to the distal parts of the stomach. Further, HER2 overexpression and amplification also seems to be related to the Lauren histological classification, with higher levels found in the intestinal phenotype compared to the diffuse and mixed types. The prognostic properties of HER2 overexpression and amplification are still under debate, but a large number of studies seem to indicate that HER2 is a negative prognostic factor. The usefulness of HER2 targeted therapy in gastric cancer was demonstrated in the ToGA trial, where HER2-positive patients with advanced gastric and gastroesophageal junction adenocarcinoma were randomized to receive 5-FU/capecitabine and cisplatin, either alone or in combination with trastuzumab. A statically significant gain in overall survival was seen in patients who received the combined treatment of trastuzumab and chemotherapy. Patients with a strong overexpression of the HER2 protein (IHC3+) specifically benefited from the treatment, with a median overall survival of 17.9 mo. As a consequence of the positive results of the ToGA trial, patients with advanced gastric or gastroesophageal junction adenocarcinoma are now routinely tested for HER2. The ToGA trial must be characterized as a landmark in the treatment of gastric cancer and it has paved the way for a number of new HER2 targeted compounds such as pertuzumab, ado-trastuzumab emtansine, lapatinib, afatinib, and dacomitinib, which are currently undergoing phase II and III clinical testing. Overall, this review will discuss the current status of HER2 in gastric and gastroesophageal junction cancer and the future direction in rela

关键词： Human epidermal growth factor receptor 2 Gastric cancer Prognostic Companion diagnostics Trastuzumab Pertuzumab Ado-trastuzumab emtansine Lapatinib

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