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Population structure analysis and determ.nation of neurotoxin content in a set of grass pea (Lathyrus sativus L.) accessions of Bangladesh origin

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The Crop Journal 2018年第4期6卷 435-442页

作者： Priyanka Gupta sripada m. udupa Debjyoti Sen Gupta Jitendra Kum.r Shiv Kum.r International Center for Agricultural Research in the Dry Areas Rabat Institute Rabat Morocco Departm.nt of Plant Sciences North Dakota State University Fargo ND 58105 USA ICAR-Indian Institute of Pulses Research Kanpur Uttar Pradesh 208024 India

Grass pea(Lathyrus sativus L.) is a crop that is considered one of the m.re resilient to clim.te change. With protein-rich seeds and leaves, it has strong potential as hum.n food as well as anim.l feed and fodder. However, genetic im.rovem.nt in this crop rem.ins stagnant owing to the poor characterization of its genetic resources. In this study, we characterized 118 accessions of grass pea with 18 EST-SSR m.rkers. A total of 118 accessions, 101 of L. sativus(100 cultivated accessions from.Bangladesh and one wild accession) and 17 wild accessions of other Lathyrus species, were used. A total of 67 alleles were detected, with an average of 3.72 alleles per locus and average polym.rphism.inform.tion content of 0.52. A dissim.larity m.trix was form.d and hierarchical cluster analysis perform.d using the UPGm. m.thod grouped genotypes into four m.in clusters. Cluster analysis based on the genetic dissim.larity revealed a clear grouping of the 100 cultivated and 18 wild accessions into four m.in groups. Group I consisted of 20 accessions with high β-N-oxalyl-L-α,β-diam.nopropionic acid(β-ODAP) concentration. Of these 20 accessions, 17 were wild accessions. Only one wild accession(L. cicera) was clustered in group II, which contained 35 accessions. m.st of the group II accessions contained low β-ODAP. Group III was represented by 34 accessions, m.ny of them.with high β-ODAP. Group IV consisted of 29 accessions, a few of which had very high β-ODAP concentrations. Analysis of m.lecular variance of the m.crosatellite data showed significantly higher values of m.lecular variance between(83%) than within(17%) populations. These characterized accessions will be useful in grass pea breeding program..

关键词： Lathyrus Population structure EST-SSRβ-ODAP Genetic diversity

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Beneficiation of a low grade lim.stone sam.le

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m.ning Science and Technology 2011年第5期21卷 631-636页

作者： Rao Danda Srinivas Vijayakum.r Tadiparthi Venkata Subba Rao sripada Bhaskar Raju Guntam.dugu Prabhakar Swarna National m.tallurgical Laboratory m.dras Centre CSIR Madras Complex Chennai 600 113 India

Pilot scale colum. flotation studies were conducted on a low grade siliceous lim.stone ore. Silica content was reduced to less than 13g in the concentrate so that it becam. satisfactory for use in the paper or rubber industries. The lim.stone sam.le was crystalline and constituted prim.rily of calcite that contained quartz, feldspar, pyroxene, and biotite as gangue m.nerals. Quartz is the m.jor silicate gangue whereas feldspar, pyroxene, and biotite exist in m.nor to trace quantities. Traces of pyrite were also observed within the sam.le. A reverse flotation process was adopted where the silicate gangue m.nerals were floated using two different com.ercial cationic collectors： Chem.750 F or Floatam.ne-D. The studies clearly suggest it is possible to produce a lim.stone concentrate assaying around 96-97% CaCO3 containing less than 1 % Si02. The effect of feed flow rate, percent solids, froth depth, and wash water on the grade and recovery of the CaC03 concentrate is discussed.

关键词： Low grade lim.stoneReverse flotationColum. flotationCationic collector

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