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The Effect of Salinity on Growth, Dry Weight and Lipid Content of the Mixed Microalgae Culture Isolated from Glagah as Biodiesel Substrate

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Journal of Life Sciences 2015年第5期9卷 229-233页

作者： Eko agus Suyono Winarto Haryadi Muhammad Zusron Matin Nuhamunada sri Rahayu Andhika Puspito Nugroho Faculty of Biology Universitas Gadjah Mada Yogyakarta 55281 Indonesia Department of Chemistry Faculty of Mathematics and Natural Sciences Universitas Gadjah Mada Yogyakarta 55281 Indonesia

Microalgae use photosynthesis to convert solar energy into chemical energy, such as lipid and they can be a replacement for oil-based fuels. They are among the fastest growing plants in the world, and about 50% of their weight is oil. This lipid oil can be used to make biodiesel. Unfortunately, there are only some of potential strains isolated from Indonesia and most of the biodiesel productions are usually using a single strain. Then, although they are rich of oils, their biomass productivity is still low. Salinity treatment can be used to increase their biomass as well as their lipid content. Therefore, the research aim was to study the effect of salinity on the growth, dry weight and lipid content of mixed microalgae isolated from Glagah, Yogyakarta. The mixed microalgae were cultured in 3NBBM medium with different salinities or types of water （sea water, brackish water, and fresh water）. The cultures were incubated at light intensity 3,000 lux under dark：light exposure of 12：12 h for 7 days. The number of cells was counted every 24 h with a Haemocytometer, and the biomass was calculated based on the dry weight. The lipid content was measured on days 0, 3, and 7 using NR （Nile Red） staining, and then the amount of lipid was analyzed using a fluorescence microscope and measured with CellProfiler 2.0 software. The highest dry weight and lipid content were found in seawater medium, they accounted for 3.42 mg/mL and 13.58% at day 7, respectively. Whereas, the highest number of cells was found in freshwater medium, this was 9.8 × 10^6 cells/mL.

关键词： Salinity growth dry weight lipid content.

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Knowledge and Practice on Tuberculosis among Prison Workers from Seremban Prison

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Occupational Diseases and Environmental Medicine 2019年第4期7卷 176-186页

作者： G. Selvaganapathi Jinat Ahmed A. G. Mathialagan M. Dinesh N. Azra T. Harikrishnan S. Vanita M. N. M. Nazmul agus Iwan Foead Seremban Prison Malaysian Prison Department Home Ministry Putrajaya Malaysia Perdana University Graduate School of Medicine Perdana Malaysia Perdana University Royal College of Surgeons Ireland Serdang Malaysia SP Care Group Health Care Rawang Malaysia Department of Rehabilitation Medicine Hospital Tunku Ampuan Rahimah Klang Malaysia Klinik sri Pulai Sdn Bhd Seremban Malaysia

Background: Prisons are always overcrowded, and congestion leads to close relationships, and subsequently, increases the likelihood of airborne illnesses including Tuberculosis (TB). The purpose of this study was to identify the level of knowledge of TB transmission, and TB preventive practices of prison workers in a Malaysian prison. Methods: A cross-sectional study was conducted with convenience sampling technique utilizing a validated questionnaire. 280 prison workers employed at the Seremban Prison were invited to participate in the study. The study period was from January 2019 to June 2019. Inclusion criteria included age above 18 years old and English literacy. The questionnaire was divided into 3 parts which were sociodemographic details, knowledge on TB transmission and TB preventive practices. The analysis was done in SPSS version 24.0 using appropriate statistical tests. Descriptive statistics was used to evaluate the knowledge level of TB transmission, and TB preventive practices of prison workers at Seremban prison. Results: Around 190 questionnaires were obtained usable registering a response rate of 68.5%. This study showed that around 66% of prison workers had moderate knowledge while around 26% had good knowledge of TB transmission. In terms of practice, around 93.7% of prison workers applied good TB prevention practice. Conclusion: This study showed that the majority of the respondents had moderate knowledge regarding TB transmission. However, their disease preventive practices were at acceptable levels which implied that existing prison standard operating procedures play an important on instilling these practices. Nonetheless, prison management should take important action by providing education and training to increase TB knowledge among prison workers to ensure that this practice is always upheld in both prison and community settings.

关键词： Knowledge Practice Prevention Tuberculosis Prison Worker Prison Malaysia

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