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Multi-gene-based investigation on the molecular phylogeny of the hypotrichous family strongylidiidae(Protista,Ciliophora),with notes on the ontogeny of a new genus and new species

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Marine Life science & Technology 2024年第3期6卷 442-461页

作者： Wenya s.ng shijing Zhang Yuqing Li Honggang Ma Qiyu Li Xiaotian Luo Khaled A.s.Al-Rasheid Hunter n.Hines Xiaoteng Lu Institute of Evolution&Marine biodiversity Ocean University of ChinaQingdao 266003China Key Laboratory of Aquatic biodiversity and Conservation of Chinese Academy of sciences Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China Weishan special Aquaculture base Jining 277600China Zoology Department King Saud University11451 RiyadhSaudi Arabia Harbor branch Oceanographic Institute Florida Atlantic UniversityFort PierceFlorida 34946USA Department of biology Shenzhen MSU-BIT UniversityShenzhen 518172China

Ciliates in the subclass Hypotrichia have long been difficult to classify as they are one of the most polymorphic and highly differentiated groups,leading to their systematics remaining *** relationships within the hypotrich family strongylidiidae have been ambiguous due to discordance between the morphological and genetic *** this study,a new strongylidiid genus Heterouroleptus is established,mainly based on the novel mode of origin of the ventral cirral rows:left ventral cirral row(LVR)originates from frontal-ventral-transverse cirral anlagen(FVTA)Ⅲ(anterior portion),IV(middle portion),and V(rear portion);right ventral cirral row comes from the entire FVTA VI.A new species,Hetero-uroleptus weishanensis ***.,***.,is investigated along with the morphometric and molecular data from a population of strongylidium *** new sequences and nuclear gene markers(s.ngle-gene and multi-gene)are provided to analyze the phylogenetic relationships of strongylidiids,with the COI gene utilized to uncover further genetic information at species level and *** results reveal that:(1)strongylidiidae is monophyletic and has a close relationship with Dorsomarginalia;(2)Heterouroleptus *** a clade that is sister to all the other strongylidiids;(3)Hemiamphisiella Fois.ner,1988 and Pseudouroleptus Hemberger,1985 should not be s.nonyms,and both genera should be subdivided due to their variable morphological characteristics;(4)LVR originating from three anlagen is a plesiomorphy of *** discovery of the origin of the LVR not only contributes to the establishment of the genus Heterouroleptus,but also helps to improve the diagnosis of the family strongylidiidae.

关键词： Ciliates Cytochrome c oxidase subunit I gene Morphogenesis Phylogeny·Ribosomal RnA gene Taxonomy

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Proximity to corridors benefits bird communities in vegetated interrow vineyards in Mendoza, Argentina

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Avian Research 2024年第2期15卷 147-155页

作者： Andrea Paula Goijman Agustín Zarco EEA La Consulta Instituto Nacional de Tecnología Agropecuaria(INTA)San CarlosMendozaArgentina Instituto Argentino de Investigacion en las Zonas Aridas(IADIZA)COnICET 5500MendozaArgentina

Management under ecological schemes and increas.ng habitat heterogeneity,are ess.ntial for enhancing biodiversity in *** provide several contributions to agriculture,for example pest control,recreation and enhancing human mental health,and have intrinsic *** are also ideal model organisms because they are easy to survey,and species respond differently to agricultural land use at different *** borders of crops are key for many species of birds,and distance to the border have been found to be an important factor in vineyard-dominated *** evaluate if there are differences in the bird assemblage,between the interior compared to borders within vineyards,us.ng a hierarchical community occupancy *** hypoth-esized that occupancy of birds is greater in environments with greater heterogeneity,which in this study was considered to be contributed by the proximity to vegetated *** expected that vineyard borders close to corridors will have higher bird occupancy than the center of the *** research was conducted in three vineyards with biodiversity-friendly management practices,in Gualtallary,Mendoza,*** surveys were conducted over three breeding seas.ns from 2018 to *** and richness of the bird community was more closely associated with the borders adjacent to the corridors than with the interior of the vineyards,as we initially predicted,although the assemblage of birds did not differ *** than 75%of the registered species consume exclusively or partially ***-friendly management and ecological schemes,together with vegetated corridors provide multiple benefits for biodiversity *** ap-proaches.not only minimize the use of agrochemicals but also prioritize soil cover with spontaneous vegetation,which supports a diverse community of insectivorous bird species,potentially contributing to pest control.

关键词： Avian bayesian analysis Field scale agroecosystem Management Multi-species occupancy model nature’s contributions to people

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Elucidating elusive quaternary selenide EuCeCuse3:s.nthesis,crystal structure,properties and theoretical studies

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Journal of Rare Earths 2024年第1期42卷 163-171,I0006页

作者： Maxim V.Grigoriev Anna V.Ruseikina Maxim s.Molokeev Vladimir A.Chernyshev Aleks.ndr s.Aleks.ndrovsky Alexander s.Krylov svetlana n.Krylova nikolai P.shestakov Dmitriy A.Velikanov Alexander A.Garmonov Alexey V.Matigorov Evgeny A.Ostapchuk Thomas schleid Damir A.safin Laboratory of Theory and Optimization of Chemical and Technological Processes University of TyumenVolodarskogo Str.6625003TyumenRussian Federation Kirensky Institute of Physics Federal Research Center KSC SB RASAkademgorodok 50 Bld.38660036KrasnoyarskRussian Federation Department of Physics Far Eastern State Transport UniversitySerysheva Str.47680021KrasnoyarskRussian Federation Institute of natural sciences and Mathematics Ural Federal University Named After the First President of Russia B.N.YeltsinMira Str.19620002EkaterinburgRussian Federation Department of Photonics and Laser Technology Siberian Federal UniversitySvobodnii Ave.79660079KrasnoyarskRussian Federation Institute of Physics and Technology University of TyumenVolodarskogo Str.6625003 TyumenRussian Federation Institute for Inorganic Chemistry University of StuttgartPfaffenwaldring 55D-70569StuttgartGermany scientific and Educational and Innovation Center for Chemical and Pharmaceutical Technologies Ural Federal University named after the First President of Russia B.N.YeltsinMira Str.19620002 EkaterinburgRussian Federation University of Tyumen Volodarskogo Str.6625003TyumenRussian Federation

We report on the novel heterometallic quaternary selenide EuCeCuse3,the fabrication of which has been a challenge until this *** structure of the reported selenide was elucidated from the powder X-ray diffraction data,which revealed the formation of EuCeCuse3with excellent yield(96.7%)accompanied with a minor fraction of Cese2(3.3%),and was best solved in orthorhombic space group Pnma with the baLaCus3structural ***,the crystal structure of the title compound completes the row of the heterometallic quaternary selenides EuRECuse3(RE=La,Pr,nd,sm,Eu,Gd,Tb,Dy,Ho,Er,Tm,Yb,Lu,Y),of which the cerium-based derivative exclusively belongs to the baLaCus3structural *** distortion of the Cuse4polyhedron was compared for the whole series of EuRECuse3compounds us.ng theτ4-descriptor for four coordinated ions,which revealed the highest degree of distortion for the Ce3+-containing selenide,followed by the La3+-based ***,the crystallographic and geometrical parameters of the reported selenide were discussed in comparis.n to the Ce3+-based sulfides srCeCus3and *** initio calculations of the crystal structure,a phonon spectrum and elastic constants for the crystal of EuCeCuse3were also *** types and wavenumbers of fundame ntal modes were determined and the involvement of ions participating in the phonon modes was *** experimental IR spectrum of the reported selenide was interpreted and found to be in agreement with the calculated *** experimental direct band gap of EuCeCuse3was measured to be 1.36 eV that is consistent with the concept of its origin due to interband transitions between orbitals emerging mainly from 4f(valence band)and 5d(conduction band)levels of the Eu2+*** dependence of the Young’s modulus on the direction demonstrates the anisotropy of the elastic properties,while the Vickers hardness for EuCeCuse3was calculated to be 5.2 ***,the title compound is paramagnetic above 4 K.

关键词： Quaternary selenide s.nthesis Crystal structure Ab initio calculations Magnetic measurements spectroscopy

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Vegetation Response To soil And Morphological Properties In An Arid Region Alluvial Fan Landscape. Pre-Andes Of s.n Juan， Argentina

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Journal of Earth science 2024年

作者： FLOREs Daniel Germán OCA?A Raúl Emmanuel MELIán Edgardo ORTEG Andrés EsPER AnGILLIERI María Yanina PITTALUGA María Alejandra CIGEObIO (COnICET-UnsJ) Av.José I.de la Roza y Meglioli Gabinete de Geología Ambiental， InGEO， Universidad nacional de s.n Juan， Av.José I.de la Roza y Meglioli

The Monte Desert is characterized by a great diversity of landforms created with fluvial， alluvial which the vegetation patterns are related to. The pres.nt work has the following objectives: 1: determine whether topographical attributes， surface characteristics， soil properties and vegetation patterns vary between alluvial landforms， and 2: define whether morphometric， soil and surface properties influence vegetation patterns along alluvial landscape. Morphometric data were obtained by process.ng a 5 m digital elevation model. The coverage of rock fragments， fine sediments and mulch was quantified. Observations and descriptions of the soil profiles were restricted to the uppermost 50 cm. Vegetation properties were calculated us.ng a Point Quadrat Method. The relationship between variables was evaluated through multivariate statistical analysis. The main results show the pres.nce of 45 plant species distributed in 19 families， where shrubs are dominant. The wind effect， topographic wetness and dissection of the landscape are limiting factors of diversity. The coverage of superficial rock fragments influence vegetation coverage through the distribution and availability of rainwater. Furthermore， the different soil textures reveal that the silt content favors an increase in vegetation coverage. The pres.nce of V horizon could condition the installation and development of vegetation in the early stages of growth.

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Molecular characterization of an outbreak-involved bacillus anthracis strain confirms the spillover of anthrax from West Africa

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Infectious Diseases of Poverty 2024年第1期13卷 93-94页

作者： shuchao Wang Roland suluku Mohamed b.Jalloh Ahmed F.samba baogui Jiang Yubiao Xie Doris Harding Mengyao Zhang Foday sahr Mahmud E.sesay James s.squire Mohamed A.Vandi Moinina n.Kallon shoufeng Zhang Rongliang Hu Yuee Zhao Zhiqiang Mi Changchun Veterinary Research Institute Chinese Academy of Agricultural Sciences Changchun China Department of Animal sciences School of Agriculture and Food Sciences Njala University Njala Sierra Leone Department of Microbiology College of Medicine and Allied Health Sciences University of Sierra Leone Freetown Sierra Leone Ministry of Agriculture and Food sciences Freetown Sierra Leone beijing Institute of Microbiology and Epidemiology Beijing China Ministry of Health and s.nitation Freetown Sierra Leone beijing Institute of biotechnology Beijing China

background Anthrax,a zoonotic disease caused by the spore-forming bacterium bacillus anthracis,remains a major global public health concern,especially in countries with limited *** Leone,a West African country historically plagued by anthrax,has almost been out of report on this disease in recent *** this study,we described a large-scale anthrax outbreak affecting both animals and humans and attempted to characterize the *** The causative agent of the animal outbreak in Port Loko District,sierra Leone,between March and May 2022 was identified us.ng the nanopore sequencing technique.A nationwide active surveillance was implemented from May 2022 to June 2023 to monitor the occurrence of anthrax-specific symptoms in *** cases were subsequently verified us.ng quantitative polymerase chain ***-genome sequencing was accomplished by combining long-read and short-read sequencing *** phylogenetic analysis was performed based on the full-chromosome s.ngle nucleotide *** The outbreak in Port Loko District,sierra Leone,led to the death of 233 animals between March 26th and May 16th,*** ruled out the initial suspicion of Anaplasma species and successfully identified *** as the causative agent of the *** a result of the government's prompt response,out of the 49 suspected human cases identifed during the one-year active surveillance,only 6 human cases tested positive,all within the frst month after the official declaration of the *** phylogenetic analysis indicated that the basL2022 isolate responsible for the outbreak was positioned in the ***.153 clade within the TransEuroAsian group of *** We successfully identifed a large-scale anthrax outbreak in sierra *** causative isolate of ***,basL2022,phylogenetically bridged other lineages in ***.153 clade and neighboring genetic groups,***.144 and ***.148,

关键词： Molecular identification Anthrax bacillus anthracis sierra Leone Phylogeny s.ngle nucleotide polymorphism

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Advancements in assess.ng soil health through functional traits and energy flow analysis of soil

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soil Ecology Letters 2024年第2期6卷 21-30页

作者： Jingnan Zhang shiyu Li Elly Morrien neil b.McLaughlin shixiu Zhang school of Life sciences Zhengzhou UniversityZhengzhou 450001China state Key Laboratory of black soils Conservation and Utilization Northeast Institute of Geography and AgroecologyChineseAcademy of SciencesChangchun 130012China Key Laboratory of Mollisols Agroecology Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130012China Institute of biodiversity and Ecosystem Dynamics(IbED-ELD) Department of Ecosystem and Landscape DynamicsUniversity of AmsterdamScience Park 9041098 XHA msterdamThe Netherlands Ottawa Research and Development Centre Agriculture and Agri-Food CanadaOttawa K1AoC6Canada University of Chinese Academy of sciences Bejing 100049China

We examined the development of soil nematodes ecological indices from the perspective off *** found that soil nematode energy flow analyses based on multiple functional traits quantify the dynamics of energy flow across multipletrophic levels to provide a more comprehensive *** conducted comparative analyses of the s.nsitivities of nMF and energy flow to verify that the energy flow analyses are more s.nsitive and have greater potential to reveal soil health and ecosystem *** in-depth studies of functional traits and energy flow analysis can help us achieve informed soil management practices,sustainable agriculture,*** Tess CT nematode ecological index based on functional traits:MI El,s,bI,C1 nMF bongen.1990 Ferrisetal,2001 Ferris,2010 energs low analysis of soil nematodes Compare thecological index(nMf)and bacterivores(ba)Functional traits Energy flux Fungihores(Fn)rahn soilnematodes latitude Lindicator Plant-parasites(PP)PF Energflus soilhealth Omnivores-predators(oP)latitude Energy flow analysis of soil nematodes:by quantifying energy fux among trophic groups barbes et al.,2014,2018 This paper examines the development of ecological indices for soil nematodes from the perspective of functional *** emphasizes the increas.ng significance of integrating multiple functional traits to achieve a more accurate assessment of soil *** indices based on life history strategies,feeding habits,and body size provide useful tools for assess.ng soil ***,these indices do not fully capture the dynamics ofenergyflow across multiple-trophic levels inthesoil foodweb,which is critical fora deeperunderstanding of the intrinsic properties of soil *** combining functional traits such as functional group,body size,feeding preference and metabolic rate,nematode energy flow analyses provide a more comprehensive *** approach establishes a direct correlation between changes

关键词： soil nematodes soil health nematodefoodweb functional traits energyflux

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Omics-based biomarkers as useful tools in metabolic dysfunctionassociated steatotic liver disease clinical practice:How far are we?

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World Journal of Gastroenterology 2024年第14期30卷 1982-1989页

作者： Julieta Trinks María F Mascardi Adrián Gadano sebastián Marciano Instituto de Medicina Traslacional e Ingeniería biomédica-Consejo nacional de Investigaciones Científicas y Técnicas-Instituto Universitario del Hospital Italiano-Hospital Italiano de buenos Aires Ciudad Autónoma de Buenos Aires C1199ACLArgentina Consejo nacional de Investigaciones Científicas y Técnicas Ciudad Autónoma de Buenos Aires C1425FQBArgentina Liver Unit Hospital Italiano de Buenos AiresCiudad Autónoma de Buenos Aires C1199DFArgentina Department of Research Hospital Italiano de Buenos AiresCiudad Autónoma de Buenos Aires C1199DFArgentina

Unmet needs exist in metabolic dysfunction-associated steatotic liver disease(MAsLD)risk *** ability to identify patients with MAsLD with advanced fibrosis and at higher risk for adverse outcomes is still *** novel biomarkers could repres.nt a meaningful improvement to current risk *** this aim,omics technologies have revolutionized the process of MAsLD biomarker discovery over the past *** the research in this field is thriving,much of the publication has been haphazard,often us.ng s.ngle-omics data and specimen sets of convenience,with many identified candidate biomarkers but lacking clinical validation and *** we incorporate these biomarkers to direct patients’management,it should be considered that the roadmap for translating a newly discovered omics-based signature to an actual,analytically valid test useful in MAsLD clinical practice is rigorous and,therefore,not easily *** article pres.nts an overview of this area’s current state,the conceivable opportunities and challenges of omics-based laboratory diagnostics,and a roadmap for improving MAsLD biomarker research.

关键词： Metabolic dysfunction-associated steatotic liver disease non-alcoholic steatohepatitis biomarker Risk stratification Omics

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Long-term monitoring reveals the effect of precipitation and silviculture on nothofagus regeneration in northern Patagonia mixed forests

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Ecological Processes 2024年第02期13卷 103-117页

作者： Georgina sola Camila Mateo Alejandro Dezzotti Paula Marchelli Hernán Attis beltrán Renato sbrancia Luis Chauchard Marcelo González Pe?alba Martín Lara Verónica El Mujtar Consejo nacional de Investigaciones Científicas y Técnicas (COnICET ) As.ntamiento Universitario s.n Martín de los Andes (AUsMA-UnCo) Instituto de Investigaciones Forestales y Agropecuarias bariloche (IFAb) Instituto Nacional de Tecnología Agropecuaria (INTA) Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET ) Departamento de Conservación y Manejo Parque Nacional Lanín- Administración de Parques Nacionales (PNL-APN)

background Tree regeneration is a key component of resilience because it promotes post-disturbance recovery of forests. northwestern Patagonia from Argentina is occupied by nothofagus alpina（na）, n. obliqua（no）, and n. dombeyi（nd） forests that grow along an intense precipitation gradient, managed throughout shelterwood silvicultural system by technicians of the Lanin natural Reserve. The objective of this study was to evaluate the influence of seeding cuttings over the dynamics of nothofagus mixed forests across landscape（precipitation gradient） based mainly on the composition and abundance of tree regeneration, permanent sampling plots and generalized linear mixed models. In particular, we analysed:（i） the structure of sexual and asexual regeneration during nd > 20 years after harvest（the dynamics of managed forests）, and（ii） the structure of sexual regeneration in primary and managed forests after > 20 years of harvest（the effect of silviculture）.Results.nd was the most abundant species in the regeneration of managed forests during all periods in both sites despite its lower contribution to the adult cohort. During the 10–20 years period after harvest, the humid site exhibited higher regeneration density than the mesic site(120,000 and 6000 ind ha-1, respectively), and it decreased afterwards. The number of established regeneration（> 2 m height） was lower for na in the mesic site and for no in the humid site(0 and 57 ind ha-1, respectively). However, in comparis.n to no, na showed a higher number of sprouted stumps and sprouts per stump, and a higher sprout height in the mesic site. no exhibited higher sprout mortality in the humid site. Finally, the regeneration of primary forests showed lower density and height, and a more balanced composition than that of managed *** The silvicultural effects on the mixed forest regeneration dynamics was strongly influenced by the condition of sites. Therefore, management prescriptions should be adj

关键词： nothofagus alpina nothofagus obliqua nothofagus dombeyi basal sprouting shelterwood silvicultural system Ecological resilience

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Transcriptomic responses to drought stress in the Patagonian southern beech nothofagus alpina

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Ecological Processes 2024年第2期13卷 192-203页

作者： Rita Maria Lopez Laphitz María Verónica Arana s.ntiago Agustín Varela Leandro Aníbal becker Carolina soliani María Marta Azpilicueta Paula Marchelli nicolás bellora Instituto de Diversidad y Evolución Austral (CEnPAT COnICET ) Instituto de Investigaciones Forestales y Agropecuarias bariloche (IFAb InTA COnICET ) Instituto de Tecnologías.nucleares para la salud (COnICET )

background Deciphering the genetic architecture of drought tolerance could allow the candidate genes identification responding to water stress. In the Andean Patagonian forest, the genus.nothofagus repres.nts an ecologically relevant species to be included in different genomic studies. These studies are scarce in south American ecosystems however repres.nt an important source of genomic data in order to interpret future climate-change environment scenarios of these emblematic forests. Here, we achieved the assemblage of the transcriptome of n. alpina while searching for key genes of activated or suppressed metabolic pathways in response to drought *** De novo transcriptome assembly resulted in 104,030 transcripts. Following confirmation of drought conditions, based on reduction of leaf water potential and stomatal conductance, a differential gene expression analysis resulted in 2720 significantly expressed genes(1601 up-regulated and 1119 down-regulated). Enrichment analysis(over-repres.ntation analysis and gene set enrichment analysis) resulted in more than one hundred stress-responsive term ontologies(i.e. biological processes) and pathways. Terms such as response to abscisic acid and pathways such as plant hormone signal transduction or starch and sucrose metabolism were over-repres.nted. Protein–protein interaction assessment resulted in networks with significantly expressed top common hub gene clusters(e.g. planttype cell wall biogenesis among down-regulated or AbA-signalling among up-regulated). These networks evidenced important regulators at gene expression such as transcriptional *** Responses of n. alpina seedlings to drought stress were evidenced by the activation of several genes linked to GO biological processes and KEGG pathways, which were mainly based on over-expression of specific protein kinases, phosphatases, s.nthases and transcription factors. This suggests an up-regulation of signalling pathways, triggered through pla

关键词： Climate change Differential gene expression Hub genes Patagonia Temperate forest

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Psychological consequences of a diagnosis of infertility in men:a systematic analysis

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Asian Journal of Andrology 2024年第1期26卷 10-19页

作者： sarah n biggs Jane Halliday Karin Hammarberg Reproductive Epidemiology Murdoch Children's Research InstituteMelbourne 3052Australia Department of Paediatrics University of MelbourneMelbourne 3052Australia Public Health and Preventative Medicine Monash UniversityMelbourne 3004Australia Victorian Assisted Reproductive Treatment Authority Melbourne 3000Australia

Almost half of infertility cases involve male *** the consequence of a diagnosis of male infertility,as a sole or partial contributor to the couples'infertility,to the mental health of men is required to ensure clinical care meets their psychological *** aim of this systematic analysis was to s.nthesize the evidence regarding whether men diagnosed with male factor infertility experience greater psychological distress than(1)men described as fertile and(2)men in couples with other infertility *** databases were searched us.ng a combination of Medical subject Headings(MesH)headings and keywords relating to male infertility and psychological *** search yielded 1016 unique publications,of which 23 were included:8 case-control,14 prospective cohort,and 1 data linkage *** aspects of psychological distress were identified depression,anxiety,self-esteem,quality of life,fertility-related stress,general psychological stress or well-being,and psychiatric ***-control studies reported that men with male factor infertility have more symptoms of depression,anxiety and general psychological distress,worse quality of some aspects of life,and lower self-esteem than *** men with male factor infertility were compared to men in couples with other causes of infertility,there were few differences in the assessed aspects of psychological *** methodological limitations within the studies,this systematic analysis suggests that the experience of infertility,irrespective of its cause,negatively affects men's mental health and demonstrates the need for assisted reproduction technology(ART)providers to consider men undergoing assisted reproduction as individuals with their own unique support needs.

关键词： anxiety depression infertility male mental health quality of life

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