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Super-resolution morphological dissemination of intercalating dye in single DNA molecules via binding activated localization microscopy

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Chinese Chemical Letters 2018年第3期29卷 505-508页

作者： Guenyoung Park Suresh Kumar Chakkarapani soyeong ju Sujin Ahn Seong Ho Kang Department of Chemistry Graduate School Kyung Hee University Department of Applied Chemistry and Institute of Natural Sciences Kyung Hee University

Fluorescent dye （YOYO-I） intercalated with single DNA molecules were investigated via bindingactivated localization microscopy （BALM） at sub-diffraction limit resolutions. Various dye-to-DNA base pair （bp） ratios were imaged using the blinking property of YOYO-1 dye under optimum BALM switching buffer conditions. Individual DNA molecules exhibited regular/irregular intercalating phenomena with respect to dye-to-DNA bp ratio. The acquired images were reconstructed into super-resolution images by applying a Gaussian fit to the centroid of the point spread function. The YOYO-1 intercalated with λ-DNA possessed a non-homogeneous region due to the different binding modes of YOYO-1 with λ-DNA. Each binding mode was imaged at the sub-diffraction limit super-resolution. The distance between homogenously localized intercalating dyes within the DNA molecules was measured to be 34nm （n= 10; dye：DNAbp= 1：100） without photocleavage in 50mmol/L β-mercaptoethylamine buffer. The results were similar to those of the theoretical values without photocleavage in the base pairs of single DNA molecules below the diffraction limit. The results paved the way for an in-depth microscopic analysis of molecular variation with single λ-DNA molecules. With this method, it should be possible to analyze the exact base pair breakdown during various stages of cell apoptosis.

关键词： λ-DNA Intercalating dye Morphological dissemination Sub-diffraction limit resolution Binding-activated localization microscopy

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Ultra-flexible semitransparent organic photovoltaics

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npj Flexible Electronics 2023年第1期7卷 274-282页

作者： Hanbee Lee soyeong Jeong Jae-Hyun Kim Yong-Ryun Jo Hyeong ju Eun Byoungwook Park Sung Cheol Yoon Jong H.Kim Seung-Hoon Lee Sungjun Park Department of Electrical and Computer Engineering Ajou UniversitySuwon 16499Republic of Korea Department of Chemistry and Centre for Processable Electronics Imperial College LondonWhite City W120BZUK Department of Intelligence Semiconductor Engineering Ajou UniversitySuwon 16499Republic of Korea Advanced Photonics Research Institute(APRI) Gwangju Institute of Science and Technology(GIST)Gwangju 61005Republic of Korea Division of Molecular Science and Technology Ajou UniversitySuwon 16499Republic of Korea Division of Advanced Materials Korea Research Institute of Chemical Technology(KRICT)Daejeon 34114Republic of Korea Division of Advanced materials Engineering Kongju National University1223-24 Cheonan-daeroSeobuk-guCheonan-siChungcheongnam-doRepublic of Korea These authors contributed equally:Hanbee Lee Soyeong JeongJae-Hyun Kim

Ultra-flexible organic photovoltaics(OPVs)are promising candidates for next-generation power sources owing to their low weight,transparency,and ***,obtaining ultra-flexibility under extreme repetitive mechanical stress while maintaining optical transparency remains challenging because of the intrinsic brittleness of transparent ***,we introduce straindurable ultra-flexible semitransparent OPVs with a thickness below 2μ*** conformal surface coverage of nanoscale thin metal electrodes(justment of each ultrathin electrode thickness toward the emergence of next-generation flexible power sources.

关键词： transparency durability rigidity

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Wireless,multimodal sensors for continuous measurement of pressure,temperature,and hydration of patients in wheelchair

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npj Flexible Electronics 2023年第1期7卷 479-493页

作者： Seokjoo Cho Hyeonseok Han Hyunwoo Park Sung-Uk Lee Jae-Hwan Kim Sung Woo Jeon Mengqiu Wang Raudel Avila Zhaoqian Xi Kabseok Ko Minsu Park jungyup Lee Myungwoo Choi Je-Sang Lee Weon Gi Min Byeong-ju Lee soyeong Lee jungrak Choi Jimin Gu Jaeho Park Min Seong Kim junseong Ahn Osman Gul Chankyu Han Gihun Lee Seunghwan Kim Kyuyoung Kim Jeonghyun Kim Chang-Mo Kang Jahyun Koo Sung Soo Kwak Sungbong Kim Dong Yun Choi Seokwoo Jeon Hyung Jin Sung Yong Bae Park Minkyu Je Young Tae Cho Yong Suk Oh Inkyu Park Department of Mechanical Engineering Korea Advanced Institute of Science and TechnologyDaejeon 34141Republic of Korea School of Electrical Engineering Korea Advanced Institute of Science and TechnologyDaejeon 34141Republic of Korea Advanced 3D Printing Technology Development Division Korea Atomic Energy Research InstituteDaejeon 34057Republic of Korea Department of Electrical and Computer Engineering University of Illinois at Urbana-ChampaignUrbanaIL 61801USA State Key Laboratory of Structural Analysis for Industrial Equipment Department of Engineering MechanicsDalian University of TechnologyDalian 116023P.R.China Department of Mechanical Engineering Northwestern UniversityEvanstonIL 60208USA DUT-BSU Joint Institute Dalian University of TechnologyDalian 116023China Ningbo Institute of Dalian University of Technology Ningbo 315016China Department of Electronics Engineering Kangwon National UniversityChuncheon 24341Republic of Korea Querrey-Simpson Institute for Bioelectronics Northwestern UniversityEvanstonIL 60208USA Sibel Health Inc NilesIL 60714USA Department of Materials Science and Engineering KAIST Institute for The Nanocentury(KINC)Korea Advanced Institute of Science and TechnologyDaejeon 34141Republic of Korea Department of Rehabilitation Medicine Gimhae Hansol Rehabilitation&Convalescent HospitalGimhae 50924Republic of Korea Department of planning and development Gimhae Hansol Rehabilitation&Convalescent HospitalGimhae 50924Republic of Korea Department of rehabilitation medicine Pusan national university hospitalBusan 49241Republic of Korea Department of AI Convergence Network Ajou UniversitySuwon 16499Republic of Korea Department of Electronic Convergence Engineering Kwangwoon UniversitySeoul 01897Republic of Korea Korea Photonics Technology Institute Gwangju 61007Republic of Korea School of Biomedical Engineering Korea UniversitySeoul 02841Republic of Korea Interdisciplinary Program in Precision Public Health Korea UniversitySeoul 02841Republic of Korea Center

Individuals who are unable to walk independently spend most of the day in a *** population is at high risk for developing pressure injuries caused by ***,early diagnosis and prevention of these injuries still remain ***,we introduce battery-free,wireless,multimodal sensors and a movable system for continuous measurement of pressure,temperature,and hydration at skin *** device design includes a crack-activated pressure sensor with nanoscale encapsulations for enhanced sensitivity,a temperature sensor for measuring skin temperature,and a galvanic skin response sensor for measuring skin hydration *** movable system enables power harvesting,and data communication to multiple wireless devices mounted at skin-cushion interfaces of wheelchair users over full body *** evaluations and numerical simulations of the devices,together with clinical trials for wheelchair patients,demonstrate the feasibility and stability of the sensor system for preventing pressure injuries caused by sitting.

关键词： injuries modal mounted

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