检索结果-南通市图书馆

东莨菪亭(7-羟基-6-甲氧基香豆素)对7 ,12-二甲基苯并蒽诱导的皮肤乳头状瘤小鼠相关关键信号蛋白的影响(英文)

中西医结合学报 2010年第7期8卷 645-654页

作者： soumya Sundar Bhattacharyya Saili Paul Suman Dutta Naoual Boujedaini Anisur Rahman Khuda-Bukhsh Cytogenetics and Molecular Biology Laboratory Department of ZoologyUniversity of Kalyani Boiron Laboratory 20 rue de la LiberationSainte-Les-Foy-Lyon 69110France

目的:东莨菪亭(7-羟基-6-甲氧基香豆素)是从常绿钩吻中提取的有效成分,前期体外研究证实了其抗肿瘤潜能,本研究评价其在小鼠体内的抗肿瘤作用。方法:30只健康小鼠随机分为正常对照组、模型组、溶剂对照组和低、高剂量东莨菪亭组,每组6... 详细信息

目的:东莨菪亭(7-羟基-6-甲氧基香豆素)是从常绿钩吻中提取的有效成分,前期体外研究证实了其抗肿瘤潜能,本研究评价其在小鼠体内的抗肿瘤作用。方法:30只健康小鼠随机分为正常对照组、模型组、溶剂对照组和低、高剂量东莨菪亭组,每组6只。正常对照组小鼠不接受任何干预,其余小鼠背部涂抹100μg 7 ,12-二甲基苯并蒽(7 ,12-di methylbenz[a]anthra-cene ,DMBA)(每周一次)和1 %巴豆油(每周2次)诱导皮肤乳头状瘤,共24周。溶剂组小鼠在造模基础上每天予2 %酒精口服,低剂量(50 mg/kg)和高剂量(100 mg/kg)东莨菪亭组小鼠每天予东莨菪亭治疗。治疗24周后,检测碱性磷酸酶、超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽S-转移酶活性;信号蛋白及其受体,包括芳香烃受体(Aryl hydrocarbon receptor , AhR)、p53、细胞色素P450亚酶1A1(cytochrome P450 1A1 , CYP1A1)、增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)、信号转导和转录激活因子3(signal transducer and activator of transcription-3 ,Stat-3)、存活素、金属基质蛋白酶2、cyclin D1、c-myc、金属蛋白酶组织抑制因子2和半胱氨酸蛋白酶3(caspase-3) ,采用逆转录聚合酶链反应、蛋白印迹法或免疫沉淀法检测。结果:致癌物DMBA和巴豆油可以诱导毒性反应,生化指标中相关酶活性升高,AhR、CYP1A1、PCNA、Stat-3、存活素、MMP-2、cyclin D1和c-myc表达上调,p53、caspase-3和TI MP-2表达下调。荷瘤小鼠采用东莨菪亭治疗后,生化指标中相关酶的活性下降,蛋白表达和毒性生物标志物恢复正常。结论:东莨菪亭可能通过下调AhR表达来下调一些重要信号蛋白的表达,其作用机制可能是通过竞争性抑制存活素的表达。分裂原活化蛋白激酶可能也起到关键作用。东莨菪亭或许可作为化疗的替代药物用于治疗肿瘤。

关键词：东莨菪亭皮肤肿瘤 7,12-二甲基苯并蒽信号受体凋亡小鼠

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hepatitis viruses and non-Hodgkin's lymphoma: A review

引用

World Journal of Virology 2012年第6期1卷 162-173页

作者： Sibnarayan Datta soumya Chatterjee Rudragoud S Policegoudra Hemant K Gogoi Lokendra Singh Biotechnology Division Defence Research Laboratory Tezpur AssamPIN-784001 India

Non-Hodgkin's lymphoma(NHL) is among the haematological malignancies with high prevalence worldwide, causing estimated 355 900 new cases and 191 400 deaths in 2008. High prevalence of NHL is documented in economically more developed areas while low prevalence is observed in less developed areas of the globe. A wide array of environmental factors have been reported to be either directly involved or in modifying the risk of NHL development. In addition to these factors, a number of infectious agents, chiefly viruses have also been implicated in the development of NHL. This article reviews the available literature to discuss the role of hepatitis viruses in NHL development, possible mechanisms of lymphomagenesis and also identify the areas in which further research is required to better understand this disease. A brief discussion on the clinical aspects such as classification, staging, treatment approaches have also been included in this article.

关键词： Non-Hodgkin’s lymphoma Hepatitis B virus Hepatitis C virus Hepatitis G virus MiRNA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioinformatic Tools for Polyploid Crops

引用

Journal of Agricultural Science and Technology(B) 2014年第8期4卷 593-601页

作者： Fabian Grandke soumya Ranganathan Andrzej Czech Jom R. de Haan Dirk Metzler Bioinformatics Department Genetwister Technologies B. V. Wageningen 6700 Netherlands Department of Biology University of Munich Planegg-Martinsried 82512 Germany

Polyploidy is common among agriculturally important crops. Popular genetic methods and their implementations cannot always be applied to polyploid genetic data. We give an overview about available tools and their limitations in terms of levels of ploidy, auto- and allo-ploidy. The main classes of tools are genotype calling, linkage mapping and haplotyping. The usability of the tools is discussed with a focus on their applicability to data sets produced by state of the art technologies. We show that many challenges remain until the toolset for polyploidy provides similar functionalities as those which are already available for diploids. Some tools have been developed over a decade ago and are now outdated. In addition, we discuss necessary steps to overcome this shortage in the future.

关键词：工具集多倍体农作物生物信息学基因数据遗传方法基因型单倍型

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Productive Performance and Response of Green Chilli （Capsicum annum L.） to Drip Irrigation Schedules under Water Limited Conditions

引用

Journal of Agricultural Science and Technology 2010年第2期4卷 48-55页

作者： B.K. Ramachandrappa H.V. Nanjappa T.M. soumya B.N. Prabhakara Department of Agronomy Agriculture College University of Agricultural Sciences GKVK Bangalore Karnataka 560065 India

Drip irrigation is often preferred under water limited conditions over the other irrigation methods because of its high water application efficiency on account of reduced surface evaporation and percolation losses. The major limitation of drip irrigation is its initial investment. Therefore, there is a need to reduce the cost of drip installation by altering the planting geometry besides enhancing the productivity and moisture use. Results of the two years field study conducted on sandy loam soils revealed that drip irrigation at 1.0 Epan with normal row system (60 cm×30 cm) registered significantly higher yield of green chilli(14.08 t ha-1) which was on par with 0.8 Epan two rows paired system (90-45-90 cm×30 cm:13.45 t ha-1) and significantly lowest was noticed with 0.6 Epan four rows paired system(45-45-45-120 cm×30 cm ) (5.50 t ha-1). The quality parameters viz; TSS and Ascorbic acid were significantly higher with drip irrigation at 1.0 Epan with normal row system and lowest was observed with 0.6 Epan four rows paired system. Total water used was maximum in 1.0 Epan (691.7 mm) followed by 0.8 Epan irrigation (590.2 mm) and the lowest was recorded in 0.6 Epan irrigation (488.8 mm). There was a saving of water by 14.68 percent and 29.34 percent under 0.8 and 0.6 Epan drip irrigation schedules respectively over 1.0 Epan. Indications of less leaf water potential in 1.0 Epan drip irrigation (-0.58 to-0.67 MPa) followed by 0.8 Epan (-0.61 to-0.68 MPa) and more negative leaf water potential in 0.6 Epan (-0.71 to-0.88 MPa) in relation to water applied were observed. Water use efficiency recorded was maximum in 0.6 Epan normal row systems (24.1 Kg ha-mm-1) and the least was recorded in 0.6 Epan four rows paired system (11.3 Kg ha-mm-1). The significantly higher root growth characters such as root length (42.6 cm), number of primary roots (28.7), root dry weight (11.05 g plant-1) and maximum depth of penetration were recorded with 0.6 Epan four rows paired system and the

关键词： Drip irrigation greenchilli planting method water requirement leaf water potential root proliferation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

使用顺势疗法药物商陆的母酊剂从硝酸银中快速绿色合成纳米银颗粒(英文)

引用

中西医结合学报 2012年第5期10卷 546-554页

作者： soumya Sundar Bhattacharyya Jayeeta Das Sreemanti Das Asmita Samadder Durba Das Arnab De Saili Paul Anisur Rahman Khuda-Bukhsh Cytogenetics and Molecular Biology Laboratory Department of ZoologyUniversity of Kalyani

目的:研究顺势疗法药物商陆的母酊剂是否能够从硝酸银沉淀出纳米银颗粒,并通过检测这些纳米银颗粒的生物学活性确定其生物学特征。方法:将100mg硝酸银加入20mL超纯水中,并用力搅动使其混匀,再加入5mL顺势疗法药物商陆的母酊剂(商陆根的... 详细信息

目的:研究顺势疗法药物商陆的母酊剂是否能够从硝酸银沉淀出纳米银颗粒,并通过检测这些纳米银颗粒的生物学活性确定其生物学特征。方法:将100mg硝酸银加入20mL超纯水中,并用力搅动使其混匀,再加入5mL顺势疗法药物商陆的母酊剂(商陆根的乙醇提取物),再用力搅动使其混匀。300K温度下沉降迅速开始,10min后溶液中出现呈浅黄绿色的纳米银颗粒的胶质。胶质溶液经5 000×g离心后分离出纳米银颗粒。对这些纳米银颗粒进行光谱学分析、颗粒测量、电动电位测量,并使用原子力显微镜对其进行形态学观察。使用圆二色光谱分光光度法以小牛胸腺 DNA 作为靶点检测药物-DNA 相互作用和熔化温度。纳米银颗粒的生物活性以人肺癌 A549细胞株、大肠杆菌和酵母菌为实验模型进行检测。结果:顺势疗法药物商陆的母酊剂能够依据环境条件沉淀纳米银颗粒。纳米银颗粒的大小为91nm,多分散性系数为0.119,电动电位为-15.6mV。这种纳米银颗粒具有抗肿瘤和抗细菌活性,但没有显示出抗真菌活性。结论:本研究所采用的生物合成纳米银颗粒的方法无毒且具有良好的成本效益,可以作为一种有益于环境保护的新方法加以开发利用。而顺势疗法药物的母酊剂之所以具有治疗某些疾病或缓解疾病症状的作用,可能也是因为其具有纳米颗粒沉降的作用。

关键词：硝酸银金属纳米粒子十二蕊商陆顺势疗法生物合成途径

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：