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Effect of Arbuscular Mycorrhizal Fungal Inoculation on Sorghum bicolor Growth at Different Phosphate Levels: A Greenhouse Study

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Journal of Agricultural Science and Technology(B) 2019年第4期9卷 234-240页

作者： souleymane kone Fallaye Kante Ibou Diop Fatou Ndoye Abdalla Gamby Dieudhiou Aboubacry Kane Ousmane Sacko Inamoud Ibny Yattara Laboratory of Soil Microbiology (LMS) Faculty of Sciences and TechnicsUniversity of Sciences Technics and Technologies of Bamako(USTTB)BP E 3206Mali Department of Biology Vegetable Faculty of Sciences and TechnicsUniversity Cheikh Anta DiopBP 5005 Dakar-FannSenegal Common Laboratory of Microbiology Development Research InstituteSenegal Agronomic Research InstituteUniversity Cheikh Anta Diop(LCM/IRD/ISRA/UCAD)DakarSenegal

In sub-Saharan Africa, Sorghum (Sorghum bicolor) is an important cereal for both human being and animals. Unfortunately, its production is confronted to soils with deficiency of phosphorus. Traditional use of mineral phosphate on this culture fertilization is expensive and may cause contamination. It is thus necessary to seek more efficient and economic reasonable techniques to improve sorghum growth. Arbuscular mycorrhizal fungi (AMF) constitute a reference for phosphorus improvement and plant nutrition. This study aimed to investigate the effects of AMF strains (Rhizophagus irregulare, Glomus aggregatum, G. mosseae) on growth of sorghum cultivated in greenhouse on Sangalkam soil (Senegal) sterilized with or without Tilemsi natural phosphate (PNT). The phosphorus can represent until 0.2% of the dry weight of the plant. Two fertilizers were used separately and together to doses of 20 g by strain, 100 mg and 200 mg of PNT. The experiment lasted for 120 d. Results showed that mycorrhizal colonization intensity varied between 40% and 80% for all treatments. AMF inoculation increased sorghum plant height and biomass, regardless of PNT amendment. The inoculation permits to bring strain of AMF that intervene efficiently in the transportation and the availability of phosphorus for the plant.

关键词： Arbuscular mycorrhizal fungi Tilemsi natural phosphate Sorghum bicolor

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Treatment of Pelvic Limb Fractures in a Hospital of 2^e Reference in Mali

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Surgical Science 2023年第5期14卷 347-354页

作者： souleymane Diallo Sory Ibrahim Tambassi Bilal Bore Terna Traore Kalifa Coulibaly Laye Touré Ibrahima Djire Aboubacar Sidiki Sangare Salif kone Drissa Kaloga Bagayogo Aboucar Diallo souleymane kone Mamadou Konaté Soumana Traoré Cheick Oumar Sanogo Ahmadou Ibrahim Drame Abdoulaye G. Diarra Orthopedic Surgery and Traumatology Unit Tombouctou Hospital Tombouctou Mali Department of Orthopedic Surgery and Traumatology CHU BSS of Kati Kati Mali Department of Orthopedic Surgery and Traumatology Sikasso Hospital Sikasso Mali Department of Neurosurgery Mali Hospital Bamako Mali Department of Anesthesia and Resuscitation CHU Mère Enfant “Le Luxemburg” Bamako Mali Department of Surgical Hospital in Mopti Mopti Mali Department of Anesthesia and Resuscitation Mali Hospital Bamako Mali Department of General Surgery CHU of Kati Kati Mali

Introduction: A fracture is a solution in the continuity of a bone. Pelvic limb fractures may involve one or more of the bones. They constitute a real public health problem, which requires the identification of the factors inherent to this phenomenon for better prevention, but also for quality management of fractures and sequelae. Objectives: Were to describe the epidemiological, clinical and therapeutic profile of pelvic limb fractures in Timbuktu Hospital. Patients and Method: We conducted a prospective, descriptive study in the Surgery Department of Timbuktu Hospital, covering a period of one year from January 1 to December 31, 2017. We collected 39 patients who presented with fractures of the pelvic limbs, who were hospitalized and monitored throughout our study. Results: We obtained a hospital frequency of 2.86% limb fractures. Among the 39 patients included in our study, the male sex was predominant in 69.2% of cases, the average age of our patients was 20.5 years. Pupils and students were in the majority in 48.7% of cases. Road traffic accidents were the most common cause of fracture with 59.0% of cases. The tibia was the most affected segment in 38.5% of cases. Surgical treatment was predominant in 64.0% of cases. We obtained very good results in 94.87% of cases. Conclusion: Limb fractures remain frequent due to road traffic accidents. Osteosynthesis treatment provides a good result with fewer complications.

关键词： Fractures Pelvic Limb Surgical

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Detection of Oxidants Such as Hydroxyl Radicals and Chlorine Electrogenerated on a BDD Electrode by Simple Methods

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Advances in Chemical Engineering and Science 2024年第4期14卷 173-187页

作者： Konan Sylvestre Koffi Konan Martin Koffi Foffié Thiery Auguste Appia Sahi Placide Sadia Kouakou Jocelin Kimou souleymane kone Lassiné Ouattara Laboratoire de Constitution et de Raction de la Matire UFR SSMT Universit Flix Houphout Boiguy Abidjan Cte dIvoire UFR Environnement Universit Jean Lorougnon Gud Daloa Cte dIvoire

The aim of this work is to detect electrogenerated hydroxyl radicals and chlorine by simple and less expensive methods. Preparative electrolyses of perchloric acid (HClO4) and sodium chloride (NaCl) were performed on a boron-doped diamond (BDD) electrode. The hydroxyl radicals were quantified indirectly by assaying the samples from the HClO4 (0.1 M) electrolysis with a 10−4 M potassium permanganate solution. The investigations showed that the amount of hydroxyl radicals depends on the concentration of HClO4 and the current density. As for chlorine, a qualitative determination was carried out. A mixture of the electrolyte solution of HClO4 (0.1 M) + NaI (0.2 M) + 2 mL of hexane, taken in this order, leads to a purplish-pink coloration attesting to the presence of Cl2. The same test was carried out with NaBr and NaI giving pale and very pale pink colourations, respectively, showing that the intensity of the colouration depends on the strength of the oxidant present. In addition, oxidants were detected during the electrooxidation of metronidazole (MNZ). The results showed the participation of electrogenerated hydroxyl radicals. The generation of chlorine has also been proven. Furthermore, the degradation leads to a chemical oxygen demand (COD) removal rate of 83.48% and the process is diffusion-controlled.

关键词： Electrooxidation Hydroxyl Radicals Chlorine Detection

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Treatment of Pelvic Limb Fractures in a Hospital of 2^e Reference in Mali

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Surgical Science 2023年第5期

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Application of Image Compression to Multiple-Shot Pictures Using Similarity Norms With Three Level Blurring

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Computers, Materials & Continua 2019年第6期59卷 753-775页

作者： Mohammed Omari souleymane Ouled Jaafri LDDI Laboratory Mathematics and Computer Science DepartmentUniversity of AdrarAdrar 01000Algeria

be stored or transmitted in an efficient *** this work,a new idea is proposed,where we take advantage of the redundancy that appears in a group of images to be all compressed together,instead of compressing each image by *** our proposed technique,a classification process is applied,where the set of the input images are classified into groups based on existing technique like L1 and L2 norms,color *** images that belong to the same group are compressed based on dividing the images of the same group into sub-images of equal sizes and saving the references into a *** the process of extracting the different sub-images,we used the mean squared error for comparison and three blurring methods(simple,middle and majority blurring)to increase the compression *** show that varying blurring values,as well as MSE thresholds,enhanced the compression results in a group of images compared to JPEG and PNG compressors.

关键词： Image compression simple blurring middle blurring majority blurring similarity classification mean squared error

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Numerical investigation of electric field distortion induced by internal defects in composite insulators

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High Voltage 2017年第4期2卷 253-260页

作者： Gbah kone Christophe Volat Hassan Ezzaidi Modeling and Diagnostic of Power Network Equipment Laboratory University of Quebec at Chicoutimi555 Bd.de l'UniversitéQCCanada G7H 2B1

This paper presents an exhaustive study of numerical investigations based on three-dimensional finite-element method modelling of several non-ceramic insulators of 69,110 and 230 kV having different internal semi-conductive defects in terms of size and *** internal defects were positioned close to the high-voltage electrode,the ground electrode and at floating *** simulations effected on the distortion of the axial and radial E-field components close to the insulator shank between *** results obtained showed that the radial E-field component is more sensitive to the presence of an internal defect as it shows a greater distortion in presence of the *** was also observed that the E-field component distortion increases with longer internal defect and that this increase is particularly important for a defect at floating ***,it was demonstrated that the length of the insulator,for a defect of constant length,has no significant influence on the E-field component ***,the results demonstrated that the presence of a grading ring,as used on the 230 kV insulator,does not have any influence on the E-field component distortion,independently of the defect position.

关键词： distortion insulator defect

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Missing Data Analysis of the Malian National Malaria Control Program in Koulikoro

Missing Data Analysis of the Malian National Malaria Control...

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作者： souleymane TRAORE 华东师范大学

学位级别：硕士

The presence of missing data in a database is a phenomenon that every researcher is liable to meet during their work. Although the bias which can be generated by an inadequate treatment of non-response is known for a long time [27], the knowledge on the effectiveness of various methods used in the treatment of missing values is not yet fully explored. This study aims to apply the techniques used in the treatment of missing values in the calculation of the morbidity and mortality linked to Malaria in Koulikoro in Mali during the period from April to June 2005. The main problem that missing data poses is that it is impossible not to take missing values into consideration during the application of a statistical analysis method. The majority of these methods had been developed to deal with a matrix of incomplete data. In this document the statistical theories on incomplete data sets are presented and then applied on the data of Koulikoro from Malian National Malaria Control Program.

关键词： Confidence intervals imputation incomplete malaria morbidity mortality proportion

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Construction Project Management Using 5D BIM: Solution for Delay and Cost Overrun

Construction Project Management Using 5D BIM: Solution for D...

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作者： DIALLO THIERNO souleymane 绍兴文理学院

学位级别：硕士

为了成功地实现建设项目,传统项目管理的目标是进行任务协调,以在合同规定时间及预算内成功及按时交付项目。为此,必须在确定的时间范围和预算内实现某些项目目标。延迟和成本超支是两个主要缺陷,因为它们会干扰项目的成功。为了使施工...

为了成功地实现建设项目,传统项目管理的目标是进行任务协调,以在合同规定时间及预算内成功及按时交付项目。为此,必须在确定的时间范围和预算内实现某些项目目标。延迟和成本超支是两个主要缺陷,因为它们会干扰项目的成功。为了使施工过程更快、更安全、更方便和更精确,仍然存在各种挑战,因为该行业遭受质量差、通信差、成本超支和项目完成延误的困扰。建筑信息模型(BIM)的兴起是一项可选的创新,它可以解决与业务成本和时间控制相关的问题,因为它可以有效地扩大合作伙伴之间的协作。对BIM应用程序的检查表明,大多数应用程序旨在最大程度地减少建筑成本和项目时间。3D模型是几何模型,当附加时间时成为4D和附加成本时成为5D。这种方法使承包商能够可靠地确定项目成本,预期成本内的进度计划,同时允许通过考虑从模型中得出的材料数量和施工团队的生产率来优化进度计划。这项研究的目的是提供对建筑行业中BIM5D的清晰理解,基于BIM的第五维度(BIM 5D)及其应用程序,我们可以很好地描述结合了3D设计和结构的结构系统。预算和进度表为建筑项目管理提供5D工具,通过查看相应的优势、困难和动机因素有利于应用。

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Genome-wide Investigation of Germin-like Proteins and Functional Determination of SpGLP19 in Sesuvium Portulacastrum

Genome-wide Investigation of Germin-like Proteins and Functi...

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作者： Ousmane Ahmat souleymane 海南师范大学

学位级别：硕士

类萌发素蛋白(Germin-like protein,GLPs)是植物中一类胁迫响应蛋白,参与植物的多种生物与非生物胁迫应答反应过程,它与小麦(Triticum aestivum)的萌发素(germin)基因序列高度相似,是位于胞外基质的一种可溶性糖蛋白。海马齿是一种盐生... 详细信息

类萌发素蛋白(Germin-like protein,GLPs)是植物中一类胁迫响应蛋白,参与植物的多种生物与非生物胁迫应答反应过程,它与小麦(Triticum aestivum)的萌发素(germin)基因序列高度相似,是位于胞外基质的一种可溶性糖蛋白。海马齿是一种盐生植物,生长在热带沿海地区,为番杏科(Aizoaceae),海马齿属(***)的双子叶、多年沙生草本兼性盐生植物,因可以通过在细胞和组织中收集大量盐分,并在极高的盐度条件下存活,又被称为盐分收集器。为研究海马齿的耐盐机制并挖掘它的耐盐相关蛋白和基因,本研究运用多组学数据和生物信息学的分析手段对获得的海马齿GLPs基因家族进行鉴定分析,并对筛选到的SpGLP19家族成员进行了后续的转基因功能验证研究。主要研究内容及结果如下:1.在这项研究中,利用实验室前期获得的海马齿叶片响应不同浓度(0、300和600 mM)NaCl胁迫的双向电泳技术(2DE)的蛋白质组学数据,在鉴定到的96种盐响应蛋白中,筛选到了一种重要的盐响应候选蛋白,即类萌发素蛋白GLP,在2DE凝胶中的被鉴定到的该蛋白质位点为45、86、89和92。结果表明:这些蛋白位点对NaCl胁迫高度敏感,且在600 mM处理后显示显著增强的表达水平,提示可能在海马齿属植物中存在相似的GLP,在耐盐响应中起到重要作用,因此选择GLP基因进一步进行了基因家族分析,验证该蛋白在海马齿耐盐中调控的生物学功能。2.通过生物信息学的手段对海马齿的GLP家族成员进行全基因组分析鉴定,共有50个SpGLP基因被系统地鉴定,并分布在17条不同密度的染色体上,亚细胞定位预测显示这些GLP家族成员分布在细胞外。根据系统发育树分析,将海马齿、水稻和拟南芥中的GLP蛋白分为3大类(A、B和C),结果表明,这些GLP家族在功能和特征进化方面是高度多样化的。基于外显子-内含子组织分析发现,SpGLP成员具有相似的外显子-内含子结构,但内含子长度和外显子大小存在较大偏差,这为研究SpGLP成员之间的基因功能和进化关系提供了更多信息。采用多序列比对和保守基序分析研究表明SpGLP蛋白家族都具有来自萌发蛋白样蛋白家族的保守结构域。根据MEME online工具对这些保守基序的分析发现所有SpGLP成员序列中都存在cupin基序,而进化树同一分支中成员的保守基序分布更为相似,说明海马齿GLP的蛋白质具有相似的功能。通过对GLP家族的鉴定为海马齿耐盐性关键候选基因筛选提供了重要信息。另外,SpGLP基因在海马齿生长发育中可能起到不同作用,因此以海马齿不同组织和不同转录组数据以及不同盐度处理海马齿的转录组数据分析构建海马齿组织特异性和不同盐度的GLP基因表达模式,该部分数据为实验室所有,结果表明GLP基因成员在不同组织和时期中的表达水平存在一定差异,其中SpGLP26在成熟叶片中的丰度最高,SpGLP22、SpGLP37、SpGLP38、SpGLP39主要在嫩叶中表达,SpGLP49、SpGLP40、SpGLP42在幼茎中的表达水平最高。值得注意的是,SpGLP19在对照组叶片中的表达量较低,而它是在不同浓度的NaCl处理后诱导的表达水平随NaCl浓度的增加而增加,这可能表明SpGLP19在对盐的响应中起作用,后面结合生理生化和分子生物学方法对海马齿盐进行胁迫实验筛选关键候选耐盐基因进行表达量验证。3.对不同盐梯度(0、300和600 mM)处理下的海马齿进行逆境胁迫的生理指标测定和对筛选的几个候选基因进行qRT-PCR实验验证。结果显示随着海马齿植株盐度胁迫增加,对照组株高与300 mM NaCl胁迫无显著差异,600 mM NaCl胁迫时显著降低;而叶绿素和含水量先上升后下降,丙二醛含量、脯氨酸含量含量显著上升,可溶性糖含量无显著变化,表明一定盐度范围内的海马齿仍可正常生长,当超过300 mM NaCl的胁迫才开始对海马齿生长产生影响,这已经远高于模拟的海水浓度(200 mM NaCl)的生长值。此外,选取的几个候选基因的qRT-PCR的结果表明:SpGLP19、SpGLP22、SpGLP26、SpGLP38、SpGLP50、SpGLP36的荧光定量结果与转录组测序结果基本一致,证明了该测序的高可靠性,其中的SpGLP19基因表达量不同盐度胁迫后与对照相比,分别显著增加了4倍和8倍,进一步表明SpGLP19在对盐的响应中可能起到关键作用,因此以SpGLP19作为候选基因构建过表达载体进行后续的拟南芥转基因功能验证实验。4.构建了海马齿盐胁迫响应候选基因SpGLP19的植物过表达载体,以模式植物拟南芥为材料进行遗传转化实验获得转基因拟南芥种子,通过培养基抗性筛选和目的条带扩增获得阳性株的T3代种子,分别进行培养基和土培的盐胁迫

关键词：类萌发素蛋白组学分析基因家族盐胁迫响应海马齿

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Feasibility Study for The Establishment of The West African Monetary Union

Feasibility Study for The Establishment of The West African ...

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作者： DIAILO souleymane AISSATOU 东北大学

学位级别：硕士

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