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Post-Harvest Fungi Associated with Cowpea (Vigna unguiculata L. Walp.) Seeds Produced in Burkina Faso

Advances in Microbiology 2023年第3期13卷 148-163页

作者： Amidou S. Ouili Ynoussa Maiga Mahamadi Nikiema souleymane bissiri Yérobessor Dabiré Illiassou Mogmenga Cheik Omar Tidiane Compaore Aboubakar Sidiki Ouattara Laboratoire de Microbiologie et de Biotechnologies Microbiennes Universit&eacute Joseph KI-ZERBO Ouagadougou Burkina Faso Institut Supé rieur de D&eacuteveloppement Durable (ISDD) Universit&eacute de Fada N’Gourma Fada N’Gourma Burkina Faso Laboratoire de Biochimie Biotechnologie Technologie Alimentaire et Nutrition Universit&eacute Joseph KI-ZERBO Ouagadougou Burkina Faso Centre Universitaire de Banfora Universit&eacute NAZI BONI Bobo-Dioulasso Burkina Faso

Cowpea is a very popular foodstuff among people in sub-Saharan Africa. In Burkina Faso, it is the main food legume, especially in rural areas. Its production is facing difficulties including post-harvest losses caused by fungi. Therefore, the objective of this study was to isolate and identify fungal strains associated with cowpea seeds produced in Burkina Faso. Thus, a total of 108 seed samples were collected in the three agro-ecological zones of Burkina Faso. The sanitary analysis of the seeds was carried out using the direct contact method. The isolation and purification of the isolates were performed on Potato Dextrose Agar medium while their identification was done through macroscopic and microscopic phenotypical characterization using different culture media (Potato Dextrose Agar (PDA), Malt Extract Agar (MEA) and Czapeck Dox Agar (CZA)) and different identification keys. A total of 10 fungal species were isolated, with predominance of Aspergillus flavus, Aspegillus niger, Macrophomina phaseolina, Fusarium oxysporium and Rhizopus sp. whose infection rates were 70.8% to 100% of seed samples. In addition to being present in all three zones, the infection rates of Aspergillus flavus (56.55%), Aspergillus niger (20.35%) and Rhizopus (32.80%) were higher in the Sahelian zone. In the Sudano-Sahelian zone, Macrophomina (50.66%) and Fusarium (18.88%) presented the highest infection rates, while Penicillium sp. showed the highest infection rate (2.84%) in the Sudanian zone. This finding demonstrated the necessity to improve post-harvest and conservation techniques of cowpea to limit crop losses and preserve the sanitary quality of this important foodstuff.

关键词： Cowpea Seed-Borne Fungi Agro-Ecological Zones Burkina Faso

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Ants Assemblage Method According to an Age Gradient of Mango Orchards in Korhogo (Côte d’Ivoire)

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Advances in Entomology 2020年第1期8卷 56-71页

作者： Tra Bi Crolaud Sylvain Soro Senan Kouakou Lombart Yeboue Lucie Yacouba Hanna-Thérèse bissiri Yeo Kolo Konate souleymane Tano Yao Université Jean Lorougnon Guédé/UFR Agroforesterie Daloa Côte d’Ivoire Université Nangui Abrogoua UFR des Sciences de la Nature Abidjan Côte d’Ivoire Université Félix Houphouët Boigny/UFR Biosciences Abidjan Côte d’Ivoire

This study was conducted in the Korhogo region in an age gradient of mango orchards. The goal was to assess diversity and determine the influence of the environment on the composition of these organisms. Sampling was done using pitfall traps, threshing and manual collection. A total of 82 ant species were sampled on all plots. The cultivated plots are richer in ant species than the natural environment. Of the three (3) sampling methods, the Manual Collection proved to be more efficient (eff = 79.52%), followed by threshing (eff = 75.15%) and finally the least effective trap pit (eff = 73.39%). The number of individuals of the species varies according to the environment. The highest value was obtained in the young plot (485 individuals), followed by the intermediate plot (478 individuals), then the older plot (426 individuals) and finally the control plot (320 individuals). The species Camponotus acvapimensis was harvested mainly with 159 individuals. On the other hand, the species Lepisiota sp.1;Camponotus rufigenis, Camponotus sericeus and Oecophylla longinoda were specifically harvested in the control, young, intermediate and aged plots, respectively.

关键词： Ant Mango Tree Age Gradient Assemblage Diversity

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