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Mutagenic Effects of Gamma Radiation on Eight Accessions of Cowpea (Vigna unguiculata [L.] Walp.)

American Journal of Plant Sciences 2016年第2期7卷 339-351页

作者： Festus Olakunle Olasupo Christopher Olumuyiwa Ilori Brian Peter Forster souleymane bado Department of Crop Protection and Environmental Biology University of Ibadan Ibadan Nigeria Plant Breeding Section Cocoa Research Institute of Nigeria Ibadan Nigeria Biohybrids International Ltd. Reading UK Plant Breeding and Genetics Laboratory Joint FAO/IAEA Agriculture and Biotechnology Laboratory International Atomic Energy Agency Laboratories Seibersdorf Austria

Mutagenesis is used for creating new genetic variability in cultivar improvement. Optimal mutagenic treatment is required for effective mutation induction in crop species. Therefore, radio-sensitivity of cowpea accessions to gamma irradiation was investigated. Seeds of eight cowpea accessions were irradiated with 60Co gamma radiation doses of 100, 200, 300, 400 and 500 Gy. The seeds were sown in pots to evaluate the treatment effects on seed germination (SG), seedling survival (SS) and growth habits of M1 generation. Data were analyzed using descriptive statistics. Low rates of SG (10% - 45%) were recorded at higher doses (500 - 400 Gy) in Ife Brown (IB) and its derivatives, whereas high SG rates (74% - 94%) were observed in IT90K-284-2 across all treatments. Percentage SS was inversely related to gamma dosage. A wide range of LD50 for SG (329 - 1054 Gy) and SS (149 - 620 Gy) were observed across the cowpea accessions. Low LD50 scores for SG (329 - 516 Gy) and SS (149 - 357 Gy) were observed among cowpea with rough seed coat, whereas cowpea with smooth seed coat recorded higher LD50 for SG (521 and 1054 Gy) and SS (449 and 620 Gy). Seed germination LD50 and SS LD50 were highly correlated with mean coat thickness (0.899 and 0.937) than mean seed weight (0.621 and 0.678). Gamma irradiation of cowpea seed at low dosage (100 Gy) increased the vigor of M1 seedlings with respect to primary leaf area, terminal leaflet area, seedling height and plant height at six weeks. Doses of 200 Gy and above resulted in a progressive reduction in vigor of plant and seed setting of cowpea. Radio-sensitivity varied with cowpea genotype and was associated with seed testa texture, thickness and seed weight. Low gamma irradiation treatment (100 Gy) may be used to enhance seedling vigor, vegetative growth and yield of cowpea at M1 generation.

关键词： Cowpea Mutagenesis Radio-Sensitivity Gamma Irradiation Optimum Mutation Treatment

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Genetic Structure and Diversity Study of Cassava (Manihot esculenta) Germplasm for African Cassava Mosaic Disease and Fresh Storage Root Yield

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Open Journal of Genetics 2023年第1期13卷 23-47页

作者： Janatu Veronica Sesay Aiah Lebbie Richard Wadsworth Ephraim Nuwamanya souleymane bado Prince Emmanuel Norman Sierra Leone Agricultural Research Institute PMB 1313 Tower Hill Freetown Sierra Leone Department of Biological Sciences School of Environmental Sciences Njala University Njala Sierra Leone Makerere University Kampala Kampala Uganda Department of Plant Science Phytoprise GmbH DI Rudolf Schober-StraBe 4 7400 Oberwart Oberwart Austria

A better understanding of population structure and genetic diversity among cassava germplasm for African cassava mosaic disease and fresh root yield traits is useful for cassava improvement programme. Phenotype-based selection for these traits is cumbersome due to phenotypic plasticity and difficulty in screening of phenotypic-induced variations. This study assessed quantitative trait loci (QTL) regions associated with African cassava mosaic disease (ACMD) and fresh storage root yield (FSRY) in 131 cassava (Manihot esculenta) genotypes using a genome-wide association study (GWAS). The single nucleotide polymorphism (SNP) loci and associated candidate genes, when validated, would be a valuable resource for marker-assisted selection in the breeding process for development of new cassava genotypes with improved resistance to ACMD and desirable high root yield. Population structure analysis using 12,500 SNPs differentiated the 131 genotypes into five distinct sub-groups (K = 5). Marker-trait association (MTA) analysis using the generalized linear model identified two QTL regions significant for ACMD and three for FSRY. This study demonstrated that DArTseq markers are useful genomic resources for genome-wide association studies of ACMD and FSRY traits in cassava for the acceleration of varietal development and release.

关键词： Population Structure Genetic Variability GWAS SNP Markers Cassava

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Micro-Tuber Production in Diploid and Tetraploid Potato after Gamma Irradiation of in Vitro Cuttings for Mutation Induction

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American Journal of Plant Sciences 2016年第14期7卷 1871-1887页

作者： souleymane bado Margit Laimer Ndiogou Gueye Ndeye Fatou Deme Enoch Sapey Abdelbagi Mukhtar Ali Ghanim Vivian Carol Blok Brian Peter Forster Plant Breeding and Genetics Laboratory (PBGL) Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture FAO/IAEA Laboratories Seibersdorf Austria Plant Biotechnology Unit Department of Biotechnology University of Natural Resources and Life Sciences Vienna Austria Biotechnology Laboratory of Mushrooms Department of Plant Biology Faculty of Sciences & Techniques University of Cheikh Anta Diop Dakar Fann Sénégal Council for Scientific and Industrial Research (CSIR)-Oil Palm Research Institute (OPRI) Kade Ghana The James Hutton Institute Errol Rd Invergowrie Dundee UK BioHybrids International Ltd. Reading UK

Micro-tubers are important propagules in potato breeding and potato production, and they are also dormant and easily transported and therefore good targets for mutation induction in potato mutation breeding. A prerequisite for mutation breeding is to determine optimal mutation treatments. Therefore, radio-sensitivity tests of a tetraploid and a diploid potato to gamma irradiation were undertaken. Effects of different gamma sources on radio-activity were also studied. In vitro potato cuttings were gamma irradiated using a wide dose range (0, 3, 6, 9, 12, 15 and 20 Gy). The irradiated cuttings were then cultured to induce micro-tubers directly in vitro. Micro-tuber morphotypes were assessed after irradiation of cuttings using three gamma sources with emission activities of 1.8, 7.07 and 139 Gy/min. The diploid species (Solanum verrucosum) was more radio-sensitive than the tetraploid cultivar Desirée (Solanum tuberosum). Gamma dose rates had significant influences on subsequent micro-tuber production at various mutant generations. Effects included reductions in the number, size and weight of micro-tubers produced. Gamma dose was more lethal for the diploid potato genotype and micro-tubers produced were small compared to those produced by the tetraploid genotype after irradiation. Different treatments are recommended for diploid and tetraploid potato irradiation in producing large mutant micro-tuber populations. The mutant micro-tuber populations may then be screened for interesting mutations/trait for both genetics and plant breeding purposes.

关键词： Potato in Vitro Cuttings Micro-Tubers Mutation Induction Gamma Irradiation Mutant Populations Recommended Dose Rates

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Prediction of Salt Tolerance in Rice （Oryza Sativa） Based on Shoot Ion Content under Non-Stressed Conditions

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材料科学与工程（中英文A版） 2016年第1期6卷 1-16页

作者： souleymane bado Brian Peter Forster Roman Padilla-Alvarez Christian Resch Alessandro Migliori Yacouba Diawara Milko Jaksic Abdelbagi Mukhtar Ali Ghanim Stephen Nielen Margit Laimer Plant Breeding and Genetics Laboratory (PBGL) Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture IAEA Laboratories Seibersdorf International Atomic Energy Agency PO Box 100 Wagramerstrasse 5 A-1400 Vienna Austria Current address: BioHybrids International Ltd P.O. Box 2411 Earley Reading RG6 5FY United Kingdom Physics Chemistry and Instrumentation Laboratory IAEA Laboratories Seibersdorf International Atomic Energy Agency POBox 100 Wagramerstrasse 5 A-1400 Vienna Austria Soil and Water Management and Crop Nutrition Laboratory (SWMCNL) Joint FAO/IAEA Division of Nuclear Techniques inFood and Agriculture IAEA Laboratories Seibersdorf International Atomic Energy Agency PO Box 100 Wagramerstrasse 5A-1400 Vienna Austria Institut Ruder Boskovic Bijenicka cesta 54 10000 Zagreb Croatia Plant Biotechnology Unit Department of Biotechnology University of Natural Resources and Applied Life Sciences Muthgasse 18 A-1190 Vienna Austria

Salt is a major abiotic stress threatening crop plants such as rice. Tolerance to salt is complex and regulated by numerous genetic and non-genetic factors. To date most screens for salt tolerance rely on plant performance in stressed conditions. This article describes the ability to screen for salt tolerance in non-stressed conditions. The work is based on correlations between 62 rice genotypes in salt stress and non-stress conditions and measuring the intake of elements using Particle Induced X-ray Emission and X-ray Fluorescence. Roots and shoots were analysed though shoots provided easier and more robust materials to work with. Data were interpreted using multivariate statistical analysis which showed the intake ratio of elements across tolerant, moderately tolerant and susceptible rice genotypes. Tolerant genotypes exhibited a larger intake of elements, and a classification criteria based on Canonical Discriminant Analysis allowed differentiation of salt tolerant genotypes not only under salt stress but, significantly, also under non-stressed conditions. Thus, shoot element content in benign conditions can be used as an indicator for salt tolerance. These studies suggest that many salt tolerance mechanisms in rice cultivars are constitutive, they are not switched on by stress, and this has implications for physiological and genetic studies, especially in crop plants. The approach has practical application as it allows pre-screening in non-stressed conditions, from which candidate salt tolerant genotypes may be selected for subsequent testing in saline field conditions, selecting in benign conditions provides greater seed harvest of the next generation which can be used in multiple tests.

关键词：水稻基因型耐盐性 Oryza 离子含量应激非遗传因素多变量统计分析元素含量

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Contribution of Satellite Observations in the Optical and Microphysical Characterization of Aerosols in Burkina Faso, West Africa

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Atmospheric and Climate Sciences 2024年第1期14卷 154-171页

作者： Nébon bado Serge Dimitri Bazyomo Germain Wende Pouiré Ouedraogo Bruno Korgo Mamadou Simina Dramé Florent P. Kieno Sié Kam Laboratoire d’Energies Thermiques Renouvelables (L.E.T.RE) Université Joseph KI ZERBO Ouaga Burkina Faso Laboratoire de Physique de l’Atmosphère et de l’Océan Siméon-Fongang Université Cheikh Anta DIOP Dakar-Fann Sénégal Institute of Mathematics and Physical Sciences University of Abomey-Calavi Porto-Novo Benin

In this work, we proceed to an optical and microphysical analysis of the observations reversed by the MODIS, SeaWiFS, MISR and OMI sensors with the aim of proposing the best-adapted airborne sensor for better monitoring of aerosols in Burkina Faso. To this end, a comparison of AOD between satellite observations and in situ measurements at the Ouagadougou site reveals an underestimation of AERONET AOD except for OMI which overestimates them. Also, an inter-comparison done based on the linear regression line representation shows the correlation between the aerosol models incorporated in the airborne sensor inversion algorithms and the aerosol population probed. This can be seen through the correlation coefficients R which are 0.84, 0.64, 0.55 and 0.054 for MODIS, SeaWiFS, MISR and OMI respectively. Furthermore, an optical analysis of aerosols in Burkina Faso by the MODIS sensor from 2001 to 2016 indicates a large spatial and temporal variability of particles strongly dominated by desert dust. This is corroborated by the annual and seasonal cycles of the AOD at 550 nm and the Angström coefficient measured in the spectral range between 412 nm and 470 nm. A zoom on a few sites chosen according to the three climatic zones confirms the majority presence of mineral aerosols in Burkina Faso, whose maxima are observed in spring and summer.

关键词： AERONET Airborne Sensors Aerosol Optical and Microphysical Properties

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Genetic Structure and Diversity Study of Cassava (Manihot esculenta ) Germplasm for African Cassava Mosaic Disease and Fresh Storage Root Yield

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Open Journal of Genetics（遗传学期刊） 2023年第1期

作者： Janatu Veronica Sesay Aiah Lebbie Richard Wadsworth Ephraim Nuwamanya souleymane bado Prince Emmanuel Norman

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Mother-to-Child Transmission of Human Papillomavirus in Burkina Faso

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American Journal of Molecular Biology 2024年第1期14卷 13-24页

作者： Koudpoko Madeleine Kabre Djénéba Ouermi Théodora Mahoukèdè Zohoncon Fatié Porzé Wilfried Traore Prosper bado Abdoul Karim Ouattara Ouamini Pulchérie De Prisca Gnoumou Rogomenoma Alice Ouedraogo Albert Théophane Yonli Punya Akouélé Kuassi-Kpede Charlemagne Marie Ragnag-Néwendé Ouedraogo Jacques Simpore Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Faculté des Sciences de la Santé Université Saint Thomas d’Aquin Ouagadougou Burkina Faso Laboratoire de Biologie Moléculaire H&#244pital Saint Camille de Ouagadougou (HOSCO) Ouagadougou Burkina Faso University Joseph Ki-Zerbo UFR/SDS Ouagadougou Burkina Faso

Introduction: Human papillomavirus (HPV) infection is the most widespread sexually transmitted infection in the world. Today, there is growing evidence that HPV can be transmitted early in life, and one potential route is mother-to-child transmission. Data on this route of HPV transmission are scarce in Africa and particularly in Burkina Faso, where no data on the subject are yet available. The aim of our study was to estimate the rate of mother-to-child transmission of HPV infection and to identify circulating genotypes. Methodology: Cervico-uterine samples were collected from 100 full-term pregnant women and, buccal samples were obtained from their newborns at Hopital Saint Camille de Ouagadougou (HOSCO) by the specialist physician. HPV DNA amplification and genotyping were performed by PCR followed by hybridization using the HPV Direct Flow Chips kit, detecting 36 genotypes including 18 high-risk and 18 low-risk. Results: The prevalence of HPV in newborns was 8% (8/100). Six (6) HPV-positive neonates had HPV-positive mothers, while 2 HPV-positive neonates had HPV-negative mothers. The vertical transmission rate was 26.09% (6/23). Mother-newborn genotypes were concordant. However, the genotype profile of the newborns was more restricted than that of the mothers. Conclusion: HPV DNA was found in 8% of newborns in our study. The genotype profile of the mother-newborn pair was concordant. Asymptomatic HPV infection in a pregnant woman could constitute a risk factor for vertical transmission.

关键词： Mother-to-Child Transmission Human Papillomavirus PCR Genotypes Burkina Faso

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Molecular Diagnosis of Sexual Differentiation Disorders in Burkina Faso

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Journal of Biosciences and Medicines 2024年第3期12卷 1-15页

作者： Bazie Bapio Valérie Elvira Jean Télesphore Sawadogo Soumaïla Zohoncon Théodora Mahoukèdè Tall Ahmadou bado Prosper Soubeiga Serge Théophile Ouattara Moussa Sorgho Abel Kiendrebeogo Touwendpoulimdé Isabelle Yonli Albert Theophane Djigma Florencia Millogo/Traore Timongo Françoise Danielle Simpore Jacques Centre National de la Recherche Scientifique et Technologique (CNRST) Institut de Recherche en Sciences de la Santé (IRSS) Ouagadougou Burkina Faso Université Joseph KI-ZERBO Unité de Formation en Sciences de la Vie et de la Terre Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigonie (CERBA) Ouagadougou Burkina Faso Centre Hospitalier Universitaire Régional de Ouahigouya Ouagadougou Burkina Faso Centre Hospitalier Universitaire Yalgado Ouedraogo Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Sex development anomalies represent a group of congenital pathologies in which chromosomal, gonadal and anatomical sex differentiation is atypical. The aim of our study was to use molecular biology techniques to elucidate sex in cases of anomalies of sexual differentiation in Burkina Faso. This cross-sectional study took place from March 2023 to June 2023. Oral and blood samples were collected respectively using sterile swabs and stored on Swab Kits and NUCLEIcardTM (https://***/product-ranges/nucleic-card/) were used to determine gonosome profiles. Extraction was carried out using the DNA Swap solution Kit and the DNA IQ System Kit, and a PowerPlex? 21 kit (Promega) for amplification. The Applied Biosystems 9700 thermal cycler was used for PCR followed by 36 cm capillary electrophoresis in the Applied Biosystems 3130 prism sequencer. Sequence files were analyzed using GeneMapper IDX v. 3.2 software. Seven (07) patients were registered during the study period. There were 4 cases of XX DSD or 46, XX DSD and 3 cases of XY DSD or 46, XY DSD. The median age of our patients was 16 years. Civilian sex was male in 4 cases and female in 3. The most frequent reason for consultation was micropenis in 3 cases, followed by primary amenorrhea and sex ambiguity. There were 03 cases of discrepancy between genetic sex and civil sex. The accessibility of molecular diagnosis is little known to clinicians. XX DSDs or 46, XX DSDs were the most frequent (4/7) in our study. The problem facing this situation is early diagnosis to help prevent complications in Burkina Faso.

关键词： Gonosome DSD Abnormal Sexual Development Burkina Faso

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HPV/HBV or HPV/HCV Co-Infections in Women Treated for Chronic Hepatitis at Hôpital Saint Camille in Ouagadougou, Burkina Faso

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American Journal of Molecular Biology 2024年第1期14卷 1-12页

作者： Estelle Ouédraogo Théodora Mahoukèdè Zohoncon Bagora Bayala Prosper bado Rose P. Clémence Da Rogomenoma Alice Ouedraogo Ina Marie Angèle Traoré Punya Akouélé Kuassi-Kpede Samiratou Ouédraogo Essi Etonam Dovo Lassina Traoré Albert Théophane Yonli Florencia Wendkuuni Djigma Olga Mélanie Lompo Jacques Simpore Laboratory of Molecular Biology and Genetics (LABIOGENE) University Joseph KI-ZERBO Ouagadougou Burkina Faso Centre of Biomolecular Research Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Hô spital Saint Camille of Ouagadougou (HOSCO) Ouagadougou Burkina Faso Faculty of Health University Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Ecole Normale Supérieure Koudougou Burkina Faso Chair “Research and Action Against Cancer” Département de Santé Publique Training and Research Unit in Health Sciences University Joseph KI-ZERBO Ouagadougou Burkina Faso Observatoire National de la Santé de la Population Institut National de Santé Publique Ouagadougou Burkina Faso The Department of Epidemiology Biostatistics and Occupational Health School of Population and Global Health Montreal Canada Laboratory of Pathological Anatomy and Cytology University Joseph KI-ZERBO Ouagadougou Burkina Faso

Introduction: Cervical cancer is a public health concern and is mainly caused by Human papillomaviruses (HPV). In many parts of the world, studies are being carried out to understand the different genotypes to better tackle this issue. We conducted a study to determine the prevalence of HPV genotypes in women with chronic hepatitis B or C infection, co-infected or not with HIV, treated at the Hôpital Saint Camille in Ouagadougou (Burkina Faso). Methods: This study was conducted from April to July 2023, including 100 women in gastroenterology at Hôpital Saint Camille. A questionnaire on their socio-demographic and life style was administrated;and endocervical samples were collected using sterile swabs and then sent to Centre of Biomolecular Research Pietro Annigoni (CERBA). HPV molecular detection and genotyping were performed by PCR and hybridization using the HPV Direct Flow Chips kit. Data were analysis using chi square test or Fischer’s exact test with a significance threshold for p Results: The prevalence of HPV infection was 28% (28/100) on the sample of women tested. The most frequent genotypes were HPV 52 (8.33%), followed by HPV 18 and 68 (6.25% each) for high-risk HPVs, and HPV 6, 44/55 and 62/81 (8.33% each) for low-risk HPVs. Conclusion: This study, the first of its kind in Burkina Faso on this group of the population, reveals that the most frequent genotypes found in this study are not included in the vaccine available in Burkina Faso (Gardasil®4).

关键词： Human Papillomavirus Cervical Cancer Hepatitis Human Immunodeficiency Virus Burkina Faso

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Distribution of High-Risk Human Papillomavirus Genotypes in Precancerous Cervical Lesions in Ouagadougou, Burkina Faso

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Open Journal of Obstetrics and Gynecology 2016年第4期6卷 196-204页

作者： Charlemagne Ouédraogo Théodora Mahoukèdè Zohoncon Esther M. A. Traoré souleymane Ouattara Prosper bado Clarisse T. Ouedraogo Florencia W. Djigma Djénéba Ouermi Dorcas Obiri-Yeboah Olga Lompo Simon A. Akpona Jacques Simpore Pietro Annigoni Biomolecular Research Center (CERBA) Ouagadougou Burkina Faso UFR/SDS University of Ouagadougou Ouagadougou Burkina Faso Laboratory of Molecular Biology and Genetics (LABIOGENE) University of Ouagadougou Ouagadougo Burkina Faso Teaching and Research Unit in Biochemistry and Molecular Biology Faculty of Medicine University of Parakou Parakou Republic of Benin Faculty of Medicine Université Saint Thomas d’Aquin Ouagadougou Burkina Faso University of Cape Coast School of Medical Sciences Microbiology Department University Post Office Cape Coast Ghana Yalgado Ouédraogo University Hospital (CHU/YO) Ouagadougou Burkina Faso

Aims: We aimed at identifying the high-risk HPV genotypes associated with high-grade dysplastic cervical lesions in Burkina Faso. The available vaccines to Burkina Faso only protect against two high risk HPV genotypes: HPV 16 and 18. Are the genotypes identified in the high-grade precan-cerous lesions in this survey covered by the available vaccines? Methods: The detection and genotyping of high-risk HPV have been conducted based on 118 formalin-fixed and paraffin-embedded archived tissues using the “HPV Genotypes 14 Real-TM Quant” (Sacace biotechnologies®, Italy) kit allowing for the detection of fourteen high-risk HPV genotypes: HPV 16, 31, 18, 39, 45, 59, 33, 35, 56, 68, 51, 52, 58 and 66. Results: The prevalence of high-risk HPV infections was 48.8% based on the appropriate PCR results (21/43). The most common HPV genotypes were HPV 39 (21.7%), HPV 35 (13.0%) and HPV 45 (13.0%). Two cases of multiple infections between HPV 39 - 45 and HPV 39 - 59 have been observed. HPV 16 was not detected in this study. Conclusions: We noted a high prevalence rate for HPV 39, HPV 35 and HPV 45, which are not covered by the commercial vaccines. We also found that the prevalence of HPV 18 was very low in this study and HPV 16 was not detected.

关键词： HPV Cancer CIN Cervix Burkina Faso

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