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Magnetic resonance evaluation of human mesenchymal stem cells in corpus cavernosa of rats and rabbits

Asian Journal of Andrology 2007年第3期9卷 361-367页

作者： Yun-Seob Song ja-Hyeon Ku Eun-Seop Song Jung-Hoon kim Jin-Suck Jeon Kong-Hee Lee sook-ja kim Hee-Jeong Cheong Ik-Sung Lim Dongho Choi Jong-Ho Won Department of Urology Stem Cell therapy Center Soonchunhyang School of Medicine Seoul 140-743 Korea Department of Urology Seoul National University Hospital Seoul 110-744 Korea Department of Obstetrics and Gynecology Inha University School of Medicine Incheon 400-103 Korea Department of Radiology Soonchunhyang School of Medicine Seoul 140-743 Korea Department of Internal Medicine Soonchunhyang School of Medicine Seoul 140-743 Korea Department of Urology Soonchunhyang School of Medicine Seoul 140-743 Korea Stem Cell therapy Center Soonchunhyang School of Medicine Seoul 140-743 Korea Department of Industrial Management ＆ Engineering Namseoul University College of Engineering Cheonan 330-707 Korea Department of Surgery Stem Cell therapy Center Soonchunhyang School of Medicine Seoul 140-743 Korea Department of Internal Medicine Stem Cell therapy Center Soonchunhyang School of Medicine Seoul 140-743 Korea

Aim： To investigate whether the biological process of superparamagnetic iron oxide （SPIO）-labeled human mesenchymal stem cells （hMSCs） may be monitored non-invasively by using in vivo magnetic resonance （MR） imaging with conventional 1.5-T system examinations in corpus cavernosa of rats and rabbits. Methods： The labeling efficiency and viability of SP10-labeled hMSCs were examined with Prussian blue and Tripan blue, respectively. After SPIO-labeled hMSCs were transplanted to the corpus cavernosa of rats and rabbits, serial T2-weighted MR images were taken and histological examinations were carried out over a 4-week period. Results： hMSCs loaded with SPIO compared to unlabeled cells had a similar viability. For SPIO-labeled hMSCs more than lx 105 concentration in vitro, MR images showed a decrease in signal intensity. MR signal intensity at the areas of SPIO-labeled hMSCs in the rat and rabbit corpus cavernosa decreased and was confined locally. After injection of SPIO-labeled hMSCs into the corpus cavernosum, MR imaging demonstrated that hMSCs could be seen for at least 12 weeks after injection. The presence of iron was confirmed with Prussian blue staining in histological sections. Conclusion： SP10-labeled hMSCs in corpus cavernosa of rats and rabbits can be evaluated non-invasively by molecular MR imaging. Our findings suggest that MR imaging has the ability to test the long-term therapeutic potential of hMSCs in animals in the setting of erectile dysfunction.

关键词： human mesenchymal stem cells magnetic resonance stem cells penis cell labeling corpus cavemosa

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Operational Implementation of the ATOVS Processing Procedure in KMA and Its Validation

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Advances in Atmospheric Sciences 2003年第3期20卷 398-414页

作者： Myoung-Hwan AHN Mee-ja kim Chu-Yong CHUNG Ae-sook SUH RemoteSensing Research Laboratory/Meteorological Research Institute RemoteSensing Research Laboratory/Meteorological Research InstituteRemoteSensing Research Laboratory/Meteorological Research InstituteRemoteSensing Research Laboratory/Meteorological Research Institute Seoul 156-720KoreaSeoul 156-720KoreaSeoul 156-720KoreaSeoul 156-720Korea

The Korea Meteorological Administration (KMA) has processed the data from the advanced TOVS (ATOVS) onboard NOAA-16 satellite since May 2001. The operational production utilizes the AAPP (ATOVS and AVHRR Processing Package) of EUMETSAT and IAPP (International ATOVS Processing Package) of the University of Wisconsin. For the initial guess profiles, the predicted fields (usually 6 to 12 hour forecasted fields) from the global aviation model of NOAA/NCEP are used. The average number of profiles retrieved from the ATOVS data is about 1,300 for each morning and afternoon orbit at about 18 and 06 UTC, respectively. The retrieved temperature and dew point temperatures are provided to forecasters in real time and used for initialization of prediction models. With the advanced microwave sensor (AMSU; Advanced Microwave Sounding Unit), accuracy of the ATOVS products is expected to be better than that of the TOVS products, especially in cloudy conditions. Indeed, the preliminary results from a validation study with the collocated radiosonde data during a 8-month period, from May to December 2001, for the East Asia region show an improved accuracy of the ATOVS products for cloudy skies versus the TOVS, especially for higher altitudes. The RMS (Root Mean Square) difference between the ATOVS products and radiosonde data is about 1.3°C for both clear and cloudy conditions, except for near the ground and at higher altitudes, at around 200 hPa. There is no significant temporal variation of the error statistics at all pressure levels. In case of the water vapor mixing ratio, the largest difference is shown at lower altitudes, while the accuracy is much better for the clear sky cases than the cloudy sky cases. The bias and RMSE at lower altitudes is about 0.557 g kg−1 and 2.5 g kg−1 and decrease significantly with increasing altitude.

关键词： ATOVS Processing and validation temperature and humidity profiles

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Species delimitation in the green algal genus Codium (Bryopsidales) from Korea using DNA barcoding

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Acta Oceanologica Sinica 2015年第4期34卷 114-124页

作者： LEE Hyung Woo kim Myung sook Department of Biology Jeju National University

Codium, one of the largest marine green algal genera, is difficult to delimit species boundary accurately based on morphological identification only. DNA barcoding is a powerful tool for discriminating species of seaweeds. The plastid elongation factor TU （tufA） is considered as maker to perform DNA barcoding of green algal species than rbcL gene due to universality and rapid evolution rate. We conducted DNA barcoding application to Codium specimens from the Jeju Island, Korea to overcome the limit of morphological identification and to confirm the species diversity. As a result of applying tufA marker, we newly generated fifty-five tufA barcodes to resolve eight species. TufA marker exhibited 6.1%–21.8% interspecific divergences, wider than the gap of rbcL exon 1, 3.5%–11.5%. Molecular analysis of rbcL exon 1 sequences of Codium revealed eight distinct species like tufA analysis separated in five phylogenetic groups. DNA barcoding of the genus Codium using tufA marker is more helpful to overcome the limit of morphological identification, and this is more potential to reveal cryptic species and to resolve the relationships among subspecies than rbcL analysis alone. The complement of tufA barcoding and rbcL analyses including morphology for the genus Codium in the northwestern Pacific will give much more reliable achievement for discovering species diversity and resolving the phylogenetic relationships.

关键词： Codium DNA barcoding rbcL Species delimitation tufA

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Stability of Symmetric Solitary Wave Solutions of a Forced Korteweg-de Vries Equation and the Polynomial Chaos

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Advances in Applied Mathematics and Mechanics 2012年第6期4卷 833-847页

作者： Hongjoong kim Kyoung-sook Moon Department of Mathematics Korea UniversitySeoul 136-701Korea Mathematics&Information Gachon UniversityGyeonggi-do 461-701Korea

In this paper,we consider the numerical stability of gravity-capillary waves generated by a localized pressure in water of finite depth based on the forced Korteweg-de Vries(FKdV)framework and the polynomial *** stability studies are focused on the symmetric solitary wave for the subcritical flow with the Bond number greater than one *** its steady symmetric solitarywave-like solutions are randomly perturbed,the evolutions of some waves show stability in time regardless of the randomness while other waves produce unstable *** representing the perturbation with a random variable,the governing FKdV equation is interpreted as a stochastic *** polynomial chaos expansion of the random solution has been used for the study of stability in two *** it allows us to identify the stable solution of the stochastic governing *** it is used to construct upper and lower bounding surfaces for unstable solutions,which encompass the fluctuations of waves.

关键词： Stability solitary waves polynomial chaos forced Korteweg-de Vries equation

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A novel isoxazole compound CM2-II-173 inhibits the invasivephenotype of triple-negative breast cancer cells

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Oncology Research 2023年第6期31卷 867-875页

作者： EUN sook kim SANGHEE kim AREE MOON Duksung Innovative Drug Center College of PharmacyDuksung Women’s UniversitySeoul03169Korea College of Pharmacy Seoul National UniversitySeoul08826Korea

Invasion and metastasis are important hallmarks of breast cancer and are the leading cause of patient ***-negative breast cancer (TNBC) is an aggressive type of breast cancer characterized by a poor prognosis and a lackof effective targeted therapies. The present study investigated the inhibitory effect of a novel FTY720 derivative on theinvasive phenotype of TNBC cells. Here, we showed that a novel compound with an isoxazole ring, 4-(3-Decylisoxazol-5-yl)-1-hydroxy-2-(hydroxymethyl)butan-2-aminium chloride (CM2-II-173), significantly inhibitedinvasiveness of MDA-MB-231 TNBC cells. Expression of matrix metalloproteinase (MMP)-9 and invasiveness ofMCF10A normal breast cells induced by sphingosine-1-phosphate (S1P) were reduced by CM2-II-173 *** of pMEK1, pAkt, pERK, and p38 MAPK by S1P were inhibited by treatment with *** and anchorage-independent growth of MDA-MB-231 TNBC cells were significantly decreased by CM2-II-173. CM2-II-173 efficiently induced apoptosis in MDA-MB-231 TNBC cells. CM2-II-173 significantly inhibitedinvasive phenotypes of breast, liver, prostate, and ovarian cancer cells. CM2-II-173 exhibited a more potent effect onthe invasiveness of MDA-MB-231 TNBC cells compared to FTY720. Taken together, this study demonstrated thatCM2-II-173 has the potential to be a lead compound that can inhibit cancer progression of not only TNBC cells, butalso of liver, prostate, and ovarian cancer cells.

关键词： MMP-9 Cell invasion Breast cells

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Cloning,expression in Escherichia coli,and enzymatic properties of laccase from Aeromonas hydrophila WL-11

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Journal of Environmental Sciences 2010年第4期22卷 635-640页

作者： Jing Wu Kyoung-sook kim jai-Heon Lee Young-Choon Lee Department of Biotechnology Dong-A University Busan 604-714 South Korea. Brain Korea 21 Center for Silver-Bio Industrialization Dong-A University Busan 604-714 South Korea

A strain WL-11 with high laccase activity was isolated from activated sludge collected from the effluent treatment plant of a textile and dyeing industry. It was identified as Aeromonas hydrophila by physiological test and 16S rDNA sequence analysis. A gene encoding of laccase from a newly isolated Aeromonas hydrophila WL-11 was cloned and characterized. Nucleotide sequence analysis showed an open reading frame of 1605 bp encoding a polypeptide comprised of 534 amino acids. The primary structure of the enzyme predicted the structural features characteristic of other laccases, including the conserved regions of four histidine-rich copper-binding sites. The predicted amino acid sequence showed a high homology （more than 60%） with bacterial laccases in the genome and protein databases and the highest degree of similarity （61% identity） was observed with the multicopper oxidase of KlebsieUa sp. 601. When expressed in Escherichia coli, the recombinant enzyme was overproduced in the cytoplasm as soluble and active form. The purified enzyme had an optimum pH of 2.6 and 8.0 for ABTS （2,2＇-azino-bis（3-ethylbenzthiazolinesulfonic acid） and DMP （2,6-dimethoxyphenol）, respectively. The kinetic study on ABTS revealed a higher affinity of this enzyme to this substrate than DMP.

关键词： cloning expression enzymatic properties laccase Aeromonas hydrophila

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Nuclear Planetology:Especially Concerning the Moon and Mars

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Research in Astronomy and Astrophysics 2012年第10期12卷 1313-1380页

作者： Kyeong ja kim Nobuyuki Hasebe Korea Institute of Geoscience and Mineral Resources Daejeon305-350 Korea Research Institute for Science and Engineering Waseda UniversityShinjukuTokyo 169-8555 Japan

To approach basic scientific questions on the origin and evolution of plan- etary bodies such as planets, their satellites and asteroids, one needs data on their chemical composition. The measurements of gamma-rays, X-rays and neutrons emit- ted from their surface materials provide information on abundances of major elements and naturally radioactive gamma-ray emitters. Neutron spectroscopy can provide sen- sitive maps of hydrogen- and carbon-containing compounds, even if buried, and can uniquely identify layers of carbon-dioxide frost. Nuclear spectroscopy, as a means of compositional analysis, has been applied via orbital and lander spacecraft to extrater- restrial planetary bodies： the Moon, Venus, Mars, Mercury and asteroids. The knowl- edge of their chemical abundances, especially concerning the Moon and Mars, has greatly increased in recent years. This paper describes the principle of nuclear spec- troscopy, nuclear planetary instruments carried on planetary missions so far, and the nature of observational results and findings of the Moon and Mars, recently obtained by nuclear spectroscopy.

关键词： nuclear planetology: planetary remote sensing -- elemental mapping --nuclear science payloads -- chemical abundance

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Iksan526 Rice Callus Extract Induces Dedifferentiation of Rabbit Articular Chondrocytes via ERK1/2 and PI-3K/Akt Pathways

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Rice science 2020年第6期27卷 504-514页

作者： Seong-Hui EO Song ja kim Department of Biological Sciences College of Natural SciencesKongju National UniversityGongju 32588Republic of Korea

The resveratrol-enriched transgenic rice line Iksan526(IS526),first developed by the Rural Development Administration of Korea using genetic engineering techniques,shows beneficial health effects in mitigating metabolic syndrome and ***,the effects of IS526 on the differentiation of chondrocytes and the underlying mechanism have not been investigated in *** this study,the effects and cellular regulatory mechanisms of IS526 on rabbit articular chondrocytes were *** IS526 callus extract treatment,the expression levels of differentiation-related proteins were detected via western blotting,Alcian blue staining and immune-luorescence ***526 decreased the type Ⅱ collagen and proteoglycan levels in dose-and time-dependent *** further analyzed the effects of IS526 on skeleton genesis in zebrafish larvae using Alcian blue staining,which showed a reduction in cartilage formation along with increased production of matrix metalloproteinase(MMP)-***526 also increased the phosphorylation of ERK1/2 and p38 kinase but inhibited the phosphorylation of *** inhibition of MMP-13 blocked the IS526-induced decrease in type Ⅱ collagen *** of p38 kinase or PI-3K/Akt with SB203580 and LY294002 enhanced the suppression of type Ⅱ collagen,but the blockage of ERK-1/2 by PD98059 rescued IS526-induced *** results suggested that IS526 regulates type Ⅱ collagen and MMP-13 expression via the ERK1/2 and PI-3K/Akt pathways in rabbit articular chondrocytes.

关键词： rice chondrocyte typeⅡcollagen matrix metalloproteinase-13 rice callus extract

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Glucose-responsive artificial promoter-mediated insulin gene transfer improves glucose control in diabetic mice

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World Journal of Gastroenterology 2012年第44期18卷 6420-6426页

作者： jaeseok Han Eung-Hwi kim Woohyuk Choi Hee-sook Jun Department of Microbiology and Infectious Diseases University of Calgary3330 Hospital Drive N.W.CalgaryAlberta T2N 4N1Canada Del E.Webb Neuroscience Aging and Stem Cell Research CenterSanford Burnham Medical Research InstituteLa JollaCA 92037United States Lee Gil Ya Cancer and Diabetes Institute Gachon University7-45 SondodongYeonsu-kuIncheon 406-840South Korea College of Pharmacy Gachon University7-45 Sondo-dongYeonsu-kuIncheon 406-840South Korea

AIM:To investigate the effect of insulin gene therapy using a glucose-responsive synthetic promoter in type 2 diabetic obese ***:We employed a recently developed novel insulin gene therapy strategy using a synthetic promoter that regulates insulin gene expression in the liver in response to blood glucose level *** intravenously administered a recombinant adenovirus expressing furin-cleavable rat insulin under the control of the synthetic promoter(rAd-SP-rINSfur) into diabetic Lepr db/db mice.A recombinant adenovirus expressing β-galactosidase under the cytomegalovirus promoter was used as a control(rAd-CMV-βgal).Blood glucose levels and body weights were monitored for 50 *** and insulin tolerance tests were *** staining was performed to investigate islet morphology and insulin ***:Administration of rAd-SP-rINSfur lowered blood glucose levels and normoglycemia was maintained for 50 d,whereas the rAd-CMV-βgal control virus-injected mice remained *** tolerance tests showed that rAd-SP-rINSfur-treated mice cleared exogenous glucose from the blood more efficiently than control virus-injected mice at 4 wk [area under the curve(AUC):21 508.80 ± 2248.18 vs 62 640.00 ± 5014.28,P < 0.01] and at 6 wk(AUC:29 956.60 ± 1757.33 vs 60 016.60 ± 3794.47,P < 0.01).In addition,insulin sensitivity was also significantly improved in mice treated with rAd-SP-rINSfur compared with rAd-CMV-βgal-treated mice(AUC:9150.17 ± 1007.78 vs 11 994.20 ± 474.40,P < 0.05).The islets from rAd-SP-rINSfur-injected mice appeared to be smaller and to contain a higher concentration of insulin than those from rAd-CMV-βgal-injected ***:Based on these results,we suggest that insulin gene therapy might be one therapeutic option for remission of type 2 diabetes.

关键词： Insulin gene therapy Synthetic promoter Glucose-responsive element Liver-specific promoter Type 2 diabetes

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Insulinotrophic and hypolipidemic effects of Ecklonia cava in streptozotocin-induced diabetic mice

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Asian Pacific Journal of Tropical Medicine 2012年第5期5卷 374-379页

作者： Mi-ja kim Hye Kyung kim Department of Food and Biotechnology Hanseo UniversitySeosan 356-706Republic of Korea Department of Pharmacology school of MedicineKyunghee UniversitySeoul 130-701Republic of Korea

Objective:To explore the anti-diabetic activity of Ecklonia cava(EC) in streptozotocin(STZ)-induced diabetic ***:Diabetes was induced by a single intraperitoneal injection of STZ(90 mg/kg).Normal and diabetic mice were treated with 0%,3%,and 5%EC diet for 4 weeks. Serum glucose and insulin concentrations,serum lipid profile,oral glucose tolerance test,and liver and pancreaticβ-cell histopathological observations were *** addition,in vitro glucose-induced insulin secretion was determined using pancreaticβ-islet ***:EC supplementation significantly and dose-dependently decreased serum glucose concentration, and improved glucose homeostasis in diabetic mice by preventing loss ofβ-cell mass resulting in increase of insulin *** triglyceride and total cholesterol concentrations in the serum and liver were markedly reduced by EC treatment in STZ-diabetic ***,LDL-,and HDL-cholesterol levels were ameliorated in EC supplemented diabetic *** steatosis induced by STZ was ameliorated by EC ***,in vitro insulinotrophic effect of EC extract was observed in pancreaticβ-***:This study demonstrated that EC is a potent and efficacious hypoglycemic and hypolipidemic agent,and prevents the loss ofβ-cell mass resulting in increase of insulin secretary capacity.

关键词： Diabetes Streptozotocin Insulin Lipid profile Pancreaticβ-cell

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