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Laparoscopic, Endoscopic and Robotic Surgery 2019年第4期2卷 99-101页

作者： Cuiyu Yang Dong Huang songying zhang Assisted Reproduction Unit Department of Obstetrics and GynecologySir Run Run Shaw HospitalZhejiang University School of MedicineKey Laboratory of Reproductive Dysfunction Management of Zhejiang ProvinceHangzhou 310016China

Chronic cornual ectopic pregnancy is rare.A 37-year-old woman,G1P0,presented with cornual mass,mild vaginal bleeding,and low serum human chorionic *** and cornual repair were performed by *** atypical symptoms,chronic... 详细信息

关键词： Chronic ectopic pregnancy Cornual Hemostasis Laparoscopy

The cervical function and pregnancy outcomes after hysteroscopic resection of the complete uterine septum,duplicate cervix and vaginal septum

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Laparoscopic, Endoscopic and Robotic Surgery 2019年第2期2卷 31-33页

作者： Dong Huang Lingyan zhang Jianmin Chen songying zhang Assisted Reproduction Unit Department of Obstetrics and GynecologySir Run Run Shaw HospitalZhejiang University School of Medicine/Key Laboratory of Reproductive Dysfunction Management of Zhejiang ProvinceHangzhou 310016China

Objective:To evaluate cervical function and pregnancy outcomes after hysteroscopic resection of the complete uterine septum,duplicate cervix and vaginal septum with Metzenbaum ***:Between January 2010 and December 2016,13 patients admitted to the Department of Obstetrics and Gynecology of Sir Run Run Shaw Hospital with complete uterine septum,duplicate cervix and vaginal septum,were enrolled into this *** cervical and corporal septum was cut by Metzenbaum scissors,and residual septum was cut by micro scissors under *** vaginal septum was cut with the unipolar electric ***:The operation time was about 10±1.31 *** the 13 patients present normal uterine cavities without scar formation under hysteroscopy at 3 months after operation,there was mild adhesions between anterior and posterior intrauterine wall on 2 *** operation,there were 13 pregnancies naturally conceived in 11 patients,10 *** live birth rate was 76.92%,the early miscarriage rate was 23.08%.The cesarean section ratewas 30%,the vaginal delivery ratewas 70%,and all were term ***:The operation was simple,convenient,and fast,without any complications and cervical *** was easy to have vaginal deliveries.

关键词： Hysteroscopic metroplasty Cervical function Pregnancy outcomes

Laparoscopic coring-type resection of noncommunicating broadly attached rudimentary horn with recurrent large ovarian endometriomas:A report of two cases

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Laparoscopic, Endoscopic and Robotic Surgery 2023年第1期6卷 31-34页

作者： Jianmin Chen Dong Huang Libing Shi songying zhang Assisted Reproduction Unit Department of Obstetrics and GynecologySir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province Hangzhou 310016China Zhejiang Provincial Clinical Research Center for Obstetrics and Gynecology Hangzhou 310016China

Rudimentary horn of the uterus is a rare congenital malformation that is characterized by significant anatomic *** report two cases involving women who presented with a broadly attached rudimentary horn and large ovarian endometriomas that were innovatively treated by laparoscopic coring-type dissection of the rudimentary horn of the *** novel surgical approach ensured the integrity of the myometrium of the unicornuate uterus and avoided uterine rupture during pregnancy and ipsilateral ovarian function impairment.

关键词： Laparoscopic Rudimentary horn Coring-type dissection Ovarian function

Prevention,diagnosis,and management of interstitial pregnancy:A review of the literature

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Laparoscopic, Endoscopic and Robotic Surgery 2019年第1期2卷 12-17页

作者： Jianmin Chen Dong Huang Libing Shi songying zhang Assisted Reproduction Unit Department of Obstetrics and GynecologySir Run Run Shaw HospitalZhejiang University School of MedicineKey Laboratory of Reproductive Dysfunction Management of Zhejiang ProvinceHangzhou 310016China

Objective:The incidence of interstitial pregnancy(IP)has increased by using assisted reproductive technology,especially after ipsilateral *** review aimed to examine the current state of prevention,risk factors,diagnosis,and management of IP,based on the current *** sources:We searched PubMed for English language articles published up to June *** search terms included interstitial pregnancy,ectopic pregnancy or cornual pregnancy,angular pregnancy,and combinations of these *** selection:Articles were obtained and reviewed to analyze risk factors,diagnosis,prevention,and management of ***:Ipsilateral salpingectomy is the main high-risk factor of IP,especially after in vitro *** suture at the time of salpingectomy helps to reduce the IP *** cornuostomy appears to be an effective treatment in intact cases,although it sometimes needs to be combined with methotrexate therapy in ruptured cases complicated by persistent disease.

关键词： Laparoscopic cornuostomy Interstitial pregnancy Cornual suture Salpingectomy

Effect of modified laparoscopic salpingectomy on ovarian reserve:Changes in the serum antimüllerian hormone levels

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Laparoscopic, Endoscopic and Robotic Surgery 2019年第1期2卷 8-11页

作者： Dong Huang Yunshan Zhu Jianmin Chen songying zhang Assisted Reproduction Unit Department of Obstetrics and GynecologySir Run Run Shaw HospitalZhejiang University School of MedicineKey Laboratory of Reproductive Dysfunction Management of Zhejiang ProvinceHangzhou 310016China

Objective:To retrospectively estimate the impact of modified laparoscopic salpingectomy on the ovarian reserve in infertile ***:There were 74 infertile women undergone modified laparoscopic salpingectomy from June 2016 to January 2017,and their levels of serum antimüllerian hormone,basal follicle-stimulating hormone and estradiol were reviewed ***:No significant change was detected in serum antimüllerian hormone at 3 months after surgery compared to preoperative level(p=0.857).Similar changes were observed for the basal follicle-stimulating hormone(p=0.102)and estradiol(p=0.233)***:Our results revealed that modified laparoscopic salpingectomy might be a valuable treatment for hydrosalpinx before in vitro fertilization.

关键词： Modified laparoscopic salpingectomy Ovarian reserve Serum antimüllerian hormone

Successful delivery of unicornuate uterus pregnancy after laparoscopic cervical cerclage: A case report

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Laparoscopic, Endoscopic and Robotic Surgery 2021年第4期4卷 125-127页

作者： Cuiyu Yang Dong Huang Jingyan Yang Yang Yang songying zhang Assisted Reproduction Unit Department of Obstetrics and GynecologySir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province Hangzhou 310016China

There are few reports of the successful delivery of unicornuate uterus pregnancy after laparoscopic cervical ***,we report a 32-year-old woman,gravida 2 para 1,who had been admitted for unicornuate uterus pregnancy by a thawed frozen blastocyst *** accepted laparoscopic cervical cerclage for cervical insufficiency and delivered successfully via caesarean section at 35^(+3) *** cerclage may be used as an effective method of preventing abortion in unicornuate uterus pregnancy,while surgery by laparoscopy would be a better choice for patients with poor cervical *** should pay due attention to complications such as uterine rupture for these highrisk patients.

关键词： Unicornuate uterus Cervical cerclage Cervical insufficiency

In situ delivery of apoptotic bodies derived from mesenchymal stem cells via a hyaluronic acid hydrogel:A therapy for intrauterine adhesions

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Bioactive Materials 2022年第6期7卷 107-119页

作者： Liaobing Xin Cheng Wei Xiaomei Tong Yangyang Dai Dong Huang Jianmin Chen Lie Ma songying zhang Assisted Reproduction Unit Department of Obstetrics and GynecologySir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou310016China MOE Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou310027China Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province.No.3 Qingchun East Road Jianggan DistrictHangzhou310016China

Stem cell-based and stem cell-derived exosome-based therapies have shown promising potential for endometrial regeneration and the clinical treatment of intrauterine adhesions(IUAs).Evidence shows that apoptosis occurs in a majority of grafted stem cells,and apoptotic bodies(ABs)play a critical role in compensatory tissue ***,the therapeutic potential of AB-based therapy and its mechanism have not been explored in ***,a cell-free therapeutic strategy was developed by incorporating mesenchymal stem cell-derived ABs into a hyaluronic acid(HA)hydrogel to achieve endometrial regeneration and fertility ***,we found that the ABs could induce macrophage immunomodulation,cell proliferation,and angiogenesis in *** HA hydrogel promoted the retention of ABs and facilitated their continuous *** a murine model of acute endometrial damage and a rat model of IUAs,in situ injection of the AB-laden HA hydrogel could efficiently reduce fibrosis and promote endometrial regeneration,resulting in the fertility ***,ABs show good potential as therapeutic vesicles,and the AB-laden HA hydrogel appears to be a clinically feasible and cell-free alternative for endometrial regeneration and IUA treatment.

关键词： Apoptotic bodies Mesenchymal stem cells Endometrial regeneration Intrauterine adhesions Tissue engineering

Outcomes of patients who have undergone laparoscopic abdominal cerclage:A retrospective study

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Laparoscopic, Endoscopic and Robotic Surgery 2022年第3期5卷 111-115页

作者： Cuiyu Yang Dong Huang Yang Yang Jingyan Yang Yuyang Chen Mei Pan songying zhang Assisted Reproduction Unit Department of Obstetrics and GynecologySir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China

Objective:This study aims to evaluate the surgical morbidity and obstetric outcomes following in-pregnancy or pre-pregnancy laparoscopic abdominal cerclage(LAC)for patients who were diagnosed with refractory cervical insufficiency or had a short ***:A retrospective study was conducted on patients undergoing LAC between May 2017 and May 2019 at the Department of Obstetrics and Gynecology,Sir Run Run Shaw *** patients were diagnosed with refractory cervical insufficiency based upon a previous failed transvaginal cervical cerclage(TVC),or had a short cervix who were considered unsuitable for a TVC after a previous cervical *** patients were followed-up after surgery with transperineal ultrasonography until May ***,surgical and obstetric data were collected and ***:In total,44 patients underwent LAC,with 8 patients in-pregnancy and 36 *** the patient with pre-pregancy LAC,the pregnancy rate was 80.6%(29/36),including 3 patients with first-trimester loss,1 patient with an ectopic pregnancy,and 25 patients with a *** the remaining 7 patients,3 did not conceive,and another 4 had no pregnancy *** the patients with in-pregnancy LAC had a ***“take-home baby”rate was 89.2%(33/37),with a live-birth rate of 100%and a neonatal survival rate of 100%for both patients with in-pregnancy and pre-pregnancy *** patients with in-pregnancy LAC,75.0%(6/8)patients delivered at≥37 wk of gestation,12.5%(1/8)delivered between 34 and 36^(+6)wk,and 12.5%(1/8)delivered between 28 and 33^(+6)*** patients with pre-pregnancy LAC,80.0%(20/25)patients delivered at≥37 wk of gestation,16.0%(4/25)delivered between 34 and 36^(+6)wk,and 4.0%(1/25)delivered between 28 and 33^(+6)*** adverse-event intra-operative or post-operative sequelae were ***:LAC is an effective and safe procedure that results in remarkable obstetric outcomes for women with refractory cervical insufficiency,or with a sh

关键词： Cervical cerclage Cervical insufficiency Laparoscopic Conization Preterm birth

A construct of adipose-derived mesenchymal stem cells—laden collagen scaffold for fertility restoration by inhibiting fibrosis in a rat model of endometrial injury

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Regenerative Biomaterials 2023年第1期10卷 1481-1495页

作者： Yangyang Dai Liaobing Xin Sentao Hu Shiqian Xu Dong Huang Xiaoying Jin Jianmin Chen Rachel Wah Shan Chan Ernest Hung Yu Ng William Shu Biu Yeung Lie Ma songying zhang Assisted Reproduction Unit Department of Obstetrics and GynecologySir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Key Laboratory of Reproductive Dysfunction Management of Zhejiang Province Hangzhou 310016China MOE Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China Department of Obstetrics and Gynaecology School of Clinical MedicineLKS Faculty of MedicineThe University of Hong KongHong Kong SAR 999077China Shenzhen Key Laboratory of Fertility Regulation The University of Hong Kong Shenzhen HospitalShenzhen 518000China

Severe endometrium damage causes pathological conditions such as thin endometrium and intrauterine adhesion,resulting in uterine factor *** stem cell(MSC)therapy is a promising strategy in endometrial repair;yet,exogenous MSCs still raise concerns for safety and ethical *** adipose-derived mesenchymal stem cells(ADMSCs)residing in adipose tissue have high translational potentials due to their autologous *** harness the high translation potentials of ADMSC in clinical endometrium regeneration,here we constructed an ADMSCs composited porous scaffold(CS/ADMSC)and evaluated its effectiveness on endometrial regeneration in a rat endometrium-injury *** found that CS/ADMSC intrauterine implantation(i)promoted endometrial thickness and gland number,(ii)enhanced tissue angiogenesis,(iii)reduced fibrosis and(iv)restored *** ascertained the pro-proliferation,pro-angiogenesis,immunomodulating and anti-fibrotic effects of CS/ADMSC in vitro and revealed that the CS/ADMSC influenced extracellular matrix composition and organization by a transcriptomic *** results demonstrated the effectiveness of CS/ADMSC for endometrial regeneration and provided solid proof for our future clinical study.

关键词： adipose-derived mesenchymal stem cells collagen scaffold endometrium fibrosis regeneration

A comparison of implantation, miscarriage and pregnancy rates of single and double day 3 embryo transfer between fresh and frozen thawed transfer cycles： a retrospective study

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Chinese Medical Journal 2014年第5期127卷 911-915页

作者： Liu Liu Tong Xiaomei Jiang Lingying Tinchiu Li Zhou Feng zhang songying Center of Reproductive Medicine Sir Run Run Shaw HospitalSchool of Medicine Zhejiang University Hangzhou Zhejiang 310006 China Department of Reproductive Medicine Jessop Wing Royal Hallamshire Hospital University of Sheffield UK

Background Reduced endometrial receptivity in hyperstimulated cycles may lead to a lower implantation rate and a lower clinical pregnancy rate,but it is unclear if it is also associated with an increase in pregnancy loss *** aim of this study was to compare the implantation,miscarriage,and pregnancy rates between fresh and frozen thawed transfer of one or two day-3 embryos,with a view to understanding whether or not reduced endometrial receptivity encountered in hyperstimulated cycles is associated with an increase in miscarriage *** This study involved a consecutive series of 1 551 single day-3 embryo transfer cycles and consecutive 5 919 double day-3 embryo transfer cycles in the Assisted Reproductive Unit of the Sir Run Run Shaw Hospital,Hangzhou,China,between January 2010 and December *** The implantation and clinical pregnancy rates （single embryo 30.7％ and double embryos 33.4％ and 51.4％）using fresh cycle were both significantly lower than that of frozen-thawed cycles （single embryo 35.8％ and double embryos 38.1％ and 57.8％）.There was no difference in biochemical loss or clinical miscarriage rates between the two *** Impairment of endometrial receptivity associated with ovarian hyperstimulation leads to implantation failure at a very early stage,resulting in an increased number of *** does not lead to increase in biochemical or clinical *** significantly reduced ongoing pregnancy rates in both fresh single and double embryo transfer are therefore due to failure to achieve a pregnancy,rather than pregnancy loss after conception.

关键词： pregnancy loss miscarriage in vitro fertilization embryo transfer implantation rate