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黄芪甲苷对肥胖型多囊卵巢综合征大鼠胰岛素抵抗及MAPK/ERK通路的影响

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中成药 2024年第1期46卷 94-100页

作者：刘冷贺春花荆门市中心医院妇科湖北荆门448001

目的研究黄芪甲苷对肥胖型多囊卵巢综合征(PCOS)大鼠胰岛素抵抗(IR)的改善作用,并分析对其卵巢MAPK/ERK通路的影响。方法来曲唑联合高脂高糖饮食法构建肥胖PCOS大鼠模型,随机分为模型组、二甲双胍(135 mg/kg)组和低、高剂量黄芪甲苷(25... 详细信息

目的研究黄芪甲苷对肥胖型多囊卵巢综合征(PCOS)大鼠胰岛素抵抗(IR)的改善作用,并分析对其卵巢MAPK/ERK通路的影响。方法来曲唑联合高脂高糖饮食法构建肥胖PCOS大鼠模型,随机分为模型组、二甲双胍(135 mg/kg)组和低、高剂量黄芪甲苷(25、50 mg/kg)组,每组8只,另设对照组,连续灌胃给药21 d后,记录体质量,计算卵巢指数,测定空腹血糖(FBG)、血清甘油三酯(TG)、总胆固醇(TC)、促卵泡激素(FSH)、睾酮(T)、雌二醇(E_(2))、促黄体生成素(LH)及空腹胰岛素(FINS)水平,计算HOMA-IR和LH/FSH值,HE染色观察卵巢组织形态,Western blot法检测卵巢组织MAPK/ERK通路相关蛋白表达,免疫荧光法检测卵巢组织血管内皮生长因子(VEGF)蛋白表达。结果与对照组比较,模型组大鼠卵巢形态呈多囊样病态改变,体质量、卵巢指数、血清TG、TC、LH、FSH、T、FINS、FBG水平、LH/FSH和HOMA-IR值、卵巢组织p-ERK1/2/ERK1/2、p-MEK1/2/MEK1/2、p-Raf/Raf、VEGF蛋白表达升高(P<0.01),血清E_(2)水平降低(P<0.01);与模型组比较,黄芪甲苷各剂量组和二甲双胍组大鼠卵巢多囊样病变明显缓解,体质量、卵巢指数、血清TG、TC、LH、FSH、T、FINS、FBG水平、LH/FSH和HOMA-IR值、卵巢组织p-ERK1/2/ERK1/2、p-MEK1/2/MEK1/2、p-Raf/Raf、VEGF蛋白表达降低(P<0.05,P<0.01),血清E_(2)水平升高(P<0.05,P<0.01)。结论黄芪甲苷可拮抗肥胖PCOS大鼠IR,改善激素水平,减轻其卵巢病变,该作用可能与抑制MAPK/ERK通路活化有关。

关键词：黄芪甲苷多囊卵巢综合征肥胖胰岛素抵抗激素 MAPK/ERK通路

CNKSR2 interactome analysis indicates its association with the centrosome/microtubule system

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Neural Regeneration Research 2025年第8期20卷 2420-2432页

作者： Lin Yin Yalan Xu Jie Mu Yu leng Lei Ma Yu Zheng Ruizhi Li Yin Wang Peifeng Li Hai Zhu Dong Wang Jing Li Institute for Translational Medicine The Affiliated Hospital of Qingdao UniversityMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina School of Basic Medicine Qingdao UniversityQingdaoShandong ProvinceChina College of Life Sciences and School of PharmacyMedical CollegeQingdao UniversityQingdaoShandong ProvinceChina Department of Urology Qingdao Municipal Hospital Affiliated to Qingdao UniversityQingdaoShandong ProvinceChina

The protein connector enhancer of kinase suppressor of Ras 2(CNKSR2),present in both the postsynaptic density and cytoplasm of neurons,is a scaffolding protein with several protein-binding *** of the CNKSR2 gene have been implicated in neurodevelopmental disorders,particularly intellectual disability,although the precise mechanism involved has not yet been fully *** has demonstrated that CNKSR2 plays a role in facilitating the localization of postsynaptic density protein complexes to the membrane,thereby influencing synaptic signaling and the morphogenesis of dendritic ***,the function of CNKSR2 in the cytoplasm remains to be *** this study,we used immunoprecipitation and high-resolution liquid chromatography-mass spectrometry to identify the interactors of *** a combination of bioinformatic analysis and cytological experiments,we found that the CNKSR2 interactors were significantly enriched in the proteome of the *** also showed that CNKSR2 interacted with the microtubule protein DYNC1H1 and with the centrosome marker *** colocalization analysis confirmed the centrosomal localization of *** we downregulated CNKSR2 expression in mouse neuroblastoma cells(Neuro 2A),we observed significant changes in the expression of numerous centrosomal *** manipulation also affected centrosome-related functions,including cell size and shape,cell proliferation,and ***,we found that CNKSR2 interactors were highly enriched in de novo variants associated with intellectual disability and autism spectrum *** findings establish a connection between CNKSR2 and the centrosome,and offer new insights into the underlying mechanisms of neurodevelopmental disorders.

关键词： autism spectrum disorder centrosome CNKSR2 intellectual disability interactome mass spectrometry microtubule neurodevelopmental disease protein complexes protein-protein interactions

岩洞处置库围岩胶体对Sr^(2+)的吸附性能研究

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核技术 2024年第7期47卷 32-40页

作者：阳刚冷阳春沈统庹先国成都大学成都610106 西南科技大学绵阳621010 成都理工大学成都610059

岩洞处置环境中,围岩作为阻滞放射性核素进入环境的最后一道屏障,其在长期处置过程中产生的围岩胶体增大了核素迁移的风险。本文以围岩胶体和处置库拟处置的Sr^(2+)为研究对象,开展了围岩胶体稳定性及胶体对Sr^(2+)的吸附性能研究。稳... 详细信息

岩洞处置环境中,围岩作为阻滞放射性核素进入环境的最后一道屏障,其在长期处置过程中产生的围岩胶体增大了核素迁移的风险。本文以围岩胶体和处置库拟处置的Sr^(2+)为研究对象,开展了围岩胶体稳定性及胶体对Sr^(2+)的吸附性能研究。稳定性研究表明:pH、温度及岩洞处置库地下水中部分离子均对围岩稳定性有影响,围岩胶体在处置库地下水环境中拥有良好的稳定性能。通过吸附试验,考察了时间、pH、不同离子等因素对吸附的影响,并对其吸附动力学及吸附等温模型进行了分析。结果显示:围岩胶体吸附Sr^(2+)在12 h达到平衡,平衡吸附量为41.79 mg·g^(-1);碱性环境下,吸附量随pH而增大;不同离子均对围岩胶体吸附Sr^(2+)有抑制作用,其阳离子抑制作用大于阴离子。围岩胶体对Sr^(2+)的吸附符合准二级动力学模型及Freundlich吸附等温模型。

关键词：岩洞处置库围岩胶体 Sr^(2+) 吸附

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基于微分博弈的草原放牧管理策略研究

干旱区资源与环境 2024年第1期38卷 1-8页

作者：冷杰祁新曹锃东北财经大学管理科学与工程学院大连116025 浙江大学生命科学学院杭州310058 杭州应用声学研究所杭州311400

当前,草原畜牧业发展与草原生态可持续保护间的矛盾日益凸显,如何协调二者之间的关系成为了当前草原管理的重要内容。考虑引入生态补偿政策,构建了政府草原放牧管理与牧民放牧决策的微分博弈模型,探讨了双方的动态最优决策以及草原生态... 详细信息

当前,草原畜牧业发展与草原生态可持续保护间的矛盾日益凸显,如何协调二者之间的关系成为了当前草原管理的重要内容。考虑引入生态补偿政策,构建了政府草原放牧管理与牧民放牧决策的微分博弈模型,探讨了双方的动态最优决策以及草原生态水平的演变规律,阐释了生态补偿政策的效用机理。研究表明:在草原放牧管理中,政府和牧民采取状态依赖的动态决策是实现草畜平衡、最大化利用草原资源的有效途径;政府实施草原生态补偿政策可以在保障牧民收益的同时取得更好的草原生态保护成效;在草原生态补偿政策推行过程中,增加生态补偿力度可以有效激励牧民合理减牧,进而促进草原生态水平的提升。

关键词：放牧策略草原生态水平生态补偿微分博弈

Thrombus migration in patients with acute ischaemic stroke undergoing endovascular thrombectomy

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Stroke & Vascular Neurology 2024年第2期9卷 126-133,I0006-I0395页

作者： ZeFeng Tan Lei Zhang Li'an Huang Hongyu Qiao Min Guan Bing Yang Pengfei Yang Yongwei Zhang Hongjian Shen Yu Zhou Bo Hong Huaizhang Shi Hongxing Han Xinyi leng Yi Dong Changlin Lian Wenhuo Chen Anding Xu Jianmin Liu Neurology First People's Hospital of FoshanFoshanGuangdongChina Department of Neurology Jinan University First Affiliated HospitalGuangzhouGuangdongChina Neurovascular Center Changhai HospitalNaval Medical UniversityShanghaiChina Department of Neurosurgery First Affiliated Hospital of Harbin Medical UniversityHarbinChina Department of Neurology Linyi People's HospitalLinyiShandongChina Medicine&Therapeutics The Chinese University of Hong KongHong KongChina Neurology Huashan Hospital Fudan UniversityShanghaiChina Neurology Zhangzhou Municipal Hospital of Fujian Province and Zhangzhou Affiliated Hospital of Fujian Medical UniversityZhangzhouFujianChina Stroke Center Jinan University First Affiliated HospitalGuangzhouGuangdongChina

Objective The impact of thrombus migration(TM)prior to endovascular thrombectomy(EVT)on clinical outcomes and revascularisation rates remains *** aimed to examine whether preinterventional TM modifies the treatment effects of direct EVT versus bridging EVT in acute large vessel occlusion *** All patients undergoing catheter angiography in the Direct Intra-arterial thrombectomy in order to Revascularise acute ischaemic stroke patients with large vessel occlusion Efficiently in Chinese Tertiary hospitals:A Multicentre randomised clinical Trial were *** was determined by radiologists unaware of the study by analysing discrepancies between computed tomographic angiography at baseline and first-run digital subtraction angiography before *** primary outcome was the score on the modified Rankin scale(mRS)assessed at 90 *** Of 627 included patients,the TM rate was 11.3%(71/627).In the multivariable logistic regression model,baseline National Institutes of Health Stroke Scale score(adjusted OR 0.956,95%CI 0.916 to 0.999;p=0.043)and intravenous thrombolysis(adjusted OR 2.614,95%CI 1.514 to 4.514;p<0.001)were independently associated with *** patients with TM were less likely to be completely recanalised than those without TM(21.27%vs 36.23%,p=0.040).The interaction of TM and the EVT treatment effect did not significantly affect mRS shift analysis(p=0.687)or mRS scores of 0 to 1(p=0.436).Conclusion Preinterventional TM does not modify the treatment effects of direct versus bridging EVT on functional outcomes in patients with acute ischaemic stroke with anterior large vessel *** leads to a lower complete recanalisation rate.

关键词： patients throm acute

Gallic acid ameliorates colitis by modulating the Treg/Th17 balance via microbiota-derived secondary bile acids

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Food Science and Human Wellness 2024年

作者： Xiao Zhang Yun leng Qian Zhang Hansheng Yu Ziqiong Liao Jiaxuan Xia Chong Ma Kun Liu Yuefeng Zhang Yanjun Hong Hao Li Zhiyong Xie School of Pharmaceutical Sciences (Shenzhen)， Sun Yat-sen University The Seventh Affiliated Hospital， Sun Yat-sen University Shenzhen Key Laboratory of Chinese Medicine Active Substance Screening and Translational Research， Sun Yat-sen University

Gallic acid (GA)， a plant phenol ubiquitously present in fruits and vegetables， has demonstrated efficacy in ameliorating ulcerative colitis (UC). Despite previous reports， the precise mechanism of GA's therapeutic action remains elusive. Herein， the present work aims to delineate the mechanism underlying the anti-UC effects of GA by focusing on the interplay of gut microbiota， microbial and host cometabolites， and gut immune regulation. The findings revealed that GA treatment improved the colitis symptoms and systematic inflammatory response， reliant on gut microbiota， as evidenced by microbiota depletion and fecal microbiota transplantation. According to the 16s rDNA sequencing results， GA altered the gut microbiota community structure and upregulated the biosynthesis of secondary bile acids (SBAs). Metabolomics and flow cytometry analysis (FCS) revealed a substantial increase in SBAs production， including UDCA， LCA， 3-oxo-LCA and IsoalloLCA， which further upregulated the proportion of regulatory T (Treg) cells and downregulated the proportion of T helper type 17 (Th17) cells in the colon， ultimately resulting in an improved Treg/Th17 balance. Further FCS and real-time quantitative PCR assays provided mechanistic insights， demonstrating that UDCA and IsoalloLCA facilitate Treg cell differentiation through the upregulation of nuclear hormone receptor 4A1 (NR4A1). This research elucidated that GA effectively mitigates colitis by modulating the Treg/Th17 balance， facilitated by the enhanced synthesis of microbiota-derived SBAs. These insights unveil innovative pathways through which GA exerts its anti-UC effects， emphasizing the potential therapeutic benefits of incorporating a GA-enriched diet into UC management.

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主宾易位的语义制约和语用选择

语言研究 2024年第2期44卷 1-10页

作者：孙道功冷梅洁南京师范大学文学院南京210097

主宾易位句作为一种特殊句式,长期以来受到汉语学界的关注。文章在回溯梳理相关研究成果的基础上,从语义和语用两个维度,考察了主宾易位的语义制约特点及语用选择机制。结论认为:语义制约表现为对立性特征模糊化、动核的动作性弱化、语... 详细信息

主宾易位句作为一种特殊句式,长期以来受到汉语学界的关注。文章在回溯梳理相关研究成果的基础上,从语义和语用两个维度,考察了主宾易位的语义制约特点及语用选择机制。结论认为:语义制约表现为对立性特征模糊化、动核的动作性弱化、语义指向的双向化。语用选择机制表现为主题和焦点的转换、语境的适应性、语篇的动态选择性的制约。主宾易位是语义和语用互动作用的结果,认知是语义和语用的关联纽带。在语义层面,句式中虽然存在多重语义关系,但是在人的认知域中最基础的语义关系具有固定性。在语用层面,言语交际中所表达的句式需要依据交际双方共有的背景知识语境和认知能力进行推理从而获得符合特定语境的语用解释。

关键词：主宾易位句语义制约语用选择互动机制

中国区域环境协同治理的“适应性嵌套”逻辑--基于社会生态系统框架的分析

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理论与改革 2024年第5期 109-128页

作者：锁利铭冷雪忠南开大学周恩来政府管理学院南开大学中国政府发展联合研究中心

区域环境协同治理是中国生态环境保护领域的重大实践变迁,既有研究多从工具视角出发探讨区域环境协同治理的优化路径,对其内在逻辑则缺少系统分析。文章以社会生态系统框架为参照系,从外部情景、内部系统与交互效应三个维度阐释中国区... 详细信息

区域环境协同治理是中国生态环境保护领域的重大实践变迁,既有研究多从工具视角出发探讨区域环境协同治理的优化路径,对其内在逻辑则缺少系统分析。文章以社会生态系统框架为参照系,从外部情景、内部系统与交互效应三个维度阐释中国区域环境协同治理的整体逻辑。中国区域环境协同治理呈现适应性嵌套的逻辑进路:应对跨域性风险与治理碎片化困境、区域战略与环境政策双重演化、经济区域化、环境正义需求等外部情景,中国区域环境治理内部系统在环境产权分置、环境治理单元扩展、社会公众环境参与、中央政府统筹协调等交互作用下,产生了治理结构优化与环境效益改善的反馈型协同效应路径。应对日益复杂的区域环境治理议题,需要将真实世界的“社会生态系统”映射于理论研究的“社会生态治理系统”。未来相关研究要注重构建并检验中国式区域环境协同治理分析框架、探索复杂系统管理视角下的区域环境治理共同体理念、设计“情景—手段”动态匹配下的区域环境跨界治理方法、审视网络分析在区域环境协同治理研究中的工具价值。

关键词：区域环境治理生态环境协同治理社会生态系统复杂性适应性嵌套复杂系统

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Morphological,phylogenetic and metabolite profile of Prorocentrum clipeus,a newly recorded epiphytic dinoflagellate in the northern Yellow Sea

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Acta Oceanologica Sinica 2024年第8期43卷 128-141页

作者： Ruifang Wang Mengmeng Tong Shiwen Zhou Junjie Zheng Wenguang Zhang Xinfeng Dai Douding Lu Jiarong Hu Tianze leng Qinglin Mu Zhongyong Yan Jiangning Zeng Pengbin Wang Ocean College Zhejiang UniversityZhoushan 316021China Key Laboratory of Marine Ecosystem Dynamics Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China The State Key Laboratory of Marine Pollution Department of Biomedical SciencesCity University of Hong KongHong Kong SAR 999077China Zhejiang Marine Ecology and Environment Monitoring Center Zhoushan 316000China

More than 30 species of benthic Prorocentrum have been identified,some of which produce okadaic acid(OA)and its derivatives,dinophysistoxins(DTXs),which cause diarrhetic shellfish poisoning(DSP).Increasing numbers of benthic Prorocentrum species have been reported in tropical and subtropical waters of *** contrast,only a few benthic Prorocentrum species have been reported in temperate *** this study,morphological descriptions obtained using light microscopy,scanning electron microscopy and molecular characterization of one Prorocentrum clipeus strain isolated from the Yellow Sea are *** clipeus cells were nearly circular in shape,with a collar,ridge,and one *** periflagellar area was wide U-shaped,with two curved projections on platelet *** periflagellar platelets of different sizes were *** morphology closely fits that of the species isolated from other *** analysis based on the molecular sequences of the small subunit(SSU)rDNA,internal transcribed spacer(ITS),and large subunit(LSU)rDNA was performed.A comprehensive metabolomic analysis incorporating target,suspect and non-target screenings was first applied to investigate the intracellular and extracellular metabolite profiles of the current isolate of *** to the results of the target and suspect screenings,179 metabolites or toxins produced by DSP-related algal species,including OA,dinophysistoxin-1(DTX1),dinophysistoxin-2(DTX2)and pectenotoxin-2(PTX2),were not ***-target screening involving feature-based molecular networking(FBMN)provided a global view of major metabolites produced by the *** DF128 strain and revealed 23 clusters belonging to at least 13 compound classes,with organometallic compounds,lipids and lipid-like molecules,phenylpropanoids and polyketides,and benzenoids as major *** date,this is the first record of the characterization of *** in samples from Chinese *** results suppo

关键词： Prorocentrum taxonomy phylogeny metabolite profiling Yellow Sea