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中国海洋大学学报（自然科学版） 2023年第8期53卷 123-131页

作者：吴松源赵洋刘勇中国海洋大学山东省海洋工程重点实验室山东青岛266100

为有效提高挡板式防波堤的掩护效果,同时抑制基床冲刷,本文提出了一种新型的挡板-堆石防波堤结构,并利用理论解析的方法,对其透、反射特性进行了研究。基于线性势流理论,通过多项Galerkin方法模拟垂直挡板两侧的压力差,建立边值问题,利... 详细信息

为有效提高挡板式防波堤的掩护效果,同时抑制基床冲刷,本文提出了一种新型的挡板-堆石防波堤结构,并利用理论解析的方法,对其透、反射特性进行了研究。基于线性势流理论,通过多项Galerkin方法模拟垂直挡板两侧的压力差,建立边值问题,利用匹配特征函数法进行解析求解。通过结果收敛性分析和正确性验证,发现解析解收敛性良好,且与分区边界元方法的计算结果一致。基于算例分析,研究了挡板-堆石防波堤的结构参数对防波堤掩护效果的影响,为实际工程提供科学指导。

关键词：挡板-堆石防波堤消浪特性透射系数反射系数多项Galerkin方法匹配特征函数

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Comparative Analysis of the Integument Transcriptomes between Stick Mutant and Wild-Type Silkworms

Comparative Analysis of the Integument Transcriptomes betwee...

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中国蚕学会第十届青年学术研讨会

作者： Duan Tan Hai Hu Xiaoling Tong Minjin Han songyuan wu Xin Ding Fangyin Dai Cheng Lu State Key Laboratory of Silkworm Genome Biology Key Laboratory of Sericultural Biology and Genetic BreedingMinistry of AgricultureCollege of BiotechnologySouthwest University

In insects,the integument provides mechanical support for the whole body and protects them from infections,physical and chemical injuries,and *** in integument properties is often related to body shape,behavior,and survival *** stick（sk） silkworm is a spontaneous mutant with a stick-like larval body that is firm to the touch and,thus,less *** of the mechanical properties of the cuticles at day 3 of the fifth instar（L5D3） of sk larvae revealed higher storage modulus and lower loss *** sequencing identified a total of 19,969 transcripts that were expressed between wild-type Dazao and the sk mutant at L5D2,of which 11,596 transcripts were novel and detected in the *** expression analyses identified 710 upregulated genes and 1009 downregulated genes in the sk *** Ontology（GO） enrichment analysis indicated that four chitin-binding peritrophin A domain genes and a chitinase gene were upregulated,whereas another four chitin-binding peritrophin A domain genes,a trehalase,and nine antimicrobial peptides were *** Encyclopedia of Genes and Genomes（KEGG） analysis indicated that two functional pathways,namely,fructose and mannose metabolism and tyrosine metabolism,were significantly enriched with differentially-expressed *** study provides a foundation for understanding the molecular mechanisms underlying the development of the stiff exoskeleton in the sk mutant.

关键词： Bombyx mori stick mutant transcriptome integument stiff exoskeleton

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Morphological characterization and molecular mapping of an irradiation-induced Speckled mutant in Silkworm,Bombyx mori

Morphological characterization and molecular mapping of an i...

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中国蚕学会第九届青年学术研讨会

作者： Duan Tan Xiaoling Tong Hai Hu songyuan wu Chunlin Li Gao Xiong Zhonghuai Xiang Fangyin Dai Cheng Lu State Key Laboratory of Silkworm Genome Biology Southwest University Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry Southwest University

Speckled（Spc）,an X-ray-induced lethal mutant of Bombyx mori,exhibits a mosaic dark-brown-spotted larval epidermis in both sexes and egg-laying problems only in ***,we report the morphological characterization and molecular mapping of the Spc *** investigations revealed that the epidermal ultrastructure of the small,dark-brown spots was more dense than that of the white regions in both Spc/+ mutants and wild type,and that the lethality of the Spc/Spc mutants occurred during early ***,the ovarioles and ovipositor were disconnected in approximately 85.5% of Spc/+ females,a further 2.5% had a connection between the ovarioles and ovipositor that was too narrow to lay *** remaining females showed a normal connection similar to that of the wild *** successfully narrowed down the location of the Spc mutation to a region on chromosome 4 that was ～1041 kb ***-prediction analysis identified 25 candidate genes in this *** structure analysis indicated that a ～305-kb deletion was included in the mapping *** and spatial reverse transcription（RT-PCR） analysis showed that several genes in the mapped region are associated with the Spc *** the genes responsible for the Spc mutation were not definitively identified,our results further the current understanding of the complex mechanism underlying the multiple morphological defects in Spc mutants.

关键词： Bombyx mori Speckled mutant phenotypic analysis molecular mapping autosomal fragment deletion

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Disruption of PTPS Gene Causing Pale Body Color and Lethal Phenotype in the Silkworm,Bombyx mori

Disruption of PTPS Gene Causing Pale Body Color and Lethal P...

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中国蚕学会第十届青年学术研讨会

作者： Xiaoling Tong Pingfeng Liang songyuan wu Yuanhao Li Liang Qiao Hai Hu Zhonghuai Xiang Cheng Lu Fangyin Dai State Key Laboratory of Silkworm Genome Biology Key Laboratory of Sericulture Biology and Genetic BreedingAgricultural MinistryCollege of BiotechnologySouthwest University College of Biotechnology Southwest University

Phenylketonuria（PKU） is an inborn error of metabolism caused by mutations in the phenylalanine hydroxylase（PAH） gene or by defects in the tetrahydrobiopterin（BH4） synthesis ***,by positional cloning,we report that the 6-pyruvoyl-tetrahydropterin synthase（PTPS） gene,encoding a key enzyme of BH4 biosynthesis,is responsible for the alc（albino C） mutation that displays pale body color,head shaking,and eventually lethality after the first molting in *** to wild type,the alc mutant produced more substrates（phenylalanine（Phe） and tyrosine（Tyr）） and generated less DOPA and *** of 2,4-diamino-6-hydroxypyrimidine（DAHP） to block BH4 synthesis in the wild type effectively produced the alc-like phenotype,while BH4 supplementation rescued the defective body color and lethal phenotype in both alc and DAHP-treated *** detection of gene expressions and metabolic substances after drugs treatments in alc and normal individuals imply that silkworms and humans have a high similarity in the drugs metabolic features and the gene pathway related to BH4 and the dopamine *** propose that the alc mutant could be used as an animal model for drug evaluation for BH4-deficient PKU.

关键词： silkworm model alc PTPS BH4 phenylketonuria

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家蚕着色模式相关的分子遗传学研究

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蚕业科学 2016年第3期42卷 373-385页

作者：彭晨星吴松原代方银家蚕基因组生物学国家重点实验室农业部蚕桑功能基因组与生物技术重点实验室西南大学生物技术学院重庆400715

体色与斑纹是昆虫重要的形态特征,对于昆虫捕食、避敌、寻偶、免疫和调节体温等具有重要的意义,其丰富着色模式形成的分子机制一直是昆虫学研究的热点。家蚕作为中国重要的农业经济昆虫,具有5 000多年的驯养历史,不仅成就了灿烂的丝绸文... 详细信息

体色与斑纹是昆虫重要的形态特征,对于昆虫捕食、避敌、寻偶、免疫和调节体温等具有重要的意义,其丰富着色模式形成的分子机制一直是昆虫学研究的热点。家蚕作为中国重要的农业经济昆虫,具有5 000多年的驯养历史,不仅成就了灿烂的丝绸文化,而且带来了巨大的经济效益。家蚕亦是鳞翅目的模式昆虫,拥有的超过200余个与着色相关的突变体是昆虫着色研究的重要实验材料。近年来,结合家蚕的经典遗传连锁图谱、家蚕基因组数据,运用高通量测序手段、定位克隆策略等分离鉴定家蚕着色相关突变体的关键基因,是探索着色模式形成的分子基础的主体。目前已在分子水平解析了家蚕30余种着色相关突变的产生机制,显著推动了家蚕着色机制的研究,对于丰富昆虫着色相关理论和基础知识具有重要贡献。本文总结了家蚕着色模式研究的最新成果,并结合其它昆虫着色相关研究的成果进行分析比较,这对于今后系统开展昆虫着色机制及其演化的研究,以及对着色相关基因的利用,均具有重要参考价值。

关键词：家蚕着色模式突变机制基因调控代谢通路

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Flight Muscle and Wing Mechanical Properties Are Involved in Flightlessness of the Domestic Silkmoth,Bombyx mori

Flight Muscle and Wing Mechanical Properties Are Involved in...

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中国蚕学会2021年学术年会

作者： Kunpeng Lu Shubo Liang Minjin Han Chunman wu Jiangbo Song Chunlin Li songyuan wu Songzhen He Jianyu Ren Hai Hu Jianghong Shen Xiaoling Tong Fangyin Dai State Key Laboratory of Silkworm Genome Biology

Flight loss has occurred in many winged insect *** flightless silkmoth Bombyx mori,is domesticated from the wild silkmoth,Bombyx mandarina,which can *** this paper,we studied morphological characteristics attributed to flightlessness in *** domestic flightless *** strains and one *** population were used to compare morphological components of the flight apparatus,including wing characteristics（shape,forewing area,loading,and stiffness）,flight muscle（weight,ratio,and microscopic detail） and body *** with ***,*** strains have a larger body,greater wing loading,more flexible wings and a lower flight muscle *** arrangement in microscopy of dorsal longitudinal flight muscles（DLFMs） of *** was *** analysis of the sexes suggests that degeneration of flight muscles and reduction of wing mechanical properties（stiffness） are associated with silkmoth *** findings provide important clues for further research of the molecular mechanisms of *** flight loss.

关键词： Silkworm Flightlessness Domestication Morphology

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Comparative analysis of the integument transcriptomes of the black dilute mutant and the wild type silkworm Bombyx mori

Comparative analysis of the integument transcriptomes of the...

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中国蚕学会第九届青年学术研讨会

作者： songyuan wu Xiaoling Tong Chenxing Peng Gao Xiong Kunpeng Lu Hai hu Duan Tan Chunlin Li Minjin Han Cheng Lu Fangyin Dai State Key Laboratory of Silkworm Genome Biology Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural MinistrySouthwest University

The insect cuticle is a critical protective shell that is composed predominantly of chitin and various cuticular proteins and ***,insects often change their surface pigment patterns in response to selective pressures,such as threats from predators,sexual selection and environmental ***,the molecular mechanisms underlying the construction of the epidermis and its pigmentation patterns are not fully *** Lepidoptera,the silkworm is a favorable model for color pattern *** black dilute（bd） mutant of silkworm is the result of a spontaneous mutation;the larval body color is notably *** performed integument transcriptome sequencing of the wild-type strain Dazao and the mutant strains +/bd and bd/*** these experiments,during an early stage of the fourth molt,a stage at which approximately 51% of genes were expressed genome wide（RPKM ≥1） in each strain.A total of 254 novel transcripts were characterized using Cuffompare and BLAST *** of the transcriptome data revealed 28 diffrentially expressed genes（DEGs） that may contribute to bd larval melanism,including 15 cuticular protein genes that were remarkably highly expressed in the bd/bd *** suggest that these signifiantly upregulated cuticular proteins may promote melanism in silkworm larvae.

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Aspartate Decarboxylase is Required for a Normal Pupa Pigmentation Pattern in the Silkworm, Bombyx mori

Aspartate Decarboxylase is Required for a Normal Pupa Pigmen...

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第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会

作者： Fangyin Dai Liang Qiao Cun Cao Xiaofan Liu Xiaoling Tong Songzhen He Hai Hu Li Zhang songyuan wu Duan Tan Zhonghuai Xiang Cheng Lu State Key Laboratory of Silkworm Genome Biology College of Biotechnology Southwest University Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry Southwest University Institute of Entomology and Molecular Biology College of Life Sciences Chongqing Normal University

The pigmentation pattern of Lepidoptera varies greatly in different development stages. To date, the effects of key genes in the melanin metabolism pathway on larval and adult body color are distinct, yet the effects on pupal pigmentation remains unclear. In the silkworm, Bombyx mori, the black pupa(bp) mutant is only specifically melanized at the pupal stage. Using positional cloning, we found that a mutation in the Aspartate decarboxylase gene(BmADC) is causative in the bp mutant. In the bp mutant, a SINE-like transposon with a length of 493 bp was detected .2 kb upstream of the transcriptional start site of BmADC. This insertion causes a sharp reduction in BmADC transcript levels in bp mutants, leading to deficiency of β-alanine and N-β-alanyl dopamine(NBAD), but accumulation of dopamine. Following injection of β-alanine into bp mutants, the color pattern was reverted that of the wild-type silkworms. Additionally, melanic pupae resulting from knock-down of BmADC in the wild-type strain were obtained. These findings show that BmADC plays a crucial role in melanin metabolism and in the pigmentation pattern of the silkworm pupal stage. Finally, this study contributes to a better understanding of pupa pigmentation patterns in Lepidoptera.

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Molecular basis of the silkworm mutant rel causing red egg color and embryonic death

Molecular basis of the silkworm mutant rel causing red egg c...

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中国蚕学会2021年学术年会

作者： Jiangwen Luo Erxia An Yaru Lu Ling Yang Tingting Gai Songzhen He songyuan wu Hai Hu Chunlin Li Cheng Lu Xiaoling Tong Fangyin Dai State Key Laboratory of Silkworm Genome Biology Key Laboratory of Sericultural Biology and Genetic BreedingCollege of BiotechnologySouthwest University

The coloration and hatchability of insect eggs can affect individual and population ***,few genetic loci have been documented to affect both traits,and the genes involved in regulating these two traits are *** silkworm recessive mutant rel shows both red egg color and embryo *** studied the molecular basis of the rel phenotype *** genetic analysis,gene screening and sequencing,we found that two closely linked genes,BGIBMGA003497（Bm-re） and BGIBMGA003697（BmSema1a）,control egg color and embryo mortality,*** base pairs of the Bm-re gene are deleted in its open reading frame,and BmSema1a is expressed at abnormally low levels in mutant ***1a gene function verification was performed using RNAi and CRISPR/*** of the BmSema1a gene can cause the death of silkworm *** study revealed the molecular basis of silkworm rel mutant formation and indicated that the Sema1a gene is essential for insect embryo development.

关键词： BmSema1a CRISPR/Cas9 embryonic mortality red egg silkworm

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Body Shape and Coloration of Silkworm Larvae Are Influenced by a Novel Cuticular Protein

Body Shape and Coloration of Silkworm Larvae Are Influenced ...

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中国蚕学会第十届青年学术研讨会

作者： Gao Xiong Xiaoling Tong Tingting Gai Chunlin Li Liang Qiao Antónia Monteiro Hai Hu Minjin Han Xin Ding songyuan wu Zhonghuai Xiang Cheng Lu Fangyin Dai State Key Laboratory of Silkworm Genome Biology Southwest University Key Laboratory of Sericultural Biology and Genetic Breeding Ministry of AgricultureCollege of BiotechnologySouthwest University Institute of Entomology and Molecular Biology College of Life SciencesChongqing Normal University Department of Biological Sciences National University of Singapore Yale-NUS College

The genetic basis of body shape and coloration patterns on caterpillars is often assumed to be regulated separately,but it is possible that common molecules affect both types of trait *** we examine the genetic basis of a spontaneous cuticle defect in silkworm,where larvae exhibit a bamboo-like body shape and decreased *** performed linkage mapping and mutation screening to determine the gene product that affects body shape and coloration *** these mutant larvae we identified a null mutation in BmorCPH24,a gene encoding a cuticular protein with low complexity *** expression analyses showed that BmorCPH24 is expressed in the larval epidermis ***-mediated knockdown and CRISPR/Cas9-mediated knockout of BmorCPH24 produced the abnormal body shape and the inhibited pigment typical of the mutant *** addition,our results showed that BmorCPH24 may be involved in the synthesis of endocuticle and its disruption-induced apoptosis of epidermal cells that accompanied the reduced expression of R＆R-type larval cuticle protein genes and pigmentation gene Wnt1 Strikingly,BmorCPH24,a fast-evolving gene,has evolved a new function responsible for the assembly of silkworm larval cuticle and has evolved to be an indispensable factor maintaining the larval body shape and its coloration *** is the first study to identify a molecule whose pleiotropic function affects the development of body shape and color patterns in insect larvae.

关键词： apoptosis body shape color patterns cuticular protein novel gene

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