检索结果-南通市图书馆

Detection of Syndecan-1 and Heparanase-1 Genes in Esophageal Carcinoma by Quantitative RT-PCR

Clinical oncology and cancer researeh 2010年第4期7卷 253-258页

作者： Jun-li SI Yu-qin QI Jing-yuan CUI song-mei wang He wang mei LU Department of Gastroenterology Affiliated Qingdao Municipal Hospital of Qingdao University Medical College Qingdao 266011 Shan- dong Province China. Department of General Surgery Affiliated Qingdao Municipal Hospital of Qingdao University Medical College Qingdao 266011 Shandong Province China. Department of Hepatology Qingdao Infectious Diseases Hospital Qingdao 266033 Shandong Province China.

OBJECTIVE To quantitatively explore the expression of Syndecan-1 and heparanase-1 in esophageal cancer tissue as well as their relationship with the clinicopathological factors, in order to evaluate their roles in tumor invasion and *** Real-time fluorescence quantitative PCR （Q-PCR） was used to analyze the expression levels of Syndecan-1 and heparanase-1 genes￡？participants included 67 cases with esophageal cancers and 32 healthy *** The expression of Heparanase-1 gene in esophageal cancers was higher than that in normal esophageal tissue （P 〈 0.001）, and the expression of Syndecan-1 gene in the normal esophageal tissue was higher compared with esophageal cancers （P 〈 0.001）. The positive rates of Syndecan-1 and Heparanase-1 gene in esophageal cancer were 13.4% （9/67） and 85.1% （57/67）.The expression of Syndecan-1 and Heparanase-1 genes was signifi cantly related to di. erentiation, depth of infi ltration, lymph node metastasis, vessel metastasis, and TNM stages of disease （P 〈 0.05）. In an attempt to measure the association between the 2 agents, this study found that the expression of Syndecan-1 mRNA had a significantly negative correlation with the expression of Heparanase-1 mRNA by using Spearman rank correlation test （OR = -0.572, P 〈 0.001）.CONCLUSION Syndecan-1 and Heparanase-1 play important roles in the invasion and metastasis of esophageal cancer. The reduction of Syndecan-1 and/or the increase of Heparanase-1 may influence the invasion and metastasis of malignant *** the combination assay of Syndecan-1 and Heparanase-1 may contribute to the diagnosis and treatment of malignant tumors.

关键词： Syndecan-1 esophageal neoplasms neoplasm invasiveness neoplasm metastasis PCR

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Differentiation of smooth muscle progenitor cells in peripheral blood and its application in tissue engineered blood vessels

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第12期9卷 923-930页

作者： Shang-zhe XIE Ning-tao FANG Shui LIU Ping ZHOU Yi ZHANG song-mei wang Hong-yang GAO Luan-feng PAN Laboratory of Molecular Biology Shanghai Medical College Fudan University Shanghai 200032 China Key Laboratory of Molecular Engineering of Polymers Ministry of Education Department of Macromolecular Science Fudan University Shanghai 200433 China

Background： A major shortcoming in tissue engineered blood vessels （TEBVs） is the lack of healthy and easily attainable smooth muscle cells （SMCs）. Smooth muscle progenitor cells （SPCs）, especially from peripheral blood, may offer an alternative cell source for tissue engineering involving a less invasive harvesting technique. Methods： SPCs were isolated from 5-ml fresh rat peripheral blood by density-gradient centrifugation and cultured for 3 weeks in endothelial growth medium-2-MV （EGM-2-MV） medium containing platelet-derived growth factoroBB （PDGF BB）. Before seeded on the synthesized scaffold, SPC-derived smooth muscle outgrowth cell （SOC） phenotypes were assessed by immuno-fluorescent staining, Western blot analysis, and reverse transcription polymerase chain reaction （RT-PCR）. The cells were seeded onto the silk fibroin-modified poly（3-hydroxybutyrate-co-3-hydroxyhexanoate）（SF-PHBHHx） scaflblds by 6× 10^4 cells/cm^2 and cultured under the static condition for 3 weeks. The growth and proliferation of the seeded cells on the scaffold were analyzed by 3-（4,5-dimethylthiazol-2-yl）-diphenyltetrazolium bromide （MTT） assay, scanning electron microscope （SEM）, and 4,6-diamidino-2-phenylindole （DAPI） staining. Results： SOCs displayed specific ＂hill and valley＂ morphology, expressed the specific markers of the SMC lineage： smooth muscle （SM） a-actin, calponin and smooth muscle myosin heavy chain （SM MHC） at protein and messenger ribonucleic acid （mRNA） levels. RT-PCR results demonstrate that SOCs also expressed smooth muscle protein 22a （SM22a, a contractile protein, and extracellular matrix components elastin and matrix Gla protein （MGP）, as well as vascular endothelial growth factor （VEGF）. After seeded on the SF-PHBHHx scaffold, the cells showed excellent metabolic activity and proliferation. Conclusion： SPCs isolated from peripheral blood can be differentiated the SMCs in vitro and have an impressive growth potential in the biodegradable synthesized scaffold. Thus,

关键词： Smooth muscle progenitor cells (SPCs) Tissue-engineered blood vessels (TEBVs) Silk fibroin (SF) Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Hierarchical self-assembly of a collagen mimetic peptide （PKG）n（POG）2n（DOG）n via electrostatic interactions

引用

Frontiers of Computer Science 2011年第3期5卷 293-300页

作者： Ying SHI song wang Xiu-mei wang Qiang CAI Fu-Zhai CUI Heng-De LI Department of Materials Science and Engineering Tsinghua University Beijing 100084 China

A new type of collagen mimetic peptide, （PKG）n（POG）2n（DOG）n, with charged-domain ends had been designed and successfully prepared in this work, which self-assembled into collagen-like triple helices homotrimers. The collagen-like homo- trimers underwent higher level of self-assembly via static electrical interaction between positive and negative domains. Transmission electron microscope （TEM） examinations showed three typical morphologies of homotrimer assembly, which were defined as film, bicontinuous and fibril morphology in this paper. The film was formed in the initial stage and gradually transformed to bicontinuous or fibril morphology to improve stability of the assemblies or decrease surface energy. Furthermore, mechanism of assembly process was proposed based on TEM observations and theoretical analyses of packing equation.

关键词： collagen mimetic peptide assembly morphology

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Inhibitory effect of dimeric β peptide on the recurrence and metastasis of hepatocellular carcinoma in vitro and in mice

引用

World Journal of Gastroenterology 2008年第19期14卷 3054-3058页

作者： song-mei wang Jun Zhu Luan-Feng Pan Yin-Kun Liu Laboratory of Molecular Biology Shanghai Medical College Fudan University Shanghai 200032 China Department of Pharmacy Shanghai Chest Hospital Shanghai 200031 China Liver Cancer Institute Fudan University Shanghai 200032 China

AIM:To block the adhesion of tumor cells to the extra- cellular matrix, and prevent tumor metastasis and recur- rence, the dimer of the β peptide (DLYYLMDLSYSMKG- GDLYYLMDLSYSMK, β2) was designed and synthesized and its anti-adhesion and anti-invasion effects on hepa- tocellular carcinoma cells were assessed. Additionally, its influence on the metastasis and recurrence of mouse hepatocellular carcinoma was measured. METHODS:The anti-adhesion effect of β2 on the highly metastatic hepatocellular carcinoma cell line HCCLM6 cells and fibronectin (FN) was assayed by the MTT as- say. The inhibition of invasion of HCCLM6 cells by β2 was observed using a Transwell (modified Boyden chamber) and matrigel. Using the hepatocellular carcinoma metas- tasis model and LCI-D20 nude mice, the influence of β2 on the metastasis and recurrence of hepatocellular carci- noma after early resection was investigated. RESULTS:HCCLM6 cells co-incubated with 100 mmol/L, 50 mmol/L, 20 mmol/L or 10 mmol/L β2 for 3 h showed an obvious decrease in adhesion to FN. The adhesion inhibition ratios were 11.8%, 21.7%, 29.6% and 48.7%, respectively. Additionally, HCCLM6 cells cultured with 100 mmol/L β2 had a dramatic decrease in cell invasion. β2 was also observed to inhibit the incisal edge recur- rence and the distant metastasis of nude mice hepato- cellular carcinoma after early resection (P < 0.05). CONCLUSION:The β2 peptide can specifically block the adhesion and invasion of HCCLM6 cells, and can inhibit HCC recurrence and metastasis of LCI-D20 model pos-thepatectomy in vivo. Thus, β2 should be further studied as a new anti-tumor drug.

关键词： β peptide Hepatocellular carcinoma Anti-adhesion Invasion Metastasis Recurrence

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Constraints on holographic QCD phase transitions from PTA observations

引用

Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 71-78页

作者： song He Li Li Sai wang Shao-Jiang wang Center for Theoretical Physics and College of Physics Jilin University School of Physical Science and Technology Ningbo University Max Planck Institute for Gravitational Physics (Albert Einstein Institute) CAS Key Laboratory of Theoretical Physics Institute of Theoretical Physics Chinese Academy of Sciences School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of Sciences (UCAS) School of Physical Sciences University of Chinese Academy of Sciences (UCAS) Theoretical Physics Division Institute of High Energy Physics Chinese Academy of Sciences

The underlying physics of QCD phase transition in the early Universe remains largely unknown due to its strong-coupling nature during the quark-gluon plasma/hadron gas transition, yet a holographic model has been proposed to quantitatively fit the lattice QCD data while with its duration of the first-order phase transition(FoPT) left undetermined. At specific baryon chemical potential, the first-order QCD phase transition agrees with the observational constraint of baryon asymmetry. It, therefore, provides a scenario for phase transition gravitational waves(GWs) within the standard model of particle physics. If these background GWs could contribute dominantly to the recently claimed common-spectrum red noise from pulsar timing array(PTA) observations, the duration of this FoPT can be well constrained, and the associated primordial black holes are still allowed by current observations.

关键词： pulsar timing array QCD phase transition gravitational waves primordial black holes

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

MixerKT:A Knowledge Tracing Model Based on Pure MLP Architecture

引用

Computers, Materials & Continua 2025年第1期82卷 485-498页

作者： Jun wang Mingjie wang Zijie Li Ken Chen Jiatian mei Shu Zhang Key Laboratory of Education Informatization for Nationalities Ministry of EducationYunnan Normal UniversityKunming650000China Yunnan Key Laboratory of Smart Education Yunnan Normal UniversityKunming650000China

In the field of intelligent education,the integration of artificial intelligence,especially deep learning technologies,has garnered significant *** tracing(KT)plays a pivotal role in this field by predicting students’future performance through the analysis of historical interaction data,thereby assisting educators in evaluating knowledgemastery and tailoring instructional *** knowledge tracingmethods,largely based on Recurrent Neural Networks(RNNs)and Transformer models,primarily focus on capturing long-term interaction patterns in sequential ***,these models may neglect crucial short-term dynamics and other relevant *** paper introduces a novel approach to knowledge tracing by leveraging a pure Multilayer Perceptron(MLP)*** proposeMixerKT,a knowledge tracing model based on theHyperMixer framework,which uniquely integrates global and localMixer feature *** architecture enables more effective extraction of both long-terminteraction trends and recent learning behaviors,addressing limitations in currentmodels thatmay overlook these key *** evaluations on twowidely-used datasets,ASSIS Tments2009 and Algebra2005,demonstrate that MixerKT consistently outperforms several state-of-the-art models,including DKT,SAKT,and Separated Self-Attentive Neural Knowledge Tracing(SAINT).Specifically,MixerKT achieves higher prediction accuracy,highlighting its effectiveness in capturing the nuances of learners’knowledge *** results indicate that our model provides a more comprehensive representation of student learning patterns,enhancing the ability to predict future performance with greater precision.

关键词： Knowledge tracing multilayer perceptron channel mixer sequence mixer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synergistically enhanced ORR and HER performance on Co-N-C coupled in-situ generated PtCo intermetallic

引用

Journal of Energy Chemistry 2025年第1期100卷 721-729页

作者： Guanyu Luo Min song Lulu An Xiao Huang Qian Zhang Chenhao Zhang Tao Shen Shuang wang Deli wang Key Laboratory of Material Chemistry for Energy Conversion and Storage (Huazhong University of Science and Technology) Ministry of Education School of Chemistry and Chemical Engineering Huazhong University of Science and Technology

Integrating multi-scale sites in a composite catalyst is vital to realize efficient ***,a synergistic composite catalyst consisting of Co atomic sites and in-situ generated PtCo intermetallic compounds （IMCs）(o-PtCo@CoNC) is proposed through Co pre-anchoring and subsequent impregnation-reduction *** loading of Co atoms provides a chance for in-situ generating PtCo ordered intermetallic *** remaining Co single atoms and PtCo IMCs construct synergistic electrocatalytic *** from the ordered structure,synergistic effect of Pt Co IMCs and Co single atoms,o-PtCo@CoNC exhibits excellent electrocatalytic performance for oxygen reduction reaction （ORR） and hydrogen evolution reaction （HER） with mass activity of 1.21 A mgPt-1（at 0.9 V） and5.70 A mgPt-1（at an overpotential of 100 mV）,***,o-PtCo@CoNC delivers negligible loss of half-wave potential and overpotential during long-term stability test in acid solutions,with 13 mV decay after 50,000 potential cycles for ORR and a 2.7 mV decay after 20,000 potential cycles for *** integration strategy of single-atomic sites coupled IMCs paves the way for enhancing the activity and durability of Pt-based electrocatalysts.

关键词： Synergistic effect Intermetallics Single atomic sites Oxygen reduction reaction Hydrogen evolution reaction

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Electrochemical conversion of methane to bridge the gap in the artificial carbon cycle

引用

Journal of Energy Chemistry 2025年第1期100卷 286-308页

作者： Yuhao Peng Yuefeng song Ihar Razanau Juanxiu Xiao Wei Xiao Di Hu Guoxiong wang College of Chemistry and Molecular Sciences Hubei Key Laboratory of Electrochemical Power Sources Wuhan University State Key Laboratory of Catalysis Dalian National Laboratory for Clean Energy iChEM (Collaborative Innovation Center of Chemistry for Energy Materials) Dalian Institute of Chemical Physics Chinese Academy of Sciences Laboratory of Physical-Chemical Technologies SSPA Scientific and Practical Materials Research Centre of NAS of Belarus State Key Laboratory of Marine Resources Utilization in South China Sea Collaborative Innovation Center of Marine Science and Technology School of Marine Science and Engineering Hainan University Department of Chemical and Environmental Engineering University of Nottingham Ningbo China

Methane, an abundant one-carbon（C1） resource, is extensively used in the industrial production of vital fuels and value-added chemicals. However, current industrial methane conversion technologies are energy-and carbon-intensive, mainly due to the high activation energy required to break the inert C–H bond, low selectivity, and problematic side reactions, including CO2emissions and coke deposition. Electrochemical conversion of methane（ECM） using intermittent renewable energy offers an attractive solution, due to its modular reactor design and operational flexibility across a broad spectrum of temperatures and pressures. This review emphasizes conversion pathways of methane in various reaction systems, highlighting the significance and advantages of ECM in facilitating a sustainable artificial carbon cycle. This work provides a comprehensive overview of conventional methane activation mechanisms and delineates the complete pathways of methane conversion in electrolysis contexts. Based on surface/interface chemistry, this work systematically analyzes proposed reaction pathways and corresponding strategies to enhance ECM efficiency towards various target products, including syngas, hydrocarbons, oxygenates, and advanced carbon materials. The discussion also encompasses opportunities and challenges for the ECM process, including insights into ECM pathways, rational electrocatalyst design, establishment of benchmarking protocols, electrolyte engineering, enhancement of CH4conversion rates, and minimization of CO2emission.

关键词： Activation energy

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

All-optical neuromorphic devices: materials, working mechanism,and applications

引用

Science China Chemistry 2025年

作者： song wang Hao Chen Hui Huang College of Materials Science and Opto-Electronic Technology Center of Materials Science and Optoelectronics Engineering CAS Center for Excellence in Topological Quantum Computation CAS Key Laboratory of Vacuum Physics University of Chinese Academy of Sciences College of Chemistry and Molecular Engineering Peking University

Traditional von Neumann computing, with physical separation of memory and processing units, cannot satisfy the development of artificial intelligence and cloud computing. Neuromorphic computing, inspired by the human brain, has drawn much ***-optical neuromorphic (AON) devices employ optical signals as information carriers and leverage the neuromorphic functions to implement fast operation speed, low energy consumption, and high bandwidth of neuromorphic computing. Here, we discuss the recent progress in AON devices, including materials, device performance, working mechanisms, and applications. Moreover,the advantages and limitations of AON are presented and discussed. Finally, the perspective of AON devices points out the future research direction of neuromorphic computing.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

MoS_(2)Lubricate‑Toughened MXene/ANF Composites for Multifunctional Electromagnetic Interference Shielding

引用

Nano-Micro Letters 2025年第2期17卷 358-371页

作者： Jiaen wang Wei Ming Longfu Chen Tianliang song Moxi Yele Hao Zhang Long Yang Gegen Sarula Benliang Liang Luting Yan Guangsheng wang School of Physical Science and Engineering Beijing Jiaotong UniversityBeijing 100044People’s Republic of China Key Laboratory of Bio‑Inspired Smart Interfacial Science and Technology of Ministry of Education School of ChemistryBeihang UniversityBeijing 100191People’s Republic of China

The design and fabrication of high toughness electromagnetic interference(EMI)shielding composite films with diminished reflection are an imperative task to solve electromagnetic pollution *** MXene/ANF(aramid nanofibers)–MoS_(2)composite films with nacre-like layered structure here are fabricated after the introduction of MoS_(2)into binary MXene/ANF composite *** introduction of MoS_(2)fulfills an impressive“kill three birds with one stone”improvement effect:lubrication toughening mechanical performance,reduction in secondary reflection pollution of electromagnetic wave,and improvement in the performance of photothermal *** the introduction of MoS_(2)into binary MXene/ANF(mass ratio of 50:50),the strain to failure and tensile strength increase from 22.1±1.7%and 105.7±6.4 MPa and to 25.8±0.7%and 167.3±9.1 MPa,*** toughness elevates from 13.0±4.1 to 26.3±0.8 MJ m^(−3)(~102.3%)*** the reflection shielding effectiveness(SE_(R))of MXene/ANF(mass ratio of 50:50)decreases~10.8%.EMI shielding effectiveness(EMI SE)elevates to 41.0 dB(8.2–12.4 GHz);After the introduction of MoS_(2)into binary MXene/ANF(mass ratio of 60:40),the strain to failure increases from 18.3±1.9%to 28.1±0.7%(~53.5%),the SE_(R)decreases~22.2%,and the corresponding EMI SE is 43.9 *** MoS_(2)also leads to a more efficient photothermal conversion performance(~45 to~55℃).Additionally,MXene/ANF–MoS_(2)composite films exhibit excellent electric heating performance,quick temperature elevation(15 s),excellent cycle stability(2,2.5,and 3 V),and long-term stability(2520 s).Combining with excellent mechanical performance with high MXene content,electric heating performance,and photothermal conversion performance,EMI shielding ternary MXene/ANF–MoS_(2)composite films could be applied in many industrial *** work broadens how to achieve a balance between mechanical properties and versatility of composites in the case of high-function fillers.

关键词： MXene-MoS_(2) Lubrication toughening EMI shielding Photothermal conversion Electric heating performance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：