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Role of microRNA-25 in high glucose cultured Müller glia

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International Journal of Ophthalmology(English edition) 2021年第5期14卷 643-648页

作者： Yu Liu Han Shen song-tao yuan Qing-Huai Liu Department of Ophthalmology the First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029Jiangsu ProvinceChina

AIM:To investigate the role of microRNA-25(miR-25)in proliferation and apoptosis of retinal Müller glia(MG)under high glucose ***:The purity of the cultured cells was verified by immunocytochemistry and flow cytometry using antibodies that specifically recognized *** expression level of miR-25 under normal and high glucose conditions were validated by quantitative reverse transcription polymerase chain reaction(RT-q PCR).miR-25 mimics and negative control were transfected into MG and multiple functional experiments including cell counting kit-8 assay,EDU assay,and flow cytometry were conducted to explore the effects of miR-25 on the proliferation and apoptosis of high glucose cultured MG(HGMG).RESULTS:Immunocytochemistry and flow cytometry confirmed the high purity of primary cultured *** results showed that the expression level of miR-25 was significantly repressed in HGMG,while overexpression of miR-25 by miR-25 mimic markedly inhibited the high glucose induced cell apoptosis and promoted the proliferation of ***:The expression level of miR-25 is significantly downregulated in HGMG and its overexpression could attenuate the high glucose damages on MG by promoting proliferation and reducing apoptosis.

关键词： microRNA-25 Müller glia proliferation apoptosis

A novel xeno-free culture system for human retinal pigment epithelium cells

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International Journal of Ophthalmology(English edition) 2019年第4期12卷 563-570页

作者： Han Shen Min Wang Duo Li song-tao yuan Qing-Huai Liu Department of Ophthalmology the First Affiliated Hospital of Nanjing Medical University

AIM: To find out an animal-free, xeno-free culture method for human fetal retinal pigment epithelium(fRPE) cells aiming for cell-replacement therapy. METHODS: Human AB serum, knock-out serum replacement(KSR) and B27 were supplemented as a substitute of fetal bovine serum(FBS) in culture medium of human fRPE cells. Cell morphology was examined by light microscope and transmission electron microscope. Proliferation ability was detected by cell cycle analysis and examination of KI67 expression. Apoptosis was analyzed using FACS. The expression ofRPE-specific markers was demonstrated by quantitative real-time polymerase chain reaction(qPCR), Western blot(WB) and immunocytochemistry. Paracrine function was determined using enzyme-linked immunosorbent assay ***: Our results indicated that the optimum concentration of KSR was 15%, the optimum concentration of B27 was 2%, and the optimum concentration of human AB serum was 10%. fRPE cells cultured in 15% KSR and 2% B27 media showed repressed propagation and differentiation ability, and gradually lost epithelial morphology and RPE function. While fRPE cells cultured in 10% human AB serum media showed a typical cobblestone morphology with pigmentation, elevated proliferation ability, satisfying paracrine function and expressed RPE-specific markers. CONCLUSION: Our study indicates that culturing fRPE cells in 10% human AB serum-supplemented medium is more favorable compared with KSR, B27 and traditional FBS-supplemented mediums when fRPE cells are to be applied in cell-based therapy.

关键词： retinal pigment epithelium human AB serum cell-based therapy

Anthrax lethal toxin suppresses high glucose induced VEGF over secretion through a post-translational mechanism

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International Journal of Ophthalmology(English edition) 2015年第3期8卷 453-458页

作者： Wei-Wei Zhang Xin Wang Ping Xie song-tao yuan Qing-Huai Liu Department of Ophthalmology the First Affiliated Hospital of Nanjing Medical University Department of Endocrinology Jiangsu Province Hospital of TCM

AIM: To prove anthrax lethal toxin(Le Tx) blocks the mitogen activated protein kinases(MAPKs) activation by degrading the MAPK/ERK kinases(MEKs) to suppress vascular endothelial growth factor(VEGF) ***: Human adult retinal pigmented epithelium(ARPE) cells were cultured and treated with normal glucose, high glucose or high glucose with Le Tx for additional 24, 48 or 72 h for viable cell count. Total RNA from the ARPE was isolated for reverse transcription polymerase chain reaction(RT-PCR). The conditioned medium of ARPE cells treated in different group for 48 h was filtered and diluted to detect the concentration of VEGF by enzyme-linked immunosorbant *** the role of MEK/MAPK pathway in the secretion of VEGF by immunoblotting. RESULTS: In this study, we proved high glucose induced activation of the MAPK extracellular signal-regulated kinase(ERK1/2) and p38 in the ARPE cell line was blocked by anthrax Le Tx. Le Tx also inhibited high glucose induced ARPE cell over ***: Le Tx suppressed high glucose induced VEGF over secretion in the ARPE cells, mainly through a post-translational mechanism.

关键词： angiogenesis diabetic retinopathy retinal pigmented epithelium vascular endothelial growth factor anthrax lethal toxin

Phacoemulsification combined with transpupillary removal of silicone oil and intracapsular intraocular lens implantation

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International Journal of Ophthalmology(English edition) 2017年第11期10卷 1693-1697页

作者： Yu-Cheng Zhu Dong-Qing yuan Ping Xie Xiao-Yi Liu song-tao yuan Qing-Huai Liu Department of Ophthalmology the First Affiliated Hospitalwith Nanjing Medical University Nanjing 210029 JiangsuProvince China

AIM： To estimate the effectiveness of phacoemulsification and foldable intraocular lens（IOL） implantation combined with transpupillary silicone oil ***： There were 168 eyes of 168 candidate patients with cataract and silicone oil-filled eyes recruited in our study. All of the patients received the intraocular silicone oil removal surgery by transpupillary drainage and cataract extraction by phacoemulsification. Then the IOL implantation were also performed through corneal incision. RESULTS： The surgery was successfully completed in all eyes. Best corrected visual acuity（BCVA） and postoperative complications were recorded in three months after surgery. There were 143 eyes with BCVA improved, otherwise 25 eyes remained stable at the last follow-up visit. The mean BCVA statistically improved from 20/400±0.02 to 20/100±0.15（P〈0.001） and mean postoperative IOP was 13.85±2.18 mm Hg（P=0.415）. No intra-operative complications were ***： Phacoemulsification combined with transpupillary removal of silicone oil is a safe and simple effective method. In general, it enables quick recovery of visual acuity with less complication rate.

关键词： phacoemulsification posterior capsulorhexis silicone oil removal

Chordin-like 2 influences the differentiation fate of retinal pigment epithelium cells by dynamically regulating BMP pathway

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International Journal of Ophthalmology(English edition) 2022年第5期15卷 711-720页

作者： Duo Li song-tao yuan Xin-Yi Xie Han Shen Qing-Huai Liu Yong Yao Department of Ophthalmology the Affiliated Wuxi People’s Hospital of Nanjing Medical UniversityWuxiJiangsu ProvinceChina Department of Ophthalmology the First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu ProvinceChina

AIM:To explore the functions of Chordin-like 2,which is encoded by CHRDL2,in the process of retinal pigmented epithelium(RPE)differentiation and damage ***:The fetal RPE cells(f RPE)was obtained from aborted fetus which obeyed medical ***-time quantitative polymerase chain reaction was used to measure expression quantity of CHRDL2 and other functional genes *** down and overexpression was used to analyze the functions about Chordin-like ***-linked immunosorbent assay(ELISA)was used to detect the secretion of bone morphogenetic proteins 4(BMP4).Flow cytometry was used to analyze cell *** morphology was observed by phase contrast microscope(PCM).RESULTS:In normal RPE cells,CHRDL2 was firstly upregulated and followed a downregulation but eventually,it was expressed higher than the cells which undergone epithelial-mesenchymal transition(EMT).After knocking down CHRDL2,the secretion of BMP4 was decreased,RPErelated genes(OTX2,MITF,RPE65)were downregulated while EMT-related genes(SNAI1,VIM)were ***,the expression of these related genes after overexpression of CHRDL2 had contrary ***-like 2 also regulated the cell cycle by regulating BMP *** CHRDL2 was knocked down,more f RPE cells stayed in S phase of cell cycle,while adding BMP4 reduced the proportion of the cells in S ***,overexpression of CHRDL2 increased more BMP4 secretion,this effect decreased the number of cells in S phase,but exogenous BMP inhibitor also could change this *** last,in the process of RPE cells differentiation,adding BMP4 at early stage could intervene normal RPE *** with BMP4,inhibiting BMP pathway had no significant negative effect at early stage,but suppressed differentiation at late ***:BMP pathway can be activated in a correct temporal order,otherwise,the cells have incorrect differentiation *** Chordin-like 2 plays a role in dynamic regulation of BMP

关键词： retinal pigmented epithelium differentiation epithelial-mesenchymal transition BMP pathway cell proliferation

Evaluation of the safety and quality of day-case cataract surgery based on 4151 cases

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International Journal of Ophthalmology(English edition) 2019年第2期12卷 291-295页

作者： Min Zhuang Wen Fan Ping Xie song-tao yuan Qing-Huai Liu Chen Zhao Department of Ophthalmology the First Affiliated Hospital with Nanjing Medical University Department of Ophthalmology the Fourth Affiliated Hospital of Nantong University (Yancheng No.1 People’s Hospital) Eye Institute Eye & ENT Hospital Shanghai Medical College Fudan University Shanghai Key Laboratory of Visual Impairment and Restoration Key Laboratory of Myopia of State Health Ministry (Fudan University)

AIM: To evaluate the safety, quality and prospects of day-case cataract surgery performed in a Jiangsu public tertiary hospital METHODS: The general and clinical data for patients who underwent day-case cataract surgery between August 1, 2014 and December 31, 2016 at this hospital were collected. The incidences of intraoperative and postoperative complications, preoperative and postoperative bestcorrected visual acuities(BCVAs), delayed discharge rate, rate of unplanned re-admission to hospital, and patient satisfaction were analyzed. RESULTS: A total of 4151 patients received cataract phacoemulsification surgery to correct age-related, congenital, traumatic, or complicated cataracts. Of these, age-related cataracts were the most frequently occurring. Patient age ranged from 18 to 101 y and the vast majority of patients were between 60 and 80 years old. Of the 4151 patients, 64.73%(2687/4151) had a systemic disease. The number of patients increased over the years, with the average number of patients per month being 90.4, 124.83, and 183.42 in 2014, 2015 and 2016, respectively. The average preoperative BCVA was 0.102±0.057 and average postoperative BCVAs at 1 d, 1 wk, and 1 mo post surgery were 0.453±0.264, 0.657±0.285, and 0.734±0.244, respectively. For intraoperative complications, 4.12%(171/4151) had posterior capsule rupture, 0.79%(33/4151) had iris or ciliary body injury, and 0.048%(2/4151) had suprachoroidal hemorrhage. For postoperative complications, 4.38%(182/4151) had cornea edema, 7.78%(323/4151) had intraocular hypertension, 0.096%(4/4151) had IOL toxicity syndrome, 0.28%(12/4151) had retained lens cortex, and 0.048%(2/4151) had hyphema. The delayed discharge rate was 0.82%(44/4151) and the unplanned re-admission to the hospital was 0(0/4151). The patient satisfaction rate was 91.42%(3795/4151). CONCLUSION: Day-case cataract surgery is safe and effective with good prospects for development.

关键词： day-case cataract surgery medical safety medical quality complications re-admission rate satisfaction

Optic disc and peripapillary changes by optic coherence tomography in high myopia

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International Journal of Ophthalmology(English edition) 2018年第5期11卷 874-880页

作者： Ting Pan Yun Su song-tao yuan Hang-Cheng Lu Zi-Zhong Hu Qing-Huai Liu Department of Ophthalmology the First Affiliated Hospital of Nanjing Medical University

Myopia,a worldwide condition,is a multifactorial disease resulting in many ocular *** onset of myopia has a great tendency to develop high myopia and pathological myopia later in *** pathophysiology and progression of myopia is still *** to its involving in visual function,optic disc and peripapillary change in high myopia can＇t be neglected,and it may help in better understanding of the pathophysiology or mechanism of myopia ***,advanced imaging techniques have been developed,such as optical coherence tomography（OCT）,allowing for better detecting of optic disc and peripapillary *** is a highresolution and noninvasive measurement for detection of ocular ***,we provide an updated review of optic disc and peripapillary change in OCT image,including its characteristics and clinical *** also propose some problems needed further investigation.

关键词： high myopia optical coherence tomography optic disc peripapillary

Displacement of the retina after idiopathic macular hole surgery with different internal limiting membrane peeling patterns

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International Journal of Ophthalmology(English edition) 2021年第9期14卷 1408-1412页

作者： Jin Liu Zi-Zhong Hu Xin-Hua Zheng yuan-Long Li Jun-Long Huang Er-Bing Cao song-tao yuan Ping Xie Qing-Huai Liu Department of Ophthalmology the First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029Jiangsu ProvinceChina Department of Ophthalmology the Second Affiliated Hospital of Nanjing Medical UniversityNanjing 210029Jiangsu ProvinceChina Department of Ophthalmology Wuxi Children’s Hospitalthe Wuxi Affiliated Hospital of Nanjing Medical UniversityWuxi 214002Jiangsu ProvinceChina Department of Ophthalmology the Central Hospital of MaAnShanMaanshan 243031Anhui ProvinceChina Department of Ophthalmology the Affiliated Jiangning Hospital of Nanjing Medical UniversityNanjing 211100Jiangsu ProvinceChina

AIM:To explore retinal displacement after surgical treatment for idiopathic macular hole(IMH)with different internal limiting membrane(ILM)peeling ***:Totally 22 eyes from 20 patients with IMH were randomly allocated into two groups,N-T group(11 eyes)and T-N group(11 eyes).For patients in N-T group,ILM was peeled off from nasal to temporal *** patients in T-N group,ILM was peeled off from temporal to nasal ***,postoperative 1,3,and 6 mo,autofluorescence fundus images were collected for manual measurement of distances of fixed nasal(N),temporal(T),superior(S),and inferior(I)retinal points(bifurcation or crossing of retinal vessels)around the macula to the optic disc(OD).These were respectively defined as N-OD,T-OD,S-OD,and *** retinal displacement,macular holeclosure rate,and best corrected visual acuity(BCVA)were compared between the two groups after ***:At postoperative 1,3,and 6 mo,the macula slipped toward the OD,manifested by the decreased T-OD,N-OD,S-OD,and I-OD(P<0.05).No significant difference was found in the T-OD,N-OD,S-OD,and I-OD between N-T group and T-N *** closure rate was 100%both in N-T group and T-N *** was no significant difference in BCVA between two groups(P<0.05).CONCLUSION:The macula slips toward the OD after successful macular hole *** two different ILM peeling pattern show similar visual outcome and retinal displacement,which means ILM peeling directions are not the influencing factor of postoperative retinal displacement.

关键词： retinal displacement idiopathic macular hole internal limiting membrane peeling vitrectomy

Extracting intraocular foreign body at or near the ciliary body with scleral indentation in direct visualization

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International Journal of Ophthalmology(English edition) 2018年第3期11卷 533-535页

作者： Jiang-Dong Ji Yu-Zhi Ding Zi-Zhong Hu Ping Xie song-tao yuan Qing-Huai Liu Department of Ophthalmology the First Affiliated Hospital of Nanjing Medical University Department of Ophthalmology Zhongda Hospital Southeast University

Dear Editor,I＇m Dr. Qing-Huai Liu from Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical University,Nanjing, China. I write to present four cases diagnosed with the intraocular foreign body （IOFB） at or near the ciliary body and to evaluate the effect of extracting IOFB with scleral indentation in direct visualization.

关键词： Extracting intraocular foreign body at or near the ciliary body scleral indentation direct visualization