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海南木榄叶的蛋白鉴定及在Pb^(2+)、Cd^(2+)胁迫下差异蛋白的分析

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分子植物育种 2024年第5期22卷 1454-1470页

作者：宋媛陈光英刘红赵振东何文英海南师范大学热带药用资源化学教育部重点实验室海口571127 海南师范大学海口市热带特色药食同源植物研究与开发重点实验室海口571127 海南大学分析测试中心海口570228

木榄(Bruguiera gymnorhiza(L.)Lam.)是海南红树药用植物之一,为深入研究开发木榄叶的价值和功能,采用优化条件下BPP+酚抽提法的双向电泳技术,获得海南木榄叶的蛋白指纹图谱,并筛选出高表达蛋白;利用基质辅助激光解析电离飞行时间质谱(M... 详细信息

木榄(Bruguiera gymnorhiza(L.)Lam.)是海南红树药用植物之一,为深入研究开发木榄叶的价值和功能,采用优化条件下BPP+酚抽提法的双向电泳技术,获得海南木榄叶的蛋白指纹图谱,并筛选出高表达蛋白;利用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)鉴定高表达蛋白的酶水解产物及确定蛋白名称,并进行初步功能分析;另外,对重金属Pd^(2+)和Cd^(2+)胁迫木榄叶条件下的差异蛋白进行分离提取及鉴定。结果表明,海南木榄叶有60个高表达的蛋白,包括差向异构酶、DNA连接酶等42个蛋白,涉及木榄叶生长、发育、遗传及植物抗逆等多种功能;重金属Pd^(2+)和Cd^(2+)胁迫的差异蛋白分别有4个和6个,与植物生长、遗传及高效光合作用等植物功能有关。本研究不仅为进一步开发利用海南产木榄叶中的植物蛋白提供理论依据,也对发现木榄叶与重金属Pd^(2+)、Cd^(2+)胁迫相关的关键蛋白、理解海南岛的生态系统服务和生态效益具有一定的理论和实际意义。

关键词：木榄叶蛋白图谱 Pd^(2+) Cd^(2+) 差异蛋白

Upregulation of miR-143-3p attenuates oxidative stress-mediated cell ferroptosis in cardiomyocytes with atrial fibrillation by degrading glutamic-oxaloacetic transaminase 1

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BIOCELL 2021年第3期45卷 733-744页

作者： yuan song CAI WEI JINGJING WANG Department of Functional Examination The Sixth Affiliated Hospital of Xinjiang Medical UniversityUrumqi830000China Health Care Third Department Xinjiang Military Region General HospitalUrumqi830000China Department of Emergency The People’s Hospital of Suzhou New DistrictSuzhou215000China

Oxidative stress-mediated cell death in cardiomyocytes contributes to the development of atrial ***,the detailed mechanisms are still *** the present study,we established atrial fibrillation models in *** cardiomyocytes were isolated from atrial fibrillation mice and normal mice and were cultured in vitro,*** results showed that cell proliferation and viability in cardiomyocytes with atrial fibrillation were significantly lower than the cells from the normal ***,atrial fibrillation cardiomyocytes were prone to suffer from apoptotic cell ***,the oxidative stress and ferroptosis-associated signatures were significantly increased in atrial fibrillation cardiomyocytes compared to normal cardiomyocytes,and ferroptosis inhibitor and NAC rescued cell viability in atrial fibrillation cardiomyocytes during in vitro cell *** addition,low-expressed miR-143-3p was observed in atrial fibrillation cardiomyocytes compared to normal cardiomyocytes,and overexpression of miR-143-3p increased cell proliferation and inhibited cell death in atrial fibrillation ***,glutamic-oxaloacetic transaminase 1 could be negatively regulated by miR-143-3p in normal cardiomyocytes,and miR-143-3p overexpression inhibited cell ferroptosis in atrial fibrillation cardiomyocytes by sponging glutamic-oxaloacetic transaminase ***,overexpression of miR-143-3p increased cell viability and promoted cell proliferation in cardiomyocytes with atrial fibrillation by inhibiting glutamic-oxaloacetic transaminase 1 mediated oxidative damages and cell ferroptosis.

关键词： Atrial fibrillation Ferroptosis Cardiomyocytes MiR-143-3p Glutamic-oxaloacetic transaminase 1 Ferrostatin-1

Morphological Studies on Four Brackish Water Ciliates of the Class Spirotrichea(Protista, Ciliophora)

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Journal of Ocean University of China 2019年第3期18卷 663-674页

作者： song yuan LIU Yongqiang PAN Bo LUO Xiaotian song Wen WARREN Alan College of Marine Life Sciences Ocean University of China Qingdao 266003 China Institute of Evolution and Marine Biodiversity Ocean University of China Qingdao 266003 China Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Department of Life Sciences Natural History Museum Cromwell Road London SW7 5BD UK

We reinvestigate the morphology of two oligotrich and two hypotrich ciliates collected from the coasts of north and south China, viz., Parallelostrombidium obesum Liu et al., 2015, Spirostrombidium apourceolare Liu et al., 2013, Protogastrostyla pulchra(Pereyaslawzewa, 1886) Gong et al., 2007, and Uncinata bradburyae(Gong et al., 2001) Luo et al., 2015. All the populations match well with the original samples identified in previous studies. Supplemental taxonomic data were supplied for these four species of the new populations. The species Parallelostrombidium obesum is characterised by its dorsal-ventrally flattened body shape with anterior and posterior ends transversely truncating, as well as two thigmotactic membranelles and girdle kinety that spirals around the cell one and a half times. Spirostrombidium apourceolare is characterised by its elongate ellipsoidal and dorsal-ventrally flattened body shape, two thigmotactic membranelles, about 11-27 ovoid macronuclear nodules, and girdle kinety that spirals around the cell twice with two undulations. Protogastrostyla pulchra is characterised by its elongate body shape and unique, caudally located food vacuole. Based on the new populations, we described the smaller cortical granules, clustered around dorsal bristles for the first time. The new population of Uncinata bradburyae shares the diagnostic features with the type population, which include prominent beak-like projection in anterior region, the conspicuous gap of adoral zone, proximalmost adoral membranelles distinctly elongated, and infraciliature.

关键词： hypotrichs ciliates oligotrichs Parallelostrombidium Protogastrostyla Spirostrombidium taxonomy Uncinata

Investigation and assessment on mountain tunnels and geotechnical damage after the Wenchuan earthquake

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Science China(Technological Sciences) 2009年第2期52卷 546-558页

作者： WANG ZhengZheng GAO Bo JIANG yuanJun yuan song School of Civil Engineering Southwest Jiaotong UniversityChengdu 610031China Department of Civil Engineering University of TokyoTokyo 1138656Japan

On May 12, 2008, a strong earthquake with a magnitude of 8.0 (Ms) struck Wenchuan town, in the eastern Sichuan area of west China. Following the earthquake on May 18, the Southwest Jiaotong University organized a damage survey team and dispatched it to the affected area for the investigation into the damage and collection of information and data. This paper outlines the findings of this investigation on the earthquake disaster to mountain tunnels and geotechnical engineering. The systematic investigation, involving geological conditions, design documents, construction and maintenance records of the tunnels, has been conducted and the degree of damage to investigated tunnels has been assessed according to the width and length of cracks, the stability of the slope above the tunnel, and the condition of the groundwater inrush. The results show that the major damage of the mountain tunnels was mainly concentrated in the tunnel portals due to widespread landslides and rockfalls, and the inner part of investigated tunnels suffered moderate damages mainly due to fault displacements. It is hoped that the information shared herein could enhance the outstanding of seismic behavior of mountain tunnels and improve seismic design and construction procedures.

关键词： damage investigation wenchuan earthquake mountain tunnels ground failures

一种含1,2,3-三氮唑席夫碱的Al^(3+)荧光探针及其应用

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分析试验室 2024年第5期43卷 660-668页

作者：廖元淏王帅冯华杰陈光英宋媛吴禄勇何文英热带药用资源化学教育部重点实验室海南师范大学化学与化工学院海口571158

本文设计合成了5-苯基-2-苯基-1,2,3-三唑-1-4-酰肼(PP),将其与水杨醛反应,构建了一种席夫碱荧光探针(PPS)。荧光光谱及紫外光谱测定表明,PPS能够高选择性和强抗干扰性地检测乙醇溶液中的Al^(3+),检出限为8.28 nmol/L;在紫外光下可发生... 详细信息

本文设计合成了5-苯基-2-苯基-1,2,3-三唑-1-4-酰肼(PP),将其与水杨醛反应,构建了一种席夫碱荧光探针(PPS)。荧光光谱及紫外光谱测定表明,PPS能够高选择性和强抗干扰性地检测乙醇溶液中的Al^(3+),检出限为8.28 nmol/L;在紫外光下可发生明显的溶剂变色效应,在含水量大于60%的有机溶剂中存在聚集诱导发光(AIE)效应,质谱、工作曲线、核磁滴定及理论计算结果均证明,PPS和Al^(3+)可以络合比1∶1进行反应,生成平面结构的化合物。该探针具有低的细胞毒性且成功用于HeLa细胞的显色成像,有望被开发利用为具有AIE聚集效应的发光材料及生物体内测定Al^(3+)的荧光探针。

关键词： 5-苯基-2-苯基-1,2,3-三唑-1-4-酰肼席夫碱 Al^(3+) 识别机理细胞成像

Effect of Carbon Content on Mechanoreduction of Copper Oxide

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Chinese Physics Letters 1999年第8期16卷 591-593页

作者： LIU Lin yuan song-liu DONG yuan-da CUI Kun The State Key Laboratory of Plastic Forming Stimulation and Die&Mould Technology Huazhong University of Science&TechnologyWuhan 430074 Department of Physics Huazhong University of Science&TechnologyWuhan 430074 Institute of Materials Shanghai UniversityShanghai 200072

The reduction of CuO with different amount of carbon(CuO:C=2:1,2:1.5,and 2:2,in molar ratio)induced by ball milling is examined by x-ray diffraction and transmission electron *** is found that reduction process is closely related to the carbon *** can be completely converted into Cu if an excessive C is used(i.e.,C=1.5 or 2.0mol).However,the reduction of CuO follows a two-step process,CuO→Cu→Cu2O,if the mixture only contains a nominal C content(i.e.,C=1 mol).A tentative model in terms of solid state reaction at the interfaces is proposed to explain the effect of carbon content on the mechanoreduction of CuO.

关键词： carbon nominal content

Unveiling the molecule–plasmon interactions in surface-enhanced infrared absorption spectroscopy

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National Science Review 2020年第7期7卷 1228-1238页

作者： Jun Yi En-Ming You song-yuan Ding Zhong-Qun Tian State Key Laboratory of Physical Chemistry of Solid Surfaces (PCOSS) Collaborative Innovation Centre of Chemistry for Energy Materials (iChEM) and Department of Chemistry College of Chemistry and Chemical Engineering Xiamen University NSF Nanoscale Science and Engineering Center University of California

Nanostructure-based surface-enhanced infrared absorption(SEIRA) spectroscopy has attracted tremendous interest as an ultrasensitive detection tool that supplies chemical-fingerprint information. The interactions between molecular vibrations and plasmons lead to not only the enhancement of spectral intensity, but also the distortion of spectral Lorentzian lineshapes into asymmetric Fano-type or more complicated lineshapes in the SEIRA spectra; this effect hampers the correct readout of vibrational frequencies and intensities for an accurate interpretation of the measured spectra and quantitative *** this work, we investigate the Fano interference between molecular vibrations and plasmons based on exact electrodynamic simulations and theoretical models. We report that, even if the molecular vibrational energy is equal to the plasmon resonant energy, the molecule–nanostructure distance-dependent dipole–dipole interactions, the plasmon-mediated coherent intermolecular interactions and the decay rates of plasmons have a significant impact on the SEIRA lineshapes. This study paves the way for controllable Fano interference at the nanoscale and more studies on plasmon-dressed molecular electronic or vibrational excited states.

关键词： surface plasmons surface-enhanced infrared absorption Fano resonance coupling strength spectral lineshape

不同含水量及冻结温度对黑土冻融循环过程有机碳矿化的影响

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水土保持学报 2024年第4期38卷 55-62页

作者：张博刘会敏毕鑫宇高航宋媛胡亚鲜栗现文西北农林科技大学水利与建筑工程学院陕西杨凌712100 西北农林科技大学旱区农业水土工程教育部重点实验室陕西杨凌712100 西北农林科技大学水土保持科学与工程学院黄土高原土壤侵蚀与旱地农业国家重点实验室陕西杨凌712100

[目的]冻融过程土壤呼吸在年土壤呼吸总量中占有重要比例,研究探讨土壤冻融过程中含水量、冻结温度和冻融循环次数对土壤碳矿化动态的影响。[方法]以黑龙江省嫩江县鹤山农场九三水土实验站黑土为研究对象,开展室内冻融程度模拟试验,进行... 详细信息

[目的]冻融过程土壤呼吸在年土壤呼吸总量中占有重要比例,研究探讨土壤冻融过程中含水量、冻结温度和冻融循环次数对土壤碳矿化动态的影响。[方法]以黑龙江省嫩江县鹤山农场九三水土实验站黑土为研究对象,开展室内冻融程度模拟试验,进行7次冻融循环,设置100%田间持水量(100%WHC)、60%田间持水量(60%WHC)和30%田间持水量(30%WHC)3种土壤含水量;10℃恒温处理(对照)、-5℃冻结处理(轻度冻结)和-15℃冻结处理(重度冻结)3种环境温度。[结果]冻融循环次数、含水量和冻结温度对CO_(2)排放量有显著影响,影响度分别为-0.63,0.21,0.14。解冻过程显著增加土壤碳矿化量;轻度冻结时,前3次冻融循环60%WHC土壤碳矿化量比100%WHC和30%WHC分别提高33.0%,35.2%,后4次冻融循环差异不明显;重度冻结时,前2次冻融循环100%WHC土壤碳矿化量,比60%WHC和30%WHC土壤分别提高25.2%,68.0%,后5次冻融循环差异不明显。[结论]冻融循环次数对土壤CO_(2)排放量影响最大,含水量次之,冻结温度最小。冻融作用增加低含水量土壤的CO_(2)累积排放量;降低高含水量土壤的CO_(2)累积排放量;而对中等含水量土壤,轻度冻结增加CO_(2)累积排放量,重度冻结降低CO_(2)累积排放量。一级动力学方程对冻融土壤CO_(2)排放量的拟合效果较好(R^(2)>0.997),含水量和冻结温度对有机碳矿化潜力C0值有显著影响。

关键词：土壤有机碳矿化土壤含水量冻融循环冻结温度黑土

彩色多普勒超声检查对乳腺良恶性病变的鉴别诊断价值

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癌症进展 2024年第3期22卷 327-330页

作者：宋媛魏世荣杨晓宇陕西省康复医院超声科西安710065 宝鸡市妇幼保健院超声医学科陕西宝鸡7210000

目的探讨彩色多普勒超声检查对乳腺良恶性病变的鉴别诊断价值。方法选取74例疑似乳腺癌患者,均进行钼靶X线检查与彩色多普勒超声检查,比较钼靶X线检查与彩色多普勒超声检查的影像学特征。以病理检查结果为金标准,分析钼靶X线检查与彩色... 详细信息

目的探讨彩色多普勒超声检查对乳腺良恶性病变的鉴别诊断价值。方法选取74例疑似乳腺癌患者,均进行钼靶X线检查与彩色多普勒超声检查,比较钼靶X线检查与彩色多普勒超声检查的影像学特征。以病理检查结果为金标准,分析钼靶X线检查与彩色多普勒超声检查对乳腺良恶性病变的鉴别诊断价值。结果病理检查结果显示,乳腺癌52例,乳腺良性病变22例。彩色多普勒超声检查诊断乳腺癌的结果与病理检查结果的一致性(Kappa=0.840)高于钼靶X线检查(Kappa=0.580);彩色多普勒超声检查诊断乳腺癌的准确度、特异度、灵敏度、阳性预测值、阴性预测值均高于钼靶X线检查。结论彩色多普勒超声检查对乳腺良恶性病变的鉴别诊断价值较高,可清晰反映各类病灶的影像学特征,值得临床推广应用。

关键词：乳腺癌钼靶X线检查彩色多普勒超声检查鉴别诊断