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肺癌患者外周血和胸腔积液T细胞受体组库特征分析

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中国临床医学 2024年第1期31卷 60-67页

作者：宋娟王林林胡洁宋元林童琳复旦大学附属中山医院呼吸与危重症医学科上海200032 上海市重大传染病与生物安全研究院上海200032

目的比较肺癌患者外周血和胸腔积液的T细胞受体(TCR)组库的免疫学特征。方法收集2020年7月至2021年1月在复旦大学附属中山医院就诊的5例合并恶性胸腔积液肺癌患者的外周血和胸腔积液,分离外周血单个核细胞(PBMC)和胸腔积液单个核细胞(... 详细信息

目的比较肺癌患者外周血和胸腔积液的T细胞受体(TCR)组库的免疫学特征。方法收集2020年7月至2021年1月在复旦大学附属中山医院就诊的5例合并恶性胸腔积液肺癌患者的外周血和胸腔积液,分离外周血单个核细胞(PBMC)和胸腔积液单个核细胞(PEMC),提取基因组DNA,采用Illumina MiSeq平台对TCR进行高通量测序,分析TCR组库的多样性等特征。结果 PBMC和PEMC中的TCR克隆型、VJ pairs和互补决定区3(CDR3)序列的多样性和相关性差异均无统计学意义。PBMC中检测到的TCR克隆数量高于PEMC(P<0.05),VJ pairs和CDR3数量与PEMC差异无统计学意义。PBMC和PEMC配对样本的共有VJ pairs数量占总VJ pairs的比例高于共有克隆型和CDR3序列的数量比例(P<0.05),后两者差异无统计学意义。PEMC中高扩增克隆(HEC)数量和占比均高于PBMC。同时鉴定了36个在PBMC和PEMC中差异表达的VJ pairs,其中25个在PEMC中表达升高、9个在PEMC中表达降低。结论肺癌患者外周血和胸腔积液的TCR组库表现出不同的特征性变化,可能为肺癌重要的免疫病理学特征。

关键词：肺癌恶性胸腔积液 T细胞受体组库外周血单个核细胞胸腔积液单个核细胞多样性

Xuebijing alleviates LPS-induced acute lung injury by downregulating pro-inflammatory cytokine production and inhibiting gasdermin-E-mediated pyroptosis of alveolar epithelial cells

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中国天然药物:英文版 2023年第8期21卷 576-588页

作者： ZHANG Cuiping CHEN Xiaoyan WEI Tianchang song Juan TANG Xinjun BI Jing CHEN Cuicui ZHOU Jian SU Xiao song yuanlin Shanghai Key Laboratory of Lung Inflammation and Injury Department of Pulmonary MedicineZhongshan HospitalFudan UniversityShanghai 200032China The Unit of Respiratory Infection and Immunity Institut Pasteur of ShanghaiChinese Academy of SciencesShanghai 200031China Shanghai Institute of Infectious Disease and Biosecurity Shanghai 200032China Shanghai Respiratory Research Institute Shanghai 200032China National Clinical Research Center for Aging and Medicine Huashan HospitalFudan UniversityShanghai 200032China Department of Pulmonary Medicine Jinshan Hospital of Fudan UniversityShanghai 201508China

Acute lung injury/acute respiratory distress syndrome(ALI/ARDS)is characterized by diffuse alveolar injury primarily caused by an excessive inflammatory ***,the lack of effective pharmacotherapy currently available contributes to the high mortality rate in patients with this ***(XBJ),a traditional Chinese medicine recognized for its potent anti-inflammatory properties,exhibits promise as a potential therapeutic agent for ALI/*** study aimed to explore the preventive effects of XBJ on ALI and its underlying *** this end,we established an LPS-induced ALI model and treated ALI mice with *** results demonstrated that pre-treatment with XBJ significantly alleviated lung inflammation and increased the survival rate of ALI mice by 37.5%.Moreover,XBJ substantially suppressed the production of TNF-α,IL-6,and IL-1βin the lung ***,we performed a network pharmacology analysis and identified identified 109 potential target genes of XBJ that were mainly involved in multiple signaling pathways related to programmed cell death and anti-inflammatory ***,we found that XBJ exerted its inhibitory effect on gasdermin-E-mediated pyroptosis of lung cells by suppressing TNF-α***,this study not only establishes the preventive efficacy of XBJ in ALI but also reveals its role in protecting alveolar epithelial cells against gasdermin-E-mediated pyroptosis by reducing TNF-αrelease.

关键词： Pyroptosis Gasdermin-E TNF-α Xuebijing Acute lung injury

Molecular mechanisms and roles of pyroptosis in acute lung injury

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Chinese Medical Journal 2022年第20期135卷 2417-2426页

作者： Tianchang Wei Cuiping Zhang yuanlin song Department of Pulmonary Medicine Shanghai Key Laboratory of Lung Inflammation and InjuryZhongshan HospitalFudan UniversityShanghai 200032China Shanghai Institute of Infectious Disease and Biosecurity Shanghai 200032China Shanghai Respiratory Research Institute Shanghai 200032China Jinshan Hospital of Fudan University Shanghai 201508China

Acute lung injury(ALI)and acute respiratory distress syndrome(ARDS),which are characterized by excessive inflammation and accompanied by diffuse injury of alveoli,can result in severe respiratory *** morbidity and mortality of patients remain high because the major treatments for ALI/ARDS are mainly supportive due to the lack of effective *** studies have demonstrated that the aggravation of coronavirus disease 2019(COVID-19)leads to severe pneumonia and even ***,a biological process identified as a type of programed cell death,is mainly triggered by inflammatory caspase activation and is directly meditated by the gasdermin protein family,as well as being associated with the secretion and release of pro-inflammatory *** and experimental evidence corroborates that pyroptosis of various cells in the lung,such as immune cells and structural cells,may play an important role in the pathogenesis of“cytokine storms”in ALI/ARDS,including those induced by ***,with a focus on ALI/ARDS and COVID-19,we summarized the recent advances in this field and proposed the theory of an inflammatory cascade in pyroptosis to identify new targets and pave the way for new approaches to treat these diseases.

关键词： Acute lung injury Acute respiratory distress syndrome Caspase Coronavirus disease 2019(COVID-19) Gasdermin Inflammasome Pyroptosis

Phenotype and treatment of chronic obstructive pulmonary disease

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Chinese Medical Journal 2014年第17期127卷 3041-3042页

作者： song yuanlin Wang Chen Department of Pulmonary Medicine Zhongshan Hospital Fudan University Shanghai 200032 China Department of Pulmonary and Critical Care Medicine Beijing Hospital Beijing 100730 China

Chronic obstructive pulmonary disease （COPD） is the most common chronic airway disease affecting Chinese population and it ranks number tour leading cause of death. The high mortality and morbidity, increased prevalence, significant loss of labor, and high medical expenditure warrants attention from the health organization and society to standardize the diagnosis and treatment of COPD. Overall COPD treatment efficacy has been significantly improved with application of long activating bronchial dilator （LABD）, due to the imbalance and geographically different distribution of medical resource and education, standardized treatment under COPD guideline remain the major protocol in a long time period in the future.

关键词： chronic obstructive pulmonary disease phenotype treatment

COVID-19-associated gastrointestinal and liver injury:clinical features and potential mechanisms

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Signal Transduction and Targeted Therapy 2020年第1期5卷 308-315页

作者： Peijie Zhong Jing Xu Dong Yang Yue Shen Lu Wang Yun Feng Chunling Du yuanlin song Chaomin Wu Xianglin Hu Yangbai Sun Department of Musculoskeletal Surgery Fudan University Shanghai Cancer Center200032 ShanghaiChina Department of Oncology Shanghai Medical CollegeFudan University200032 ShanghaiChina Department of Gastroenterology and Hepatology Huaihe Hospital of Henan University475000 KaifengChina Clinical Medical College Southwest Medical University646000 LuzhouChina Department of Endocrinology and Metabolism Zhongshan HospitalFudan University200032 ShanghaiChina Department of Pulmonary and Critical Care Medicine Zhongshan HospitalFudan University200032 ShanghaiChina Department of Gastroenterology and Hepatology Zhongshan HospitalFudan University200032 ShanghaiChina Department of Gastroenterology and Hepatology Shanghai General HospitalShanghai Jiao Tong University School of Medicine200080 ShanghaiChina Department of Pulmonary and Critical Care Medicine QingPu Branch of Zhongshan Hospital Affiliated to Fudan University201700 ShanghaiChina Department of Surgery Training Base Fudan University Shanghai Cancer Center200032 ShanghaiChina

Coronavirus disease-2019(COVID-19)is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The infection is spreading globally and poses a huge threat to human *** common respiratory symptoms,some patients with COVID-19 experience gastrointestinal symptoms,such as diarrhea,nausea,vomiting,and loss of ***-CoV-2 might infect the gastrointestinal tract through its viral receptor angiotensin-converting enzyme 2(ACE2)and there is increasing evidence of a possible fecal–oral transmission *** addition,there exist multiple abnormalities in liver ***-19-related liver injury may be due to drug-induced liver injury,systemic inflammatory reaction,and hypoxia–ischemia reperfusion *** direct toxic attack of SARS-CoV-2 on the liver is still *** review highlights the manifestations and potential mechanisms of gastrointestinal and hepatic injuries in COVID-19 to raise awareness of digestive system injury in COVID-19.

关键词： gastrointestinal injuries respiratory

胸部定量CT在慢性阻塞性肺疾病早期诊断中的应用价值

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中国临床医学 2024年第2期31卷 208-214页

作者：黄锦贤侯东妮谢丛意陈淑靖许诺周亚南姜红妮宋元林复旦大学附属中山医院厦门医院呼吸科厦门361015 复旦大学附属中山医院呼吸科上海200032

目的探讨胸部定量CT在早期诊断慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)中的应用价值。方法回顾性分析2013年9月20日至2014年5月20日上海市某社区COPD高危人群受访者,以及复旦大学附属中山医院门诊的COPD高危咨... 详细信息

目的探讨胸部定量CT在早期诊断慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)中的应用价值。方法回顾性分析2013年9月20日至2014年5月20日上海市某社区COPD高危人群受访者,以及复旦大学附属中山医院门诊的COPD高危咨询者共138例的临床资料。所有高危者在基线及1年后均行肺功能及胸部CT检查,将胸部CT图像导入定量CT分析软件,收集定量CT数据。根据1年后的随访肺功能结果分为COPD组(n=40)与非COPD组(n=98),比较2组在基线时肺功能、定量CT指标的差别。采用二元logistic回归分析COPD高危者随访1年后发生COPD的预测指标,通过ROC曲线评估logistic回归模型的效能。结果2组基线时性别、体质量指数(BMI)、第1秒用力呼气的容积占预计值的百分比(FEV1%pred)、气道壁面积比值(WA%)、气道总数(TAC)、气道壁厚度(WT)差异无统计学意义。COPD组的气道内周长为10 mm处气管壁面积的平方根[Pi10;3.43(3.30,3.54)vs 3.23(3.15,3.36),P<0.001]、低于﹣950 HU的低衰减区面积百分比(LAA%-950)[2.06(0.32,6.29)vs 0.57(0.25,1.89),P=0.015]均显著高于非COPD组,COPD组平均肺密度(MLD)低于非COPD组[(﹣799.89±35.62)vs(﹣783.60±43.52),P=0.038]。二元logistic回归分析显示,年龄和Pi10是患COPD的影响因素(P<0.05),ROC曲线下面积为0.791(95%CI 0.714~0.868)。结论在肺功能正常的COPD高危人群中,Pi10、LAA%﹣950升高的患者1年后COPD发病率高,提示胸部定量CT的指标如Pi10、LAA%-950有助于临床医生识别早期COPD。

关键词：慢性阻塞性肺疾病胸部定量CT 肺功能气道壁厚度肺密度

噻托溴铵能倍乐?和噻托溴铵吸乐?在慢性阻塞性肺疾病患者中满意度和偏好度研究

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临床肺科杂志 2024年第1期29卷 18-23页

作者：时国朝林英翔郭禹标杨汀郑则广王玮王刚宋元林陈荣昌上海交通大学医学院附属瑞金医院呼吸与危重症医学科上海200025 首都医科大学附属北京朝阳医院呼吸与危重症医学科北京100020 中山大学附属第一医院呼吸与危重症医学科广东广州510080 中日友好医院呼吸与危重症医学科北京100029 广州医科大学附属第一医院呼吸内科广州呼吸疾病研究院广东广州510120 中国医科大学附属第一医院呼吸内科辽宁沈阳110001 四川大学华西医院呼吸与危重症医学科四川成都610041 复旦大学附属中山医院呼吸内科上海200032 深圳市人民医院呼吸与危重症医学科深圳市呼吸疾病研究所广东深圳518001

目的比较中国慢性阻塞性肺疾病(COPD)患者对噻托溴铵能倍乐■(软雾剂)与噻托溴铵吸乐■(干粉剂)吸入装置的满意度及偏好。方法一项多中心随机、开放、交叉研究。将中度至极重度COPD患者分为能倍乐■-吸乐■组与吸乐■-能倍乐■组,用患... 详细信息

目的比较中国慢性阻塞性肺疾病(COPD)患者对噻托溴铵能倍乐■(软雾剂)与噻托溴铵吸乐■(干粉剂)吸入装置的满意度及偏好。方法一项多中心随机、开放、交叉研究。将中度至极重度COPD患者分为能倍乐■-吸乐■组与吸乐■-能倍乐■组,用患者满意度和偏好调查问卷(PASAPQ)比较两组治疗第8周时PASAPQ的性能评分、PASAPQ总评分、表达偏好的患者比例、PASAPQ的总体满意度评分及治疗后愿意继续使用的得分。结果共纳入71例COPD患者,其中能倍乐■-吸乐■组36例,吸乐■-能倍乐■组35例。基线资料显示两组性别、年龄,肺功能及慢性阻塞性肺疾病全球倡议(GOLD)分级,既往糖皮质激素/长效β2受体激动剂(ICS/LABA)的使用百分比等基线资料比较两组间无统计学差异。第8周时,PASAPQ性能评分、总评分和总体满意度评分在两组均无显著性差异。第8周时,35例(50.7%)患者偏好使用能倍乐■,26例(37.7%)患者偏好使用吸乐■,差异无统计学意义(P=0.249)。结论COPD患者对两种装置的满意度无明显差异,但患者掌握能倍乐■的操作方法后,有偏好使用能倍乐■的倾向性。

关键词：慢性阻塞性肺疾病噻托溴铵吸入装置患者满意度和偏好度

Hymecromone:a clinical prescription hyaluronan inhibitor for efficiently blocking COVID-19 progression

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Signal Transduction and Targeted Therapy 2022年第4期7卷 1287-1296页

作者： Shuai Yang Yun Ling Fang Zhao Wei Li Zhigang song Lu Wang Qiuting Li Mengxing Liu Ying Tong Lu Chen Daoping Ru Tongsheng Zhang Kaicheng Zhou Baolong Zhang Peng Xu Zhicong Yang Wenxuan Li yuanlin song Jianqing Xu Tongyu Zhu Fei Shan Wenqiang Yu Hongzhou Lu Laboratory of RNA Epigenetics Institutes of Biomedical Sciences&Shanghai Public Health Clinical Center&Department of General SurgeryHuashan HospitalCancer Metastasis Institute Shanghai Medical CollegeFudan UniversityShanghaiP.R.China Shanghai Public Health Clinical Center Fudan UniversityShanghaiP.R.China Department of Radiology Shanghai Public Health Clinical CenterFudan UniversityShanghaiP.R.China Zhongshan Hospital Fudan UniversityShanghaiP.R.China The Third People’s Hospital of Shenzhen ShenzhenP.R.China

Currently,there is no effective drugs for treating clinically COVID-19 except *** previously revealed that human identical sequences of SARS-CoV-2 promote the COVID-19 progression by upregulating hyaluronic acid(HA).As the inhibitor of HA synthesis,hymecromone is an approved prescription drug used for treating biliary ***,we aimed to investigate the relation between HA and COVID-19,and evaluate the therapeutic effects of hymecromone on ***,HA was closely relevant to clinical parameters,including lymphocytes(n=158;r=−0.50;P<0.0001),C-reactive protein(n=156;r=0.55;P<0.0001),D-dimer(n=154;r=0.38;P<0.0001),and fibrinogen(n=152;r=0.37;P<0.0001),as well as the mass(n=78;r=0.43;P<0.0001)and volume(n=78;r=0.41;P=0.0002)of ground-glass opacity,the mass(n=78;r=0.48;P<0.0001)and volume(n=78;r=0.47;P<0.0001)of consolidation in patient with low level of hyaluronan(HA<48.43 ng/mL).Furthermore,hyaluronan could directly cause mouse pulmonary ***,hymecromone remarkably reduced HA via downregulating HAS2/HAS3 ***,89%patients with hymecromone treatment had pulmonary lesion absorption while only 42%patients in control group had pulmonary lesion absorption(P<0.0001).In addition,lymphocytes recovered more quickly in hymecromone-treated patients(n=8)than control group(n=5)(P<0.05).These findings suggest that hymecromone is a promising drug for COVID-19 and deserves our further efforts to determine its effect in a larger cohort.

关键词： prescription clinical identical

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网络思想政治教育的文化价值及其实现途径

高校理论战线 2010年第11期 47-51页

作者：宋元林湖南科技大学 411203

网络思想政治教育的文化价值主要体现在对优秀民族文化的传承、对外来优秀文化的吸收、对多样文化的主导和对新文化的创造等方面,其实现途径主要包括培育网民正确的网络思想政治教育文化价值观念、建立先进文化网络传播体系和构建先进... 详细信息

网络思想政治教育的文化价值主要体现在对优秀民族文化的传承、对外来优秀文化的吸收、对多样文化的主导和对新文化的创造等方面,其实现途径主要包括培育网民正确的网络思想政治教育文化价值观念、建立先进文化网络传播体系和构建先进文化网络传播机制。

关键词：网络思想政治教育文化价值实现途径