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Expression of Mammaglobin and Carcinoembryonic Antigen in Peripheral Blood of Patients with Breast Cancer Using Real Time Polymerase Chain Reaction

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Open Journal of Clinical Diagnostics 2017年第4期7卷 103-112页

作者： Negin Khosravi Naghmeh Bahrami Adnan Khosravi Atefeh Abedini Arda Kiani somayeh sharifynia Pegah Gharaeeyan Sharareh Seifi Abdolreza Mohamadnia Islamic Azad University Tehran Iran Craniomaxillofacial Research Center Tehran University of Medical Sciences Tehran Iran Oral and Maxillofacial Surgery Department School of Dentistry Tehran University of Medical Sciences Tehran Iran Iranian Tissue Bank & Research Center Tehran University of Medical Sciences Tehran Iran Tobacco Prevention and Control Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences Tehran Iran Tracheal Diseases Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences Tehran Iran Clinical Tuberculosis and Epidemiology Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences Tehran Iran Chronic Respiratory Diseases Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences Tehran Iran Virology Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences Tehran Iran Department of Biotechnology School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences Tehran Iran

Background and Objectives: Breast cancer is among the most common causes of cancer related mortality in women worldwide. Early detection and prompt diagnosis of tumor is the first step to prevent cancer-related morbidity and mortality, and a comprehensive understanding of the involved molecular mechanisms can greatly help in this respect. Breast cancer, like many other types of cancer, is caused by a combination of genetic and epigenetic changes such as inactivation of tumor suppressor genes. Materials and Methods: This study was performed on 40 breast cancer patients and 40 healthy controls. Quantitative real time reverse transcription polymerase chain reaction (real time qRT-PCR) was used to assess the expression of carcinoembryonic antigen (CEA) and mammaglobin mRNA in the peripheral blood of patients and healthy controls. The two groups were compared using t-test. Results: The two groups were not significantly different in terms of the mean age. Twenty-nine out of 40 cancer patients were positive for CEA mRNA and its sensitivity was calculated to be 72.5%. Twelve out of 40 healthy controls were positive for CEA mRNA. Twenty-six out of 40 patients were positive for mammaglobin mRNA indicative of 65% sensitivity while only five out of 40 healthy controls were positive for mammaglobin mRNA. Conclusion: Both CEA and mammaglobin mRNA had high sensitivity in cancer patients;thus, they can be used for screening and early detection of breast cancer patients. Further studies with larger sample sizes are required to confirm the current findings.

关键词： Breast Cancer Biomarker Mammaglobin mRNA Carcinoembryonic Antigen mRNA

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Human Epididymis Protein-4 Gene Expression as a Biomarker in Patients with Ovarian Cancer in Iran

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Open Journal of Clinical Diagnostics 2017年第3期7卷 83-90页

作者： Arezo Shahi Naghmeh Bahrami Atefeh Fakharian somayeh sharifynia Elham Moslemi Amir Izadi Adnan Khosravi Hamidreza Jamaati Abdolreza Mohamadnia Department of Biology Faculty of Basic Sciences Islamic Azad University North Tehran Branch Tehran Iran Craniomaxillofacial Research center Tehran University of Medical Sciences Tehran Iran Oral and Maxillofacial Surgery Department School of Dentistry Tehran University of Medical Sciences Tehran Iran Chronic Respiratory Diseases Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences Tehran Iran Clinical Tuberculosis and Epidemiology Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences Tehran Iran Department of Biology Islamic Azad University Tehran East Tehran Iran Young Researchers and Scholars Club Islamic Azad University Tehran East Tehran Iran Bunge Oxir Research Institute Iran Tobacco Prevention and Control Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences Tehran Iran Virology Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences Tehran Iran Department of Biotechnology School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences Tehran Iran

Introduction: Ovarian cancer is one of the most common malignancies in women and the fifth cause of cancer-related deaths in women worldwide. Contrary to the challenges in developing new clinical markers using the conventional methods, recent advances in genomics and proteomics have led to identification of candidate and promising biomarkers for the diagnosis of ovarian cancer. Human epididymis protein 4 (HE4) is such a marker that has recently been reported to correlate with recurrence or progression of epithelial ovarian cancer. The purpose of this study was to measure the expression level of HE4 gene in women with ovarian cancer. Methodology: We evaluated and compared paraffin-embedded tissue samples of 20 ovarian cancer patients with 10 samples from healthy individuals. RNA was initially extracted from the samples and cDNA was synthetized. Gene expression level was then measured using real-time polymerase chain reaction (PCR). Results: Our results demonstrated that HE4 gene expression level was significantly higher in samples of patients with ovarian cancer compared with samples from healthy individuals. Moreover, higher levels of HE4 gene expression were associated with more advanced disease and larger tumor size. Conclusion: HE4 gene over-expression has the potential to be used as a biomarker for detecting early-stage ovarian cancer in women. Future more comprehensive studies are needed to confirm our findings.

关键词： Ovarian Neoplasms Genomics Tumor Biomarkers HE4 Protein

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Two Molecular Markers of Early Non-Small Cell Lung Carcinoma Based on Gene Expression in Peripheral Blood

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Open Journal of Clinical Diagnostics 2017年第3期7卷 91-102页

作者： Naghmeh Bahrami Hamidreza Jamaati Jalal Heshmat Nia Payam Tabarsi Atefeh Fakharian Habib Emami somayeh sharifynia Masoum Abniki Abdolreza Mohamadnia Craniomaxillofacial Research center Tehran University of Medical Sciences Tehran Iran Oral and Maxillofacial Surgery Department School of Dentistry Tehran University of Medical Sciences Tehran Iran Chronic Respiratory Diseases Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences Tehran Iran Clinical Tuberculosis and Epidemiology Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences Tehran Iran Tobacco Prevention and Control Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences Tehran Iran Department of Biotechnology Faculty of Sciences Islamic Azad University Tehran Iran Virology Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences Tehran Iran Department of Biotechnology School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences Tehran Iran

Background: Lung cancer is among the most common cancers. Search is ongoing to find biomarkers to improve the diagnosis lung cancer techniques in early stages. In this study we evaluate the sensitivity and specificity of the MUC1 and CEA gene expressions in the peripheral blood of non-small cell lung cancer (NSCLC). Material and Methods: This study was done in Masih Daneshvari Hospital, Tehran, Iran and was case/control study that conducted on 30 NSCLC patients and 30 healthy controls. Peripheral blood was collected and total RNA was extracted then cDNA was synthesized. Sample was separately assessed by real time PCR. Results: The expression of CEA gen was positive in 24 patients indicating 80% sensitivity for this marker. The expression of CEA gen was positive in 9 controls out of 30 each. A statistically significant difference was detected between patients and healthy controls with regard to CEA mRNA expression (P 0.001). The MUC1 gen expressed in 20 out of 30 patients, while it expressed in 3 controls. The difference in MUC1 mRNA expression was statistically significant between NSCLC patients and healthy controls (P 0.001). Conclusion: MUC1 and CEA are molecular biomarkers with relatively favorable sensitivity for primary diagnosis of NSCLC.

关键词： MUC1 mRNA CEA mRNA Non-Small Cell Lung Cancer Biomarker

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