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辣椒杂志 2016年第3期14卷 40-50页

作者： Woonhee Baek sohee lim Sung Chul Lee 刘越孔秋生中央大学生命科学系华中农业大学园艺林学学院

植物时常受到高盐和干旱等各种各样的环境胁迫,因此植物也进化出了各种防御机制以应对胁迫所带来的毒害作用。脱落酸(ABA)是一种植物激素,调控植物的生长发育并对非生物胁迫产生响应。本研究从经ABA处理的甜辣椒(Capsicum annuum)叶片... 详细信息

植物时常受到高盐和干旱等各种各样的环境胁迫,因此植物也进化出了各种防御机制以应对胁迫所带来的毒害作用。脱落酸(ABA)是一种植物激素,调控植物的生长发育并对非生物胁迫产生响应。本研究从经ABA处理的甜辣椒(Capsicum annuum)叶片中鉴定出了一个耐旱基因Ca DRT1(Drought Tolerence 1)。Ca DRT1基因在叶片中的表达受环境胁迫大幅诱导。经ABA处理后,Ca DRT1在辣椒叶片中大量表达,表明Ca DRT1蛋白在非生物胁迫响应中具有着重要功能。通过病毒诱导基因沉默(VIGS)技术沉默Ca DRT1基因后,叶片气孔关闭受限,蒸腾失水量上升,植株受到显著伤害。Ca DRT1过表达(Ca DRT1-OX)的拟南芥在发芽期、幼苗期及成株阶段均呈ABA敏感的表型。此外,Ca DRT1-OX植株呈耐旱表型,其特点是气孔关闭程度高,蒸腾率低,叶片温度高,叶片中干旱应答基因表达量增加。上述结果表明Ca DRT1在ABA介导的干旱胁迫响应中起正向调控的作用。

关键词： ABA CaDRT1 干旱胁迫辣椒病毒诱导基因沉默

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Model development to estimate site index values for six major tree species in North Korea

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Journal of Forestry Research 2023年第2期34卷 387-400页

作者： sohee Park A-Ram Yang Eun-hee Kim Joongbin lim Division of Global Forestry Department of Future Forest StrategyNational Institute of Forest ScienceSeoul 02455Republic of Korea Forest ICT Research Center National Institute of Forest ScienceSeoul 02455Republic of Korea

Due to considerable deforestation in North Korea,there is a need to plan forest restoration programs based on scientific forest *** this study,a methodology was developed for estimating the site index values of six major tree species and the forest productivity *** site index values of these tree species were derived in South Korea using the Chapman-Richards *** values were used with data from the 6th National Forest Inventory,which included 20 types of edaphic,topographic,and climatic factors,and random forest analysis—a widely used machine learning technique for spatial prediction—to develop a new model for estimating the site index values of these species across South *** prediction accuracy of this model was evaluated using the root mean square *** results show that the prediction accuracy was high,with a root mean square error of~***,the importance of the variables related to climate and geography was generally *** proposed site index estimation model for six tree species was applied across North Korea,and its effectiveness tested by comparing the estimated values with those reported in literature from North *** differences between the model outputs and recorded data in the northern alpine regions were presumably due to the lack of data for high-altitude regions in South *** model is based on the determination of the suitability of tree species in restoration ***,it can contribute to the evaluation of forest productivity in North Korea and may help plan efficient forest restoration programs.

关键词： Environmental factors Forest productivity Machine learning North Korea Random forest Site index

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The effects of single versus combined therapy using lim-kinase 2 inhibitor and type 5 phosphodiesterase inhibitor on erectile function in a rat model of cavernous nerve injury-induced erectile dysfunction

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Asian Journal of Andrology 2019年第5期21卷 493-500页

作者： Min Chui Cho Junghoon Lee Juhyun Park sohee Oh Ji Sun Chai Hwancheol Son Jae-Seung Paick Soo Woong Kim Department of Urology Seoul National University College of MedicineSMG-SNU Boramae Medical CenterSeoul 07061Korea Department of Urology Seoul National University College of MedicineSeoul National University HospitalSeoul 03080Korea Department of Biostatistics Seoul National University College of MedicineSMG-SNU Boramae Medical CenterSeoul 07061Korea

We aimed to determine whether combination of lim-kinase 2 inhibitor(limK2i)and phosphodiesterase type-5 inhibitor(PDE5i)could restore erectile function through suppressing cavernous fibrosis and improving caver nous apoptosis in a rat model of cavernous nerve crush injury(CNCI).Seventy 12-week-old Sprague-Dawley rats were equally distributed into five groups as follows:(1)sham surgery(Group S),(2)CNCI(Group I),(3)CNCI treated with daily intraperitoneal administration of 10.0 mg kg^-1 limK2i(Group I+L),(4)daily oral administration of 20.0 mg kg-1 udenafil,PDE5i(Group I+U),and(5)combined administration of 10.0 mg kg^-1 limK2i and 20.0 mg kg^-1 udenafil(Group I+L+U).Rats in Groups I+L,I+U,and I+L+U were treated with respective regimens for 2 weeks after *** 2 weeks after surgery,erectile response was assessed using *** tissues were processed for histological studies and western *** I showed lower intracavernous pressure(ICP)/mean arterial pressure(MAP),lower area under the curve(AUC)/MAP,decreased immunohistochemical staining for alpha-smooth muscle(SM)actin,higher apoptotic index,lower SM/collagen ratio,increased phospho-limK2-positive fibroblasts,decreased protein kinase B/endothelial nitric oxide synthase(Akt/eNOS)phosphorylation,increased limK2/cofilin phosphorylation,and increased protein expression of fibronectin,compared to Group *** all three treatment groups,erectile responses,protein expression of fibronectin,and SM/collagen ratio were *** I+L+U showed greater improvement in erectile response than Group I+*** content and apoptotic index in Groups I+U and I+L+U were improved compared to those in Group ***,Group I+L did not show a significant improvement in SM content or apoptotic *** number of phospho-limK2-positive fibroblasts was normalized in Groups I+L and I+L+U,but not in Group I+***/eNOS phosphorylation was improved in Groups I+U and I+L+U,but not in Group I+***2/cofilin phosphorylation was i

关键词： apoptosis erectile dysfunction fibrosis lim kinase phosphodiesterase

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