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Dedifferentiated fat cells: an alternative source of adult multipotent cells from the adipose tissues

International Journal of Oral Science 2011年第3期3卷 117-124页

作者： Jie-fei Shen Atsunori Sugawara Joe Yamashita Hideo Ogura soh sato Department of Periodontology School of Life Dentistry at Niigata Nippon Dental University Niigata 951-1500 Japan Department of Dental Materials School of Life Dentistry at Niigata Nippon Dental University Niigata 951-1500 Japan

When adipose-derived stem cells （ASCs） arc retrieved from the stromal vascular portion of adipose tissue, a large amount of mature adipocytes are often discarded. However, by modified ceiling culture technique based on their buoyancy, mature adipocytes can be easily isolated from the adipose cell suspension and dediffercn- tiated into lipid-frce fibroblast-like cells, named dediffercntiated fat （DFAT） cells. DFAT cells rc-establish active proliferation ability and undertake multipotent capacities. Compared with ASCs and other adult stem cells, DFAT cells showed unique advantages in their abundance, isolation and homogeneity. In this concise review, the establishment and culture methods of DFAT cells arc introduced and the current profiles of their cellular nature are summarized. Under proper inducti~,n culture in vitro or environment in vivo, DFAT cells could demonstrate adipogenic, osteogenic, chondrogenie and myogenic potentials. In angiogenie conditions, DFAT cells could exhibit perivascular characteristics antt elicit neovascularization. Our preliminary findings also suggested the pericyte phenotype underlying such cell lineage, which supported a novel interpretation about the common origin of mesenchymal stem cells and tissue-specific stem cells within blood vessel walls. Current research on DFAT cells indicated that this alternative source of adult multipotent cells has great potential in tissue engineering and regenerative medicine.

关键词： dedifferentiated fat cells adult stem cells adipose tissue mesenchymal stem cells regenerative medicine

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Evaluation of deoxyribonuclease activity in seminal plasmaof ejaculated chicken semen

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Asian Journal of Andrology 2003年第3期5卷 213-216页

作者： Fuminori sato Tomoki soh Masa-aki Hattori Noboru Fujihara Laboratory of Reproductive Physiology and Biotechnology Department of Animal and Marine Bioresource SciencesFaculty of Agriculture Graduate School Kyushu University Hakozaki Fukuoka 8128581 JapanLaboratory of Reproductive Physiology and Biotechnology Department of Animal and Marine Bioresource SciencesFaculty of Agriculture Graduate School Kyushu University Hakozaki Fukuoka 8128581 JapanLaboratory of Reproductive Physiology and Biotechnology Department of Animal and Marine Bioresource SciencesFaculty of Agriculture Graduate School Kyushu University Hakozaki Fukuoka 8128581 JapanLaboratory of Reproductive Physiology and Biotechnology Department of Animal and Marine Bioresource SciencesFaculty of Agriculture Graduate School Kyushu University Hakozaki Fukuoka 8128581 Japan

Aim: To confirm the stability of exogenous genes in the generation of transgenic chickens using ejaculated chicken sperm, the deoxyribonuclease (DNase) activity was evaluated in the seminal plasma of ejaculated semen and the stability of DNA was examined by adding lipofection reagents. Methods: A PCR fragment (249 bp) of pEGFPN-1 vector was used as the DNA substrate and was incubated with the seminal plasma at 40 ℃ for 30 min. Then, the whole reaction solution was subjected to agarose gel electrophoresis and the DNA size was evaluated under UV light. Results: The DNA substrate was completely diminished after incubation with seminal plasma. However, the substrate was intact after incubation with heat-treated seminal plasma or incubation with seminal plasma in the presence of 0.5 mmol/L -5 mmol/L EDTA. The substrate was stabilized in the seminal plasma by the addition of commercially available lipofection reagents. Conclusion: The DNase activity is present in the seminal plasma of ejaculated chicken semen. However, DNA is stable in the liposomal-DNA complex.

关键词： deoxyribonucleases chickens seminal plasma sperm vectors lipofection

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Identification of a novel anterograde transport signal on Emp24p.

Identification of a novel anterograde transport signal on Em...

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ThirdCongressoftheAsian...

作者： Nobuhiro Nakamua soh Yamazaki Ken sato Akihiko Nakano Masao Sakaguchi Katsuyoshi Mihara Department of Molecular Biology Graduate School of Medical ScienceKyushu UniversityFukuoka 812-82Japan Department of Molecular Biology Graduate School of Medical ScienceKyushu UniversityFukuoka 812-82Japan Molecular Membrane Biology Laboratory RIKENWakoSaitama 351-01Japan Molecular Membrane Biology Laboratory RIKENWakoSaitama 351-01Japan Department of Molecular Biology Graduate School of Medical ScienceKyushu UniversityFukuoka 812-82Japan Department of Molecular Biology Graduate School of Medical ScienceKyushu UniversityFukuoka 812-82Japan

＜正＞To examine the possibility of the active transport of Emp24p between the ER and the Golgi, we sought to identify transport signal（s） in the carboxyl-terminal region of Emp24p.

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