检索结果-南通市图书馆

Single-cell and spatial omics:exploring hypothalamic heterogeneity

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第6期20卷 1525-1540页

作者： Muhammad Junaid Eun Jeong lee Su Bin Lim Department of Biochemistry&Molecular Biology Ajou University School of MedicineSuwonSouth Korea Department of Biomedical Sciences Graduate School of Ajou UniversitySuwonSouth Korea Department of Brain Science Ajou University School of MedicineSuwonSouth Korea

Elucidating the complex dynamic cellular organization in the hypothalamus is critical for understanding its role in coordinating fundamental body functions. Over the past decade, single-cell and spatial omics technologies have significantly evolved, overcoming initial technical challenges in capturing and analyzing individual cells. These high-throughput omics technologies now offer a remarkable opportunity to comprehend the complex spatiotemporal patterns of transcriptional diversity and cell-type characteristics across the entire hypothalamus. Current single-cell and single-nucleus RNA sequencing methods comprehensively quantify gene expression by exploring distinct phenotypes across various subregions of the hypothalamus. However, single-cell/single-nucleus RNA sequencing requires isolating the cell/nuclei from the tissue, potentially resulting in the loss of spatial information concerning neuronal networks. Spatial transcriptomics methods, by bypassing the cell dissociation, can elucidate the intricate spatial organization of neural networks through their imaging and sequencing technologies. In this review, we highlight the applicative value of single-cell and spatial transcriptomics in exploring the complex molecular-genetic diversity of hypothalamic cell types, driven by recent high-throughput achievements.

关键词： cellular diversity hypothalamus multi-omics single-cell transcriptomics spatial transcriptomics

A functional tacrolimus-releasing nerve wrap for enhancing nerve regeneration following surgical nerve repair

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第1期20卷 291-304页

作者： Simeon C.Daeschler Katelyn J.W.so Konstantin Feinberg Marina Manoraj Jenny Cheung Jennifer Zhang Kaveh Mirmoeini JPaul Santerre Tessa Gordon Gregory HBorschel SickKids Research Institute Neuroscience and Mental Health ProgramTorontoONCanada Institute of Biomaterials and Biomedical Engineering University of TorontoTorontoONCanada Indiana University School of Medicine IndianapolisINUSA Division of Plastic and Reconstructive Surgery the Hospital for Sick ChildrenTorontoONCanada Translational Biology and Engineering Program Ted Rogers Centre for Heart ResearchInstitute of Biomedical EngineeringFaculty of DentistryUniversity of TorontoTorontoONCanada

Axonal regeneration following surgical nerve repair is slow and often incomplete,resulting in poor functional recovery which sometimes contributes to lifelong ***,there are no FDA-approved therapies available to promote nerve *** accelerates axonal regeneration,but systemic side effects presently outweigh its potential benefits for peripheral nerve *** authors describe herein a biodegradable polyurethane-based drug delivery system for the sustained local release of tacrolimus at the nerve repair site,with suitable properties for scalable production and clinical application,aiming to promote nerve regeneration and functional recovery with minimal systemic drug *** is encapsulated into co-axially electrospun polycarbonate-urethane nanofibers to generate an implantable nerve wrap that releases therapeutic doses of bioactive tacrolimus over 31 *** and drug loading are adjustable for applications in small and large caliber nerves,and the wrap degrades within 120 days into biocompatible *** released from the nerve wrap promotes axon elongation in vitro and accelerates nerve regeneration and functional recovery in preclinical nerve repair models while off-target systemic drug exposure is reduced by 80%compared with systemic *** its surgical suitability and preclinical efficacy and safety,this system may provide a readily translatable approach to support axonal regeneration and recovery in patients undergoing nerve surgery.

关键词： biodegradable local drug delivery nerve injury nerve regeneration nerve wrap tacrolimus

Generation of brain vascular heterogeneity:recent advances from the perspective of angiogenesis

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第7期20卷 2013-2014页

作者： Nathanael J.lee Ryota L.Matsuoka Department of Neurosciences Lerner Research InstituteCleveland ClinicClevelandOHUSA Department of Molecular Medicine Cleveland Clinic Lerner College of MedicineCase Western Reserve UniversityClevelandOHUSA

Heterogeneous proper t i es of vascular endothelial cells in the brain:The brain displays large energy dynamics and consumption,and this high level of metabolic demands is fulfilled by a continuous supply of glucose and oxygen through its vascular *** vasculature consists of highly divergent blood vessel branches,giving rise to a dense network of capillaries that supply blood to all cells across the *** elaborated vascular network is thought to develop via angiogenesis,a process in which new blood vessels grow from pre-existing *** capillaries exhibit organotypic features distinct from other tissues and are formed primarily by two major endothelial cell(EC)types:those that form the semi-permeable blood-brain barrier(BBB)and those that develop highly permeable pores known as fenestrae(Matsuoka et al.,2022).The structural and functional differences between BBB and fenestrated vascular ECs represent a fundamental feature of brain vasculature and form the foundation for both brain function and homeostasis.

关键词： angiogenesis homeostasis network

Notch ligand-expressing adenovirus infection enhances the efficacy of dendritic cell-based immunotherapy for allergic asthma in mice

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2019年第8期16卷 730-732页

作者： Chu-lun Lin Chen-Chen lee Chia-Kwung Fan Yueh-lun lee Department of Microbiology and Immunology School of MedicineCollege of MedicineTaipei Medical UniversityTaipei 11031 TaiwanChina Department of Microbiology and Immunology School of MedicineChina Medical UniversityTaichung 40402 TaiwanChina Department of Molecular Parasitology and Tropical Medicine School of MedicineCollege of MedicineTaipei Medical UniversityTaipei 11031 TaiwanChina

According to the World Health Organization,the prevalence of asthma is rising worldwide,and asthma causes 250,000 deaths annually.1 Allergic asthma is a type of chronic airway inflammation caused by dysregulated T helper type 2(Th2)-type immune *** with allergic asthma are characterized by elevated serum immunoglobulin E(IgE)antibody levels,mucus production,and pulmonary inflammation as well as airway hyperresponsiveness(AHR).2 Current therapeutic methods for asthma include inhaled corticosteroids andβ2 agonists to relieve asthma syndromes,but these treatments do not change the chronic disease course in *** patients need high doses of inhaled corticosteroids or require long-term daily treatment with oral steroids.3 Therefore,efforts should be targeted towards developing innovative therapeutic strategies to improve patient quality of life and reduce unwanted *** of the advances in the development of future therapies is the use of genetically modified dendritic cells(DCs)to decrease pathological Th2-mediated responses and maintain a disease-modifying effect long *** activation is crucial for inducing T cell immunity as well as determining T cell differentiation.4 Importantly,DCs can be cultured in vitro,and large amounts of generated clinical-grade DCs have become ***,DCs has been shown to be permissive of adenoviral(Ad)vector infection in vitro,and Ad vectors are a promising gene delivery platform for a variety of therapeutic and vaccine applications.5 In this regard,overexpressing immunoregulatory proteins in Ad-modified DCs would be an effective way to suppress Th2 cell immunity and establish an antiallergic response.

关键词： asthma allergic immunity

Tailoring Light–Matter Interactions in Overcoupled Resonator for Biomolecule Recognition and Detection

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano-Micro Letters 2025年第1期17卷 262-280页

作者： Dongxiao Li Hong Zhou Zhihao Ren Cheng Xu Chengkuo lee Department of Electrical and Computer Engineering National University of SingaporeSingapore 117583Singapore Center for Intelligent Sensors and MEMS(CISM) National University of SingaporeSingapore 117608Singapore

Plasmonic nanoantennas provide unique opportunities for precise control of light–matter coupling in surface-enhanced infrared absorption(SEIRA)spectroscopy,but most of the resonant systems realized so far suffer from the obstacles of low sensitivity,narrow bandwidth,and asymmetric Fano resonance ***,we demonstrated an overcoupled resonator with a high plasmon-molecule coupling coefficient(μ)(OC-Hμresonator)by precisely controlling the radiation loss channel,the resonator-oscillator coupling channel,and the frequency detuning *** observed a strong dependence of the sensing performance on the coupling state,and demonstrated that OC-Hμresonator has excellent sensing properties of ultra-sensitive(7.25%nm^(−1)),ultra-broadband(3–10μm),and immune asymmetric Fano *** characteristics represent a breakthrough in SEIRA technology and lay the foundation for specific recognition of biomolecules,trace detection,and protein secondary structure analysis using a single array(array size is 100×100μm^(2)).In addition,with the assistance of machine learning,mixture classification,concentration prediction and spectral reconstruction were achieved with the highest accuracy of 100%.Finally,we demonstrated the potential of OC-Hμresonator for SARS-CoV-2 *** findings will promote the wider application of SEIRA technology,while providing new ideas for other enhanced spectroscopy technologies,quantum photonics and studying light–matter interactions.

关键词： Plasmonic nanoantennas Light-matter interaction Surface-enhanced infrared absorption Overcoupled Biosensing

EFFECT OF YELLOW LOESS ON ODOROUS GAS ADsoRPTION AND ECOTOXICITY TEST USING DAPHNIA MAGNA

在线全文

学校读者我要写书评

暂无评论

Journal of Green Building 2021年第1期16卷 103-114页

作者： Hongseok Jang Boram lee Seungyoung so Jeonbuk National University Department of Architectural EngineeringKorea. Jeonbuk National University Department of Architectural EngineeringResearch Center of Industrial TechnologyKorea

To minimize damage caused by harmful substances released from artificial construc-tion materials,there has been increasing interest in eco-friendly houses constructed with natural *** natural materials,yellow loess is south Korea’s most representative eco-friendly construction material,with high purification performance for indoor ***,there has been no objective assessment of yellow loess’s performance at purifying indoor *** this study,internal pores were found in yellow loess that were of a lamellar crystal structure consisting of bonds of silica and alumina plates,and there was a high negative charge on the loess surface;these might have contributed to the excellent performance of yellow loess in adsorbing odorous *** was also an exposure test using Daphnia magna to determine the eco-friendliness of yellow loess compared with that of cement *** showed the survival of Daphnia magna was longer in the indoor environment of a test chamber with higher concentration of ammonia wherein yellow loess was ***50(median effective concentration,the concentration that caused death of 50%of bio-specimens)value of 19 ml of ammonia(0.1%solution),which was three times more than EC50 value of 6 ml of ammonia of the same area of cement mortar.

关键词： eco-friendly material yellow loess Daphnia magna evaluation CEC gas adsorption

Neuroprotection by resveratrol-glucuronide and quercetin-glucuronide via binding to polyphenol-and glycosaminoglycan-binding sites in the laminin receptor

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第3期20卷 819-820页

作者： Rayudu Gopalakrishna Jennifer Aguilar Emily lee William J.Mack Department of Integrative Anatomical Sciences Keck School of MedicineUniversity of Southern CaliforniaLos AngelesCAUSA Department of Neurological Surgery Keck School of MedicineUniversity of Southern CaliforniaLos AngelesCAUSA

The dietary polyphenolic compounds resveratrol and quercetin prevent neurodegenerative diseases in experimental models;however, they reach the brain only in nanomolar concentrations in the glucuronidated and sulfated ... 详细信息

关键词： resveratrol molar dated

Peak earthquake response of structures under multi-component excitations

在线全文

学校读者我要写书评

暂无评论

Earthquake Engineering and Engineering Vibration 2007年第4期6卷 357-370页

作者： Jianwei song Zach Liang Yi-lun Chu George C. lee Department of Civil Structural and Environmental Engineering State University of New York at Buffalo USA

Accurate estimation of the peak seismic responses of structures is important in earthquake resistant design. The internal force distributions and the seismic responses of structures are quite complex, since ground motions are multidirectional. One key issue is the uncertainty of the incident angle between the directions of ground motion and the reference axes of the structure. Different assumed seismic incidences can result in different peak values within the scope of design spectrum analysis for a given structure and earthquake ground motion record combination. Using time history analysis to determine the maximum structural responses excited by a given earthquake record requires repetitive calculations to determine the critical incident angle. This paper presents a transformation approach for relatively accurate and rapid determination of the maximum peak responses of a linear structure subjected to three-dimensional excitations within all possible seismic incident angles. The responses can be deformations, internal forces, strains and so on. An irregular building structure model is established using SAP2000 program. Several typical earthquake records and an artificial white noise are applied to the structure model to illustrate the variation of the maximum structural responses for different incident angles. Numerical results show that for many structural parameters, the variation can be greater than 100%. This method can be directly applied to time history analysis of structures using existing computer software to determine the peak responses without carrying out the analyses for all possible incident angles. It can also be used to verify and/or modify aseismic designs by using response spectrum analysis.

关键词： multi-component ground motion time history analysis peak structural response axis rotation

Context Awareness by Noise-Pattern Analysis of a Smart Factory

在线全文

学校读者我要写书评

暂无评论

Computers, Materials & Continua 2023年第8期76卷 1497-1514页

作者： so-Yeon lee Jihoon Park Dae-Young Kim Department of software Convergence Soonchunhyang UniversityAsan31538Korea Department of Computer software Engineering Soonchunhyang UniversityAsan31538Korea

Recently,to build a smart factory,research has been conducted to perform fault diagnosis and defect detection based on vibration and noise signals generated when a mechanical system is driven using deep-learning technology,a field of artificial *** of the related studies apply various audio-feature extraction techniques to one-dimensional raw data to extract sound-specific features and then classify the sound by using the derived spectral image as a training ***,compared to numerical raw data,learning based on image data has the disadvantage that creating a training dataset is very ***,we devised a two-step data preprocessing method that efficiently detects machine anomalies in numerical raw *** the first preprocessing process,sound signal information is analyzed to extract features,and in the second preprocessing process,data filtering is performed by applying the proposed *** efficient dataset was built formodel learning through a total of two steps of data *** addition,both showed excellent performance in the training accuracy of the model that entered each dataset,but it can be seen that the time required to build the dataset was 203 s compared to 39 s,which is about 5.2 times than when building the image dataset.

关键词： Noise-pattern recognition context awareness deep learning fault detection smart factory