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Improving gene transfection efficiency of highly branched poly(β-amino ester)s through the in-situ conversion of inactive terminal groups

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Chinese Chemical Letters 2024年第8期35卷 304-308页

作者： Zhili Li Qijun wo Dongdong Huang Dezhong Zhou Lei Guo Yeqing Mao Department of Urology The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310000China School of Chemical Engineering and Technology Xi’an Jiaotong UniversityXi’an 710049China Urology&Nephrology Center Department of UrologyZhejiang Provincial People’s Hospital(Affiliated People’s Hospital)Hangzhou Medical CollegeHangzhou 310014China Key Laboratory of Endocrine Gland Diseases of Zhejiang Province Hangzhou 310014China Pooling Institute of Translational Medicine Hangzhou 311100China

Highly branched poly(β-amino ester)s(HPAEs)have emerged as a safe and efficient type of non-viral gene delivery ***,the presence of inactive terminal secondary amine groups compromises their gene transfection *** this study,HPAEs with similar topological structures and chemical compositions but varying numbers of terminal secondary 4-amino-1-butanol(S4)and secondary/tertiary 3-morpholinopropylamine(MPA)groups were *** results demonstrate that an increased number of secondary/tertiary MPA groups in-situ significantly enhances the DNA binding capability of HPAEs,leading to the formation of smaller HPAE/DNA polyplexes with higher zeta potential,ultimately resulting in superior gene transfection efficiency in bladder epithelial *** study establishes a sim-ple yet effective strategy to maximize the gene transfection potency of HPAEs by converting the inactive terminal groups in-situ without the need for complex modifications to their topological structure and chemical composition.

关键词： Gene therapy Gene delivery vector Highly branched poly(β-amino ester)s Terminal groups Epithelial cells

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精神分裂症患者的自体诱发水中毒

国外医学.精神病学分册 1981年第4期 218-220页

作者： smith wo Clark ML 周天骍周志良

自1962年作者报导两例精神分裂症患者在没有其他疾病的情况下,自体产生饮水中毒的病例以来,陆续有17篇文献报导,共包括25例病人。这27例中16例患者至少是有暂

关键词：水中毒病人水-电解质代谢紊乱精神分裂症患者分泌异常自体

CRISPR-Cas9 gene editing strengthens cuproptosis/chemodynamic/ferroptosis synergistic cancer therapy

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Acta Pharmaceutica Sinica B 2024年第9期14卷 4059-4072页

作者： Xiaoyu Wu Zijun Bai Hui Wang Hanqing Wang Dahai Hou Yunzhu Xu Guanqun wo Haibo Cheng Dongdong Sun Weiwei Tao Department of Surgical Oncology Affiliated Hospital of Nanjing University of Chinese MedicineNanjing 210029China School of Integrated Medicine Nanjing University of Chinese MedicineNanjing 210023China Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine in Prevention and Treatment of Tumor Nanjing University of Chinese MedicineNanjing 210023China School of Pharmacology Nanjing University of Chinese MedicineNanjing 210023China School of Pharmacology Ningxia Medical UniversityYinchuan 750004China

Copper-based nanomaterials demonstrate promising potential in cancer *** efficiently triggers a Fenton-like reaction and further consumes the high level of glutathione,initiating chemical dynamic therapy(CDT)and ***,a newly identified cell death modality that represents a great prospect in cancer therapy,is ***,active homeostatic systems rigorously keep copper levels within cells exceptionally low,which hinders the application of cooper nanomaterials-based ***,a novel strategy of CRISPR-Cas9 RNP nanocarrier to deliver cuprous ions and suppress the expression of copper transporter protein ATP7A for maintaining a high level of copper in cytoplasmic fluid is *** Cu2O and organosilica shell would degrade under the high level of glutathione and weak acidic environment,further releasing RNP and *** liberated Cut triggered a Fenton-like reaction for CDT and partially transformed to Cu2t,consuming intracellular GSH and initiating cuproptosis and ferroptosis ***,the release of RNP effectively reduced the expression of copper transporter ATP7A,subsequently increasing the accumulation of cooper and enhancing the efficacy of CDT,cuproptosis,and *** tumor microenvironment responsive multimodal nanoplatform opens an ingenious avenue for colorectal cancer therapy based on gene editing enhanced synergistic cuproptosis/CDT/ferroptosis.

关键词： GSH-and pH-responsive Effective copper delivery CRISPR-Cas9 ATP7A Cuproptosis Chemodynamic Ferroptosis Synergistic therapy

In vivo real-time monitoring delayed administration of M2 macrophages to enhance healing of tendon by NIR-II fluorescence imaging

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Nano Research 2024年第5期17卷 4379-4390页

作者： Yuzhou Chen Mo Chen Chengxuan Yu Huizhu Li Liman Sai Nguyen T.K.Thanh Yueming Wang Yan wo Jian Zhang Xing Yang Evgenii L.Guryev Andrei V.Zvyagin Hao De Min Tang Shiyi Chen Yunxia Li Yuefeng Hao Sijia Feng Jun Chen Sports Medicine Institute of Fudan University Department of Sports MedicineHuashan HospitalFudan UniversityShanghai 200040China Department of Orthopedic Surgery Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Bone and Joint Surgery Department of OrthopedicsRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200001China Department of Physics Shanghai Normal UniversityShanghai 200234China Biophysics Group Department of Physics and AstronomyUniversity College LondonLondon WC1E 6BTUK UCL Healthcare Biomagnetic and Nanomaterials Laboratories London W1S 4BSUK Department of Anatomy and Physiology School of MedicineShanghai Jiao Tong UniversityShanghai 200025China Department of Orthopedics Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou 215500China Institute of Biology and Biomedicine Lobachevsky State University of Nizhny NovgorodNizhny Novgorod 603950Russia Mathematical Institute University of OxfordOxford OX26GGUK Institute of Natural Sciences School of MathematicsShanghai Jiao Tong UniversityShanghai 200240China

The administration time is a critical but long-neglected point in cell therapy based on macrophages because the incorrect time of macrophage administration could result in diverse outcomes regarding the same macrophage *** this work,the second near-infrared(NIR-II)fluorescence imaging in vivo tracking of M2 macrophages during a pro-healing therapy in the mice model of rotator cuff injury revealed that the behavior of administrated macrophages was influenced by the timing of their *** delayed cell therapy(DCT)group had a longer retention time of injected M2 macrophages in the repairing tissue than that in the immediate cell therapy(ICT)*** Keller-Segel model and histological analysis further demonstrated that DCT altered the chemotaxis of M2 macrophages and improved the healing outcome of the repaired structure in comparison with *** results offer a possible explanation of previous conflicting results on reparative cell therapy and provoke reconsideration of the timing of these therapies.

关键词： macrophage cell therapy NIR-II in vivo imaging delayed therapy tendon healing

民族地区教师数字素养的发展现状与提升路径——基于广西9市教师样本的实证分析

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民族教育研究 2024年第1期35卷 117-124页

作者：袁磊刘沃奇广西师范大学教育学部广西桂林541004 广西师范大学教育区块链与智能技术教育部重点实验室广西桂林541004

研究采用问卷调查与深度访谈的方法,系统评估广西壮族自治区9个市3588名基础教育阶段教师的数字素养,包括数字意识、数字技术知识与技能、数字教学应用能力、数字安全与社会责任以及数字专业发展等5个核心维度。研究结果显示,广西基础... 详细信息

研究采用问卷调查与深度访谈的方法,系统评估广西壮族自治区9个市3588名基础教育阶段教师的数字素养,包括数字意识、数字技术知识与技能、数字教学应用能力、数字安全与社会责任以及数字专业发展等5个核心维度。研究结果显示,广西基础教育教师的数字素养整体上呈现积极态势,但其发展呈现不平衡性。进一步分析发现,教师的数字素养随着教龄的增长而逐渐降低,且尽管数字时代城乡教育资源差距有所缩小,但这种不均衡依旧对教师数字素养产生显著影响。针对研究发现提出以下建议:完善教师培训体系,以适应数字化时代的需求;创新教研体系,增强教师的数字教学能力;畅通数字资源平台通道,确保教育资源的公平可及;构建文化共生的教育环境,以推动教师数字素养的均衡与全面发展。

关键词：民族地区教师教育教师数字素养教师专业发展城乡数字资源不均衡

《民法典》背景下个人信息保护的司法考察与制度完善

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征信 2024年第5期42卷 31-42页

作者：沃耘乔鹏飞天津商业大学法学院天津市300134

大数据时代,为充分保障个人的信息安全,我国相继施行的《民法典》《个人信息保护法》对此作出了较为全面的规定。考察相关规则的司法适用,发现我国个人信息保护存在个人信息与隐私权界定不清、损害赔偿威慑作用不足、公益诉讼制度效用... 详细信息

大数据时代,为充分保障个人的信息安全,我国相继施行的《民法典》《个人信息保护法》对此作出了较为全面的规定。考察相关规则的司法适用,发现我国个人信息保护存在个人信息与隐私权界定不清、损害赔偿威慑作用不足、公益诉讼制度效用未充分发挥、“知情—同意”标准不一且流于形式等问题。为解决上述问题,可通过构建可识别的场景化模式界定个人信息,引入情境脉络完整性理论判定隐私,审慎引入惩罚性赔偿,明晰个人信息保护公益诉讼的适用条件,构建“国家—行业—平台”三层规则体系实现实质同意,全面保护个人信息。

关键词：个人信息保护隐私权惩罚性赔偿 “知情—同意” 《民法典》

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Evaluation of Agricultural Contamination Level by Heavy Metals and Pesticides in Sediments of Rivers and Water Bodies for Shrimp Fishing in the Lower Ouémé: Case of the Aguigadji, Ahlan and Sele Stations in Benin

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Advances in Materials Physics and Chemistry 2024年第2期14卷 25-46页

作者： Souradjou Orou Goura Fadéby Modeste Gouissi Armelle Sabine Yélignan Hounkpatin Wakili Bolatito Yessoufou Tayéwo Sylvain Biaou Nonvignon Martial Fassinou Laboratory of Ecology Health and Animal Production (LESPA) Faculty of Agronomy (FA) University of Parakou (UP) Parakou Bénin Pluridisciplanary Research Laboratory for Technical Education (LARPET) University of Sciences Technologies Engineering and Mathematics of Abomey (UNSTIM) Lokossa Bénin

This study assessed sediment contamination by heavy metals and pesticide active ingredients linked to chemical inputs used in agricultural activities in the lower Ouémé. Pesticide residues from the organochlorine, pyrethroid and organophosphorus families were investigated by gas chromatography, and heavy metals (Cd, Pb, As, Ni, Zn, Fe, Mg, Cr and Hg) by atomic absorption spectrophotometry. The metallic pollution indices, the contamination factor (CF) and the ecological risk index were calculated. The results revealed 8 active ingredients in the rainy season and 9 in the dry season. Glyphosate was the active ingredient with the highest concentration at all stations, 9.65 ± 0.84 mg/kg recorded in the dry season at the Aguigadji station. All glyphosate values were above the EQS. DDT, Atrazine and Endosulfan also showed high concentrations in the dry and rainy seasons. Emamectin, Abamectin and Lambda Cyhalothrin also showed high concentrations in the dry season at Aguigadji, Ahlan and Sele. Only glyphosate was recorded at the control station (Toho), but in very low concentrations. Lead showed the highest concentrations at all the stations, 265.96 ± 21.02 mg/Kg in the rainy season and 255.38 ± 79.09 mg/Kg in the dry season, all detected at the Aguigadji station and above the EQS. Zn, Ni, Fe, Cu and Cr were all representative in both the dry and rainy seasons at the contaminated stations. Manganese showed high concentrations in the rainy season. Pb showed very high contamination (FC ≥ 6) at the Aguigadji and Ahlan stations and significant contamination (3 ≤ FC 6) at the Sele station in both the rainy and dry seasons. Ni, Hg and Cd, showed either moderate or significant contamination at the contaminated stations. The risk values showed a considerable ecological Ri (190 ≤ Ri < 380) in the rainy season and a moderate ecological Ri (95 ≤ Ri < 190) in the dry season at these contaminated stations.

关键词： Pesticides Heavy Metals Sediments Shrimp Fishing Area Lower Ouémé

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“网络安全”概念的嬗变及其检视与重构

江苏警官学院学报 2024年第3期39卷 98-106页

作者：孟卧杰江苏警官学院现代警务研究中心

“网络安全”的核心内涵经历了网络系统安全时代、网络空间安全时代和网络信息安全时代的嬗变,实现了科学技术、互联网和网络信息三次视阈转换,相关研究也从信息科学技术领域延展到哲学及人文社科等领域。由此,在使用“网络安全”概念时... 详细信息

“网络安全”的核心内涵经历了网络系统安全时代、网络空间安全时代和网络信息安全时代的嬗变,实现了科学技术、互联网和网络信息三次视阈转换,相关研究也从信息科学技术领域延展到哲学及人文社科等领域。由此,在使用“网络安全”概念时,应重视稳定性与灵活性的统一,明确不同学科研究边界,根据不同范畴设定相应的法律原则、管理对象及调控方式。为实现“网络安全”目标,应按照总体国家安全观与法治要求,构建涵盖多主体行为的法律规范体系和网络安全保护制度:对于系统安全,强化技术防范与刑罚震慑;对于空间安全,设计科学有效的制度,重视公民基本权利保障,提高行政执法效率;对于信息安全,科学处理网络信息安全时代的民事、刑事和行政法律关系。

关键词：网络安全嬗变检视重构

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骨科慢病中药药学服务管理体系的构建与实施

中医药管理杂志 2024年第14期32卷 84-86页

作者：沃时佳杭州市富阳中医骨伤医院浙江杭州311400

目的:构建和实施骨科慢病中药药学服务管理体系,以推动中药在骨科的应用与推广。方法:医院骨科于2023年1月构建和实施骨科慢病中药药学服务管理体系,于实施前(2022年)和实施后(2023年)按照随机性原则分别筛选150例患者为研究对象,比较... 详细信息

目的:构建和实施骨科慢病中药药学服务管理体系,以推动中药在骨科的应用与推广。方法:医院骨科于2023年1月构建和实施骨科慢病中药药学服务管理体系,于实施前(2022年)和实施后(2023年)按照随机性原则分别筛选150例患者为研究对象,比较实施前后中药不合理用药情况、中药不良反应发生情况及中药知信行水平。结果:实施后骨科慢病中药不合理用药率低于实施前(P<0.05);实施后骨科慢病中药不良反应总发生率低于实施前(P<0.05);实施后骨科慢病患者对中药知识的掌握程度、对中药疗效和安全性的信任程度及应用中药时的行为表现评分均高于实施前(P<0.05)。结论:建立科学、有效的管理体系可以提高中药药学服务质量,对骨科慢病患者的治疗效果和生活质量具有积极影响,对促进中医药事业的发展具有重要意义。

关键词：骨科慢病中药药学服务管理体系管理流程工作规范