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Flavone improves liver damage in nicotine-exposed rats via the Nrf2/HO-1 pathway

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Asian Pacific Journal of Tropical Biomedicine 2024年第8期14卷 341-349页

作者： Nora A.Elsayed Fatma sm Moawed Esraa SA Ahmed Ahmed Hammad Omayma AR Abo-Zaid Biochemistry and Molecular Biology Department Faculty of Vet.Med.Benha UniversityBenha cityEgypt Health Radiation Research National Center for Radiation Research and TechnologyEgyptian Atomic Energy AuthorityCairoEgypt Radiation Biology Department National Center for Radiation Research and TechnologyEgyptian Atomic Energy AuthorityCairoEgypt

Objective:To assess the hepatoprotective effects of flavone on nicotine-induced liver ***:Thirty-six rats were allocated into six groups:the control group,the nicotine group,the flavone alone groups(10 and 25 mg/kg/body weight),and the nicotine groups treated with flavone(10 and 25 mg/kg/body weight).Liver function,oxidative stress,Nrf2 pathway(HO-1,Nrf2,and Keap-1),and inflammatory markers(IL-17,TNF-α,and NF-κB)were ***,a histopathological examination of liver tissues was ***:Nicotine increased liver damage,inflammation,and oxidative ***,flavone suppressed nicotine-induced liver enzymes,oxidative stress,and inflammation,as manifested by increased antioxidants and decreased malondialdehyde level,liver enzymatic activities,and inflammatory ***(10 and 25 mg/kg/body weight)also reduced the level of Keap-1 and increased HO-1 and Nrf2 levels in the liver of nicotine-exposed ***:Flavone has hepatoprotective properties and may slow the progression of liver injury by reducing oxidative stress,liver enzymes,and inflammation possibly via the Nrf2 pathway.

关键词： Flavone Nicotine Liver Nrf2 HO-1 NF-κB

PLK1 phosphorylation of ZW10 guides accurate chromosome segregation in mitosis

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Journal of Molecular Cell Biology 2024年第2期16卷 47-62页

作者： sm Faysal Bellah Fangyuan Xiong Zhen Dou Fengrui Yang Xing Liu Xuebiao Yao Xinjiao Gao Liangyu Zhang MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics Hefei National Research Center for Physical Sciences at the MicroscaleUniversity of Science and Technology of China School of Life SciencesHefei 230027China Anhui Key Laboratory for Cellular Dynamics and Chemical Biology Hefei 230027China

Stable transmission of genetic information during cell division requires faithful chromosome *** evidence has demonstrated that polo-like kinase 1(PLK1)dynamics at kinetochores control correct kinetochore–microtubule attachments and subsequent silencing of the spindle assembly ***,the mechanisms underlying PLK1-mediated silencing of the spindle checkpoint remain ***,we identified a regulatory mechanism by which PLK1-elicited zeste white 10(ZW10)phosphorylation regulates spindle checkpoint silencing in ***10 is a cognate substrate of PLK1,and the phosphorylation of ZW10 at Ser12 enables dynamic ZW10–Zwint1 *** of ZW10 phosphorylation resulted in misaligned chromosomes,while persistent expression of phospho-mimicking ZW10 mutant caused premature anaphase,in which sister chromatids entangled as cells entered *** findings reveal the previously uncharacterized PLK1–ZW10 interaction through which dynamic phosphorylation of ZW10 fine-tunes accurate chromosome segregation in mitosis.

关键词： ZW10 PLK1 kinetochore mitosis phosphorylation

Efficacy of Gold Nanoparticles against Nephrotoxicity Induced by Schistosoma mansoni Infection in Mice

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Biomedical and Environmental Sciences 2016年第11期29卷 773-781页

作者： Mohamed A Dkhil Mona F Khalil2 Amira A Bauom Marwa sm Dia Saleh A1-Qura Department of Zoology College of ScienceKing Saud UniversityRiyadh 11451Saudi Arabia Department of Zoology and Entomology Faculty of ScienceHelwan UniversityCairo 11795Egypt Department of Laboratory Sciences College of Science&ArtsA1-RassQassim UniversityAL-Qaseam 51921Saudi Arabia Molecular Drug Evaluation Department National Organization for Drug Control&Research(NODCAR)Giza 12553Egypt

Objective In this study, the ameliorative effects of gold nanoparticles （gold NP） on the renal tissue damage in Schistosoma mansoni （S. mansoni）-infected mice was investigated. Methods High-resolution transmission electron microscopy was used for the characterization of NP. The gold NP at concentrations of 250, 500, and 1000 μg/kg body weight were inoculated into 5. mansoni-infected mice. Results The parasite caused alterations in the histological architecture. Furthermore, it induced a significant reduction in the renal glutathione levels; however, the levels of nitric oxide and malondialdehyde were significantly elevated. The parasite also managed to downregulate KIM-I, NGAL, MCP-1, and TGF-8 mRNA expression in infected animals. Notably, gold NP treatment in mice reduced the extent of histological impairment and renal oxidative damage. Gold NP were able to regulate gene expression impaired by 5. Mansoni infection. Conclusion The curative effect of gold NP against renal toxicity in 5. mansoni-infected mice is associated with their role as free radical scavengers.

关键词： Gold nanoparticles Schistosomiasis Renal damage Gene expression Histopathology Oxidative stress

水痘-带状疱疹病毒编码circRNA的鉴定与表征

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中国疼痛医学杂志 2024年第8期30卷 561-562页

作者：杨少敏(译) 肖礼祖(校) Cao D Jaijyan DK 深圳大学医学部生物医学工程学院医学超声关键技术国家地方联合工程实验室广东省生物医学信息检测与超声成像重点实验室深圳大学第六附属医院(南山医院)疼痛医学重点实验室不详

在真核生物中,环状RNA(circular RNA,circRNA)是一种重要的转录本。该研究证明了水痘-带状疱疹病毒(varicella-zoster virus,VZV)感染过程中可产生病毒来源的circRNA。该研究通过联合二代与三代深度circRNA测序,绘制了高分辨率的细胞和... 详细信息

在真核生物中,环状RNA(circular RNA,circRNA)是一种重要的转录本。该研究证明了水痘-带状疱疹病毒(varicella-zoster virus,VZV)感染过程中可产生病毒来源的circRNA。该研究通过联合二代与三代深度circRNA测序,绘制了高分辨率的细胞和病毒circRNA图谱。接着,在VZV感染的细胞以及带状疱疹病人组织样品中,通过多种实验技术手段进一步验证VZV感染可产生大量病毒circRNA。此外,该研究发现了许多与VZV潜伏相关转录本同源的circRNA(circVLTSlytic)。为了分析这些circVLTSlytic的生物学功能,利用细菌人工染色体系统在基因组DNA水平,对circVLT进行突变。结果发现,circVLT是VZV复制的非必需分子,但circVLTsSlytic外显子5下游的突变可导致VZV对阿昔洛韦敏感性增加。该研究首次证明了VZV感染可产生具有生物学功能的病毒来源circRNA,这为带状疱疹的防治提供了新的思路。

关键词：病毒来源带状疱疹病细菌人工染色体阿昔洛韦感染过程 zoster VZV 转录本

Serum deprivation enhances DNA synthesis of human hepatoma smMC-7721 cells

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World Journal of Gastroenterology 1998年第2期4卷 29-32页

作者： Jiang, sm Xu, ZH Shandong Normal Univ Dept Biol Provincial Key Lab Anim Stress Jinan 250014 Shandong Provin Peoples R China

AIM To determine the relationship between serum deprivation or serum levels and cell proliferation of human hepatoma smMC 7721 cells. METHODS Human hepatoma smMC 7721 cells were grown in RPMI 1640 supplemented with 10% fetal calf (FCS) in 5% CO 2 incubator at 37℃ for 24h , and culture media were replaced to serum free or different serum FCS levels (2 5%, 5%, 10%, 20% and 25%). Six h, 12h , 18h and 24h after the culture, the cells were incorporated TdR for 4h . At last TdR incorporation was detected with liquid scintillation counting. RESULTS DNA synthesis of smMC 7721 cells could be sharply stimulated by short time (6h) serum deprivation (the cpm value of 3H TdR incorporation of cells in serum free was 39 32 fold higher than cells in 25% serum), and the incorporation of 3H TdR was negatively related to the serum levels. Longer time serum starvation ( 12h , 18h and 24h ) also greatly stimulated DNA synthesis, although the cpm value of 3H TdR incroporation was less than that in 6h serum deprivation. Morphology of cells cultured in different serum levels also showed significant difference. CONCLUSIONS Compared with other cell lines such as BEL7404 and Swiss 3T3, human hepatoma smMC 7721 cells had different response to the serum deprivation. Short time serum deprivation could greatly stimulate DNA synthesis of human hepatoma smMC 7721 cells. Precautions must be given to the changes of serum levels for the detection of growth factors and drugs using smMC 7721 cells as a model.

关键词： liver neoplasms carcinoma, hepatocellular DNA, neoplasm/biosynthesis smMC 7721 tumor cell, cultured cell proliferation growth factors

Experimental analysis of ballistic trauma in a human body protected with 30 layer packages made of biaxial and triaxial Kevlar^(■)29 fabrics

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Defence Technology（防务技术） 2023年第3期21卷 73-87页

作者： Justyna Pinkos Zbigniew Stempien Anna smędra Institute of Textile Architecture Lodz University of TechnologyLodzPoland Chair and Department of Forensic Medicine Medical University of LodzLodzPoland

Behind armour blunt trauma(BABT)is a body injury resulting from the deformation of the back surface of armour as a result of a bullet *** the case of textile body armour,the severity of the injury may depend on the material of the fibres,but also on the geometric structure of the *** article focuses on experimental research into injuries of the human body protected by ballistic packets made of biaxial and triaxial fabrics,during a non-penetrating impact from a Parabellum 9 mm×19 mm Full Metal Jacket(FMJ)bullet,at a speed of 406±5 m/*** experimental research,the fabrics had a comparable surface weight and were made of the same Kevlar^(■)29 *** ballistic packages were made of 30 *** part of the work,a physical model of the human body was *** human body model consisted of a model of the heart,lungs,and skeletal and muscular *** the bullet impact,the pressure forces were recorded using sensors located at selected points of the human *** bullets hit five selected places on the body that were considered critical,from the point of view of maintaining a human’s vital *** was found that,during firing,pressure increases both at the site of impact and in the internal organs,which can lead to multi-organ *** a result of the experimental analysis,it has been shown that the pressures exerted on specific organs are always lower in the case of body protection with a ballistic packet made of triaxial fabrics,compared to a packet made of biaxial fabrics.

关键词： protected impact organs

Binderless Carbon/Graphite Materials

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Journal of Materials Science & Technology 1997年第3期13卷 184-188页

作者： Zhou, sm Xia, JT Yan, LC Dept. of Chemistry and Chemical Engineering Hunan University Changsha 410082 China

High performance binderless carbon bodies can be manufactured from the mesophase raw material derived from petroleum pitch, which is ground into fine powders less than 10 mu m, and directly moulded, then sintered at 1000 degrees C without any kind of binder. The sintered bodies have a bulk density of 1.69 g/cm(3), bending strength of 91.2 MPa. Shore hardness of 100, porosity of only 7.2%, and electrical resistivity of 60x10(-6) Omega m. The bodies graphitized at 2800 degrees C have a bulk density of 1.88 g/cm(3), bending strength of 82 MPa. Shore hardness of 85, porosity of 5.4%, and electrical resistivity of 12x10(-6) Omega m. The scanning electron micrographs of the section show that the bodies possess an extremely dense and homogeneous structure. Infra-red spectrum analysis shows that carbonyls introduced when ground or activated are one of the key factors determining the selfbinding and selfsintering properties of the mesophase fine powders, and the structure and strength of the sintered bodies.

关键词： Carbon

Microstructures and Tensile Properties of Isothermally-forgedTi-47Al-2Nb-1Cr-1V and Ti-47Al-2V-1Cr Alloys

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Journal of Materials Science & Technology 1997年第6期13卷 452-456页

作者： Qin, GW Hao, sm Han, CX Northeastern Univ Dept Mat Sci & Engn Shenyang 110006 Peoples R China NW Inst Nonferrous Met Res Xian 710016 Peoples R China

Microstructures and room-temperature tensile properties of isothermally-forged gamma-base (gamma + alpha(2)) alloys in Ti-AI-Nb-Cr-V system with different heat treatments were investigated. The results show that the microstructures of Ti-47Al-2Nb-1Cr-1V and Ti-47Al-2V-1Cr (at. pet) alloys are mainly determined by heat treating temperature in the (alpha + gamma) two-phase field, and the joint additions of Nb, Ci and V in the Ti-47Al alloy affect T-alpha significantly. The microstructure of Ti-47Al alloy with additions of Nb, Cr and V (1 similar to 2 at. pat) can be duplex or nearly-lamellar by a suitable heat treatment after isothermal forging at 1000 degrees C for over 50% plastic strains. Therefore its tensile properties can be improved at room temperature.

关键词： Titanium alloys

Numerical Simulation of Thermal Stress of Shaped Casting Based on Rheological Model

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Journal of Materials Science & Technology 1999年第3期15卷 267-270页

作者： Kang, JW Liu, BC Xiong, sm Tsing Hua Univ Dept Mech Engn Beijing 100084 Peoples R China

In order to study the thermal stresses at the mushy zone, a three dimensional rheological model [H]-[H\N]-[N\S] including elastic, visco-elastic and visco-plastic elements was established, its algorithm and codes for finite element method were also developed. A FDM/FEM integrated analysis system of thermal stresses was built based on the integration of this procedure with thermal analysis by finite difference method. A steel sample casting with a hot spot was simulated in order to validate this system, and the calculated and experimental results were in good agreement.

关键词： Alloys