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Hierarchical learning of gastric cancer molecular subtypes by integrating multi-modal DNA-level omics data and clinical stratification

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Quantitative Biology 2024年第2期12卷 182-196页

作者： Binyu Yang siying liu Jiemin Xie Xi Tang Pan Guan Yifan Zhu Xuemei liu Yunhui Xiong Zuli Yang Weiyao Li Yonghua Wang Wen Chen Qingjiao Li Li C.Xia Department of Statistics and Financial Mathematics School of MathematicsSouth China University of TechnologyGuangzhouChina School of Physics and Optoelectronics South China University of TechnologyGuangzhouChina Department of Pathology The Sixth Affiliated HospitalSun Yat-sen UniversityGuangzhouChina School of Food Science and Engineering South China University of TechnologyGuangzhouChina Department of Laboratory Medicine the Eighth Affiliated HospitalSun Yat-sen UniversityShenzhenChina

Molecular subtyping of gastric cancer(GC)aims to comprehend its genetic ***,the efficacy of current subtyping methods is hampered by their mixed use of molecular features,a lack of strategy optimization,and the limited availability of public GC *** is a pressing need for a precise and easily adoptable subtyping approach for early DNA-based screening and *** on TCGA subtypes,we developed a novel DNA-based hierarchical classifier for gastric cancer molecular subtyping(HCG),which employs gene mutations,copy number aberrations,and methylation patterns as *** incorporating the closely related esophageal adenocarcinomas dataset,we expanded the TCGA GC dataset for the training and testing of HCG(n=453).The optimization of HCG was achieved through three hierarchical strategies using Lasso-Logistic regression,evaluated by their overall the area under receiver operating characteristic curve(auROC),accuracy,F1 score,the area under precision-recall curve(auPRC)and their capability for clinical stratification using multivariate survival ***-specific DNA alteration biomarkers were discerned through difference tests based on HCG defined *** HCG classifier demonstrated superior performance in terms of overall auROC(0.95),accuracy(0.88),F1 score(0.87)and auPRC(0.86),significantly improving the clinical stratification of patients(overall p-value=0.032).Difference tests identified 25 subtype-specific DNA alterations,including a high mutation rate in the SYNE1,ITGB4,and COL22A1 genes for the MSI subtype,and hypermethylation of ALS2CL,KIAA0406,and RPRD1B genes for the EBV *** is an accurate and robust classifier for DNA-based GC molecular subtyping with highly predictive clinical stratification *** training and test datasets,along with the analysis programs of HCG,are accessible on the GitHub website(***/LabxSCUT).

关键词： DNA alterations gastric cancer hierarchical classification molecular subtyping multi-omics

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使用Bjork-Jarabak分析法研究腺样体肥大对不同发育阶段儿童颅颌面生长趋势的影响

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实用口腔医学杂志 2024年第3期40卷 385-389页

作者：李成汉刘文林盛曾炎刘思颖口颌系统重建与再生全国重点实验室国家口腔疾病临床医学研究中心陕西省口腔疾病临床医学研究中心空军军医大学第三附属医院口腔正畸科西安710032

目的:探究腺样体肥大在不同生长发育阶段对儿童颅颌面硬组织生长发育趋势的影响。方法:对232名年龄在4~16岁儿童的头侧位片进行Bjork-Jarabak头影测量分析,采用颈椎骨龄(CVM)分期将患者分为CVM 1~2(A)、CVM 3~4(B)、CVM 5~6(C)3组,以A/N... 详细信息

目的:探究腺样体肥大在不同生长发育阶段对儿童颅颌面硬组织生长发育趋势的影响。方法:对232名年龄在4~16岁儿童的头侧位片进行Bjork-Jarabak头影测量分析,采用颈椎骨龄(CVM)分期将患者分为CVM 1~2(A)、CVM 3~4(B)、CVM 5~6(C)3组,以A/N=0.61为界设置腺样体肥大组和正常组两亚组,用t检验探究同CVM期各亚组间生长发育指标差异,以及不同CVM期该差异的变化趋势。结果:腺样体肥大儿童A、B、C组各28、55、23例,正常对照各12、65、49例。肥大组CVM1-2期S-Ar、N-Me和S-Ar/Ar-Go显著增大(P0.05)。结论:腺样体肥大对儿童颅颌面生长型影响较大,表现为髁突后位、下颌支后仰,下颌垂直向生长和顺时针旋转的趋势增强,倾向于形成Ⅱ类高角面型。此影响在生长发育早期和晚期的儿童中更为显著。

关键词：腺样体肥大错[牙合]畸形颈椎骨龄分期生长和发育

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本科实习护生循证实践能力与实习医院循证文化的相关性研究

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中华医学教育探索杂志 2024年第7期23卷 951-957页

作者：胡双刘思颖胡中豪陈文俊陈嘉张希中南大学湘雅护理学院长沙长沙医学院护理学院长沙湖南省妇幼保健院长沙

目的调查本科实习护生循证实践能力与实习医院的循证文化水平并分析两者之间的相关性, 以期为未来本科护生实习方案中循证实践环节的设置提供参考。方法 2021年12月至2022年2月, 以湖南省本科实习护生作为研究对象。采用护生循证实践能... 详细信息

目的调查本科实习护生循证实践能力与实习医院的循证文化水平并分析两者之间的相关性, 以期为未来本科护生实习方案中循证实践环节的设置提供参考。方法 2021年12月至2022年2月, 以湖南省本科实习护生作为研究对象。采用护生循证实践能力量表和医院循证文化量表对其循证实践能力和实习医院循证文化进行调查。使用SPSS 26.0进行数据的统计描述和推断。结果护生循证实践能力总得分为(84.67±27.17), 医院循证文化总得分为(79.65±15.92), 循证实践能力与医院循证文化总分及各维度均呈正相关, 相关系数为0.635～0.813, P<0.01。结论本科实习护生循证实践能力有待提升, 与医院循证文化呈正相关。学校必须与教学医院合作, 营造良好的医院循证文化氛围, 系统地优化护生的临床实习安排, 促进护生循证实践能力的发展。

关键词：本科护生循证实践循证文化横断面研究

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新型非粮蛋白源黄粉虫粉的营养特性及替代鱼粉对水产养殖动物的影响

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饲料工业 2024年第2期45卷 13-18页

作者：孟思彤王吟涛李良李登来刘思影吴莉芳吉林农业大学动物科学技术学院吉林长春130118 动物生产及产品质量安全教育部重点实验室吉林长春130118 吉林省动物营养与饲料科学重点实验室吉林长春130118

鱼粉供应资源短缺,价格日益高涨,严重制约水产养殖业的可持续发展。我国新型非粮蛋白源资源丰富,种类繁多,具有可持续性强、营养价值高等特点,但在水产饲料中的应用并未得到充分挖掘。黄粉虫(Tenebrio molitor)具有蛋白质含量高、氨基... 详细信息

鱼粉供应资源短缺,价格日益高涨,严重制约水产养殖业的可持续发展。我国新型非粮蛋白源资源丰富,种类繁多,具有可持续性强、营养价值高等特点,但在水产饲料中的应用并未得到充分挖掘。黄粉虫(Tenebrio molitor)具有蛋白质含量高、氨基酸组成均衡等优点,其虫粉是一种颇具潜力的优质非粮蛋白源。文章对黄粉虫粉的营养特性及其替代鱼粉对水产养殖动物的生长性能、肉品质、消化功能、免疫功能和抗氧化能力的影响进行概述,旨在为合理开发和应用新型非粮蛋白源提供参考。

关键词：新型非粮蛋白源黄粉虫粉水产养殖动物替代鱼粉营养特性

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蓬子菜总黄酮基于TGF-β/Smad3和JNK信号通路对动脉硬化内皮细胞的影响研究

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现代中西医结合杂志 2024年第11期33卷 1515-1519,1525页

作者：徐丹刘思赢彭湃匡东旭夏联恒许贵军韩爽黑龙江中医药大学附属第二医院黑龙江哈尔滨150000 黑龙江中医药大学附属第一医院黑龙江哈尔滨150040

目的探讨蓬子菜总黄酮基于转化生长因子-β(TGF-β)/Smad3和c-Jun氨基末端激酶(JNK)信号通路对动脉硬化内皮细胞的影响。方法将24只成年雄兔随机分为正常组、模型组、蓬子菜总黄酮低剂量组、蓬子菜总黄酮高剂量组,每组6只。除正常组外,... 详细信息

目的探讨蓬子菜总黄酮基于转化生长因子-β(TGF-β)/Smad3和c-Jun氨基末端激酶(JNK)信号通路对动脉硬化内皮细胞的影响。方法将24只成年雄兔随机分为正常组、模型组、蓬子菜总黄酮低剂量组、蓬子菜总黄酮高剂量组,每组6只。除正常组外,其余组兔建立下肢动脉硬化闭塞模型。建模成功后,蓬子菜总黄酮低、高剂量组分别给予45 mg/kg、180 mg/kg的蓬子菜总黄酮灌胃,正常组和模型组给予等量生理盐水灌胃。各组兔灌胃2周后处死,取右侧髂股动脉,分离培养内皮细胞。采用Western blot法检测内皮细胞中TGF-β_(1)、Smad3、JNK及磷酸化JNK(p-JNK)蛋白表达情况,分别采用酶联免疫分析法、硝酸还原法、放射免疫法检测内皮细胞中炎症因子[C反应蛋白(CRP)、可溶性细胞间黏附因子-1(sICAM-1)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)]、一氧化氮(NO)、内皮素-1(ET-1)含量。结果模型组内皮细胞中TGF-β_(1)、Smad3、JNK、p-JNK蛋白表达量及CRP、sICAM-1、TNF-α、IL-1β、ET-1含量均明显高于正常组(P均<0.05),蓬子菜总黄酮各组上述各指标均明显低于模型组(P均<0.05),且蓬子菜总黄酮高剂量组各指标均明显低于蓬子菜总黄酮低剂量组(P均<0.05)。模型组内皮细胞中NO含量明显低于正常组(P<0.05),蓬子菜总黄酮各组NO含量均明显高于模型组(P均<0.05),且蓬子菜总黄酮高剂量组明显高于蓬子菜总黄酮低剂量组(P<0.05)。结论蓬子菜总黄酮可减轻炎症反应,保护血管内皮细胞,抑制平滑肌细胞增殖和细胞凋亡,改善血管内皮功能,机制可能与抑制TGF-β/Smad3和JNK信号通路有关。

关键词：转化生长因子-β Smad3 蓬子菜总黄酮内皮细胞

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Electrochemical and colorimetric dual-signal detection of Staphylococcus aureus enterotoxin B based on AuPt bimetallic nanoparticles loaded Fe-N-C single atom nanocomposite

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Food Science and Human Wellness 2024年第4期13卷 2025-2035页

作者： Huan Liang Hongcheng liu Haojian Lin Guobao Ning Xiaokang Lu siying Ma Fei liu Hui Zhao Canpeng Li School of Chemical Science and Technology Yunnan UniversityKey Laboratory of Medicinal Chemistry for Natural ResourceMinistry of EducationKunming 650091China Institute of Quality Standard and Testing Technology Yunnan Academy of Agricultural ScienceSupervision and Testing Center for Farm Product QualityMinistry of AgricultureKunming 650223China State Key Laboratory of Optoelectronic Materials and Technologies Guangdong Province Key Laboratory of Display Material and TechnologySchool of Electronics and Information TechnologySun Yat-sen UniversityGuangzhou 510275China Laboratory for Conservation and Utilization of Bio-resource Yunnan UniversityKunming 650091China

Sensitive detection of Staphylococcus aureus enterotoxin B(SEB)is of importance for preventing food poisoning from threatening human *** this work,an electrochemical and colorimetric dual-signal detection assay of SEB was *** probe(Ab2/AuPt@Fe-N-C)was bound to SEB captured by Ab1,where the Ab2/AuPt@Fe-N-C triggered methylene blue degradation and resulted in the decrease of electrochemical ***,the probe catalyzed the oxidation of 3,3’,5,5’-tetramethyl biphenyl to generate a colorimetric absorbance at 652 *** the target was captured and formed a sandwich-like complex,the color changed from colorless to *** detection by colorimetric and electrochemical methods showed a linear relationship in the concentration ranges of 0.0002-10.0000 and 0.0005-10.0000 ng/mL,with limits of detection of 0.0667 and 0.1670 pg/mL,*** dual-signal biosensor was successfully used to detect SEB in milk and water samples,which has great potential in toxin detection in food and the environment.

关键词： Staphylococcus aureus enterotoxin Electrochemical immunosensor Colorimetric assay MOF@borophene composite Dual-functional Fe-N-C signal atom catalyst

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Engineered neutrophil apoptotic bodies ameliorate myocardial infarction by promoting macrophage efferocytosis and inflammation resolution

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Bioactive Materials 2022年第3期7卷 183-197页

作者： Lili Bao Geng Dou Ran Tian Yajie Lv Feng Ding siying liu Ruifeng Zhao Lu Zhao Jun Zhou Lin Weng Yan Dong Bei Li Shiyu liu Xin Chen Yan Jin State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi International Joint Research Center for Oral Diseases Center for Tissue EngineeringSchool of StomatologyThe Fourth Military Medical UniversityXi’an710032China School of Chemical Engineering and Technology Shaanxi Key Laboratory of Energy Chemical Process IntensificationInstitute of Polymer Science in Chemical EngineeringXi’an Jiao Tong UniversityXi’an710049China Department of Dermatology Tangdu HospitalThe Fourth Military Medical UniversityXi’an710038China State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi International Joint Research Center for Oral Diseases Center for Digital DentistrySchool of StomatologyThe Fourth Military Medical UniversityXi’anShaanxi710032China State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Clinical Research Center for Oral Diseases Department of Oral and Maxillofacial SurgerySchool of StomatologyThe Fourth Military Medical UniversityXi’anShaanxi710032China

Inflammatory response plays a critical role in myocardial infarction(MI)*** neutrophil apoptosis and subsequent macrophage ingestion can result in inflammation resolution and initiate regeneration,while the therapeutic strategy that simulates and enhances this natural process has not been ***,we constructed engineered neutrophil apoptotic bodies(eNABs)to simulate natural neutrophil apoptosis,which regulated inflammation response and enhanced MI *** eNABs were fabricated by combining natural neutrophil apoptotic body membrane which has excellent inflammation-tropism and immunoregulatory properties,and mesoporous silica nanoparticles loaded with hexyl 5-aminolevulinate hydrochloride(HAL).The eNABs actively targeted to macrophages and the encapsulated HAL simultaneously initiated the biosynthesis pathway of heme to produce anti-inflammatory bilirubin after intracellular release,thereby further enhancing the anti-inflammation *** in vivo studies,the eNABs efficiently modulated inflammation responses in the infarcted region to ameliorate cardiac *** study demonstrates an effective biomimetic construction strategy to regulate macrophage functions for MI repair.

关键词： Neutrophils Apoptotic bodies Engineering Inflammation Myocardial infarction

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Clinical characteristics of 19 neonates born to mothers with COVID-19

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Frontiers of Medicine 2020年第2期14卷 193-198页

作者： Wei liu Jing Wang Wenbin Li Zhaoxian Zhou siying liu Zhihui Rong Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

The aim of this study was to investigate the clinical characteristics of neonates born to SARS-CoV-2 infected mothers and increase the current knowledge on the perinatal consequences of *** neonates were admitted to Tongji Hospital from January 31 to February 29,*** mothers were clinically diagnosed or laboratory-confirmed with *** prospectively collected and analyzed data of mothers and *** are 19 neonates included in the *** them,10 mothers were confirmed COVID-19 by positive SARS-CoV-2 RT-PCR in throat swab,and 9 mothers were clinically diagnosed with *** occurred in an isolation room and neonates were immediately separated from the mothers and isolated for at least 14 *** fetal distress was *** age of the neonates was 38.61.5 weeks,and average birth weight was 3293425 ***-CoV-2 RT-PCR in throat swab,urine,and feces of all neonates were ***-CoV-2 RT-PCR in breast milk and amniotic fluid was negative *** of the neonates developed clinical,radiologic,hematologic,or biochemical evidence of *** vertical transmission of SARS-CoV-2 and no perinatal complications in the third trimester were found in our *** delivery should occur in isolation and neonates should be separated from the infected mothers and care givers.

关键词： coronavirus disease 2019 severe acute respiratory syndrome-associated coronavirus maternal-infant infection newborn

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Atomically Precise Pd Species Accelerating CO_(2) Hydrodeoxygenation into CH_(4) with 100%Selectivity

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Precision Chemistry 2023年第9期1卷 530-537页

作者： Kai Zheng siying liu Bangwang Li Juncheng Zhu Xiaojing Zhang Mingyu Wu Li Li Shan Zhu Wenxiu liu Jun Hu Chengyuan liu Minghui Fan Ming Zuo Junfa Zhu Yang Pan Yongfu Sun Yi Xie Hefei National Research Center for Physical Sciences at Microscale National Synchrotron Radiation LaboratoryUniversity of Science and Technology of ChinaHefei 230026China State Grid Anhui Electric Power Research Institute Hefei 230601 AnhuiChina

High-rate CO_(2)-to-CH_(4)photoreduction with high selectivity is highly attractive,which is a win-win strategy for mitigating the greenhouse effect and the energy ***,the poor photocatalytic activity and low product selectivity hinder the practical *** precisely tailor the product selectivity and realize high-rate CO_(2)photoreduction,we design atomically precise Pd species supported on In_(2)O_(3)*** the synthetic 1.30Pd/In_(2)O_(3)nanosheets as an example,the aberration-correction high-angle annular dark-field scanning transmission electron microscopy image displayed the Pd species atomically dispersed on the In_(2)O_(3)*** spectra and X-ray photoelectron spectra established that the strong interaction between the Pd species and the In_(2)O_(3)substrate drove electron transfer from In to Pd species,resulting in electron-enriched Pd sites for CO_(2)*** photoemission spectroscopy demonstrated that the Pd species can tailor the conduction band edge of In_(2)O_(3)nanosheets to match the CO_(2)-to-CH_(4)pathway,instead of the CO_(2)-to-CO pathway,which theoretically accounts for the high CH_(4)***,in situ X-ray photoelectron spectroscopy unveiled that the catalytically active sites had a change from In species to Pd species over the 1.30Pd/In_(2)O_(3)*** situ FTIR and EPR spectra reveal the atomically precise Pd species with rich electrons prefer to adsorb the electrophilic protons for accelerating the*COOH intermediates hydrogenation into CH_(4).Consequently,the 1.30Pd/In_(2)O_(3)nanosheets reached CO_(2)-to-CH_(4)photoconversion with 100%selectivity and 81.2μmol g^(−1)h^(−1)productivity.

关键词： CO_(2)-to-CH_(4)pathway atomically precise Pd species conduction band edge CH4 selectivity photoelectrons transfer

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Immobilization of heparin on decellularized kidney scaffold to construct microenvironment for antithrombosis and inducing reendothelialization

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Science China(Life Sciences) 2018年第10期61卷 1168-1177页

作者： Miao Wang Lili Bao Xinyu Qiu Xiaoshan Yang siying liu Yuting Su Lulu Wang Bo liu Qing He Shiyu liu Yan Jin State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases Center for Tissue Engineering School of Stomatology Fourth Military Medical UniversityXi'an 710032 China Shaanxi Institute of Medical Device Quality Supervision and Inspection Xi'an 721046 China Research and Development Center for Tissue Engineering Fourth Military Medical UniversityXi'an 710032 China Xi'an Institute of Tissue Engineering and Regenerative Medicine Xi'an 710032 China State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Clinical Research Center for Oral Diseases Department of Orthodontics School of Stomatology Fourth Military Medical UniversityXi'an 710032 China Department of aerospace Fourth Military Medical UniversityXi'an 710032 China State Key Laboratory of Military Stomatology and National Clinical Research Center for Oral Diseases and Shaanxi Clinical Research Center for Oral Diseases Department of Pediatric Dentistry School of Stomatology Fourth Military Medical UniversitXi'an 710032 China

In recent years, rapid development of tissue engineering technology provides possibilities for the construction of artificial tissues or organs. In construction of engineered kidneys, researchers used native decellularized extracellular matrix(ECM) as the scaffolds to recellularization. However, thrombosis has been a great issue that hinders the progress of transplantation in vivo. In this study, heparin was immobilized to the collagen part of decellularized scaffold with collagen-binding peptide(CBP). Through the anticoagulant and endothelial cell reperfusion experiments, it can be demonstrated that the heparinized scaffolds absorbed less platelets and red blood cells which can effectively reduce the formation of thrombosis. Moreover, it is conducive to longterm adhesion of endothelial cells which is important for the formation of subsequent vascularization. Taken together, our results reveal that the whole kidney can be modified by CBP-heparin composite to reduce the thrombosis and provide the better conditions for neovascularization.

关键词：肝磷脂微型环境构造支架肾研究人员 vivo 脚手架

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