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Truncated glycoprotein E of varicella-zoster virus is an ideal immunogen for Escherichia coli-based vaccine design

Science China(Life Sciences) 2023年第4期66卷 743-753页

作者： Tingting Chen Jie Sun Sibo Zhang Tingting Li Liqin Liu Wenhui Xue Lizhi Zhou siting liang Zhili Yu Qingbing Zheng Hai Yu Tong Cheng Jun Zhang Ying Gu Shaowei Li Ningshao Xia State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics National Institute of Diagnostics and Vaccine Development in Infectious DiseasesSchool of Life Sciences&School of Public HealthXiamen UniversityXiamen 361102China Xiang An Biomedicine Laboratory Xiamen 361102China Research Unit of Frontier Technology of Structural Vaccinology Chinese Academy of Medical SciencesXiamen 361102China

Varicella-zoster virus(VZV)is a highly infectious agent responsible for both varicella and herpes zoster *** high efficacy,there remain safety and accessibility concerns with the licensed ***,we sought to produce a VZV g E immunogen using an *** expression *** found that the soluble expression and yield of g E protein could be enhanced via C-terminal truncations to the protein,thereby facilitating a robust and scalable purification process for the purpose of vaccine *** lead truncated g E(aa 31–358),hereafter referred to as tg E,was a homogenous monomer in solution and showed excellent ***,we assessed and compared the immunogenicity of tg E with commercial v Oka LAV and Shingrix *** found that aluminum-adjuvanted tg E was immunogenic as compared with v Oka *** adjuvanted with AS01B,a two-dose immunization of tg E showed comparable or better potency in antibody responses and cell-mediated immunity with those of the Shingrix vaccine at the same dosage,especially in terms of the proportion of IFN-γ-expressing CD4^(+)T *** conclusion,this method of ***-mediate tg E expression offers a cost-effective and scalable strategy to generate an ideal VZV g E immunogen for the development of both varicella and zoster vaccines.

关键词： varicella-zoster virus glycoprotein E Escherichia coli vaccine

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虚拟现实媒介:一种新型叙事平台及其隐私挑战

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出版科学 2024年第6期32卷 5-14页

作者：梁斯婷徐丽芳武汉大学信息管理学院武汉430072

探索虚拟现实媒介沉浸性、交互性和想象力三大特性在叙事中的表现,阐明其对传统叙事方式的革新性影响。揭示在身临其境的创新体验背后,隐私挑战日益突出,需要解决的隐私问题包括:数据泄露可能导致用户信息被不必要地泄露,心理操控可能... 详细信息

探索虚拟现实媒介沉浸性、交互性和想象力三大特性在叙事中的表现,阐明其对传统叙事方式的革新性影响。揭示在身临其境的创新体验背后,隐私挑战日益突出,需要解决的隐私问题包括:数据泄露可能导致用户信息被不必要地泄露,心理操控可能导致心理伤害,空间隐私的侵犯可能削弱用户自主权等。最后,从技术、伦理和法律的角度深入讨论隐私保护的可能路径,以期为虚拟现实媒介中平衡用户体验和隐私安全的叙事实践提供参考。

关键词：虚拟现实媒介数据隐私数字叙事数字出版新媒体

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HVDC电缆电场分布影响因素的仿真研究

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中国电机工程学报 2016年第9期36卷 2563-2571页

作者：李忠华刘乐乐郑欢梁斯婷工程电介质及其应用技术教育部重点实验室(哈尔滨理工大学) 黑龙江省哈尔滨市150080

电场分布是决定电缆绝缘短时耐压能力和长期运行可靠性的关键因素。HVDC电缆稳定运行时,绝缘材料的电导率决定电场分布,在较高温度梯度分布下有可能出现电场分布翻转现象。由于电缆绝缘材料的非线性电导率是温度及电场的函数,在投入运... 详细信息

电场分布是决定电缆绝缘短时耐压能力和长期运行可靠性的关键因素。HVDC电缆稳定运行时,绝缘材料的电导率决定电场分布,在较高温度梯度分布下有可能出现电场分布翻转现象。由于电缆绝缘材料的非线性电导率是温度及电场的函数,在投入运行和电压极性反转时HVDC电缆暂态电场分布更为复杂。为此,采用多物理场耦合软件仿真研究了绝缘材料非线性电导属性对不同温度梯度、不同施压方式下电缆绝缘稳态和暂态电场的影响规律。仿真结果发现:当绝缘材料非线性属性确定,绝缘内温度梯度越高,稳态时电场分布翻转现象越严重;电压反转过程暂态电场最大值与电压极性反转时间密切相关,反转时间越短暂态最大电场越高,且暂态最大电场的位置越靠近导体屏蔽。仿真结果同时表明:降低材料电导活化能和提高材料电导率对电场依赖性有利于在温度梯度下对电缆绝缘稳态和暂态电场分布的控制。根据仿真研究结果,建议在HVDC电缆料研发时应采取有效的调控手段降低材料电导活化能和提高场致增强型电导的场强依赖系数;而在HVDC电缆设计时,要特别关注温度梯度效应和极性反转过程中的暂态电场分布问题。

关键词： HVDC电缆温度梯度暂态电场非线性电导率

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浅谈民办高校就业指导课存在的问题与解决措施

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科教导刊 2014年第7S期 13-14页

作者：梁思婷广东财经大学华商学院广东广州511300

随着高校毕业生就业形势的日趋严峻,许多民办高校也相应开设了就业指导课,这对提升民办高校学生的就业竞争力起了一定作用。然而在课程的实施中,依然存在一些问题。若要解决这些问题,应根据民办高校学生的就业特点,通过完善课程体系、... 详细信息

随着高校毕业生就业形势的日趋严峻,许多民办高校也相应开设了就业指导课,这对提升民办高校学生的就业竞争力起了一定作用。然而在课程的实施中,依然存在一些问题。若要解决这些问题,应根据民办高校学生的就业特点,通过完善课程体系、课程设置须系统化和全程化、重视专业化和职业化教师队伍的建设、丰富授课内容和形式、加强实践教学等几方面来提升民办高校就业指导课的教学质量。

关键词：民办高校就业特点就业指导问题与对策

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固定化铜绿假单胞菌GF31对氯氰菊酯降解的强化作用

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化工学报 2013年第6期64卷 2219-2226页

作者：李青云周茂钟刘幽燕唐爱星覃益民梁思婷韦滢军广西大学化学化工学院广西南宁530004 广西生物炼制重点实验室广西南宁530003 广西植保总站广西南宁530022

研究了沸石固定化铜绿假单胞菌GF31的氯氰菊酯降解特性及强化修复效果。综合考虑氯氰菊酯特性及其传质等因素,选择沸石为载体进行固定化,电镜扫描结果显示,菌株GF31能较好地生长于沸石载体孔隙中。固定化后菌株GF31的去除率和比降解速... 详细信息

研究了沸石固定化铜绿假单胞菌GF31的氯氰菊酯降解特性及强化修复效果。综合考虑氯氰菊酯特性及其传质等因素,选择沸石为载体进行固定化,电镜扫描结果显示,菌株GF31能较好地生长于沸石载体孔隙中。固定化后菌株GF31的去除率和比降解速率提高,6d对100mg·L-1氯氰菊酯的去除率由37%提高至61.4%,游离细胞和固定化细胞对于300mg·L-1的氯氰菊酯的平均比降解速率分别为487和917mg·[L·d·(g细胞干重)]-1。固定化细胞对pH和高浓度氯氰菊酯的耐受性显著增强,在pH 10或者菊酯浓度为800mg·L-1时仍能保持一定的降解活力。投加固定化细胞对实际土壤进行生物强化修复,其降解过程可用一级动力学方程来描述。与自然降解相比,氯氰菊酯的半衰期t1/2由34.3d缩短至8.02d,显示出固定化细胞良好的强化修复效果。

关键词：氯氰菊酯固定化细胞生物降解铜绿假单胞菌强化作用

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Design and Analysis of Wide Speed Regulation of Variable Leakage Flux Reverse Salient-pole Motor

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CES Transactions on Electrical Machines and Systems 2023年第3期7卷 284-293页

作者： Xiping Liu siting Zhu Dabin Liu Jianwei liang Jiangxi University of Science and Technology Ganzhou 341000China

In this article, a new variable leakage flux reverse salient-pole motor(VLF-RSPM) is raised to widen the speed range. The innovation is to realize both reverse salient-pole characteristics and variable leakage flux characteristics by using a method of adding magnetic bridges and magnetic barriers. Firstly, the evolution of the topological structure and working principle of the motor are introduced. Secondly, based on 2D Finite Element Analysis(FEA), the electromagnetic properties and noise of the motor are analyzed in detail, and the electromagnetic properties are contrasted with that of the conventional V-type synchronous motor(CVTSM). The results show that VLF-RSPM has the advantages of small torque ripple, strong magnetic weakening ability, low noise, high efficiency, and low risk of permanent magnet demagnetization under different conditions. In addition, it is verified that the proposed motor extends the speed range.

关键词： Reverse salient-pole Variable leakage flux FEA Wide speed range

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阿立哌唑与利培酮治疗首发精神分裂症的效果及对血脂代谢的影响

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数理医药学杂志 2022年第9期35卷 1335-1338页

作者：罗端庆赵斌斌吕杏放梁碧丹陈燕莉梁思挺梁畅肇庆市第三人民医院药学部肇庆526020 肇庆市第三人民医院心身科肇庆526020 肇庆市第三人民医院临床药学科肇庆526020

目的:探讨阿立哌唑与利培酮治疗首发精神分裂症(SCH)患者的效果及对血脂代谢的影响。方法:选取2019年10月-2020年10月在某院确诊的60例首发SCH患者,采用简单随机分组法将患者分为阿立哌唑组和利培酮组各30例,阿立哌唑组采用阿立哌唑口... 详细信息

目的:探讨阿立哌唑与利培酮治疗首发精神分裂症(SCH)患者的效果及对血脂代谢的影响。方法:选取2019年10月-2020年10月在某院确诊的60例首发SCH患者,采用简单随机分组法将患者分为阿立哌唑组和利培酮组各30例,阿立哌唑组采用阿立哌唑口服治疗,利培酮组采用利培酮口服治疗,均持续治疗8周。分别于治疗前后,比较两组的PANSS评分、体重、血脂代谢相关指标和治疗期间的不良反应发生情况。结果:两组总有效率无显著差异(P>0.05)。两组治疗前各项PANSS评分、体重、TC、TG、HDL和LDL无统计学差异(P>0.05);治疗后,两组各项PANSS评分均明显降低(P0.05),利培酮组体重较治疗前明显增加(P0.05),利培酮组TC和LDL较治疗前明显增加,且明显高于阿立哌唑组(P<0.05)。阿立哌唑组总不良反应发生率(13.33%)明显低于利培酮组(40.00%)(P<0.05)。结论:阿立哌唑治疗首发SCH患者的效果良好,对患者血脂代谢影响小,用药安全性高于利培酮,可优先选择阿立哌唑治疗SCH患者。

关键词：首发精神分裂症利培酮阿立哌唑血脂代谢

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Investigation of an Intensifying-flux Variable Flux-leakage Interior Permanent Magnet Machine for Wide Speed Range

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CES Transactions on Electrical Machines and Systems 2022年第2期6卷 207-215页

作者： Xiping Liu Dabin Liu siting Zhu Jianwei liang School of Electrical Engineering and Automation Jiangxi University of science and technologyGanzhou 34100China

In this paper,a novel intensifying-flux variable flux-leakage interior permanent magnet(IFVF-IPM)machine is proposed,in which flux barriers were designed deliberately between the adjacent poles to obtain intensifying-flux effect and variable flux-leakage *** rotor topology and design principles of the proposed machine are also ***,a multi-objective optimization method is adopted based on the sensitivity analysis,and some design variables of IFVF-IPM machine with strong sensitivity are selected to optimization progress by using the non-dominated sorting genetic algorithm-Ⅱ(NSGA-Ⅱ).Moreover,the electromagnetic characteristics of conventional IPM machine,conventional IFVF-IPM machine(CIFVF-IPM)and the novel IFVF-IPM machine are compared based on the finite element analysis(FEA)method which includes flux linkage,inductances characteristic,torque-speed envelops and power characteristic,as well as evaluation of the risk of irreversible ***,the experiment results show that the IFVF-IPM machine has a better performance in flux weakening capability for wide speed range and a lower risk of irreversible demagnetization,which indicates the validity and feasibility of the proposed machine.

关键词： Interior permanent magnet(IPM)machine Intensifying-flux Variable flux-leakage Electromagnetic performance Demagnetization Finite element analysis(FEA) Wide speed range

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Dynamic Landscapes of tRNA Transcriptomes and Translatomes in Diverse Mouse Tissues

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Genomics, Proteomics & Bioinformatics 2023年第4期21卷 834-849页

作者： Peng Yu siting Zhou Yan Gao Yu liang Wenbing Guo Dan Ohtan Wang Shuaiwen Ding Shuibin Lin Jinkai Wang Yixian Cun Center for Translational Medicine Precision Medicine InstituteThe First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080China Department of Radiation Oncology Affiliated Cancer Hospital&Institute of Guangzhou Medical UniversityGuangzhou 510080China Department of Medical Informatics Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China Center for Stem Cell Biology and Tissue Engineering MOE Key Laboratory for Stem Cells and Tissue EngineeringSun Yat-sen UniversityGuangzhou 510080China RIKEN Center for Biosystems Dynamics Research Kobe 650-0047Japan Graduate School of Biostudies Kyoto UniversityKyoto 606-8501Japan Wuya College of Innovation Shenyang Pharmaceutical UniversityShenyang 110016China RNA Biomedical Institute Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510080China

Although the function of tRNAs in the translational process is well established,it remains controversial whether tRNA abundance is tightly associated with translational efficiency(TE)in ***,how critically the expression of tRNAs contributes to the establishment of tissue-specific proteomes in mammals has not been well ***,we measured both tRNA expression using demethylase-tRNA sequencing(DM-tRNA-seq)and TE of mRNAs using ribosome-tagging sequencing(RiboTag-seq)in the brain,heart,and testis of *** variation in the expression of tRNA isodecoders was observed among different *** the statistical effect of isodecoder-grouping on reducing variations is considered through permutating the anticodons,we observed an expected reduction in the variation of anticodon expression across all samples,an unexpected smaller variation of anticodon usage bias,and an unexpected larger variation of tRNA isotype expression at amino acid *** of whether or not they share the same anticodons,the isodecoders encoding the same amino acids are co-expressed across different *** on the expression of tRNAs and the TE of mRNAs,we find that the tRNA adaptation index(tAI)and TE are significantly correlated in the same tissues but not between tissues;and tRNA expression and the amino acid composition of translating peptides are positively correlated in the same tissues but not between *** therefore hypothesize that the tissue-specific expression of tRNAs might be due to post-transcriptional *** study provides a resource for tRNA and translation studies,as well as novel insights into the dynamics of tRNAs and their roles in translational regulation.

关键词： Translational efficiency Tissue-specific tRNA expression Codon usage bias Amino acid composition

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Depression accelerates gastric cancer invasion and metastasis by inducing a neuroendocrine phenotype via the catecholamine/β_(2)-AR/MACC1 axis

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Cancer Communications 2021年第10期41卷 1049-1070页

作者： Changqie Pan Jianhua Wu siting Zheng Huiying Sun Yisheng Fang Zhenhua Huang Min Shi Li liang Jianping Bin Yulin Liao Jinzhang Chen Wangjun Liao Department of Oncology Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdong 510515P.R.China Department of Pathology Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdong 510515P.R.China Department of Cardiology Nanfang HospitalSout4hern Medical UniversityGuangzhouGuangdong 510515P.R.China

Background:Depression is a common,easily ignored,accompanied disease of gastric cancer(GC)patients and is often observed with elevated plasma catecholamine *** frequently promotes GC progression and leads to poor clinical outcomes;however,the molecular mechanisms underlying depression-induced GC progression remain poorly *** aimed to study the effects of depression on GC progression and explore possible mechanisms mediating the action of depression-associated catecholamines on ***:Depression states of GC patients were graded using the Patient Health Questionnaire-9,and plasma catecholamine levels were examined by high performance liquid chromatography coupled with tandem mass *** and invasive GC cells were examined using transwell assays,and metastatic GC niches were imaged using bioluminescence technology in a depression mouse model established with chronic unpredictable mild *** depression-like behaviors were assessed through sucrose preference,forced swimming,and tail suspension *** of the neuroendocrine phenotype were observed via RT-PCR,Western blotting,flow cytometry,and transmission electron ***:Fifty-one GC patients(age:53.61±1.79 years;cancer duration:3.71±0.33 months;depression duration:2.37±0.38 months;male-to-female ratio:1.55:1)were enrolled in the *** grade was significantly higher in GC patients showing higher plasma levels of catecholamines(epinephrine:P=0.018;noradrenaline:P=0.009),higher oncogene metastasis-associated in colon cancer-1(MACC1)level(P=0.018),and metastasis(P<0.001).Further,depression-associated catecholamine specifically bound to the beta-2 adrenergic receptor(β_(2)-AR)and upregulated MACC1 expression,and thus promoting neuroendocrine phenotypic transformation through direct binding betweenMACC1 and ***,the neuroendocrine phenotypic transformation accelerated GC invasion in vitro and metastasis in ***

关键词： catecholamines depression gastric cancer MACC1 neuroendocrine phenotype psychological stress tumor metastasis

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