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Application of Zinc, Iron and Boron Enhances Productivity and Grain Biofortification of Mungbean

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Phyton-International Journal of Experimental Botany 2023年第4期92卷 983-999页

作者： Muhammad Zafar siraj ahmed Muhammad Kashif Munir Nawal Zafar Muhammad Saqib Muhammad Aleem Sarwar Saba Iqbal Baber Ali Naveed Akhtar Basharat Ali Sadam Hussain Muhammad Saeed Mohammad Khalid Al-Sadoon Aneela Gulnaz Agronomic Research Institute Ayub Agricultural Research Institute FaisalabadFaisalabad38000Pakistan Soil and Water Testing Laboratory Ayub Agricultural Research Institute FaisalabadFaisalabad38000Pakistan Department of Plant Sciences Quaid-i-Azam UniversityIslamabad45320Pakistan Department of Agricultural Engineering Khwaja Fareed University of Engineering and TechnologyRahim Yar Khan64200Pakistan College of Agronomy Northwest A&F UniversityYangling712100China Plant Pathology Research Institute Faisalabad38000Pakistan Department of Zoology College of ScienceKing Saud UniversityRiyadh11451Saudi Arabia Department of Biotechnology Yeungnam UniversityGyeongsan38641Korea

Deficiencies of essential vitamins,iron(Fe),and zinc(Zn)affect over one-half of the world’s population.A significant progress has been made to control micronutrient deficiencies through supplementation,but new approaches are needed,especially to reach the rural *** biofortification of pulses with Zn,Fe,and boron(B)offers a pragmatic solution to combat hidden hunger instead of food fortification and ***,it also has positive effects on crop production as ***,we conducted three separate field experiments for two consecutive years to evaluate the impact of soil and foliar application of the aforementioned nutrients on the yield and seed biofortification of *** application of Zn at 0,4.125,8.25,Fe at 0,2.5,5.0 and B at 0,0.55,1.1 kg ha−1 was done in the first,second and third experiment,*** application in these experiments was done at 0.3%Zn,0.2%Fe and 0.1%B respectively one week after flowering *** revealed that soil-applied Zn,Fe and B at 8.25,5.0 and 1.1 kg ha−1,respectively,enhanced the grain yield of mungbean;however,this increase in yield was statistically similar to that recorded with Zn,Fe and B at 4.125,2.5 and 0.55 kg ha−1,*** application of these nutrients at flower initiation significantly enhanced the Zn contents by 28%and 31%,Fe contents by 80%and 78%,while B contents by 98%and 116%over control during 2019 and 2020,*** was concluded from the results that soil application of Zn,Fe,and B enhanced the yield performance of mungbean;while significant improvements in seed Zn,Fe,and B contents were recorded with foliar application of these nutrients.

关键词： Biofortification seed biofortification mungbean iron zinc boron foliar application

Immunogenic potential and protective efficacy of formalin inactivated circulating Indian strain of West Nile virus

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Asian Pacific Journal of Tropical Medicine 2014年第12期7卷 946-951页

作者： Pritom Chowdhury siraj ahmed Khan Rashmee Topno Prafulla Dutta R.N.S.Yadav Jagadish Mahanta Regional Medical Research Centre ICMR(NE Region)DibrugarhAssamIndia Centre for studies in Biotechnology Dibrugarh UniversityDibrugarh-786004AssamIndia

Objective:To assess the best suitable condition for virus *** to study the immunogenic potential and protective efficacy of a circulating West Nile virus(WNV) strain in ***:Bulk preparation of circulating WNV:WNIRGC07(GeneBank ID:HQ246154).was undertaken in a bioreaclor using *** Inactivation was done in three different conditions:22 ℃.4 ℃ and room *** virus preparations were evaluated for antigenicity by ELISA and toxicity by cell proliferation *** efficacy was done in-viro on swiss albino mice against standard Indian WNV and Japanese encephalitis virus(JKV)*** and cell mediated immune response was evaluated in mice sera by ELISA and neutralization ***:Inactivation at 22 ℃ was found to be more suitable in terms of less toxicity and high *** same was selected to study the immune response and efficacy in *** induced neutralizing antibody titre of 1:625 and high EgG *** vivo experiment showed 100% protective efficacy against WNV and 20.8% cross protective efficacy against *** assessment of cellular immunity through immunized mice revealed augmentation of high levels of pro-inflammatory cytokines and moderate levels of anti—cytokines indicating a mixed balance of Th1 and Th2 ***:Findings suggest that formalin inactivated Indian WNV strain has a good immunogenic *** is the first study on assessment of immunogenic potential of a lineage 5 strain of *** study reveals that it would be a promising and effective candidate for vaccine studies which warrants further evaluation.

关键词： West Nile virus Assam Immunization Efficacy Neutralizing antibody Cytokine

Genetic diversity and gene structure of mitochondrial region of Anopheles minimus(Diptcra:Culicidae)-major malaria vector of North east India

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Asian Pacific Journal of Tropical Medicine 2014年第12期7卷 952-955页

作者： Prafulla Dutta siraj ahmed Khan Rashmee Topno Pritom Chowdhury Mayuri Baishya Purvita Chowdhury Jagadish Mahanta Regional Medical Research Centre ICMR(NE Region)DibrugarhAssamIndia

Objective:To depict mitochondrial genetic variation for the first time among Anopheles minimus(***)(Diptera:Culicidae) species from two malaria endemic states of NE ***:Phylogeographic analysis was carried at 9 out of 12 sites oi *** confirmed malaria endemic ***:All sequences were Adenine-Thymine rich *** were observed in 6 sequences where 5 mutations were synonymous substitutions and in 1 ease non synonymous mutation was *** distinct clusters of haplolypes were *** diversity and low nucleotide diversity were *** negative values obtained from Tajima's D test and Fu'sF_S test indicate a recent genetic population *** analysis has explained sequence diversity that was also shown by mutations in 6 ***:High genetic diversity observed within the populations of *** species has several possible implications for vector control in the region.

关键词： Anopheles minimus Cytochrome oxidase Ⅱ Haplotype Mutation Substitution

Evaluating the Optimum Transplanting Time for Different Coarse Rice Genotypes under Semi-Arid Conditions of Faisalabad

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Agricultural Sciences 2018年第1期9卷 69-77页

作者： Muhammad Ahtisham Tahir Muhammad Ahmad Arain Saba Durrani Abdul Shakoor Ahtsham Bilal Nasir Ali Muhammad Ishfaq Umer Farooq siraj ahmed Muhammad Irfan Department of Agronomy University of Agriculture Faisalabad Pakistan Department of Botany University of Agriculture Faisalabad Pakistan Department of Plant Breeding and Genetics University of Agriculture Faisalabad Pakistan Department of Plant Breeding and Genetics Bahauddin Zakariya University Multan Pakistan

Continuously changing climate and availability of different rice genotypes make it necessary to find optimum time of sowing as well as suitable variety for cultivation to get maximum productivity under a specific set of climatic conditions. A field study was carried out to search out the suitable rice transplanting time for four different coarse genotypes under the semi-arid environment of Faisalabad. The experiment was conducted at Agronomic Research Area, University of Agriculture, Faisalabad and was laid out in randomized complete block design (RCBD) with split plot arrangement keeping transplanting time in main plots while rice genotypes in subplots. Variability among treatments was measured by Fisher’s ANOVA (P ≤ 5%) and LSD test was applied to compare the differences among treatments’ means. The ANOVA indicated statistically significant differences among genotypes as well as transplanting dates irrespective of all studied traits while interactive effects of both were found to be non-significant. NIBGE-1 performed best with maximum paddy yield of 6.05 t/ha while KSK-434 performed poor with paddy yield of 2.78 t/ha. Increased paddy yield and yield related parameters of all rice genotypes were recorded where transplantation was done on 25th of June. Generally, paddy yield decreased with delaying the transplanting time. The results suggested that NIBGE-1 can perform better under the semi-arid conditions of Faisalabad and last week of June might be the optimum time for nursery transplantation. It can also be further elucidated that late transplanting causes yield reduction which could not be recommended among farmers.

关键词： Coarse Rice Genotypes Transplanting Time Paddy Yield Semi-Arid

National guidelines for the diagnosis and treatment of hilar cholangiocarcinoma

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World Journal of Gastroenterology 2024年第9期30卷 1018-1042页

作者： Faisal Saud Dar Zaigham Abbas Irfan ahmed Muhammad Atique Usman Iqbal Aujla Muhammad Azeemuddin Zeba Aziz Abu Bakar Hafeez Bhatti Tariq Ali Bangash Amna Subhan Butt Osama Tariq Butt Abdul Wahab Dogar Javed Iqbal Farooqi Faisal Hanif Jahanzaib Haider siraj Haider Syed Mujahid Hassan Adnan Abdul Jabbar Aman Nawaz Khan Muhammad Shoaib Khan Muhammad Yasir Khan Amer Latif Nasir Hassan Luck Ahmad Karim Malik Kamran Rashid Sohail Rashid Mohammad Salih Abdullah Saeed Amjad Salamat Ghias-un-Nabi Tayyab Aasim Yusuf Haseeb Haider Zia Ammara Naveed Department of Hepatopancreatic Biliary Surgery&Liver Transplant Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Hepatogastroenterology and Liver Transplantation Dr.Ziauddin University HospitalKarachi 75600SindhPakistan University of Aberdeen Aberdeen B243FXUnited Kingdom Department of Pathology Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Gastroenterology&Hepatology Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Radiology Aga Khan UniversityKarachi 74800Pakistan Department of Oncology Hameed Latif HospitalLahore 54000Pakistan Division of Hepatopancreatic Biliary Surgery&Liver Transplantation Shifa International HospitalIslamabad 44000Pakistan Department of Hepatopancreatic Biliary Surgery&Liver Transplant Shaikh Zayed Hospital and Post Graduate Medical InstituteLahore 54000Pakistan Department of Medicine Aga Khan University HospitalKarachi 74800Pakistan Department of Liver Transplant Pir Abdul Qadir Shah Jeelani Institute of Medical SciencesGambat 66020Pakistan Department of Medicine&Gastroenterology Lifecare Hospital and Research CentrePeshawar 25000Khyber PakhtunkhwaPakistan Department of Hepatopancreatobiliary&Liver Transplant Bahria International HospitalLahore 54000Pakistan Department of Surgery Hepatopancreatobiliary&Liver TransplantDow University of Health SciencesKarachi 74800Pakistan Department of Gastroenterology Hepatology&NutritionPir Abdul Qadir Shah Jeelani Institute of Medical SciencesGambat 66020Pakistan Department of Oncology Dr.Ziauddin HospitalKarachi 74800Pakistan Department of Radiology Rehman Medical InstitutePeshawar 25000Pakistan Army Liver Transplant Unit Pak Emirates Military HospitalRawalpindi 46000Pakistan Department of Gastroenterology Sindh Institute of Urology and TransplantationKarachi 75500Pakistan Rashid Nursing Home and Cancer Clinic Rashid Nursing Home and Cancer ClinicRawalpindi 46000Pakistan Department of Gastroentero

A consensus meeting of national experts from all major national hepatobiliary centres in the country was held on May 26,2023,at the Pakistan Kidney and Liver Institute&Research Centre(PKLI&RC)after initial consultations with the *** Pakistan Society for the Study of Liver Diseases(PSSLD)and PKLI&RC jointly organised this *** effort was based on a comprehensive literature review to establish national practice guidelines for hilar cholangiocarcinoma(hCCA).The consensus was that hCCA is a complex disease and requires a multidisciplinary team approach to best manage these *** coordinated effort can minimise delays and give patients a chance for curative treatment and effective *** diagnostic and staging workup includes high-quality computed tomography,magnetic resonance imaging,and magnetic resonance *** cytology or biopsy utilizing endoscopic retrograde cholangiopancreatography is a mainstay for ***,histopathologic confirmation is not always required before *** ultrasound with fine needle aspiration of regional lymph nodes and positron emission tomography scan are valuable adjuncts for *** only curative treatment is the surgical resection of the biliary tree based on the Bismuth-Corlette *** patients with unresectable hCCA can be considered for liver *** chemotherapy should be offered to patients with a high risk of *** use of preoperative biliary drainage and the need for portal vein embolisation should be based on local multidisciplinary *** with acute cholangitis can be drained with endoscopic or percutaneous biliary *** chemotherapy with cisplatin and gemcitabine has shown improved survival in patients with irresectable and recurrent hCCA.

关键词： Hilar cholangiocarcinoma Bismuth-Corlette classification Memorial Sloan Kettering Cancer Centre Staging Preoperative biliary drainage Portal vein embolisation Surgical resection Hepatectomy

Curcumin:reclaiming the lost ground against cancer resistance

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Cancer Drug Resistance 2021年第2期4卷 298-320页

作者： siraj Shaikh Javed Shaikh Yusufi Sadia Naba Kailas Doke Khursheed ahmed Mujahid Yusufi Post-Graduate Department of Chemistry and Research Center Abeda Inamdar Senior College of ArtsScience and Commerce(Affiliated to SPPU)Pune 411001India Advanced Scientific Research Laboratory Azam CampusPune 411001India.

Curcumin, a polyphenol, has a wide range of biological properties such as anticancer, antibacterial, antitubercular, cardioprotective and neuroprotective. Moreover, the anti-proliferative activities of Curcumin have been widely studied against several types of cancers due to its ability to target multiple pathways in cancer. Although Curcumin exhibited potent anticancer activity, its clinical use is limited due to its poor water solubility and faster metabolism. Hence, there is an immense interest among researchers to develop potent, water-soluble, and metabolically stable Curcumin analogs for cancer treatment. While drug resistance remains a major problem in cancer therapy that renders current chemotherapy ineffective, curcumin has shown promise to overcome the resistance and re-sensitize cancer to chemotherapeutic drugs in many studies. In the present review, we are summarizing the role of curcumin in controlling the proliferation of drug-resistant cancers and development of curcumin-based therapeutic applications from cell culture studies up to clinical trials.

关键词： Curcumin drug-resistant cancer signaling pathways clinical trials

The Development of ZnO Nanoparticle Coated Cotton Fabrics for Antifungal and Antibacterial Applications

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Materials Sciences and Applications 2020年第9期11卷 601-610页

作者： Tanu Shree Roy siraj Ud Daula Shamim Md. Khalidur Rahman F. ahmed M. A. Gafur Department of Physics Bangladesh University of Textile Dhaka Bangladesh Department of Physics Mawlana Bhashani Science and Technology University Tangail Bangladesh Department of Electrical & Electronic Engineering Prime University Dhaka Bangladesh Department of Physics Jahangirnagar University Saver Dhaka Bangladesh PP & PDC Bangladesh Council of Scientific & Industrial Research (BCSIR) Dhaka Bangladesh

In the textile industry, cotton is the most popular choice among all the natural fibers due to its unique properties such as softness, affinity to skin, biodegradability, absorbency, and moisture being comfortable to wear. Zinc Oxide Nanoparticles (ZnO NPs) were produced and coated on cotton fabrics. The concentration of Zinc acetate was varied. 2-methoxy-ethanol has been used as a solvent. Using the dip coating technique, ZnO NPs have been coated on the cotton fabric in different mole concentrations. Scanning Electron Microscopy (SEM), Ultraviolet Visible (UV-VIS) spectroscopy, the antifungal and antibacterial activity of the ZnO NPs was performed on the coated fabrics. The SEM images depicted that the nanoparticles are well dispersed on the fabric at 3M concentration of ZnO solution. It was found that the UV absorbance increases with the increase of concentration up to 2M concentration and after that it decreases. It was also found that maximum antibacterial and antifungal activity is at 2M concentration.

关键词： Nanoparticles ZnO Cotton SEM UV-VIS Antibacterial Antifungal

Knowledge, Attitude and Practice (KAP) Assessment of Intestinal Parasitic Infection among School Children in Asmara, Eritrea

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Health 2017年第1期9卷 57-68页

作者： Khawaja Shakeel ahmed Nuredin Mohamedkassm siraj Henok Fitsumberhan Sara Isaac Semhar Yohannes Dawit Eman Yacob Berhane Matiwos Araya Department of Biomedical Sciences Asmara College of Health Sciences Asmara Eritrea

Purpose: Poor hygiene practice, inadequate sanitary conditions and lack of awareness of correct mode of transmission are some factors that attribute to high intestinal parasitic infection among children. Therefore, having proper knowledge, good attitude and practice (KAP) against the aforementioned factors can significantly mitigate the spreading of intestinal infection. Methods: The present study was a cross-sectional study which comprised of 127 students who were selected by stratified random sampling. A self-administered questionnaire was used to record KAP of students against intestinal parasitic infection. Total 126 students gave stool samples which were analysed by formol-ether concentration technique. In addition, sewage samples were also collected and analysed for any parasitic load. Statistical data were generated by Chi-square test. Results: A total of 126 stool samples were examined in the study of which 46 (36.50%) were found infected with one or more intestinal parasites. Among the parasites identified, the most common was Hymenolepsis nana with a prevalence rate of 35 (76.0%). Here, most of the students 96 (76.1%) were having poor knowledge about the correct mode of transmission of intestinal parasites. Majority of the students 91 (72.2%) practicing defecation in open air when they are at home, and all 126 (100%) are doing that at school, and significant number of students were not washing hands after defecation. Conclusion: Lack of knowledge regarding mode of transmission and practice of defecation in open air and not washing hands after defecation are issues of great concern, because many parasitic infection have faecal-oral mode of transmission. So, health authorities have to take these issues seriously and action would be vital to resolve it.

关键词： Eritrea Asmara School Children Knowledge Attitude Practice

卵巢移行细胞癌:一例报道与文献复习(英文)

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The Chinese-German Journal of Clinical Oncology 2013年第9期12卷 454-456页

作者： Sheikh Aijaz Aziz Abdul Rashid Lone Mohmad Hussain Mir Sumyra Khursheed Qadri Arif Nabi Bhat Farhana siraj Bagdadi Mir Muzaffar Bashir Sanjeed ahmed Department of Medical Oncology Sheri Kashmir Instiute of Medical Sciences Department of Pathology Sheri Kashmir Instiute of Medical Sciences Department of Internal Medicine Sheri Kashmir Instiute of Medical Sciences Department of Radio Diagnosis Sheri Kashmir Instiute of Medical Sciences

Transitional cell carcinoma(TCC)of the ovary is a rare and recently recognized subtype of ovarian epithelial *** presented the first case report from our Institute(Sheri Kashmir Instiute of Medical Sciences,Srinagar,India),which was initially misdiagnosed as stromal cell carcinoma(granulosa cell tumour),and on review of histopathology with immunohistochemistry,the diagnosis of TCC of the ovary was *** aim of this article was to describe the typical case of primary TCC of the ovary and to review the literature for information on TCC management.

关键词：卵巢颗粒细胞病例报告文献移行癌复习组织病理学克什米尔