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PET imaging of γ-Glutamyltranspeptidase activity in living animals with an activatable fluorine-18 labelled probe

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PET imaging of γ-Glutamyltranspeptidase activity in living ...

厌氧污泥释放磷的规律及鸟粪石法回收磷的应用研究

第十一届全国化学生物学学术会议

作者： Ling Qiu siqin ye Jianguo Lin Key Laboratory of Nuclear Medicine of Ministry of Health Jiangsu Key Laboratory of Molecular Nuclear MedicineJiangsu Institute of Nuclear Medicine

Objective: Multiple enzymes are usually involved in a given biological process to regulate a variety of diseases including malignancy. γ-Glutamyltranspeptidase（GGT） is a cell membrane-associated enzyme which has been recognized as a promising biomarker for the diagnosis and treatment of many malignant tumors. Thus, developing a simple method to accurately detect the GGT activity would be highly desirable to improve the diagnosis efficacy. Herein, a GGT-responsive 18 F-labeled probe 18 F-1-P was rationally designed and prepared for positron emission tomography（PET） imaging of tumors to improve the early diagnosis of diseases. A satisfactory RLY and RCP was achieved when the probe 18 F-1-P was incubated at 80℃°C for 30 min. Stability test proved that it was stable enough in PBS and serum. MTT assay showed that more than 90% of the cells survived up to 24 h at the concentration up to 100 μM of 1-P. The partition coefficient revealed that the probe 18 F-1-P was weakly hydrophilic（log P =-0.0038 ± 0.0001）. Cell uptake studies suggested that treatment of 18 F-1-P with the cold probe 1-P could accelerate the uptake in HCT116 cells, which gradually reached its plateau at 1 h（2.71 % of total radioactivity） and reached its maximum of 2.86% at 2 h. This was about 1.4 times higher than that of 18 F-1-P alone as well as 3-fold higher uptake than the negative control probe 1. microPET imaging of HCT116 xenografts clearly indicated 18 F-1-P could rapidly accumulate in the tumor when it was co-injected with the cold probe 1-P and the tumor uptake reached 4.41 ± 0.65% ID/g at 15 min post injection, whereas mice only injected with 18 F-1-P had the tumor uptake of 1.97 ± 0.17% ID/g at the same time point. A novel GGT-activatable probe 18 F-1-P was designed and developed for both in vitro and in vivo imaging of endogenous GGT activity. It showed specific tumor uptake in the tumor bearing mice and the tumor-to-muscle uptake ratio remarkably enhanced when it was co-injected with the cold

关键词： Positron emission tomography (PET) γ-Glutamyltranspeptidase (GGT) molecular probe fluorine-18 labelling

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生态环境学报 2012年第4期21卷 715-719页

作者：孙连鹏沈瑞芳黎淑端叶斯琴唐哲伍世嘉中山大学环境科学与工程学院广东广州510275 广东省环境污染控制与修复技术重点实验室广东广州510275 广州市疾病预防控制中心广东广州510090

城市污水处理厂进行脱氮除磷工艺时,大量的磷从水体转入活性污泥中,如不能回收这部分磷,将造成磷的大量流失,这与磷矿稀缺的现状形成矛盾。通过建立污泥停留时间5 d和10 d的两个厌氧反应系统,对污泥中磷的释放规律进行研究,并采用鸟粪... 详细信息

城市污水处理厂进行脱氮除磷工艺时,大量的磷从水体转入活性污泥中,如不能回收这部分磷,将造成磷的大量流失,这与磷矿稀缺的现状形成矛盾。通过建立污泥停留时间5 d和10 d的两个厌氧反应系统,对污泥中磷的释放规律进行研究,并采用鸟粪石法对上清液中的磷进行回收。研究结果表明,厌氧污泥上清液中的磷含量高达150 mg.L-1,SRT为5 d和10 d的系统,分别在4 d和2 d后磷的质量浓度由14 mg.L-1上升到100 mg.L-1,且基本上都以正磷酸盐的形式存在。将pH从8.3上升到9.0,磷的回收率可以从60%提升到90%,当pH达到8.8时,磷的回收率即可超过80%,表现出了较好的磷回收效果。研究还发现镁离子和磷酸根的摩尔比对磷的回收率的影响较小,在pH=8.8时,将镁磷比从1.43提升到1.83,磷的回收率仅从79.2%提升到85.5%;在pH=9.0时提高镁磷比对磷的回收基本没有影响。

关键词：鸟粪石厌氧污泥磷回收副反应

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基于特征提取与边缘点投票的实时车道线识别

汽车工程师 2024年第8期 29-35页

作者：杨达魏长河贾成禹叶思琴三一重工股份有限公司长沙410199 长沙理工大学长沙410114

针对低算力车载计算平台的车道线检测需求,提出了一种低算力依赖的实时车道线识别方法。考虑车辆行驶过程中的光照变化,提出一种自适应光照的颜色分离方法实现车道特征提取;基于经典的边缘检测与霍夫变换算法,定义有效边缘点形式,通过... 详细信息

针对低算力车载计算平台的车道线检测需求,提出了一种低算力依赖的实时车道线识别方法。考虑车辆行驶过程中的光照变化,提出一种自适应光照的颜色分离方法实现车道特征提取;基于经典的边缘检测与霍夫变换算法,定义有效边缘点形式,通过边缘点投票确定车道直线;利用车道直线对边缘点进行筛选与补充,应用随机抽样一致性算法获取车道曲线方程。试验验证结果表明,所提出方法在低算力处理器上的识别精度高于98%,计算速度为38帧/s,并在多种应用场景下具备稳定性与鲁棒性。

关键词：智能驾驶车道线识别边缘检测随机抽样一致性自适应光照

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烯酰吗啉对土壤呼吸及微生物多样性的影响

西北农林科技大学学报（自然科学版） 2018年第2期46卷 121-130页

作者：周东兴邓杰李晶邬欣慧荣国华苏叶斯琴毕力格东北农业大学资源与环境学院黑龙江哈尔滨150030

【目的】研究烯酰吗啉对土壤呼吸强度和微生物多样性的影响,为烯酰吗啉的正确合理应用提供指导和科学依据。【方法】称取2kg供试土壤,在土壤中施用不同含量的烯酰吗啉水溶液,使土壤中烯酰吗啉的初始含量分别为5,10和25mg/kg,以不施用烯... 详细信息

【目的】研究烯酰吗啉对土壤呼吸强度和微生物多样性的影响,为烯酰吗啉的正确合理应用提供指导和科学依据。【方法】称取2kg供试土壤,在土壤中施用不同含量的烯酰吗啉水溶液,使土壤中烯酰吗啉的初始含量分别为5,10和25mg/kg,以不施用烯酰吗啉处理为空白组(CK),采用CO_2直接吸收法测定土壤呼吸强度,采用BIOLOG ECO法测定土壤微生物群落多样性和碳源利用率(AWCD);最后采用主成分分析法分析不同含量烯酰吗啉对31种碳源的利用情况。【结果】与CK相比,烯酰吗啉对土壤呼吸强度总体有明显的抑制作用,随着烯酰吗啉含量和施用次数的增加,土壤呼吸强度受抑制程度越大。前两次施用5,10mg/kg烯酰吗啉后对土壤微生物多样性指标影响不显著;第3次施用5,10mg/kg烯酰吗啉后,随着土壤中烯酰吗啉的积累,Shannon物种丰富度指数和McIntosh指数与CK相比总体显著降低,而Simpson优势度指数无显著变化。施用1~3次,与CK相比,25mg/kg的烯酰吗啉处理土壤Shannon物种丰富度指数、Simpson优势度指数及McIntosh指数总体显著降低。在第1次施用后,低含量(5,10mg/kg)烯酰吗啉污染对土壤AWCD值影响较小,高含量(25mg/kg)烯酰吗啉对土壤AWCD值影响明显,对土壤微生物的代谢活性有明显抑制作用;在第2次和第3次施用后,与CK相比,无论是低含量还是高含量,烯酰吗啉处理土壤AWCD值总体显著减小,烯酰吗啉对土壤微生物代谢活性的抑制作用增强。施用烯酰吗啉影响了土壤微生物碳源利用方式和土壤微生物群落结构。【结论】与低含量烯酰吗啉相比,高含量烯酰吗啉对土壤呼吸的抑制作用增大,明显改变了土壤微生物群落多样性。土壤微生物对碳水化合物类碳源利用率明显增大。

关键词：烯酰吗啉土壤呼吸强度 BIOLOG ECO法微生物多样性

Corrosion and tribocorrosion resistance of MAO-based composite coating on AZ31 magnesium alloy

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Journal of Magnesium and Alloys 2022年第12期10卷 3406-3417页

作者： siqin Liu Guihua Li Yuming Qi Zhenjun Peng Yinping ye Jun Liang State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000PR China Key Laboratory of Science and Technology on Wear and Protection of Materials Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000PR China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049PR China

In this paper, a multi-functional composite coating with low friction coefficient, high wear resistance and excellent tribocorrosion resistance is fabricated on AZ31 Mg alloys by micro-arc oxidation and spray-coating methods. The microstructure, and composition of the coating are characterized by SEM, EDS, XRD, and FT-IR. Potentiodynamic polarization and EIS tests are conducted to evaluate the corrosion resistance of the composite coating. The tribocorrosion property is also studied using pin-on-disk tribometer in 3.5 wt.% Na Cl solution. It is found that the composite coating possesses better long-term corrosion resistance than the single MAO coating. The tribocorrosion tests prove that the composite coating exhibits much better wear and tribocorrosion resistance than the single MAO coating and can protect the substrate from corrosion under a sliding condition.

关键词： Magnesium alloy Micro-arc oxidation Composite coating Corrosion Tribocorrosion

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八年制临床医学专业学生模拟教学效果探析

中华医学教育杂志 2016年第4期36卷 555-558页

作者：梁书静姜冠潮张斯琴叶辉钱晓伟陈红北京大学人民医院临床能力培训中心 100044 北京大学人民医院 100044

追踪和比较北京大学人民医院八年制临床医学专业学生进入第二阶段培养即住院医师轮转期间的表现,分析探讨临床见习和实习阶段模拟教学课程改革的成效和未来改进的方向,从而就模拟教学师资队伍建设、病例教案设计、高端智能模拟人系统应... 详细信息

追踪和比较北京大学人民医院八年制临床医学专业学生进入第二阶段培养即住院医师轮转期间的表现,分析探讨临床见习和实习阶段模拟教学课程改革的成效和未来改进的方向,从而就模拟教学师资队伍建设、病例教案设计、高端智能模拟人系统应用开发等方面提出具体意见和建议.

关键词：八年制临床医学专业医学生模拟教学效果探讨

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Morphological, phenological, and transcriptional analyses provide in sight into the diverse flowering traits of a mutant of the relic woody plant Liriodendron chinense

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Horticulture Research 2021年第1期8卷 2345-2360页

作者： Yu Sheng Zhaodong Hao ye Peng siqin Liu Lingfeng Hu Yongbao Shen Jisen Shi Jinhui Chen Key Laboratory of Forest Genetics&Biotechnology of Ministry of Education Co-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjingChina College of Biology and the Environment Nanjing Forestry UniversityNanjingChina Southern Tree Seed Inspection Center National Forestry Administration Nanjing Forestry UniversityNanjingChina

Flowering is crucial to plant reproduction and controlled by multiple ***,the mechanisms underlying the regulation of flowering in perennial plants are still largely ***,we first report a super long blooming 1(slbl)mutant of the relict tree Liriodendron chinense possessing a prolonged blooming period of more than 5 months,in contrast to the 1 month blooming period in the wild type(WT).Phenotypic characterization showed that earlier maturation of lateral shoots was caused by accelerated axillary bud fate,leading to the phenotype of continuous flowering in slbl *** transcriptional activity of genes related to hormone signaling(auxin,cytokinin,and strigolactone),nutrient availability,and oxidative stress relief further indicated active outgrowth of lateral buds in slbl ***,we discovered a unique FT splicing variant with intron retention specific to slbl mutants,representing a potential causal mutation in the slbl ***,most slbl inbred offspring flowered precociously with shorter juvenility(~4 months)than that(usually 8-10 years)required in WT plants,indicating heritable variation underlying continuous flowering in slbl *** study reports an example of a perennial tree mutant that flowers continuously,providing a rare resource for both breeding and genetic research.

关键词： traits Morphological flowers

Genome-wide identification and cold stress-induced expression analysis of the CBF gene family in Liriodendron chinense

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Journal of Forestry Research 2021年第6期32卷 2531-2543页

作者： Yuanlin Guan siqin Liu Weihuang Wu Kaiyue Hong Rongxue Li Liming Zhu Yang Liu ye Lu Jinhui Chen Liming Yang Jisen Shi Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education of China Co-Innovation Center for Sustainable Forestry in Southern ChinaNanjing Forestry UniversityNanjing 210037JiangsuPeople’s Republic of China College of Biology and the Environment Nanjing Forestry UniversityNanjing 210037JiangsuPeople’s Republic of China

Cold-resistance pathways that operate in model plants such as Arabidopsis thaliana and Oryza sativa have been studied *** has been found that CBF genes play an important role in plant cold *** chinense,a tree known for its graceful tree shape and widely spread in south China,has weak cold ***,little is known about its response to *** further study the function of *** CBF gene family,we started by characterizing all members of this gene family in the *** genome and their expression *** analysis found that 14 CBF genes in *** are more closely related to their homologues in woody plants and *** than those in ***-acting elements and GO analysis showed that some LcCBF genes participated in the biological process of cold stress *** transcriptomic and RT-qPCR data showed that most of LcCBF genes displayed an initially increasing and subsequently decreasing trend during cold stress course and the expression profile of each member was *** LcCBF genes exhibited a different abundance in callus,root,stem and leaf *** structure and expression characteristics of LcCBF genes imply that they may have similar and different functions in response to cold stress *** identification and analysis of LcCBF gene family have laid the foundation for future studies into *** cold stress mechanisms and for the cultivation of cold-resistance cultivars.

关键词： Liriodendron chinense CBF genes Phylogenetic relation Expression profile Cold stress

Homojunction structure amorphous oxide thin film transistors with ultra-high mobility

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Journal of Semiconductors 2023年第5期44卷 19-26页

作者： Rongkai Lu siqin Li Jianguo Lu Bojing Lu Ruqi Yang Yangdan Lu Wenyi Shao Yi Zhao Liping Zhu Fei Zhuge Zhizhen ye State Key Laboratory of Silicon Materials Key Laboratory for Biomedical Engineering of Ministry of EducationSchool of Materials Science and EngineeringZhejiang UniversityHangzhou 310027China Wenzhou Key Laboratory of Novel Optoelectronic and Nano Materials Institute of WenzhouZhejiang UniversityWenzhou 325006China Department of Electronic Science and Technology College of Information Science and Electronic EngineeringZhejiang UniversityHangzhou 310027China Ningbo Institute of Materials Technology and Engineering Chinese Academy of SciencesNingbo 315201China

Amorphous oxide semiconductors(AOS)have unique advantages in transparent and flexible thin film transistors(TFTs)applications,compared to low-temperature polycrystalline-Si(LTPS).However,intrinsic AOS TFTs are difficult to obtain field-effect mobility(μFE)higher than LTPS(100 cm^(2)/(V·s)).Here,we design ZnAlSnO(ZATO)homojunction structure TFTs to obtainμFE=113.8 cm^(2)/(V·s).The device demonstrates optimized comprehensive electrical properties with an off-current of about1.5×10^(-11)A,a threshold voltage of–1.71 V,and a subthreshold swing of 0.372 V/*** are two kinds of gradient coupled in the homojunction active layer,which are micro-crystallization and carrier suppressor concentration gradient distribution so that the device can reduce off-current and shift the threshold voltage positively while maintaining high field-effect *** research in the homojunction active layer points to a promising direction for obtaining excellent-performance AOS TFTs.

关键词： thin film transistors homojunction carrier mobility amorphous oxides