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Enhanced immuno-detection of shed extracellular domain of HER-2/neu

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Health 2009年第4期1卷 325-329页

作者： sinang chourb Brian C. Mackness Leslie R. Farris Melisenda J. McDonald 不详

HER-2/neu oncogene is over-expressed and amplified in patients associated with metastatic breast cancer. An increased level (>15 ng/mL) in the shed extracellular domain (sECD-HER 2/neu) is indicative of the potential presence and as-sociated progression of this disease. A fluo-rescent ELISA incorporating the newly devel-oped ALYGNSA antibody-orientation system revealed a 10-fold increase in sensitivity (≤0.63 ng/mL) of sECD-HER 2/neu when compared to a control standard ELISA kit (≤7.5 ng/mL). This enhanced mode of detection has the potential to not only address breast and other cancers per se but also permit an in depth evaluation of “shed extracellular domains”, in general, and the role of these “proteolytic derived factors” in physiological signalling at normal levels.

关键词： HER-2/neu Shed Extracellular Domain C-erbB2 Breast Cancer Marker ELISA Fluorescent immunoassay ALYGNSA

Improved detection of the MUC1 cancer antigen CA 15-3 by ALYGNSA fluorimmunoassay

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Enhanced immuno-detection of shed extracellular domain of HER-2/neu

Health（健康） 2011年第8期

作者： sinang chourb Brian Christopher Mackness Leslie Ruth Farris Melisenda Jean McDonald

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Species concepts of Dothideomycetes:classification,phylogenetic inconsistencies and taxonomic standardization

Health（健康） 2009年第4期

作者： sinang chourb Brian C. Mackness Leslie R. Farris Melisenda J. McDonald

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Fungal Diversity 2021年第4期109卷 283-319页

作者： Dhandevi Pem Rajesh Jeewon Kandawatte Wedaralalage Thilini Chethana sinang Hongsanan Mingkwan Doilom Nakarin Suwannarach Kevin David Hyde Innovative Institute of Plant Health Zhongkai University of Agriculture and EngineeringHaizhu DistrictGuangzhou 510225People’s Republic of China Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand Department of Health Sciences Faculty of Medicine and Health SciencesUniversity of MauritiusReduitMauritius Faculty of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Guangdong Provincial Key Laboratory for Plant Epigenetics Shenzhen Key Laboratory of Microbial Genetic EngineeringCollege of Life Science and OceanographyShenzhen UniversityShenzhen 518060People’s Republic of China Research Center of Microbial Diversity and Sustainable Utilization Chiang Mai UniversityChiang Mai 50200Thailand

The species is one of the basic units of biological *** species concepts and recognition are essential topics in taxonomic studies and other biological *** the first part of this review,we briefly discuss the taxonomic history of the class *** the second part of the paper,we review four commonly used species concepts,focusing on morphological,ecological,biological and phylogenetic criteria and their applicability in the taxonomy of *** application and utility of the four criteria is discussed with examples in the genera Ascochyta,Cercospora and *** problems and challenges of studying Dothideomycetes are analyzed and basic guidelines for classifying species under the above criteria are provided.

关键词： Challenges DNA Fungi Morph Phylogeny Species Taxonomy

Evolution of freshwater Diaporthomycetidae(Sordariomycetes)provides evidence for five new orders and six new families

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Fungal Diversity 2021年第2期107卷 71-105页

作者： Kevin D.Hyde Dan-Feng Bao sinang Hongsanan K.W.Thilini Chethana Jing Yang Nakarin Suwannarach Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand Research Centre of Microbial Diversity and Sustainable Ultilization Chiang Mai UniversityChiang Mai 50200Thailand Innovative Institute of Plant Health Zhongkai University of Agriculture and EngineeringHaizhu DistrictGuangzhou 510225PR China Department of Entomology and Plant Pathology Faculty of AgricultureChiang Mai UniversityChiang Mai 50200Thailand College of Agriculture and Biological Sciences Dali UniversityDali 671003YunnanPR China Guangdong Provincial Key Laboratory for Plant Epigenetics Shenzhen Key Laboratory of Microbial Genetic EngineeringCollege of Life Science and OceanographyShenzhen UniversityShenzhen 518060China School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Guizhou Key Laboratory of Agricultural Biotechnology Guizhou Academy of Agricultural SciencesGuiyang 550006GuizhouChina Department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand

Cancellidium is a remarkable fungal genus which has been collected from wood submerged in freshwater and has unique conidia that are important in dispersal in running *** such a remarkable morphology,one would have expected it to be a distinct family or ***,due to the dearth of molecular evidence in related taxa,this genus has previ-ously been placed in the order Hypocreales,subclass Hypocreomycetidae of *** this study,we made three new collections of this remarkable aquatic genus from streams in China and Thailand,isolated them into culture,extracted DNA and carried out multigene phylogenetic analysis and divergence time estimation which placed the genus in *** is one of the seven subclasses of Sordariomycetes and contains 30 lineages that are only known from *** subclass is therefore of interest when considering the evolution of freshwater *** lineages of Diaporthomycetidae are morphologically unique and taxa cluster with strong support,but have weak support at the base of the *** phylogenetic and MCC trees generated in this study indicate that Aquapteridospora,Barbatosphaeriaceae,Bullimyces,Cancellidium,Ceratolenta,Conlarium,Phialemoniopsis,Pseudostanjehughesia and Rhamphoriaceae are distinct genera/families that evolved in the family/order time *** new orders Barbatosphaeriales(177 MYA),Cancellidiales(137 MYA),Ceratolentales(147 MYA),Conlariales(138 MYA)and Rhamphoriales(133 MYA)and six new families,Aquapteridosporaceae(110 MYA),Cancellidiaceae(137 MYA),Ceratolentaceae(81 MYA),Bullimycetaceae(81 MYA),Phialemoniopsaceae(59 MYA),and Pseudostanjehughesiaceae(111 MYA)are introduced with evidence from phylogenies,divergence estimates and distinct *** these families,Aquapteridosporaceae,Cancellidiaceae,Bullimycetaceae,and Pseudostanjehughesiaceae are only known from freshwater.

关键词： 14 new taxa Evolution Freshwater fungi New family New order

Towards a natural classification of Annulatascaceae-like taxa:introducing Atractosporales *** six new families

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Fungal Diversity 2017年第4期85卷 75-110页

作者： Huang Zhang Wei Dong Kevin D.Hyde Sajeewa S.N.Maharachchikumbura sinang Hongsanan D.Jayarama Bhat Abdullah M.Al-Sadi Di Zhang Yunnan Institute of Food Safety Kunming University of Science&TechnologyKunming 650500People’s Republic of China Center of Excellence for Fungal Research Mae Fah Luang UniversityChiang RaiThailand Department of Crop Sciences College of Agricultural and Marine SciencesSultan Qaboos UniversityP.O.Box 34Al-Khod 123Oman Formerly at Department of Botany Goa UniversityGoa 403206India No.128/1-J Azad Housing SocietyCurcaGoa VelhaIndia Faculty of Environmental Science&Engineering Kunming University of Science&TechnologyKunming 650500People’s Republic of China

Species with relatively small,membraneous,black ascomata,with or without long necks,unitunicate,cylindrical asci with apical rings and fusiform,hyaline ascospores with or without mucilaginous sheaths are common in freshwater habitats in tropical and temperate *** of these taxa have originally been recorded as Annulatascaceae-like *** genera have been included in the family Annulatascaceae,mostly based on morphological characters,while molecular work and phylogenetic analyses are lacking for many *** this study,nine new Annulatascaceae-like taxa collected from Thailand were morphologically *** cultures obtained from single ascospores were used in molecular *** nine new strains and several other strains of Annulatascaceae-like Sordariomycetes species were used to establish phylogenetic and evolution relationships among the taxa,based on combined LSU,SSU,ITS and RPB2 sequence *** analyses provide evidence to introduce one new order and six new families,to accommodate taxa excluded from Annulatascaceae sensu stricto.A new order Atractosporales is established based on the molecular study,including three new introduced families Conlariaceae,Pseudoproboscisporaceae and *** is introduced for the genus Conlarium which comprises two species,Conlarium duplumascosporun and a new Hyphomycetous asexual morph taxon Conlarium aquaticum which has subglobose or irregular,brown,clathrate,muriform *** includes Pseudoproboscispora and Diluviicola,while Atractosporaceae includes the genera Rubellisphaeria and ***,Xylomelasma and Ceratostomella form a distinct stable lineage which is introduced as a new family Barbatosphaeriaceae in Diaporthomycetidae families incertae sedis.A new family Lentomitellaceae is introduced in Diaporthomycetidae families incertae sedis,to accommodate the genus *** is introduced to accommodate Woswasia,Xyloch

关键词： Annulatascales Diaporthomycetidae Phylogeny Sexual morphs Submerged wood

Taxonomy and the evolutionary history of Micropeltidaceae

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Fungal Diversity 2019年第4期97卷 393-436页

作者： Xiang-Yu Zeng Hai-Xia Wu sinang Hongsanan Rajesh Jeewon Ting-Chi Wen Sajeewa S.N.Maharachchikumbura Putarak Chomnunti Kevin D.Hyde International Fungal Research and Development Centre Key Laboratory of Resource Insect Cultivation&Utilization State Forestry AdministrationThe Research Institute of Resource InsectsChinese Academy of ForestryKunming 650224China The Engineering Research Center of the Utilization for Characteristic Bio-Pharmaceutical Resources in Southwest Guizhou UniversityGuiyang 550025China Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand Shenzhen Key Laboratory of Microbial Genetic Engineering College of Life Sciences and OceanographyShenzhen UniversityShenzhen 518000China Shenzhen Key Laboratory of Laser Engineering College of Optoelectronic EngineeringShenzhen UniversityShenzhen 518000China Department of Health Sciences Faculty of ScienceUniversity of MauritiusReduit 80837Mauritius Department of Crop Sciences College of Agricultural and Marine SciencesSultan Qaboos UniverstyPO Box 8123 Al KhoudOman School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of Sciences132 Lanhei RoadKunming 650201Chin

Micropeltidaceae species are flyspeck fungi which have been subjected to few systematic *** re-examined 27 genera which were accepted in the Micropeltidaceae and re-described them based on herbaria materials and *** on morphology and phylogenetic investigations,we transfer Micropeltidaceae to a new order,Micropeltidales(Lecanoromycetes).Genera with bluish or greenish upper walls(Dictyopeltella,Dictyothyriella,Dictyothyrina,Dictyothyrium,Haplopeltheca,Micropeltis,Scolecopeltidium and Stomiopeltopsis)are accepted in the new taxonomic concept for Micropeltidaceae.A molecular clock approach estimated the divergence time of the Micropeltidaceae crown group at 130(165–104)Mya,which also supports its rank as an order(diverging from 220–100 Mya).The evolutionary histories between Micropeltidaceae species and host plants are interpreted by cophylogenetic analyses calibrated by their divergence *** result indicates that the diversification of Angiospermae(130–80 Mya)fosters the formation of genera of Micropeltidaceae mainly via cospeciation events,and this codivergent period would be an important reference when establishing generic boundaries of epifoliar fungi.

关键词： Codivergence Cophylogeny Dating Lecanoromycetes Micropeltidales

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Meliolales

Fungal Diversity 2015年第5期74卷 91-141页

作者： sinang Hongsanan Qing Tian Derek Peršoh Xiang-Yu Zeng Kevin D.Hyde Putarak Chomnunti Saranyaphat Boonmee Ali H.Bahkali Ting-Chi Wen Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China World Agroforestry Centre East and Central AsiaKunming 650201YunnanPeople’s Republic of China Institute of Excellence in Fungal Research Chiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Ruhr-Universität Bochum GeobotanyUniversitätsstraße 15044780 BochumGermany Botany and Microbiology Department College of ScienceKing Saud UniversityRiyadhKSA 11442Saudi Arabia The Engineering and Research Center of Southwest Bio-Pharmaceutical Resource Ministry of EducationGuizhou UniversityGuiyang 550025China

The order Meliolales comprises the families Armatellaceae and *** are black mildews that grow on the surface of host plants,often regarded as minor plant *** this study,types or specimens of 17 genera of Armatellaceae and Meliolaceae were borrowed from herbaria and *** is accepted in Armatellaceae and Amazonia,Appendiculella,Asteridiella,Cryptomeliola,Endomeliola,Irenopsis and Meliola are accepted in the family *** is synonymized under ***,Ectendomeliola,Haraea,Hypasteridium,Leptascospora,Metasteridium,Ophiociliomyces,Ophioirenina,Ophiomeliola,Parasteridium,Pauahia,Pleomeliola,Pleomerium,Prataprajella,Ticomyces,Urupe and Xenostigme are excluded from Meliolaceae,and are treated as doubtful genera or placed in ascomycetes genera incertae *** type species of each genus is re-described and illustrated with *** are provided and comparisons *** new species of Meliola and one new species of Irenopsis are also introduced with molecular data and we provide the most populated phylogenetic tree of Meliolomycetidae to *** thailandicum was found on Dimocarpus longan(Sapindales)and Acacia auriculiformis(Fabales)and confirmed to be the same species in the molecular *** has important implications as the several hundred Meliola species are recognized based on host *** the same species being recorded from two unrelated hosts sheds doubt on Meliola species being host-specific.

关键词： Armatella Armatellaceae Ascomycota Black mildews Meliola Meliolaceae Meliolomycetidae Sordariomycetes Morphology Type Species Taxonomy

An updated phylogeny of Sordariomycetes based on phylogenetic and molecular clock evidence

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Fungal Diversity 2017年第3期84卷 25-41页

作者： sinang Hongsanan Sajeewa S.N.Maharachchikumbura Kevin D.Hyde Milan C.Samarakoon Rajesh Jeewon Qi Zhao Abdullah M.Al-Sadi Ali H.Bahkali Key Laboratory of Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanPeople’s Republic of China Center of Excellence in Fingal Research Mae Fah Luang UniversityChiang Rai 57100Thailand Department of Crop Sciences College of Agricultural and Marine SciencesSultan Qaboos UniversityP.O.Box 34Al-Khod 123Oman Department of Health Sciences Faculty of ScienceUniversity of MauritiusReduitMauritius Department of Botany and Microbiology College of ScienceKing Saud UniversityP.O.Box:2455Riyadh 1145Saudi Arabia

The previous phylogenies of Sordariomycetes by ***,*** and ***,and *** and ***,were mainly based on morphology and thus were somewhat *** outlines by *** and ***,and Maharachchikumbura and co-authors,took into account phylogenetic ***,even these phylogenetic driven arrangements for Sordariomycetes,were somewhat subjective,as the arrangements in trees depended on many variables,such as number of taxa,different gene regions and methods used in the *** is needed is extra evidence to help standardize ranking in the *** of divergence times using molecular clock methods has been proposed for providing additional rational for higher ranking of ***,in Sordariomycetes,a divergence period(i.e.200–300 MYA)can be used as criteria to judge when a group of related taxa evolved and what rank they should be *** this paper,we provide an updated classification of accepted subclasses,orders of Sordariomycetes and use divergence times to provide additional evidence to stabilize ranking of taxa in the *** point out and discuss discrepancies where the phylogenetic tree conflicts with the molecular clock.

关键词： Class Classification Divergence times Phylogenetics Ranking