检索结果-南通市图书馆

Antimicrobial Activity of the Methanolic Fraction of Bamboo Pyroligneous Liquor

Journal of Pharmacy and Pharmacology 2018年第10期6卷 924-934页

作者： Luciana Jankowsky simone possedente de lira Francisco André Ossamu Tanaka Ivaldo Pontes Jankowsky José Otávio Brito department of Forest Sciences College of Agriculture"Luiz de Queiroz"University of Sao Paulo(ESALQ/USP)PiracicabaP.O.Box 09Brazil department of Exact Sciences College of Agriculture"Luiz de Queiroz"University of Sao Paulo(ESALQ/USP)Piracicaba P.O.Box 09Brazil department Phytopathology and Nematology College of Agriculture"Luiz de Queiroz"University of Sao Paulo(ESALQ/USP)Piracicaba P.O.Box 09Brazil Forestry Science and Research Institute(IPEF) Piracicaba P.O.Box 13415-000Brazil

Considering that the pyroligneous liquor obtained from pyrolysis of various types of biomass has antiviral activity, the main objective of the present study was to evaluate the antimicrobial action of the methanolic fraction extracted from bamboo （dendrocalamus asper） pyroligneous liquor in relation to Escherichia coli and Staphylococcus aureus bacteria. The pyroligneous liquor was recovered by laboratorial bamboo pyrolysis at 350 °C and then fractioned by liquid-liquid separation with methanol and hexane. After solvent evaporation, we collected the methanolic extract, which was later diluted in methanol and used in the diffusion disc assay of E. coli and S. aureus at concentrations of 0.125, 0.500, 0.750 and 1.000 mg. Through GC/MS analysis, it was possible to qualitatively identify 92 chemical compounds in the methanolic fraction. Treatment with the methanolic fraction inhibited cellular growth and caused a variety of morphological variations in size as well as deformities in the bacterial cell walls, establishing an antimicrobial activity profile. This finding supports the National Policy for Incentives for Sustainable Management and Cultivation of Bamboo （PNMCB） and has benefits to the society and environment as a whole, as it demonstrates the possibility of adding value to a byproduct of pyrolysis.

关键词： dendrocalamus asper Escherichia coli Staphylococcus aureus.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular and Chemical Analyses of Cyanobacterial Blooms in Tropical Lagoons from Southeast Brazil

引用

Journal of Water Resource and Protection 2015年第1期7卷 50-71页

作者： Luciana Mecatti Elias Maria Estela Silva-Stenico Danillo Oliveira Alvarenga Janaina Rigonato Marli Fátima Fiore simone possedente de lira department of Exact Sciences Escola Superior de Agricultura “Luiz de Queiroz” Universidade de Sao Paulo Piracicaba Brazil Centro de Energia Nuclear na Agricultura Universidade de Sao Paulo Piracicaba Brazil

The genetic diversity and the potential toxicity of bloom-forming cyanobacteria were studied in four lagoons located in the state of Sao Paulo (Campinas, Limeira and Piracicaba cities). Bloom samples were collected on the water surface and cyanobacterial communities were evaluated using DGGE fingerprinting and 16S rDNA clone library. The amplification of genes encoding secondary metabolites such as microcystin (mcy), anatoxin (ana), cylindrospermopsin (cyr), saxitoxin (sxt), cyanopeptolin (mcn) and aeruginosin (aer) was performed and their production analyzed by LC-MS. The comparison of DGGE banding pattern among the different water samples suggested that some operational taxonomic units (OTUs) in these locations were predominant over others. The 16S rDNA clone libraries sequences matched with nine different known cyanobacterial genera available in NCBI, identified as Anabaena, Brasilonema, Cylindrospermopsis, Limnococcus, Microcystis, Nostoc, Pseudanabaena, Synechococcus and Woronichinia. The lagoons ESALQ2, Taquaral and Limeira had more than 80% of the cyanobacterial community assigned to the genus Microcystis. Genes encoding aeruginosin, cyanopeptolin and microcystin synthetases and saxitoxin synthase were amplified, and LC-MS/MS confirmed the production of aeruginosin, cyanopeptolin and microcystin. Rapid and sensitive methods for the detection of these secondary metabolites, especially toxins, using chemical and molecular tools together, can be used for a faster diagnostic of toxic cyanobacterial blooms.

关键词： Cyanobacteria Bloom Cyanotoxins Lagoons Microcystis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Alpha-Tomatin against Witches’Broom Disease

引用

American Journal of Plant Sciences 2014年第5期5卷 596-604页

作者： Luiz Humberto Gomes Keila Maria Roncato Duarte Felipe Gabriel Andrino Gildemberg Amorin Leal Jr Lia Matelli Garcia Antonio Figueira simone possedente de lira Laboratory of Natural Products Environmental ChemistryDepartment of Exact SciencesEscola Superior de Agricultura“Luiz de Queiroz”Universidade de Sao PauloPiracicabaBrazil Institute of Animal Science and Pastures Nova OdessaBrazil Federal University of Alagoas MaceioBrazil Centro de Energia Nuclear na Agricultura Universidade de Sao PauloPiracicabaBrazil

Several plants use secondary metabolites against insects and pathogens attack. Among such metabolites known glycoalkaloids are the most studied. Plants from Solanaceae Family are the most abundant on those alkaloids. despite alfa-tomatine is a known glycoalkaloid, its specific action against Moniliophthora perniciosa, fungi responsible for witches broom disease in cocoa plantations was obtained in this work. Alfa-tomatine was infusion-extracted from Solanum lycopersicum leaves, and its action against the fungi growth was shown on the bioassays. In these tests, M. perniciosa growth was totally inhibited but other fungus tested, the inhibition was not totally, or did not present effect on growth, showing in this way, the specificity of alfa-tomatine to M. perniciosa. The advantages of work with a natural molecule for the fungi control is the stability of the molecule, its resistance to high temperature and pressure and for its use, the simple method of extraction used. The confirmation of the alfa-tomatine molecule was done by High Performance Liquid Chromatography and Mass Spectrometry in comparison to the Sigma standard.

关键词： Cocoa Glycoalkaloids Solanaceae

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pressure Ulcers in Institutionalized Elderly People:Association of Sociodemographic and Clinical Characteristics and Risk Factors

引用

Open Journal of Nursing 2017年第1期7卷 111-122页

作者： Suellen Duarte de Oliveira Matos Iraktania Vitorino Diniz Adriana lira Rufino de Lucena Smalyanna Sgren da Costa Andrade Karen Krystine Goncalves de Brito Elizabeth Souza Silva de Aguiar Emanuelle Malzac Freire de Santana Mailson Marques de Sousa Ana Paula Marques Andrade de Souza Hemílio Fernandes Campos Coelho Mirian Alves da Silva Marta Miriam Lopes Costa Maria Julia Guimaraes Oliveira Soares simone Helena dos Santos Oliveira Graduate Program in Nursing Universidade Federal da ParaíbaJoao PessoaBrazil departament of Statistics Universidade Federal da ParaíbaParaíbaBrazil

Objective: To evaluate the association of socio-demographic and clinical characteristics, and risk factors with occurrence of pressure ulcers in institutionalized elderly people. Method: This cross-sectional, analytical study, in quantitative approach, performed from the database analysis linked to the project “Pressure Ulcer in institutionalized elderly people: Association of incidence with the risk factors to functional and nutritional assessment” developed in six long-stay institutions for the elderly people in João Pessoa. Data were analyzed using the Statistical Package for Social Sciences (SPSS) version 20.0. Associations were made through chi square test and Odds Ratio. Results: The clinical conditions of significant associations with the occurrence of pressure ulcers were the variables neurological disorders (p = 0.011) and visual impairment (p = 0.005). As for risk factors, the most important was fecal incontinence (p Conclusion: The analysis of the associations pointed out problems that require intervention in order to prevent health implications of the elderly people and to minimize the risk and occurrence of this injury.

关键词： Elderly People Institutions for the Elderly People Pressure Ulcer Health Profile Risk Factors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：