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Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli

Asian pacific Journal of Tropical Medicine 2022年第2期15卷 71-78页

作者： Guilherme S.Machado Rosane L.Lopes simone S.c.Oliveira simone p.c.freitas Jacenir R.Santos-Mallet AndréL.S.Santos Denise Feder Suzete A.O.Gomes programa de pós-Graduação em ciências e Biotecnologia Instituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil Laboratório de Biodiversidade de Insetos e patógenos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes Departamento de Microbiologia GeralInstituto de Microbiologia Paulo de GóesUniversidade Federal do Rio de JaneiroRJBrazil Fundação Oswaldo cruz piauí TeresinaPIBrazil Laboratório Interdisciplinar de Vigilância Entomológica em Díptera e Hemíptera Instituto Oswaldo CruzFundação Oswaldo CruzRio de JaneiroRJBrazil Universidade Iguaçu Nova IguaçuRJBrazil Laboratório de Biologia de Insetos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil

Objective:To investigate the morphological structure of ovarian follicular cells and biochemical parameters of both ovaries and fat bodies(sites of vitellogenesis)from Rhodnius(R.)prolixus infected with Trypanosoma(T.)***:Adult virgin females of *** were fed upon a membrane apparatus containing heat-inactivated citrated rabbit blood and a suspension of *** epimastigotes(Macias strain).Females from the control group and all the males received parasite-free *** electron microscopy was used to reveal the morphological aspects of ovarian follicle cells in both control and parasite-infected *** profile,proteolytic activities and Western blotting analyses were performed in either ovary or fat body samples of control and parasite-infected ***:According to the ultrastructural data,*** infection elicited a degeneration process in the ovarian follicular cells of *** assays indicated a reduction in the activity of aspartic peptidases in the ovary and fat body from parasite-infected group,while a significant increase in the cysteine peptidase activity was measured in both insect ***,immunoblotting revealed that vitellogenin was overexpressed in the ovary of parasite-infected ***:*** infection seems to elicit an early programmed cell death in the ovarian follicle cells as well as induces the modulation on the activities of different peptidase classes in either ovaries or fat bodies and the overexpression of the vitellogenin in the ovary of ***.

关键词： Trypanosoma rangeli Rhodnius prolixus Ovarian follicle Vitellogenin Aspartic protease cysteine protease

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The initial stages of Li_(2)O_(2) formation during oxygen reduction reaction in Li-O_(2) batteries:The significance of Li_(2)O_(2) in charge-transfer reactions within devices

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Journal of Energy chemistry 2024年第1期88卷 223-231,I0006页

作者： Daniela M.Josepetti Bianca p.Sousa simone A.J.Rodrigues Renato G.freitas Gustavo Doubek Advanced Energy Storage Division LABCenter for Innovation on New EnergiesSchool of Chemical EngineeringUniversity of CampinasAv Albert Einstein 50013083-852CampinasSPBrazil Institute of physics&Department of chemistry Laboratory of Computational MaterialsFederal University of Mato Grosso78060-900CuiabáMTBrazil

Lithium-oxygen batteries are a promising technology because they can greatly surpass the energy density of lithium-ion ***,this theoretical characteristic has not yet been converted into a real device with high *** with air contamination,metallic lithium reactivity,and complex discharge and charge reactions are the main issues for this technology.A fast and reversible oxygen reduction reaction(ORR)is crucial for good performance of secondary batteries',but the partial knowledge of its mechanisms,especially when devices are concerned,hinders further *** this perspective,the present work uses operando Raman experiments and electrochemical impedance spectroscopy(EIS)to assess the first stages of the discharge processes in porous carbon electrodes,following their changes cycle by cycle at initial operation.A growth kinetic formation of the discharge product signal(Li_(2)O_(2))was observed with operando Raman,indicating a first-order reaction and enabling an analysis by a microkinetic *** solution mechanism in the evaluated system was ascribed for an equivalent circuit with three time *** the time constant for the anode interface reveals to remain relatively constant after the first discharge,its surface seemed to be more *** model indicated that the reaction occurs at the Li_(2)O_(2) surface,decreasing the associated resistance during the initial discharge ***,the growth of Li_(2)O_(2) forms a hetero-phase between Li_(2)O_(2)/electrolyte,while creating a more compact and homogeneous on the Li_(2)O_(2)/cathode *** methodology here described thus offers a way of directly probing changes in surface chemistry evolution during cycling from a device through EIS analysis.

关键词： Li-O_(2)battery Operando Raman analysis Equivalent circuit modeling Time-constant distribution

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Oxidative reforming of methane for hydrogen and synthesis gas production:Thermodynamic equilibrium analysis

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Journal of Natural Gas chemistry 2012年第5期21卷 571-580页

作者： Antonio c.D.freitas Reginaldo Guirardello School of chemical Engineering University of Campinas (UNICAMP)Av.Albert Einstein 50013083-852Campinas-SPBrazil

A thermodynamic analysis of methane oxidative reforming was carried out by Gibbs energy minimization （at constant pressure and temperature） and entropy maximization （at constant pressure and enthalpy） methods,to determine the equilibrium compositions and equilibrium temperatures,*** cases were treated as optimization problems （non-linear programming formulation）.The GAMS 23.1 software and the cONOpT2 solver were used in the resolution of the proposed *** hydrogen and syngas production were favored at high temperatures and low pressures,and thus the oxygen to methane molar ratio （O 2 /cH 4） was the dominant factor to control the composition of the product *** O 2 /cH 4 molar ratios higher than 0.5,the oxidative reforming of methane presented autothermal behavior in the case of either utilizing O 2 or air as oxidant agent,but oxidation reaction with air possessed the advantage of avoiding peak temperatures in the system,due to change in the heat capacity of the system caused by the addition of *** calculated results were compared with previously published experimental and simulated data with a good agreement between them.

关键词： thermodynamic analysis methane oxidative reforming Gibbs energy minimization entropy maximization hydrogen and syngas production

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Rice spouted bed for manufacturing inclusion complexes of Endopleura uchi L extracts in β-cyclodextrin

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particuology 2019年第1期17卷 208-215页

作者： L.V.D. freitas A.c.R. Montes E.I.A. campos E.c. conceicao L.A. Tacon A.D. Lanchote L.A.p.freitas Faculdade de ciencias Farmaceuticas de Ribeirao preto University of Sao PauloVia do Cafe s/n14040-903Ribeirao PretoSPBrazil

Uxi (Endopleura uchi) is a plant from the Brazilian Amazon. It has many biological activities that are attributed to its main active component, bergenin (BG), which has low water solubility. The aim of this work was to obtain β-cyclodextrin (β-cD) inclusion complexes of uxi using lyophilized cooked rice as the solid substrate. The process was studied using a Box–Behnken design with the solution feed rate, molar ratio of β-cD to BG, and rice bed load as factors. These factors greatly affected the product bulk density, angle of repose, water content, and BG content, loss, and water solubility. The optimum process conditions determined using a desirability functions methodology were an extract feed rate of 1 mL/min, a ratio of β-cD to BG of 3:1, and a substrate load of 100 g. The predicted values under the optimum conditions were close to the experimental results of a water content of 5.8%± 0.2%, BG content of 985.7 ± 15.4 μg/g, BG water solubility of 2230.2 ± 21.1 μg/mL, and BG loss of 1.2%± 0.1%. These results confirm that spouted beds are an important tool for rapid, one-step preparation of pharmaceutical β-cD inclusion complexes in a solid form.

关键词： Bergenin chromatography Yield Active loss Solubility

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Magnetic Tunnel Junction Based on MgO Barrier prepared by Natural Oxidation and Direct Sputtering Deposition

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Nano-Micro Letters 2012年第1期4卷 25-29页

作者： Xiaohong chen paulo.p.freitas Engineering Research center for Nanophotonics and Advanced Instrument Ministry of Education Department of Physics East China Normal University INESc-MN

Magnetic tunnel junctions(MTJs) based on MgO barrier have been fabricated by sputtering single crystal MgO target and metal Mg target, respectively, using magnetic sputtering system Nordiko 2000. MgO barriers have been formed by a multi-step deposition and natural oxidization of Mg layer. Mg layer thickness,oxygen flow rate and oxidization time were adjusted and the tunnel magnetoresistance(TMR) ratio of optimal MTJs is over 60% at annealing temperature 385. The(001) MgO crystal structure was obtained when the separation distance between MgO target and substrate is less than 6 cm. The TMR ratio of most MgO based MTJs are over 100% at the separation distance of 5 cm and annealing temperature 340. The TMR ratios of MTJs are almost zero when the separation distance ranges from 6 to 10 cm, due to the amorphous nature of the MgO film.

关键词： Magnetic tunnel junctions Mg O crystal structure Magnetic sputtering system

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Inclusive housing program： The case of Oe-cusse region in East Timor

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Frontiers of Architectural Research 2017年第1期6卷 74-88页

作者： Miguel p. Amadoa Ines Ramalhete Antonio Ribeiro Amado Joao c. freitas cERIS - civil Engineering Research and Innovation for Sustainability IST Universidade de Lisboa Lisboa 1049-001 Portugal GEOTpU LAB Instituto Superior Tecnico Universidude de Lisboo Lisboo 1049-001 Portugul ccIAUD Faculty of Architecture Universidude de Lisboo Lisboo 1049-001 Portugal

Housing development has become one of the main strategies to alleviate impoverished communities in developing countries. In the past, policies and programs focused on either economic or social aspects, and numerous imported solutions had failed to produce satisfactory results. At present, holistic approaches integrating all dimensions of human activity and environmental impact are gaining recognition as suitable alternatives for various experiences in developing nations. However, these approaches do not properly consider the local context, which is different for each case, thereby leading to inadequate models and unsuccessful implementations. This paper presents a context-driven and integrated approach for rural areas in developing nations for one of the most basic human endeavors, i.e., constructing a house. The self-building process utilized in constructing houses is a major strategy to help communities overcome poverty. Such a house must be capable of accommodating economic activities; must be durable yet flexible in layout and size; and must offer all basic services, including water, sanitation, and energy. consequently, the Inclusive Housing program is developed and employed in Oe-cusse, a remote region of East Timor, as an integral part of the Regional plan.

关键词： Developing countries Rural settlements Housing program Adaptive housing

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Lack of correlation between p53 codon 72 polymorphism and anal cancer risk

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World Journal of Gastroenterology 2009年第36期15卷 4566-4570页

作者： simone S contu Grasiela Agnes Andrea p Damin paulo c contu Mário A Rosito claudio O Alexandre Daniel c Damin Department of Surgery Division of Coloproctology Clinicas Hospital of Porto Alegre and Federal University of Rio Grande do Sul90.035-930 Porto AlegreBrazil Laboratory of Molecular Biology and Department of pathology Federal University of Health Science of Porto Alegre90.035-930 Porto AlegreBrazil

AIM:To investigate the potential role of p53 codon 72 polymorphism as a risk factor for development of anal cancer. METHODS:Thirty-two patients with invasive anal carcinoma and 103 healthy blood donors were included in the study.p53 codon 72 polymorphism was analyzed in blood samples through polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing. RESULTS:The relative frequency of each allele was 0.60 for Arg and 0.40 for pro in patients with anal cancer, and 0.61 for Arg and 0.39 for pro in normal controls. No significant differences in distribution of the codon 72 genotypes between patients and controls were found. cONcLUSION:These results do not support a role for the p53 codon 72 polymorphism in anal carcinogenesis.

关键词： Anus neoplasms Arginine Geneticpolymorphism polymerase chain reaction proline Tumor suppressor protein p53

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二维导流管喷动床的流动特征

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化工冶金 2000年第4期21卷 369-374页

作者：罗保林 freitas L A p Lim c J Grace J R 中国科学院化工冶金研究所北京100080 加拿大不列颠哥伦比亚大学化工系

带导流管的二维喷动床(2-DSBDp)是传统喷动床的改进型式，矩形床内设置的与床同厚的垂直导流管可以控制固体颗粒的循环速率, 同时使下行区中的气固移动床维持平推流. 本文实验测定了不同表观气速、床层高度、固体颗粒与气体入口尺寸时,... 详细信息

带导流管的二维喷动床(2-DSBDp)是传统喷动床的改进型式，矩形床内设置的与床同厚的垂直导流管可以控制固体颗粒的循环速率, 同时使下行区中的气固移动床维持平推流. 本文实验测定了不同表观气速、床层高度、固体颗粒与气体入口尺寸时, 二维导流管喷动床的床压降及相应的空隙率，藉以阐述2-DSBDp的流动特征.

关键词：二维喷动床导流管流动特征

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Get it white: color-tunable Ac/Dc OLEDs

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Light(Science & Applications) 2015年第1期4卷 492-497页

作者： Markus Frobel Tobias Schwab Mona Kliem simone Hofmann Karl Leo Malte c Gather Institute for Applied photophysics(IApp) Dresden University of TechnologyD-01069 DresdenGermany School of physics and Astronomy University of St AndrewsSt Andrews KY169SSUK

Organic light-emitting diodes(OLEDs)have gained considerable attention because of their use of inherently flexible materials and their compatibility with facile roll-to-roll and printing *** addition to high efficiency,flexibility and transparency,reliable color tunability of solid state light sources is a desirable feature in the lighting and display ***,we demonstrate a device concept for highly efficient organic light-emitting devices whose emission color can be easily adjusted from deep-blue through cold-white and warm-white to saturated *** approach exploits the different polarities of the positive and negative half-cycles of an alternating current(Ac)driving signal to independently address a fluorescent blue emission unit and a phosphorescent yellow emission unit which are vertically stacked on top of each *** electrode design is optimized for simple fabrication and driving and allows for two-terminal operation by a single *** presented concept for color-tunable OLEDs is compatible with application requirements and versatile in terms of emitter combinations.

关键词： alternating current color mixing color tuning vertical stacking white organic light-emitting devices

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Experimental and numerical analysis on suitability of S-Glass-carbon fiber reinforced polymer composites for submarine hull

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Defence Technology（防务技术） 2023年第1期19卷 1-11页

作者： Elango Natarajan Lídio Inacio freitas M.S.Santhosh Kalaimani Markandan Ammar Abdulaziz Majeed Al-Talib c.S.Hassan Faculty of Engineering Technology and Built EnvironmentUCSI UniversityKuala LumpurMalaysia Dili Institute of Technology DiliTimor-Leste Selvam composite Materials Research Laboratory Department of Mechanical EngineeringSelvam College of TechnologyTamil NaduIndia

Suitability of S-Glass/carbon fiber reinforced polymer composite for submarine hull subjected to hydrostatic pressure has been investigated in the present *** materials have raised concerns owing to their decomposition due to low resistance towards salinity and hence polymer composites have been explored to showcase their mechanical stability to withstand transverse and impact *** this end,the mechanical properties of S-Glass/carbon fiber reinforced polymer composite were experimentally investigated and higher specific strength and stiffness of the composite in comparison to many metallic materials used for submarine hull were *** obtained experimental values were used for the static and dynamic crash analysis of the bow,stern and foil through Finite Element Analysis(FEA);where depth of travel was varied from sea surface level of 0-7000 *** assembly was later developed with the optimum shape and thickness of each *** also report the nonlinear crash analysis upon impact at velocity ranging from 3 to 21 m/***,kinetic energy,acceleration peak and internal energy in struck submarine revealed that travel depth 1750 m and 3500 m is recommendable,more particularly,crash safety factor of the submarine is found to be within limit when submarine encounters crash at 1750 m.

关键词： crash Impact Design Submarine Hull Bow Stern Foil

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