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Journal of Advanced Ceramics 2021年第6期10卷 1350-1359页

作者： Fan ZHANG Hailong hu simin hu Jianling YUE School of Minerals Processing and Bioengineering Central South University School of Aeronautics & Astronautics Central South University Research Center in Intelligent Thermal Structures for Aerospace Central South University

Carbon fibre（CF） embedded into elastomeric media has been attracting incredible interest as flexible strain sensors in the application of skin electronics owing to their high sensitivity in a very small strain gauge. To further improve the sensitivity of CF/PDMS composite strain sensor, the relatively low temperature prepared TiO2nanowire via hydrothermal route was employed herein to functionalize *** results showed a significant increase in the sensitivity of the TiO2@CF/PDMS composite strain sensors which was reflected by the calculated gauge factor. As the prepared TiO2nanowire vertically embraced the surroundings of the CF, the introduced TiO2nanowire contributed to a highly porous structure which played a predominant role in improving the sensitivity of strain sensors. Moreover,the significant strain rate dependent behavior of TiO2@CF/PDMS strain sensor was revealed when performing monotonic tests at varied strain rate. Therefore, introducing TiO2nanowire on CF offers a new technique for fabricating flexible strain sensors with improved sensitivity for the application of flexible electronics.

关键词： composites dependence of strain rate sensitivity flexible strain sensors

悬浮物对三亚珊瑚礁区浮游动物群落结构及其摄食的影响

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热带海洋学报 2024年第3期43卷 122-130页

作者：胡思敏周天成张琛刘胜李涛黄晖中国科学院热带海洋生物资源与生态重点实验室广东省应用海洋生物学重点实验室中国科学院南海海洋研究所广东广州510301 三亚海洋科学综合(联合)实验室海南省热带海洋生物技术重点实验室三亚海洋生态环境工程研究院海南三亚572000 中国科学院大学北京100049 海南三亚海洋生态系统国家野外科学观测研究站中国科学院海南热带海洋生物实验站海南三亚572000

随着人类活动对珊瑚礁的影响日益加剧,输入到近岸珊瑚礁区的悬浮物日益增多,不仅影响珊瑚的生理,也会对珊瑚礁生态系统基础生物生产过程产生影响。为了解悬浮物对珊瑚礁区浮游动物组成及摄食的影响,于2015年4月在三亚典型岸礁区设置了... 详细信息

随着人类活动对珊瑚礁的影响日益加剧,输入到近岸珊瑚礁区的悬浮物日益增多,不仅影响珊瑚的生理,也会对珊瑚礁生态系统基础生物生产过程产生影响。为了解悬浮物对珊瑚礁区浮游动物组成及摄食的影响,于2015年4月在三亚典型岸礁区设置了昼夜连续观测站点(W4为悬浮物浓度高的站位,W5为对照站位),分析了悬浮物波动对浮游动物群落结构的影响,并用分子生物学方法结合室内实验,研究了悬浮物对优势桡足类现场食物组成和摄食选择的影响。结果显示:两个站位叶绿素a浓度偏低,昼夜波动较大;W4站的悬浮物和叶绿素a浓度均高于W5站,但W5站悬浮物中有机颗粒物组分比例(73.69%)高于W4站(46.09%);W5站浮游动物种类多样性和丰度均高于W4站。进一步摄食分析表明,两个站位桡足类均以后生动物来源的有机碎屑为主要食物,在W4站桡足类食物组成中浮游植物的比例也相对较高,而在W5站桡足类还摄食较多的微型浮游动物。在摄食实验中,高浓度的悬浮颗粒物显著提高了红纺锤水蚤(Acartia erythraea)的滤水率和摄食率,但其食物组成却由以浮游植物为主转而以陆生植物碎屑为主,说明浓缩海水中较高的悬浮颗粒物含量限制了桡足类的摄食选择,使其摄食大部分有机碎屑作为补偿营养来源。研究结果表明,高悬浮物浓度会影响浮游动物的摄食选择,但悬浮物中高比例的有机碎屑可为浮游动物提供重要的补充食物来源,支持其维持较高的生物量。

关键词：浮游动物悬浮物群落结构摄食多样性三亚湾

南沙群岛海域珊瑚礁区三种寄居蟹的摄食差异比较

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热带海洋学报 2024年第3期43卷 109-121页

作者：贾男周天成胡思敏张琛黄晖刘胜中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室广东省应用海洋生物学重点实验室广东广州510301 中国科学院大学北京100049 三亚海洋科学综合(联合)实验室海南省热带海洋生物技术重点实验室三亚中科海洋研究院海南三亚572000 海南三亚海洋生态系统国家野外科学观测研究站中国科学院海南热带海洋生物实验站海南三亚572000

寄居蟹是海洋底栖动物,珊瑚礁区的复杂结构使其成为寄居蟹多样性最高的区域之一。在底栖藻类覆盖率上升的环境变化下,寄居蟹等小型底栖生物可以快速适应食物资源变动,与植食性鱼类发挥相似的功能,在维护珊瑚礁生态系统健康中的作用不容... 详细信息

寄居蟹是海洋底栖动物,珊瑚礁区的复杂结构使其成为寄居蟹多样性最高的区域之一。在底栖藻类覆盖率上升的环境变化下,寄居蟹等小型底栖生物可以快速适应食物资源变动,与植食性鱼类发挥相似的功能,在维护珊瑚礁生态系统健康中的作用不容忽视,但目前对不同寄居蟹现场食物组成差异和生态功能定位知之甚少。本研究于2019年春季在南沙群岛海域珊瑚礁区采集三种优势寄居蟹:兔足真寄居蟹(Dardanus lagopodes)、莫氏硬壳寄居蟹(Calcinus morgani)和沟纹纤毛寄居蟹(Ciliopagurus strigatus),运用高通量测序技术分析其现场食物组成。研究结果显示,三种寄居蟹的食物种类有9个门类,包括红藻门(Rhodophyta)、甲藻门(Pyrrophyta)、褐藻门(Phaeophyta)、绿藻门(Chlorophyta)、节肢动物门(Arthropoda)、苔藓动物门(Bryozoa)等。三种寄居蟹均以藻类为主要食物,包含大量的红藻和甲藻(37.75%和21.14%),此外还有少量的绿藻(3.13%)。不同寄居蟹种类的食物组成有特异性:莫式硬壳寄居蟹主要以草皮海藻和碎屑为食,是植食性寄居蟹;沟纹纤毛寄居蟹以礁石表生藻席(epilithic algal matrix,EAM)中的介形类为主要食物来源,伴随一些枝状珊瑚藻和丝状褐藻;而兔足真寄居蟹选择摄食壳状珊瑚藻(crustose coralline algae,CCA)、大型藻类及附生其上的苔藓虫。结合生态位重叠度分析发现,三种寄居蟹的重叠度很低(0.059)。在以CCA为优势底栖藻类的退化珊瑚礁区,兔足真寄居蟹与另外两种寄居蟹的摄食区域可能并不重叠,主要在清除CCA和入侵性苔藓虫,维护珊瑚礁健康发展中发挥重要作用。而沟纹纤毛寄居蟹和莫氏硬壳寄居蟹作为次级消费者,将能量更高效地向上层传递。本研究结果拓展了以往对寄居蟹生态地位的认识。

关键词：寄居蟹食性高通量测序生态功能珊瑚礁

Dietary separation between co-occurring copepods in a food-limited tropical coral reef of the Sanya Bay

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Acta Oceanologica Sinica 2020年第4期39卷 65-72页

作者： simin hu Tao Li Sheng Liu hui huang Key Laboratory of Tropical Marine Bio-Resources and Ecology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Guangdong Provincial Key Laboratory of Applied Marine Biology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Tropical Marine Biological Research Station in Hainan Chinese Academy of SciencesSanya 572000China

Food differentiation among coexistent species in the field is important strategy for copepods to acquire materials and maintain population *** situ diet analysis of co-occurring six copepod species in coral waters of the Sanya Bay was conducted using a PCR protocol based on 18 S ribosomal *** prey organisms were uncovered,including dinoflagellate,diatom,green algae and plant,protozoa and *** these spatially coexisting six species showed different dietary diversity,with the food niche breadth(B)ranging from 1.00(Temora turbinate in morning)to 10.68(Calanopia elliptica in night).While food overlap between all these copepods were low,with the average value of the diet niche overlap index being approximately *** temporally co-existing species sampled from the same time point fed on different groups of prey items with the food overlap index of 0.04 to 0.07 in midday and night but 0 in *** the most important dominant copepod in the Sanya Bay,Subeucalanus subcrassus seems to be capable to regulate its feeding,by exhibiting a rhythm of herbivorous feeding in midday and carnivorous feeding in morning and night,to better coordinate with other competitors for utilization of food *** most copepods,none of the prey items belonged to the dominant phytoplankton in the ambient water,indicating that copepod can better their survival by widening the choice of potential food resources in food limited *** dietary separation observed here might be important strategy for copepod to maintain population stabilization and thriving in the Sanya coastal waters.

关键词： copepod food partitioning Sanya Bay coral reef ecosystem

Genome-wide identification and characterization of the lateral organ boundaries domain gene family in Brassica rapa ***

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Plant Diversity 2020年第1期42卷 52-60页

作者： Qin Yu simin hu Jiancan Du Yongping Yang Xudong Sun Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of Botany.Chinese Academy of ScienceKunming 650201China Plant Germplasm and Genomics Center The Cermplasm Bank of Wild SpeciesKunming Institute of BotanyChinese Academy of SciencesKunming 650201 China Institute of Tibetan Plateau Research at Kunming.Kunming Institute of Botany Chinese Academy of Sciences.Kunming 650201China University of the Chinese Academy of Sciences Beijing 100049China

The Lateral Organ Boundaries Domain(LBD)genes encode highly conserved plant-specific LOB domain proteins which regulate growth and development in various ***,members of the LBD gene family have yet to be identified in Brassica rapa *** the present study,fifty-nine LBD genes were identified and distributed on 10 *** BrrLBD proteins are predicted to encode hydrophobic polypeptides between 118 and 394 amino acids in length and with molecular weights ranging from 13.31 to 44.24 kDa;the theoretical pi for these proteins varies from 4.83 to *** were 17 paralogous gene pairs in the BrrLBD family,suggesting that the amplification of the BrrLBD gene family involved largescale gene duplication *** of the BrrLBD family were divided into 7 subclades(class I a to e,class II a and b).Analysis of gene structure and conserved domains revealed that most BrrLBD genes of the same subclade had similar gene structures and protein *** expression profiles of 59 BrrLBD genes were determined through Quantitative Real-time fluorescent PCR(qRT-PCR).Most BrrLBD genes in the same subclade had similar gene expression ***,the expression patterns of 7 genes differed from their duplicates,indicating that although the gene function of most BrrLBD genes has been conserved,some BrrLBD genes may have undergone evolutionary change.

关键词： LBD Transcription factors LBD gene sequence analysis Expression profiles Brassica rapa var.rapa

Molecular quantification of copepod Acartia erythraea feeding on different algae preys

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Acta Oceanologica Sinica 2023年第9期42卷 125-131页

作者： simin hu Tao Li hui huang Sheng Liu Key Laboratory of Tropical Marine Bio-resources and Ecology Chinese Academy of SciencesGuangzhou 510301China Guangdong Provincial Key Laboratory of Applied Marine Biology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China CAS-HKUST Sanya Joint Laboratory of Marine Science Research/Key Laboratory of Tropical Marine Biotechnology of Hainan Province Sanya Institute of Ocean Eco-Environmental EngineeringSouth China Sea Institute of OceanologySanya 572000China Sanya National Marine Ecosystem Research Station Tropical Marine Biological Research Station in HainanChinese Academy of SciencesSanya 572000China

Quantitative evaluation of the copepod feeding process is critical for understanding the functioning of marine food webs, as this provides a major link between primary producers and higher trophic levels. In this study, a molecular protocol based on quantitative polymerase chain reaction(qPCR) targeting 18S rDNA was developed and used to investigate the feeding and digestion rates of the copepod Acartia erythraea in a laboratory experiment using microalgae Thalassiosira weissflogii, Prorocentrum shikokuense, and Alexandrium catenella as prey. Although offered an equal encounter rate based on biovolume, prey uptake varied substantially among the three algal species, with the ingestion rate(IR) and digestion rate(DR) of A. erythraea differing significantly(P <0.001) based on both cell counting and qPCR detection. Acartia erythraea showed the highest IR(2.79×10~4 cells/(ind.·h)) and DR(2.43×10~4 cells/(ind.·h)) on T. weissflogii, and the lowest amounts of ingested P. shikokuense were detected. The highest assimilation rate(~90.64%, IR/DR) was observed in copepods fed with P. shikokuense. The qPCR method used here can help determine the digestion rate and assimilation rate of copepods by detecting cells remaining in the gut hence providing the possibility to examine trophic links involving key species in the marine ecosystem. Our results indicate that A. erythraea has diet-specific feeding performance in different processes, and a quantitative assessment of copepod feeding is needed to accurately determine its functional role in the energy and matter uptake from marine food webs.

关键词： copepod ingestion rate digestion rate 18S rDNA real-time PCR

南沙群岛珊瑚礁区黑斑鹦嘴鱼(Scarus globiceps)食性分析

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热带海洋学报 2024年第3期43卷 100-108页

作者：林先智周岩岩林皓晔胡思敏黄晖张黎刘胜中国科学院热带海洋生物资源与生态重点实验室广东省应用海洋生物学重点实验室中国科学院南海海洋研究所广东广州510301 中国科学院大学北京100049 三亚海洋科学综合(联合)实验室海南省热带海洋生物技术重点实验室三亚中科海洋研究院海南三亚572000 中国科学院海南热带海洋生物实验站海南三亚海洋生态系统国家野外科学观测研究站海南三亚572000 三亚海洋生态环境工程研究院海南三亚572025

鹦嘴鱼科(Scaridae)鱼类参与珊瑚礁生态系统诸多关键生态过程,在维持珊瑚礁生态系统稳定与平衡中发挥着重要作用。由于以往研究手段的限制,对鹦嘴鱼的食物来源及生态功能价值认识不足,在其功能定位方面存在较多争议。本研究选择珊瑚分... 详细信息

鹦嘴鱼科(Scaridae)鱼类参与珊瑚礁生态系统诸多关键生态过程,在维持珊瑚礁生态系统稳定与平衡中发挥着重要作用。由于以往研究手段的限制,对鹦嘴鱼的食物来源及生态功能价值认识不足,在其功能定位方面存在较多争议。本研究选择珊瑚分布的典型区域—南沙群岛中的东门礁和南薰礁为研究海域,对该海域鹦嘴鱼优势种类黑斑鹦嘴鱼(Scarus globiceps)摄食的藻类多样性进行全面分析。通过18S rDNA和16S rDNA多基因条形码技术,分别对黑斑鹦嘴鱼肠含物的真核藻类和原核藻类DNA进行高通量测序分析。18S rDNA的测序结果发现,黑斑鹦嘴鱼类的肠含物中真核藻类以甲藻(Dinoflagellata)、红藻(Rhodophyta)、绿藻(Chlorophyta)、褐藻(Ochrophyta)为主,共计77个OTU(operational taxonomic units)。甲藻相对序列丰度和多样性较高,序列比例占真核藻类序列总数的51.42%,其中网甲藻科(Suessiaceae)OTU_5在东门礁和南薰礁的样品中均超过20%。16S rDNA测序鉴定发现肠含物含有原核藻类(蓝藻)的序列,共计21个OTU,其中以念珠藻目(Nostocales)的相对序列丰度最高,达到39.33%。本研究表明,黑斑鹦嘴鱼在摄食过程中会摄入一定量大型藻类,但微藻(甲藻)序列占据优势地位,蓝藻在肠含物中也有较高的检出率,说明需要重新考虑微藻(甲藻和蓝藻)在鹦嘴鱼食源中的重要贡献以及对珊瑚礁生态系统结构与功能的影响。

关键词：鹦嘴鱼食性分析高通量测序 18SrDNA 16SrDNA 甲藻蓝藻

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Flexible feeding patterns of copepod Centropages tenuiremis in fluctuating conditions: a possible survival strategy to cope with disturbance

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Acta Oceanologica Sinica 2020年第2期39卷 59-68页

作者： Cuilian Xu simin hu Zhiling Guo Tao Li hui huang Leo Lai Chan Sheng Liu Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Provincial Key Laboratory of Applied Marine Biology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China Tropical Marine Biological Research Station in Hainan Chinese Academy of SciencesSanya 572000China Shenzhen Key Laboratory for the Sustainable Use of Marine Biodiversity Research Centre for the Oceans and Human HealthCity University of Hong Kong Shenzhen Research InstituteShenzhen 518057China State Key Laboratory in Marine Pollution City University of Hong KongHong Kong 999077China

Centropages tenuiremis is a species with a wide distribution range in disturbed coastal ***,due to a lack of dietary information,it remains unclear as to how they maintain such dominance in fluctuating *** this study,*** was collected from the Daya Bay Nuclear Power Plant both in inlet and outfall regions at 06:00,12:00 and 18:00 on April 27,2011 and their in situ diet was analyzed using a PCR protocol targeting 18S ribosomal ***-four species of prey organisms were identified totally,including Dinophyta,Baciliariophyta,Viridiplantae,Rhizaria,Apicomplexa,Chordata,Mollusca,Arthropoda and Fungi,indicating an obvious omnivorous feeding habit of *** tenuiremis obviously exhibited spatial and temporal variations in diet *** plant prey(land plants and phytoplankton)were consumed in the morning(~50%),while more animal prey(metazoans and protozoans)were ingested at midday and night(60%–70%).Furthermore,a more diverse diet was detected in the outfall region(10–11 taxa),where the temperatures were relatively higher and more fluctuating,than in the control region(5–10 taxa).This finding indicated that *** could potentially expand its food spectrum under stressful ***,*** exhibited phytoplankton preference(58.62%–67.64%)in the outfall region with a lower omnivory index(0.27–0.35)than in the control region(0.51–0.95).However,phytoplankton density was lower than that in the control region,suggesting a possible herbivorous tendency of *** under elevated temperatures to balance the energy acquirement and feeding *** flexible food choices of *** observed here could effectively buffer environmental fluctuations and might be an important survival strategy in coastal ecosystems.

关键词： Centropages feeding response feeding strategy coastal ecosystem Daya Bay

Mechanisms of high-frequency dinoflagellate blooms of Scrippsiella trochoidea in Daya Bay,South China Sea

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Journal of Oceanology and Limnology 2021年第4期39卷 1293-1304页

作者： Yuan TIAN simin hu Xianzhi LIN hui huANG Xingyu SONG Yan YAN Xuedong XIE Tao LI Sheng LIU Key Laboratory of Tropical Marine Bio-resources and Ecology Guangdong Provincial Key Laboratory of Applied Marine BiologySouth China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China University of Chinese Academy of Sciences Beijing 100049China Tropical Marine Biological Research Station in Hainan Chinese Academy of SciencesSanya 572000China Nansha Marine Ecological and Environmental Research Station Chinese Academy of SciencesGuangzhou 510301China Guangdong Ocean and Fishery Environment Monitoring and Forecasting Center Guangzhou 510222China

Harmful algal blooms(HABs),such as dinoflagellate blooms,have adverse eff ects on coastal water environments,causing seafood contamination and aquaculture economic *** on historical HAB records data(1983-2017)and phytoplankton data(1982-2008),field investigation and laboratory experiments on frequently bloomed dinoflagellate species,we analyzed the possible mechanisms of high frequency dinoflagellate blooms in Daya Bay,South China *** HAB records data show that the proportion of dinoflagellate blooms increased from 15% before 1994 to 77.27% after *** bloom occurred mostly in summer(57.58%)in Yaling Bay(76.92%).The species inducing dinoflagellate blooms tended to be singular over time,with high proportion of Scrippsiella trochoidea(42.86%-88.88%).Laboratory experiment and field investigation of *** bloom showed that this species grew rapidly at 30℃ and 32.1℃(r=0.47,P7 m/s),which disturbed the resting cysts in the sediment and bring them into surface *** into account of environmental parameters,algae source,and atmospheric conditions,the probable mechanism of *** bloom could be *** cysts were resuspended by the spring tide or strong wind,germinated at the bottom layer,reproduced rapidly at higher temperature,and then aggregated in the inner bay(Yaling Bay),resulting the frequent *** *** the influences of global warming and human activities,it should be taken more attention that dinoflagellate blooms might increase along the coastal waters.

关键词： harmful algal blooms dinoflagellate Daya Bay Scrippsiella trochoidea

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Analysis of conductance and interfacial polarisation behaviours of silicone rubber based on frequency domain spectroscopy

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High Voltage 2024年第1期9卷 56-65页

作者： Hao hu Zhidong Jia Xilin Wang Ruiyang Guan Weinan Fan simin Luo Tsinghua Shenzhen International Graduate School Tsinghua UniversityShenzhenChina Guangzhou Power Supply China Southern Power Grid Company Ltd.GuangzhouChina

Silicone rubber(SR)is widely used for cable insulation and operates in multi-stress coupling *** dielectric properties and ageing performance are the essential factors affecting cable *** this study,the frequency domain spectroscopy of unaged SR and SR aged under various conditions is *** dielectric responses before and after ageing are analysed in depth by the conjoint analysis via multiple complex planes(permittivity,conductivity,and modulus).Typical conductivity and interfacial polarisation properties,as well as property changes resulting from thermal and tensile ageing,are *** results show that tensile ageing promotes conductance and interfacial polarisation,while thermal ageing shows the opposite *** on the multi-core model and charge carrier hopping transport model,thermal and tensile ageing mechanisms on the conductance and interfacial polarisation behaviours of SR are *** behaviours exhibit positively correlated changing characteristics,suggesting that the two behaviours may originate from a common *** carrier transport process,promoted by the tensile effect and slightly suppressed by the thermal effect,is the primary factor and considered an important indicator to evaluate the dielectric properties of SR insulation.

关键词： interfacial rubber insulation