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The Protective Effect of Arabinose + Mannose on Mice with Acute Liver Injury Induced by CCl_4 and Its Mechanism

Medicinal Plant 2017年第1期8卷 48-50页

作者： simao huang Ya GAO Houkang CAO Kefeng ZHANG Guilin Medical University

[Objectives] Taking mice with acute liver injury induced by CCl_4 as the model,the effect of arabinose + mannose( w/w = 1∶ 1) on mice with acute liver injury induced by CCl_4 was studied. [Methods]60 experimental mice were selected and then randomly divided into normal control,model group and positive group( bifendate 120 mg/kg),high-dose arabinose + mannose group( 800 mg/kg),middle-dose arabinose + mannose group( 400 mg/kg) and low-dose arabinose + mannose group( 200 mg/kg),each group had 10 mice,which were fed adaptively for 1 week. Except normal control group and model group,each treatment group was given medicine by gavage once a day and lasted for7 days according to the dosage of 20 ml/kg. After the last drug,except normal control group,the mice of other groups were injected 10 ml/kg0. 12% CC14 peanut oil through enterocoelia,thereby establishing acute liver injury model. The mice were fasted but not water for 24 h,after that,blood was sampled from mice eyes,then dissected rapidly. The activities of ALT and AST in the serum were determined,the expression levels of TNF-α,IL-1β and IL-6 from the hepatic tissues were detected by enzyme-linked immunosorbent assay( ELISA); then after HE dye,the changes of liver histopathology were observed. [Results]Compared with CCl_4 model,the activities of ALT and AST from the serum of mice from high-dose and middle dose groups decreased significantly( P < 0. 01); the contents of TNF-α,IL-1β and IL-6 from the hepatic tissues of mice decreased significantly( P < 0. 01); the pathological section showed that the liver injury of mice from the combined drug groups showed alleviating trend to varying degrees,in which the liver injury of mice from the high-dose group was the best. [Conclusions] Arabinose + mannose has an obvious protective effect on mice with acute liver injury induced by CCl_4,and its mechanism may relate to anti-inflammatory.

关键词： Arabinose Mannose CCl4 Acute liver injury Anti-inflammatory

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Protection Effect of Qiwei Jingganling on Carbon Tetrachloride-induced Acute Liver Injury in Mice

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Medicinal Plant 2018年第3期9卷 44-46,50页

作者： Boting XI Houkang CAO simao huang Yuman GUAN Kefeng ZHANG Guilin Medical University

[Objectives] The aim was to study the protection effect of Qiwei Jingganling on carbon tetrachloride-induced acute liver injury in mice and its mechanism of action. [Methods]Total 60 mice were randomly and evenly divided into 6 groups,normal group,model group,silymarin group( 150 mg/kg),high-dose Qiwei Jingganling group( 8 g/kg,crude drug),middle-dose Qiwei Jingganling group( 4 g/kg,crude drug) and low-dose Qiwei Jingganling group( 2 g/kg,crude drug). The mice were administered orally once a day according to the amount of10 m L/kg,and 10-day continuous administration was carried out. After 2 h of the last administration,0. 12% CCl4 peanut oil solution( 10 m L/kg) was injected intraperitoneally to all the mice except those in the normal group to establish acute liver injury model. After 16 h,the blood of the mice was collected from the eyeballs,and their liver tissues were collected. The levels of alanine aminotransferase( ALT),aspartate aminotransferase( AST),superoxide dismutase( SOD),malondialdehyde( MDA) and glutathione peroxidase( GSH-Px) in the sera were determined by biochemical methods,and the contents of tumor necrosis factor-α( TNF-α),interleukin-1β( IL-1β) and interleukin-6( IL-6) in the liver tissues were determined with enzyme-linked immunosorbent assays( ELISA). [Results]Qiwei Jingganling significantly reduced the activities or content of ALT,AST and MDA in serum of mice with liver injury( P < 0. 05,P < 0. 01),increased the activities of SPD and GSH-Px( P < 0. 05,P < 0. 01) and down-regulated the expression levels of IL-1β,IL-6 and TNF-α in liver tissue( P < 0. 05,P < 0. 01).[Conclusions]Qiwei Jingganling has a good protection effect on CCl4-induced acute liver injury in mice,which may be related to the inhibition of oxidative stress and inhibition of inflammatory responses.

关键词： Qiwei Jingganling Acute liver injury Carbon tetrachloride Oxidative stress Inflammatory response

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Optimization of Extraction Process of Total Flavonoids from Akebia trifoliata (Thunb.) Koidz. by Central Composite Design-Response Surface Methodology

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Medicinal Plant 2017年第2期8卷 15-18页

作者： Houkang CAO Boting XI simao huang Xiaohua PANG Kefeng ZHANG Guilin Medical University

[Objectives]To optimize extraction process of total flavonoids from Akebia trifoliata( Thunb.) Koidz.,so as to provide references for development and use of Akebia trifoliata( Thunb.) Koidz. [Methods]The extraction rate of total flavonoids of Akebia trifoliata( Thunb.)Koidz. was taken as observation indicator. On the basis of single factor experiment,central composite design( CCD) was used to evaluate the effects of the extraction temperature,extraction time,and ethanol concentration on the extraction process. Multiple linear regression and binomial fitting were used,and response surface methodology( RSM) was used to select the optimum extraction process. [Results] The optimum extraction process conditions for total flavonoids of Akebia trifoliata( Thunb.) Koidz. was extraction temperature: 83. 92 ℃; extraction time:96. 47 min; ethanol concentration: 63. 92%; extraction times: two times; solid to liquid ratio: 1 ∶ 20; extraction rate of total flavonoids:4. 55%. [Conclusions] The central composite design-response surface methodology( CCD-RSM) is simple,convenient,and feasible for extraction of total flavonoids from Akebia trifoliata( Thunb.) Koidz.,and the prediction results are reliable.

关键词： Central composite design-response surface methodology(CCD-RSM) Akebia trifoliata(Thunb.) Koidz. Total flavonoids Extraction process

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Optimization of Extraction Process of Clerodendrum philippinum Schauer var. simplex Mlodenke Total Flavonoids(CPTF) by Central Composite Design-Response Surface Methodology

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Medicinal Plant 2018年第3期9卷 17-20页

作者： Bo LI simao huang Xiaohua PANG Boting XI Houkang CAO Kefeng ZHANG R & D Center of Gansu Tasly Zhongtian Pharmaceutical Co. Ltd. Pulmonary Hospital of Lanzhou Gansu University of Chinese Medicine Guilin Medical University

[Objectives] The research aimed to optimize extraction process of Clerodendrum philippinum Schauer var. simplex Mlodenke total flavonoids( CPTF),and provide reference for its development and utilization. [Methods] Based on single-factor test,ethanol concentration,extraction temperature and extraction time were taken as independent variables,and total flavonoids yield was taken as dependent variable. The test was conducted according to central composite design principle. Multivariate linear regression and binomial equation fitting of the result were conducted,and extraction process of CPTF was optimized by using response surface methodology. [Results]The optimal extraction process of CPTF was as below: ethanol concentration 54. 76%,extraction temperature 83. 92℃,extraction time 102. 64 min,solid-liquid ratio 1:20,extraction for twice. [Conclusions] The extraction process of CPTF by central composite design-response surface methodology was simple and feasible,with reliable prediction result,which was suitable for industrial production.

关键词： Central composite design-response surface methodology Guoqiangfeng Total flavonoids Extraction process

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狗肝菜多糖对二甲基亚硝胺诱导的肝纤维化大鼠TGF-β1/Smads信号通路的影响

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中药药理与临床 2016年第6期32卷 113-117页

作者：高雅张可兰黄思茂曹后康张可锋桂林医学院桂林541004 甘肃省中医院兰州730050

目的:以二甲基亚硝胺(DMN)诱导的大鼠肝纤维化为模型,研究狗肝菜多糖对DMN诱导的肝纤维化大鼠TGF-β1/Smads信号通路的影响。方法:将96只大鼠随机分为对照组、模型组、狗肝菜多糖(300 mg/kg,200 mg/kg,100 mg/kg)组和秋水仙碱组(0.12 mg... 详细信息

目的:以二甲基亚硝胺(DMN)诱导的大鼠肝纤维化为模型,研究狗肝菜多糖对DMN诱导的肝纤维化大鼠TGF-β1/Smads信号通路的影响。方法:将96只大鼠随机分为对照组、模型组、狗肝菜多糖(300 mg/kg,200 mg/kg,100 mg/kg)组和秋水仙碱组(0.12 mg/kg)。除对照组以外,其余各组大鼠均腹腔注射0.5%DMN溶液(1.5 ml/kg),隔天1次,在首次注射DMN同时,各组大鼠灌胃给予相应的药物,而正常组和模型组灌胃给予相应体积的生理盐水溶液,每天给药1次,连续5周,5周末眼球取血,脱臼致死,收取肝脏。生化法检测血清中碱性磷酸酶(ALP)、谷氨酰氨基转移酶(GGT)水平;酶联免疫法检测(ELISA)血清中透明质酸(HA)、层黏连蛋白(LN)、Ⅲ型前胶原(PCⅢ)和Ⅳ型胶原(Ⅳ-C)含量。苏木精-伊红染色(HE染色)进行肝组织病理学检查;蛋白质印迹法(Western blot)检测肝组织a-平滑肌肌动蛋白(a-SMA)、转化生长因子β1(TGF-β1)和Smad2/3/7的蛋白表达情况。结果:与模型组比较,经狗肝菜多糖(300 mg/kg,200 mg/kg,100 mg/kg)组干预后,血清中ALP、GGT、HA、LN、PCⅢ和Ⅳ-C水平显著降低;肝组织中a-SMA、TGF-β1、Smad2/3的蛋白表达被抑制,而Smad7的表达得到促进;病理切片显示肝纤维化大鼠肝组织炎症坏死程度和肝纤维化病变程度较模型组明显减轻。结论:狗肝菜多糖具有抗DMN诱导的大鼠肝纤维化的作用,其机制与TGF-β1/Smads信号通路相关因子的表达有关。

关键词：狗肝菜多糖肝纤维化 TGF-β1/Smads 信号通路

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金花茶多糖对四氯化碳致小鼠急性肝损伤的保护作用及其机制的研究

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中药药理与临床 2016年第6期32卷 117-120页

作者：黄思茂曹后康高雅蒋志敏张可锋桂林医学院桂林541004

目的:研究金花茶多糖对四氯化碳(CCl_4)诱发的小鼠急性肝损伤的保护作用及其作用机制。方法:60只小鼠随机分为正常对照组、模型组、联苯双酯组(120 mg/kg)和金花茶多糖组(200 mg/kg,100 mg/kg,50 mg/kg)。除正常对照组及模型组外,各治... 详细信息

目的:研究金花茶多糖对四氯化碳(CCl_4)诱发的小鼠急性肝损伤的保护作用及其作用机制。方法:60只小鼠随机分为正常对照组、模型组、联苯双酯组(120 mg/kg)和金花茶多糖组(200 mg/kg,100 mg/kg,50 mg/kg)。除正常对照组及模型组外,各治疗组按20 ml/kg剂量灌胃给药,每天按时灌胃1次,共7天。末次给药后,除正常对照组外,其余组腹腔注射0.12%CCl_4花生油(10 ml/kg)建立急性肝损伤模型。禁食不禁水24 h后,眼球取血,收集肝脏。生化法测定血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性或含量;酶联免疫吸附法(ELISA)检测肝组织中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)表达水平和一氧化氮(NO)含量;蛋白质印迹法(Western blot)检测肝组织NF-κB蛋白表达情况;HE染色肝切片,显微镜观察肝组织病理改变。结果:与CCl_4模型组相比,金花茶多糖剂量组(200mg/kg,100 mg/kg)小鼠血清中ALT、AST及MDA含量降低,SOD及GSH-Px的活性升高;肝组织中NO含量显著降低;肝组织中TNF-α、IL-1β、IL-6及NF-κB表达水平呈下降趋势;病理切片表明金花茶多糖剂量组(200 mg/kg,100 mg/kg)小鼠肝损伤显著减轻。而金花茶多糖50 mg/kg剂量组对CCl_4诱发的小鼠急性肝损伤无明显改善作用。结论:金花茶多糖对CCl_4诱导的小鼠急性肝损伤有明显的保护作用,其机制可能与抗氧化应激,清除自由基代谢产物,抑制脂质过氧化反应及抗炎有关。

关键词：金花茶多糖四氯化碳急性肝损伤氧化应激

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火炭母总黄酮对D-氨基半乳糖诱导的肝损伤小鼠的保护作用及其机制

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中药药理与临床 2018年第2期34卷 32-35页

作者：黄思茂王梦楠许琼梅王欢张可锋桂林医学院桂林541004 甘肃中医药大学兰州730000

目的:研究火炭母总黄酮对D-氨基半乳糖(D-Gal N)建立的急性肝损伤小鼠的保护作用及机制。方法:将60只昆明种小鼠随机分为正常对照组,模型组,水飞蓟素组(200 mg/kg)及火炭母总黄酮剂量组(400 mg/kg、200 mg/kg、100 mg/kg),每组10只,适... 详细信息

目的:研究火炭母总黄酮对D-氨基半乳糖(D-Gal N)建立的急性肝损伤小鼠的保护作用及机制。方法:将60只昆明种小鼠随机分为正常对照组,模型组,水飞蓟素组(200 mg/kg)及火炭母总黄酮剂量组(400 mg/kg、200 mg/kg、100 mg/kg),每组10只,适应性喂养一周后,各给药组小鼠每天灌胃给药1次,连续7天,正常对照组及模型组小鼠灌胃生理盐水,末次给药1 h后,除正常对照组,其余组均腹腔注射D-Gal N(500 mg/kg)建立小鼠急性肝损伤模型。模型建立16 h后,收集血清及肝组织,生化法检测小鼠血清中AST、ALT、TBIL、T-SOD、MDA和GSH-Px的活性或含量;酶联免疫吸附法(ELISA)检测小鼠肝组织中Th1型细胞因子TNF-α与IFN-γ以及Th2型细胞因子IL-10与白IL-4的表达水平;HE染色,显微镜下观察小鼠肝组织病理变化。结果:与正常对照组相比较,模型组小鼠血清中AST、ALT和TBIL水平显著升高,肝组织切片显示,模型组小鼠肝组织严重受损,肝细胞明显坏死,提示肝损伤模型建立成功。与模型组相比,火炭母总黄酮剂量组(400 mg/kg和200 mg/kg)小鼠血清或肝组织中AST、ALT、TBIL、MDA、TNF-α和IFN-γ水平不同程度显著下降,而T-SOD、GSH-Px、IL-10和IL-4水平显著升高;HE染色结果显示,火炭母总黄酮能够显著改善肝损伤病变程度。结论:火炭母总黄酮具有显著的护肝作用,其作用机制可能与抗氧化及调节肝组织Th1/Th2细胞平衡有关。

关键词：火炭母总黄酮 D-氨基半乳糖(D-GalN) 急性肝损伤抗氧化 Th1/Th2细胞平衡

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星点设计-效应面法优化过墙风总黄酮的提取工艺

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中兽医医药杂志 2018年第2期37卷 5-8页

作者：黄思茂庞小华葸博婷曹后康张可锋桂林医学院广西桂林541004 甘肃中医药大学甘肃兰州730000

目的：优化瑶药过墙风总黄酮提取工艺,为其开发和利用提供参考。方法：在单因素试验筛选基础上,以乙醇浓度、提取温度和提取时间为自变量,总黄酮得率为因变量,然后根据星点设计原理进行试验,对结果进行多元线性回归和二项式方程拟合,采... 详细信息

目的：优化瑶药过墙风总黄酮提取工艺,为其开发和利用提供参考。方法：在单因素试验筛选基础上,以乙醇浓度、提取温度和提取时间为自变量,总黄酮得率为因变量,然后根据星点设计原理进行试验,对结果进行多元线性回归和二项式方程拟合,采用效应面法优化过墙风总黄酮的提取工艺。结果：过墙风总黄酮最优提取工艺为乙醇浓度54.76%,提取温度83.92℃,提取时间102.64 min,固液比为1∶20,提取2次。结论：本实验星点设计-效应面法优化过墙风总黄酮提取工艺简便可行,预测结果可靠,适于工业化生产。

关键词：星点设计-效应面法过墙风总黄酮提取工艺

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湍流模型对瞬态流固耦合换热数值模拟影响分析

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原子能科学技术 2019年第2期53卷 304-311页

作者：丁文杰郭海兵黄洪文郭斯茂中国工程物理研究院核物理与化学研究所四川绵阳621900

采用ANSYS Fluent程序建立了固体加热功率指数增长的气体冷却模型,分析了瞬态过程中的流动传热特性,通过数值模拟结果与3组实验数据进行对比,研究了标准k-ε模型、标准k-ω模型、过渡SST模型和RSM模型4种典型湍流模型对瞬态过程中流固... 详细信息

采用ANSYS Fluent程序建立了固体加热功率指数增长的气体冷却模型,分析了瞬态过程中的流动传热特性,通过数值模拟结果与3组实验数据进行对比,研究了标准k-ε模型、标准k-ω模型、过渡SST模型和RSM模型4种典型湍流模型对瞬态过程中流固耦合换热数值模拟结果的影响。通过分析发现:热负载指数提升过程中,热功率一部分用于对流换热,而另一部分仍留在固体内,且热功率提升速率越高,对流换热的占比越低;采用不同湍流模型模拟出的等效表面热流密度均低于实验值,且采用ε类湍流模型的模拟结果与实验数据更接近。

关键词：湍流模型瞬态流固耦合换热数值模拟

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Genesis of Xinzhai Sandstone-Type Copper Deposit in Northern Laos: Geological and Geochemical Evidences

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Journal of Earth Science 2019年第1期30卷 95-108页

作者： Jianguo huang Tao Ren Haijun Zou Faculty of Land Resources Engineering Kunming University of Science and Technology State Key Laboratory of Ore Deposit Geochemistry Institute of Geochemistry Chinese Academy of Sciences

Xinzhai sandstone-type copper deposit located in northern Laos lies in the Jiangcheng-Phongsaly-Phrae Mesozoic basin(JPMB), which is regarded as southern extension of the Lanping-simao Mesozoic basin in China. The copper deposit belt is bounded by the AilaoshanHeishui River fault and the Dian Bien Phu-Luang Prabang fault at the east and Lancang RiverBannankan faults at the west. Two types of orebodies are identified in the Xinzhai area based on geological investigation. One is lamellar copper orebody hosted by the fine lithic feldspar sandstones and feldspar lithic sandstones; another is vein-type orebody. The sulfur isotopic compositions of the chalcopyrite and tetrahedrite are from -11.6‰ to -1.8‰, indicating that sulfur is derived from bacterial sulfate reduction(BSR). δD values of fluid inclusions in ore-bearing quartz samples are from -99‰ to -78‰. The calculated δ^(18)OH_2O values of ore-forming fluid vary from -2.3‰ to 0.4‰ using the quartz-water fractionation equations and the mineralization temperature. Oxygen and hydrogen isotopic compositions show that the ore-forming fluid was derived from basin fluid. Rock-mineral identifications show that both of the mineral grain maturity and the structural maturity are high in the Jurassic Huakaizuo Formation, reflecting a far-source accumulation and lake facies sedimentatary environment. Based on tectonic determination diagram of the Al_2O_3/SiO_2-TFe+MgO, the sandstone samples collected from the Huakaizuo Formation were plotted in the passive continental margin. The collision of the Indian and Eurasian blocks during the Cenozoic formed large-scale strikeslips and thrust nappe structures in margin of the basin. With the tectonic movement, Cu-rich basin fluid from the basement of basin migrated upward along the contemporaneous fault and into the high porosity strata. At the same time, in organic matter-riched condition, bacterial sulfate reduction(BSR) has been triggered, forming a large number of S^(2-) ions, and th

关键词： Laos sandstone-type copper deposit geology geochemistry genesis

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