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Characterization of Leaf Transcriptome in Banksia hookeriana

Genomics, Proteomics & Bioinformatics 2017年第1期15卷 49-56页

作者： sim lin lim Haylee M.D'Agui Neal J.Enright Tianhua He Department of Environment and Agriculture Curtin UniversityPerthWA 6845Australia School of Veterinary and Life Sciences Murdoch UniversityPerthWA 6150Australia

Abstract Banksia is a significant element in vegetation of southwestern Australia, a biodiversity hotspot with global significance. In particular, Banksia hookeriana represents a species with significant economic and ecological importance in the region. For better conservation and management, we reported an overview of transcriptome of B. hookeriana using RNA-seq and de novo assembly. We have generated a total of 202.7 million reads （18.91 billion of nucleotides） from four leaf sam- ples in four plants of B. hookeriana, and assembled 59,063 unigenes （average size = 1098 bp） through de novo transcriptomc assembly. Among them, 39,686 unigenes were annotated against the Swiss-Prot, Clusters of Orthologous Groups （COG）, and NCBI non-redundant （NR） protein databases. We showed that there was approximately one single nucleotide polymorphism （SNP） per 5.6-7.1 kb in the transcriptome, and the ratio of transitional to transversional polymorphisms was approximately 1.82. We compared unigenes of B. hookeriana to those of Arabidopsis thaliana and Nelumbo nucifera through sequence homology, Gene Ontology （GO） annotation, and KEGG pathway analyses. The comparative analysis revealed that unigenes of B. hookeriana were closely related to those of N. nucifera. B. hookeriana, N. nucifera, and A. thaliana shared similar GO anno- tations but different distributions in KEGG pathways, indicating that B. hookeriana has adapted to dry-Mediterranean type shrublands via regulating expression of specific genes. In total 1927 potential simple sequence repeat （SSR） markers were discovered, which could be used in the genotype and genetic diversity studies of the Banksia genus. Our results provide valuable sequence resource for further study in Banksia.

关键词： Banksia hookeriana Gene annotation RNA-seq SSR marker Transcriptome

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Biomimetic Fe_(7)S_(8)/Carbon electrocatalyst from[FeFe]-Hydrogenase for improving pH-Universal electrocatalytic hydrogen production Special Collection:Aggregation-Induced Processes and Functions

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Aggregate 2024年第1期5卷 451-459页

作者： Dohun Kim Subramani Surendran Sejin Im Jaehyoung lim Kyoungsuk Jin Ki Tae Nam Uk sim Department of Energy Science&Engineering Daegu Gyeongbuk Institute of Science&Technology(DGIST)DaeguRepublic of Korea Hydrogen Energy Technology Laboratory Korea Institute of Energy Technology(KENTECH)NajuRepublic of Korea Department of Chemistry Korea UniversitySeoulRepublic of Korea Department of Materials Science and Engineering Seoul National UniversitySeoulRepublic of Korea

Efficient and cost-effective electrocatalysts that can operate across a wide range of pH conditions are essential for green hydrogen *** by biological systems,Fe_(7)S_(8)nanoparticles incorporated on polydopamine matrix electrocatalyst were synthesized by co-precipitation and annealing *** resulting Fe_(7)S_(8)/C electrocatalyst possesses a three-dimensional structure and exhibits enhanced electrocatalytic performance for hydrogen production across various pH ***,the Fe_(7)S_(8)/C electrocatalyst demonstrates exceptional activity,achieving low overpotentials of 90.6,45.9,and 107.4 mV in acidic,neutral,and alkaline environments,*** impedance spectroscopy reveals that Fe_(7)S_(8)/C exhibits the lowest charge transfer resistance under neutral conditions,indicating an improved proton-coupled electron transfer ***-wave electron paramagnetic resonance results confirm a change in the valence state of Fe from 3+to 1+during the hydrogen evolution reaction(HER).These findings closely resemble the behavior of natural[FeFe]-hydrogenase,known for its superior hydrogen production in neutral *** remarkable performance of our Fe_(7)S_(8)/C electrocatalyst opens up new possibilities for utilizing bioinspired materials as catalysts for the HER.

关键词： biomimetic electrocatalyst hydrogen production renewable energy

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Application of artifcial intelligence in cataract management:current and future directions

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Eye and Vision 2024年第1期10卷 21-30页

作者： Laura Gutierrez Jane Sujuan lim Li Lian Foo Wei Yan Ng Michelle Yip Gilbert Yong San lim Melissa Hsing Yi Wong Allan Fong Mohamad Rosman Jodhbir Singth Mehta Haotian lin Darren Shu Jeng Ting Daniel Shu Wei Ting Singapore Eye Research Institute SingaporeSingapore Singapore National Eye Center 11 Third Hospital AvenueSingapore 168751Singapore Academic Ophthalmology School of MedicineUniversity of NottinghamNottinghamUK Zhongshan Ophthalmic Center Sun Yet Sen UniversityGuangzhouChina

The rise of artifcial intelligence(AI)has brought breakthroughs in many areas of *** ophthalmology,AI has delivered robust results in the screening and detection of diabetic retinopathy,age-related macular degeneration,glaucoma,and retinopathy of *** management is another feld that can beneft from greater AI *** is the leading cause of reversible visual impairment with a rising global clinical *** diagnosis,monitoring,and surgical management are necessary to address this *** addition,patients in large developing countries often sufer from limited access to tertiary care,a problem further exacerbated by the ongoing COVID-19 *** on the other hand,can help transform cataract management by improving automation,efcacy and overcoming geographical ***,AI can be applied as a telediagnostic platform to screen and diagnose patients with cataract using slit-lamp and fundus *** utilizes a deep-learning,convolutional neural network(CNN)to detect and classify referable cataracts ***,some of the latest intraocular lens formulas have used AI to enhance prediction accuracy,achieving superior postoperative refractive results compared to traditional ***,AI can be used to augment cataract surgical skill training by identifying diferent phases of cataract surgery on video and to optimize operating theater workfows by accurately predicting the duration of surgical ***,some AI CNN models are able to efectively predict the progression of posterior capsule opacifcation and eventual need for YAG laser *** advances in AI could transform cataract management and enable delivery of efcient ophthalmic *** key challenges include ethical management of data,ensuring data security and privacy,demonstrating clinically acceptable performance,improving the generalizability of AI models across heterogeneous populations,and improving the trust of e

关键词： Artifcial intelligence Telemedicine Cataract Cataract screening Cataract surgery IOL calculations Biometry Machine learning

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Retinal Vascular Imaging Application in Women’s Reproductive Health:Clinical Implications and Future Directions

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Maternal-Fetal Medicine 2024年第2期6卷 92-96页

作者： Beth Shi Yu lim ling-Jun Li Yong Loo lin School of Medicine National University of SingaporeSingapore 117597Singapore Department of Obstetrics&Gynaecology Yong Loo Lin School of MedicineNational University of SingaporeSingapore 119228Singapore Global Centre for Asian Women’s Health Human Potential Translational Research ProgrammeYong Loo Lin School of MedicineNational University of SingaporeSingapore 117597Singapore NUS Bia-Echo Asia Centre for Reproductive Longevity and Equality(ACRLE) Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117456Singapore Singapore Eye Research Institute Singapore National Eye CentreSingapore 168751Singapore

Over the past two decades,population-based studies employing semiautomatic computer-assisted programs have uncovered associations between retinal microvascular features and various systemic *** the recognition of retinal imaging in cardiometabolic health grows,there is increasing evidence supporting its application in women’s health,particularly during the reproductive *** review aims to summarize the indications of retinal imaging in women’s health and intergenerational health,where suboptimal retinal imaging has been found to mirror pathological systemic changes,such as suboptimal hemodynamic circulation,inflammation,endothelial dysfunction,oxidative stress,and hypoxia in *** from Singapore Growing Up in Singapore Towards Healthy Outcomes and Singapore Preconception Study of Long-Term Maternal and Child Outcomes cohorts have reported serial changes in retinal conventional microvascular features(e.g.,retinal arteriolar narrowing,retinal venular widening)and retinal geometric microvascular features(e.g.,sparse fractal dimension,enlarged branching angle,and increased curvature tortuosity)during the preconception and antenatal *** morphological abnormalities were found to be related to female fertility,maternal antenatal health conditions,postnatal maternal cardiometabolic health,and intergenerational health in the *** the compelling evidence of the ability to detect microvascular changes through noninvasive methods at an early stage,retinal imaging holds the potential to facilitate timely interventions,mitigate the progression of complications,and prevent adverse pregnancy *** ahead,the convergence of artificial intelligence and advanced imaging techniques heralds a promising era in women’s health research and clinical practice.

关键词： Retinal vascular imaging Reproductive health

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Effects of the windshield inclination angle on head/brain injuries in car-to-pedestrian collisions using computational biomechanics models

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Transportation Safety and Environment 2024年第2期6卷 1-12页

作者： Fang Wang Mingliang Wang lin Hu Ke Peng Jiajie Yin Danqi Wang Liangliang Shi Zhou Zhou School of Automotive and Mechanical Engineering Changsha University of Science and TechnologyChangsha 410114HunanChina Hunan Province Key Laboratory of Safety Design and Reliability Technology for Engineering Vehicle Changsha University of Science and TechnologyChangsha 410114HunanChina State Key Laboratory of Vehicle NVH and Safety Technology China Automotive Engineering Research Institute Co.Ltd.Chongqing 401122China Division of Neuronic Engineering KTH Ro y al Institute of TechnologyStockholm 14152Sweden

Car-to-pedestrian collision(CPC)accidents occur frequently,and pedestrians often suffer serious head/brain *** major cause is the primary impact with the ***,we use an umerical sim ulation method to study the influence of the windshield in-clination angle of a passenger car on pedestrian head/brain injury due to CPC *** range of the windshield inclination angle was set at 24°-50°,with an interval of 2°.The results show that the windshield angle significantly affects the pedestrian kine-matics and exerts different effects on the head injury when evaluating with various head injury *** the head peak linear/rotational acceleration and acceleration-based head injury criterion(HIC)/rotational injury criterion(RIC),the predictions at the secondary impact stage have no clear relationship with the windshield angle(R^(2)=0.04,0.07,0.03 and 0.26,respectively)and their distributions are *** the primary impact,the peak linear acceleration and HIC show a weak trend of first decreasing and then increasing with the increasing of the windshield angle,and the rotational acceleration and RIC tend to remain relatively *** the cum ulative strain dama ge measure(CSDM)criterion,the predictions at the primary impact are slightly lower than those at the secondary impact,and the trend of first decreasing and then increasing with the increase in the windshield angle is observed at both impact *** the windshield inclination angle is approximately 32°-40°,the head injury severity in both impact phases is generally lower than that predicted at other windshield angles.

关键词： car-to-pedestrian collision(CPC) head/brain injury head injury criterion(HIC) windshield inclination angle computa-tional biomechanics model(CPCBM)

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Single crystal growth and transport properties of narrow-bandgap semiconductor RhP_(2)

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Chinese Physics B 2024年第8期33卷 545-549页

作者：吴德胜郑萍雒建林 Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China Songshan Lake Materials Laboratory Dongguan 523808China Quantum Science Center of Guangdong–Hong Kong–Macao Greater Bay Area(Guangdong) Shenzhen 518045China

We report the growth of high-quality single crystals of RhP_(2),and systematically study its structure and physical properties by transport,magnetism,and heat capacity ***-crystal x-ray diffraction reveals that RhP_(2) adopts a monoclinic structure with the cell parameters a=5.7347(10)A,b=5.7804(11)A,and c=5.8222(11)A,space group P2_(1)/c(No.14).The electrical resistivityρ(T)measurements indicate that RhP_(2) exhibits narrow-bandgap behavior with the activation energies of 223.1 meV and 27.4 meV for two distinct regions,*** temperaturedependent Hall effect measurements show electron domain transport behavior with a low charge carrier *** find that RhP_(2) has a high mobilityμ_(e)~210 cm^(2)·V^(-1)·s^(-1)with carrier concentrations n_(e)~3.3×10^(18)cm^(3) at 300 K with a narrow-bandgap *** high mobilityμ_(e) reaches the maximum of approximately 340 cm^(2)·V^(-1)·s^(-1)with carrier concentrations n_^(e)~2×10^(18)cm^(-3)at 100 *** magnetic phase transitions are observed from the susceptibilityχ(T)and specific heat C_(p)(T)measurements of RhP_(2).Our results not only provide effective potential as a material platform for studying exotic physical properties and electron band structures but also motivate further exploration of their potential photovoltaic and optoelectronic applications.

关键词： single crystal growth narrow band system electrical transport high mobilities

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A review on the mechanisms of low salinity water/surfactant/nanoparticles and the potential synergistic application for c-EOR

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Petroleum Research 2023年第3期8卷 324-337页

作者： Stanley sim Sze lim Henry Elochukwu Jobrun Nandong Ziad Bennour Mohamed Ali Hamid Chemical and Energy Department Faculty of Engineering and ScienceCurtin University MalaysiaMalaysia School of Engineering and Physical Sciences Heriot Watt UniversityMalaysia

Chemical enhanced oil recovery(c-EOR)is a conventional and promising strategy to recover oil from reservoir techniques such as low salinity water flooding(LSWF),surfactant flooding,alkaline flooding,polymers flooding,and nanofluid *** use of various types of chemical materials for c-EOR method has recently attracted the attention of the oil and gas *** primary objective of this review work is to explore the synergy of low salinity water/surfactant/nanoparticle flooding for effective c-EOR method and investigate the mechanism behind these *** advantages of combining these chemical materials for c-EOR methods is also *** and limitations of this synergy and their economic feasibility for additional oil recovery and potential return on investment are *** have been successfully used in various applications in several industries and have also shown good application for EOR in terms of wettability *** contributes to wettability alteration,while surfactant contributes to wettability alteration and interfacial tension(IFT)***,fines migration caused by LSWF and nanoparticle agglomeration can cause formation damage,while excessive surfactant adsorption can lead to cost overrun on surfactant *** the characteristics of reservoir formation mineralogy and appropriate nanoparticle type,size,and concentration can be used to resolve this *** synergy of LSWF and nanoparticles in alkaline medium can serve as sacrificial agent to reduce excessive surfactant ***,the appropriate synergistic formulation of LSFW/surfactant/nanoparticle can improve additional oil recovery and support return on investment for c-EOR projects.

关键词： Surfactant Low salinity water Nanoparticles Nanofluid Enhanced oil recovery

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Accurate diagnosis of severe coronary stenosis based on resting magnetocardiography: a prospective, single-center, cross-sectional analysis

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Journal of Geriatric Cardiology 2024年第4期21卷 407-420页

作者： Jian-Guo CUI Feng TIAN Yu-Hao MIAO Qin-Hua JIN Ya-Jun SHI Li LI Meng-Jun SHEN Xiao-Ming XIE Shu-lin ZHANG Yun-Dai CHEN School of Medicine Nankai UniversityTianjinChina Senior Department of Cardiology the Sixth Medical CenterChinese PLA General HospitalBeijingChina Joint Laboratory of Bioimaging Technology and Applications SAS-SIM-IT&MEDIShanghaiChina Shanghai Institute of Microsystem and Information Technology Chinese Academy of SciencesShanghaiChina

OBJECTIVE To evalu ate the role of resting magnetocardiography in identifying seve re coronary artery stenosis in patients with suspected coronary artery *** A total of 513 patients with angina symptoms were included and divided into two groups based on the extent of coronary artery disease determined by angiography:the non-severe coronary stenusis group(linical *** performance of the models was evaluated using receiver operating characteristic curves,accuracy,sensitivity,specificity,positive predictive value(PPV) and ne gative predictive value(NPV).Calibration plots and decision curve analysis were performed to investigate the clinical utility and performance of the models,*** In the severe coronary stenosis group,QR_MCTDd,S_MDp,and TT_(MA)C_(50) were significantly higher than those in the non-severe coronary stenosis group(10,46±10.66 vs,5.11±6.07,P <0.001;7.2±8.64 vs.4.68±6.95,P=0.003;0.32±57.29 vs.0.26±57.29,P <0.001).While,QR_MV_(amp),R_(MA),and T_(MA) in the severe coronary stenosis group were lower(0.23±0.16 vs.0.28±0.16,P<0.001;55.06±48.68 vs.59.24±53.01,P<0.001;51.67±39.32 vs. 60.45±51.33,P <0.001).Seven MFM parameters were integrated into the model,resulting in an area under the curve of 0.810(95% CI:0.765-0.855).The sensitivity,specificity,PPV,NPV,and accurecy were 71.7%,80.4%,93.3%,42.8 %,and 73.5%;*** combined model exhibited an area under the curve of 0.845(95% CI:0.798-0.892).The sensitivity,specificity,PPV,NPV,and accuracy were 84.3%,73.8%,92.6%,54.6%,and 82.1%;*** curves demonstrate d excellent agreement between the nomogram prediction and actual *** decision curve analysis showed that the c ombine d model provided greater net benefit compared to the magnetocardingraphy model.C

关键词： coronary stenosis specificity

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Targeting pancreatic cancer immune evasion by inhibiting histone deacetylases

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World Journal of Gastroenterology 2022年第18期28卷 1934-1945页

作者： Wynne sim Wei-Meng lim ling-Wei Hii Chee-Onn Leong Chun-Wai Mai School of Medicine International Medical UniversityKuala Lumpur 57000Malaysia School of Pharmacy International Medical UniversityKuala Lumpur 57000Malaysia Center for Cancer and Stem Cell Research Institute for ResearchDevelopmentand InnovationInternational Medical UniversityKuala Lumpur 57000Malaysia AGTC Genomics Kuala Lumpur 57000Malaysia State Key Laboratory of Oncogenes and Related Genes Renji-Med X Clinical Stem Cell Research CenterRen Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of Pharmaceutical Chemistry Faculty of Pharmaceutical SciencesUCSI UniversityKuala Lumpur 56000Malaysia

The immune system plays a vital role in maintaining the delicate balance between immune recognition and tumor ***,it is not uncommon that cancerous cells can intelligently acquire abilities to bypass the antitumor immune responses,thus allowing continuous tumor growth and *** evasion has emerged as a significant factor contributing to the progression and immune resistance of pancreatic *** with other cancers,pancreatic cancer has a tumor microenvironment that can resist most treatment modalities,including emerging ***,the use of immunotherapy has yet to bring significant clinical breakthrough among pancreatic cancer patients,suggesting that pancreatic cancer has successfully evaded *** this review,we summarize the impact of genetic alteration and epigenetic modification(especially histone deacetylases,HDAC)on immune evasion in pancreatic *** overexpression significantly suppresses tumor suppressor genes,contributing to tumor growth and *** review the evidence on HDAC inhibitors in tumor eradication,improving T cells activation,restoring tumor immunogenicity,and modulating programmed death 1 *** provide our perspective in targeting HDAC as a strategy to reverse immune evasion in pancreatic cancer.

关键词： Histone acetylation Histone deacetylases inhibitors Immune evasion Pancreatic cancers Pancreatic ductal adenocarcinoma

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基于遥感数据与数值模式的浒苔漂移输运预报:以江苏近海为例

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海洋学研究 2024年第1期42卷 69-82页

作者：林连杰董昌明嵇宇翔 lim KAM SIAN Kenny Thiam Choy 李兆鑫蒋星亮曹玉晗高慧王胜强曹茜南京信息工程大学海洋科学学院江苏南京210044 江苏省连云港市气象局江苏连云港222006 无锡学院江苏无锡214105 华东师范大学河口海岸学国家重点实验室上海200241 复旦大学大气与海洋科学系上海200433 江苏海洋大学海洋技术与测绘学院江苏连云港222005

浒苔暴发是影响全球近岸海域最为严重的海洋灾害之一,引起了社会各方的重视。该研究基于大气-波浪-海洋集合模式,根据卫星遥感数据提取浒苔位置,采用拉格朗日漂流模式对浒苔进行追踪并预报其漂移路径。与卫星观测结果对比表明,该集合模... 详细信息

浒苔暴发是影响全球近岸海域最为严重的海洋灾害之一,引起了社会各方的重视。该研究基于大气-波浪-海洋集合模式,根据卫星遥感数据提取浒苔位置,采用拉格朗日漂流模式对浒苔进行追踪并预报其漂移路径。与卫星观测结果对比表明,该集合模式能够较好地预报浒苔的位置、分布及其漂移路径。此外,该研究还讨论了Stokes漂流对于模式预报的影响,结果表明考虑Stokes漂流可以修正浒苔漂移路径,有效提高预报的准确性。

关键词：浒苔模式预报 Stokes漂流江苏近海

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