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The conditional mitochondrial protein complexome in the Arabidopsis thaliana root and shoot

Plant Communications 2023年第5期4卷 172-190页

作者： Youjun Zhang silvia martinez jaime Mustafa Bulut Alexander Graf Alisdair R.Fernie Center of Plant Systems Biology and Biotechnology 4000 PlovdivBulgaria Max-Planck-Institut f€ur Molekulare Pflanzenphysiologie Am Muhlenberg 114476 Potsdam-GolmGermany

Protein complexes are important for almost all biological processes.Hence,to fully understand how cells work,it is also necessary to characterize protein complexes and their dynamics in response to various cellular cues.Moreover,the dynamics of protein interaction play crucial roles in regulating the(dis)association of protein complexes and,in turn,regulating biological processes such as metabolism.Here,mitochondrial protein complexes were investigated by blue native PAGE and size-exclusion chromatography under conditions of oxidative stress in order to monitor their dynamic(dis)associations.Rearrangements of enzyme interactions and changes in protein complex abundance were observed in response to oxidative stress induced by menadione treatment.These included changes in enzymatic protein complexes involving g-amino butyric acid transaminase(GABA-T),D-ornithine aminotransferase(D-OAT),or proline dehydrogenase 1(POX1)that are expected to affect proline metabolism.Menadione treatment also affected interactions between several enzymes of the tricarboxylic acid(TCA)cycle and the abundance of complexes of the oxidative phosphorylation pathway.In addition,we compared the mitochondrial complexes of roots and shoots.Considerable differences between the two tissues were observed in the mitochondrial import/export apparatus,the formation of super-complexes in the oxidative phosphorylation pathway,and specific interactions between enzymes of the TCA cycle that we postulate may be related to the metabolic/energetic requirements of roots and shoots.

关键词： mitochondrial complexes BN-PAGE size-exclusion chromatography TCA cycle

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The Drying of Pyridine Hydrobromide

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Chinese Journal of Chemical Engineering 1998年第2期6卷 76-80页

作者： René D.PeraltaRodríguez Raúl G.LópezCampos jaime Wisniak Jacinto G.Rodríguez Gómez silvia Torres Rincón M.Esther TrevioMartínez Centro de Investigacion en Quimica Aplicada Blvd.Ing.Enrique Reyna Hermosillo No.140C.P.25100SaltilloCoahuilaMexico Department of Chemical EngineeringBen-Gurion University of the NegevBeer-ShevaIsraelCentro de Investigacion en Quimica AplicadaBlvd.Ing.Enrique Reyna Hermosillo No.140C.P.25100SaltilloCoahuilaMexico Department of Chemical EngineeringBen-Gurion University of the NegevBeer-ShevaIsraelCentro de Investigacion en Quimica AplicadaBlvd.Ing.Enrique Reyna Hermosillo No.140C.P.25100SaltilloCoahuilaMexico Department of Chemical EngineeringBen-Gurion University of the NegevBeer-ShevaIsraelCentro de Investigacion en Quimica AplicadaBlvd.Ing.Enrique Reyna Hermosillo No.140C.P.25100SaltilloCoahuilaMexico Department of Chemical EngineeringBen-Gurion University of the NegevBeer-ShevaIsraelCentro de Investigacion en Quimica AplicadaBlvd.Ing.Enrique Reyna Hermosillo No.140C.P.25100SaltilloCoahuilaMexico Department of Chemical EngineeringBen-Gurion University of the NegevBeer-ShevaIsraelCentro de Investigacion en Quimica AplicadaBlvd.Ing.Enrique Reyna Hermosillo No.140C.P.25100SaltilloCoahuilaMexico Department of Chemical EngineeringBen-Gurion University of the NegevBeer-ShevaIsrael

1 INTRODUCTIONThe drying of fine chemicals and pharmaceuticals is dominated by a number of special factorscompared to the drying of bulk chemicals.These include:very high value of product;lowthroughputs(typically,100 kg·h-1);materials that frequently possess toxic properties andare often sticky,pasty and difficult to handle when wet;the moisture to be evaporated ofteninvolves solvents;and multipurpose processing plants.

关键词： drying pyridine hydrobromide

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Cadaveric bone marrow mesenchymal stem cells:first experience treating a patient with large severe burns

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Burns & Trauma 2015年第2期3卷 74-82页

作者： Eduardo Mansilla Gustavo H.Marín Mirta Berges silvia Scafatti jaime Rivas Andrea Núñez Martin Menvielle Roberto Lamonega Cecilia Gardiner Hugo Drago Flavio Sturla Mercedes Portas silvia Bossi Maria Victoria Castuma Sandra Peña Luengas Gustavo Roque Karina Martire Jose Maria Tau Gabriel Orlandi Adrian Tarditti Tissue Engineering Regenerative Medicine and Cell Therapies LaboratoryCUCAIBAProvince of Buenos Aires Ministry of HealthTransplantation ProgramLa PlataProvince of Buenos AiresArgentina Burns and Plastic Surgery Department San Martín HospitalProvince of Buenos Aires Ministry of HealthLa PlataProvince of Buenos AiresArgentina Burns Hospital Buenos Aires CityArgentina Department of Chemistry Mayaguez CampusUniversity of Puerto RicoMayaguezPuerto RicoUSA.

Background:In January 2005,Rasulov et al.originally published"First experience in the use of bone marrow mesenchymal stem cells(MSCs)for the treatment of a patient with deep skin burns".Here,we present the first ever treated patient with cadaveric bone marrow mesenchymal stem cells(CMSCs)in the history of Medicine.Methods:A young man,who severely burned 60%of his total body surface with 30%of full-thickness burns while working with a grass trimmer that exploded,was involved in the study.MSCs were obtained from the bone marrow of a cadaver donor in a routine procurement procedure of CUCAIBA,the Province of Buenos Aires,Argentina,Ministry of Health,Transplantation Agency,cultured,expanded,and applied on the burned surfaces using a fibrin spray after early escharotomy.Results:So far,our preliminary experience and our early results have been very impressive showing an outstanding safety data as well as some impressive good results in the use of CMSCs.Conclusions:Based on al this,we think that improvements in the use of stem cells for burns might be possible in the near future and a lot of time as well as many lives could be saved by many other research teams all over the world.CMSCs will probably be a real scientific opportunity in Regenerative Medicine as well as in Transplantation.

关键词： Burns Cadaveric Transplantation Skin regeneration Mesenchymal stem cells

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Production of Volatile Moth Sex Pheromones in Transgenic Nicotiana benthamiana Plants

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BioDesign Research 2021年第1期2021卷 79-95页

作者： Rubén Mateos-Fernández Elena Moreno-Giménez silvia Gianoglio Alfredo Quijano-Rubio Jose Gavaldá-García Lucía Estellés Alba Rubert JoséLuis Rambla Marta Vazquez-Vilar Estefanía Huet Asunción Fernández-del-Carmen Ana Espinosa-Ruiz Mojca Juteršek Sandra Vacas Ismael Navarro Vicente Navarro-Llopis jaime Primo Diego Orzáez Institute for Plant Molecular and Cell Biology(IBMCP) Consejo Superior de Investigaciones Científicas(CSIC)-Universidad Politécnica de Valencia(UPV)ValenciaSpain Jaume I University Castellon de la PlanaSpain Department of Biotechnology and Systems Biology National Institute of BiologyLjubljanaSlovenia Jožef Stefan International Postgraduate School LjubljanaSlovenia Centro de Ecología Química Agrícola Instituto Agroforestal del MediterráneoUniversitat Politècnica de ValènciaValenciaSpain Ecología Y Protección Agrícola SL ValenciaSpain

Plant-based bioproduction of insect sex pheromones has been proposed as an innovative strategy to increase the sustainability of pest control in agriculture.Here,we describe the engineering of transgenic plants producing(Z)-11-hexadecenol(Z11-16OH)and(Z)-11-hexadecenyl acetate(Z11-16OAc),two main volatile components in many Lepidoptera sex pheromone blends.We assembled multigene DNA constructs encoding the pheromone biosynthetic pathway and stably transformed them into Nicotiana benthamiana plants.The constructs contained the Amyelois transitella AtrΔ11 desaturase gene,the Helicoverpa armigera fatty acyl reductase HarFAR gene,and the Euonymus alatus diacylglycerol acetyltransferase EaDAct gene in different configurations.All the pheromone-producing plants showed dwarf phenotypes,the severity of which correlated with pheromone levels.All but one of the recovered lines produced high levels of Z11-16OH,but very low levels of Z11-16OAc,probably as a result of recurrent truncations at the level of the EaDAct gene.Only one plant line(SxPv1.2)was recovered that harboured an intact pheromone pathway and which produced moderate levels of Z11-16OAc(11.8μg g^(-1) FW)and high levels of Z11-16OH(111.4μg g^(-1)).Z11-16OAc production was accompanied in SxPv1.2 by a partial recovery of the dwarf phenotype.SxPv1.2 was used to estimate the rates of volatile pheromone release,which resulted in 8.48 ng g-1 FW per day for Z11-16OH and 9.44 ng g^(-1 )FW per day for Z11-16OAc.Our results suggest that pheromone release acts as a limiting factor in pheromone biodispenser strategies and establish a roadmap for biotechnological improvements.

关键词： release dwarf transformed

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