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CXCR4+ and SDF-1+ Bone Marrow Cells Are Mobilized into the Blood Stream in Acute Myocardial Infarction and Acute Ischemia

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World Journal of Cardiovascular Diseases 2014年第7期4卷 361-367页

作者： Jose Luis Aceves Rafael Vilchis Maria Antonieta Medina Monserrat Borja silvia cortes Guillermo Diaz Armando Castro Alexis Gomez Jose JParra Martha Alvarado Manuel Lopez Hernandez Virna Poveda Felipe Masso Luis FMontano Division de Cardiocirugia Centro Medico Nacional 20 de Noviembre ISSSTEMexico DFMexico Departamento de Hematologia Centro Medico Nacional 20 de Noviembre ISSSTEMexico DFMexico Laboratorio de Hormonas Centro Medico Nacional 20 de Noviembre ISSSTEMexico DFMexico Departamento de Inmunobiologia Biologia Celular y Tisular Facultad de Medicina UNAMMexico DFMexico

Cell therapy has shown beneficial effects on ventricular function and tissue regeneration in patients with acute and chronic myocardial infarction, although with diverse grades of variability in the results, possibly by proportion, subtype and cell cycle status. Objective: Identify and phenotypically characterize, via CXCR4 and SDF-1 expression, the bone marrow cell subpopulations that are mobilized into the bloodstream in patients with Acute Myocardial Infarction (AMI) and Acute Ischemia (AI) such as acute angina and Chronic Ischemia (CI) such as chronic stable angina, and also determine the cell cycle status of these cells. Method: Patients with AMI and AI were recruited in the ICCU, and patients with CI in the departments of cardiology and cardiovascular surgery. The quantification of cellular subpopulations was made by cytofluorometry with a FACS caliburcyto fluorometry (Becton Dickinson) with specific FITC-labeled anti human monoclonal antibodies against CD34, CD133, CD117, CD48, CXCR4, SDF-1 and Ki67 (Becton Dickinson). Serum concentration of IL-6 and IL-8 were determined by a sequential solid phase chemiluminescent assay performed in a SIEMENS IMMULITE 1000 Analyzer. Statistical analysis was made with the SPSS version 20.0 for Windows. A p value 3/ml) than that in AI (9.2 ± 1.3 × 103/ml) and CI (6.6 ± 1.1 × 103/ml) patients (p p = 0.22 to 0.39), but interestingly in AMI and AI patients, cells were CXCR4+ in almost half of these mobilized cells, although the proportion was significantly higher in AMI patients (46.8% ± 7.1% to 55.7% ± 6.3% vs 23% ± 1.6% to 28.4% ± 2.1%, p = 0.03 to 0.05). A similar behavior was observed with the Ki67 antibody (29.9% ± 2.1% to 36.1% ± 6.3% vs 10% ± 1.2% to 24% ± 1.1%, p = 0.001 to 0.05). Bivariate analysis of the results showed a significant correlation of the cell proportion in AMI but not in AI and CI patients (p = 0.001 to 0.05;0.12 to 0.87 and 0.17 to 0.92 respectively). The amount of myocardial tissue infarcted did not show

关键词： Stem Cells CXCR4:SDF-1 Axis Cardiac Repair Acute and Chronic Ischemia

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伴有LADD综合征的角膜缘干细胞缺乏

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美国医学会眼科杂志（中文版） 2006年第1期18卷 30-32页

作者： Magdalena cortes Alessandro Lambiase Marta Sacchetti silvia Aronni Stefano Bonini 陈旭（译）朱志忠（校）

泪管-耳-牙-指（趾）（LADD）综合征是一种具有多种表现的常染色体显性遗传疾病。最初在1967年由Levy报道。1例双侧泪液系统缺失、杯状耳、干嘴，以及牙、手臂和指（趾）异常的单发病例。之后一些新发现的临床表现，如肾脏异常、唾液腺... 详细信息

泪管-耳-牙-指（趾）（LADD）综合征是一种具有多种表现的常染色体显性遗传疾病。最初在1967年由Levy报道。1例双侧泪液系统缺失、杯状耳、干嘴，以及牙、手臂和指（趾）异常的单发病例。之后一些新发现的临床表现，如肾脏异常、唾液腺缺乏、先天性髋脱位、先天性裂孔疝和横膈疝、感觉性耳聋和传导性耳聋、牙发育不全、四肢异常、口腔干燥和眼干都被报道与此综合征有关。

关键词：角膜缘干细胞缺乏综合征常染色体显性遗传疾病先天性髋脱位牙发育不全传导性耳聋肾脏异常临床表现口腔干燥杯状耳

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Life Cycle Assessment of Hydrogel Used in Hydroponics for the Impatiens New Guinea Production

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Open Journal of Applied Sciences 2016年第5期6卷 310-314页

作者： silvia Paola Venegas Segura Martha Lilia Domínguez Patiño Miguel Aguilar cortes Jorge Avelino Domínguez Patiño Luz Elva Marín Vaca Nadia Lara Ruiz Rosa María Melgoza Alemán Oscar Gabriel Villegas Torres Facultad de Ciencias Químicas e Ingeniería Universidad Autónoma del Estado de Morelos Cuernavaca México Centro de Investigación en Ingeniería y Ciencias Aplicadas Universidad Autónoma del Estado de Morelos Cuernavaca México Facultad de Ciencias Agropecuarias Universidad Autónoma del Estado de Morelos Cuernavaca México

The environmental impact on the planet leads to the search of new processes that are friendly with the environment and obtaining high quality products. In this sense, green chemistry is used in the generation of products through processes that do not affect the planet. In many of these processes and in general, the use of water has been depleted this resource of vital importance for the survival of living things. In Mexico, 77% of water is used in agriculture;14% in the public supply;5% in the thermoelectric plants and 4% in the industry. In research presented, hydroponics is essential in the process and is defined as a technique used to reduce excessive water consumption, by providing the necessary nutrients, in addition to preventing soil erosion by allowing the growth of plants without use of ground. The potassium polyacrylate is a super-absorbent polymer (Hydrogel) capable of absorbing water up to 500 times of its weight and has a life span of 10 years. When mixed with a substrate, the required nutrient solution potassium polyacrylate allows to lengthen the interval watering and thus reducing the consumption of water from plants. Morelos is the largest producer of ornamental plants in the country, due to its privileged climate and location. In the present work using the hydroponic methodology, we planted cuttings of Bethlehem New Guinea (Impatiens New Guinea) in a substrate made by 60% of coconut fiber, 20% tepojal, 20% ground sheet mixed with polymer of potassium (20 g per litre), 400 ml of Steiner nutrient solution in concentrations of 40%, 60%, 80% and 100%, varying the interval of 3-, 6-, 8- and 10-day watering. The variables considered are: concentration of chlorophyll, the plant height, leaf area, number of stems and weight dry total. The 12 principles of green chemistry applied to hydroponics using polyacrylate of potassium as part of the substrate in Bethlehem New Guinea (Impatiens New Guinea) to determine the analysis of life cycle (LCA), doing the bala

关键词： Steiner Solution Environmental Impact SimaPro

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