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Fatty Acid Deposition on Broiler Meat in Chickens Supplemented with Tuna Oil

Food and Nutrition Sciences 2013年第9期4卷 16-20页

作者： Jesus Eduardo Morales-Barrera Mariano Jesus Gonzalez-Alcorta Rosa Maria Castillo-dominguez Omar Francisco Prado-Rebolledo Xochitl Hernandez-Velasco Anita Menconi Guillermo Tellez Billy Marshal Hargis silvia carrillo-dominguez Departamento de Producción Agrícola y Animal Universidad Autónoma Metropolitana México City México Department of Poultry Science University of Arkansas Fayetteville USA. Depto. Nutrición Animal Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán México City México Facultad de Medicina Veterinaria y Zootecnia Universidad de Colima Colima México Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Autónoma de México México City México Universidad Autónoma Chapingo Estado de México México

The aim of the present study was to evaluate the effect of feeding four levels of tuna oil on performance and fatty acid (FA) profiles of broiler chicken meat. 240 Ross broiler chickens were randomly assigned to 20 pens and divided into four treatments: 0%, 0.75%, 1%, and 1.25% of tuna oil. At 49 days, breast and legs-thighs muscles were processed for FA analysis. Concentrations of FA in legs and thighs meat were significantly higher when compared with breast meat. In both types of meats (breast and legs-thighs), the inclusion of 1.0% or 1.25% of tuna oil in the diet significantly increased (P < 0.05) the concentration of n-3 FA (especially docosahexaenoic and eicosapentaenoic acids). Fat in broiler breast contained a proportion of 29% saturated FA (SFA): 36% monosaturated FA (MFA): 35% polyunsaturated FA (PUFA);while legs and thighs meat had a proportion of 28% SFA: 38% MFA: 33% PUFA. The addition of tuna oil in the broiler diet significantly reduced the deposition of SFA, MFA, and PUFA in breast meat, while in legs and thighs these reductions were less noticeable. The significant reduction in the concentration of n-6 PUFA and the increment of n-3 PUFA were more evident in breast than in legs and thighs, and with the addition of 1% and 1.25% of tuna oil. The results of the present study suggest a difference in FA deposition attributable to supplementation with tuna oil. The addition of tuna oil could be recommendable to increase n-3 PUFA in both broiler chicken breast and legs-thighs meats, providing a healthier and functional chicken meat to consumer.

关键词： n-3 Fatty Acids Broilers Tuna Oil Breast Meat Legs and Thighs Meat

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Effect of Time and Fatty Acid Composition in Eggs of White Leghorn Hens Supplemented with Tuna Oil

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Food and Nutrition Sciences 2013年第9期4卷 39-44页

作者： Jesus Eduardo Morales-Barrera Mariano Jesus Gonzalez-Alcorta Rosa Maria Castillo-dominguez Omar Francisco Prado-Rebolledo Jose Luis Vazquez Xochitl Hernandez-Velasco Guillermo Tellez Anita Menconi Billy Marshal Hargis silvia carrillo-dominguez Departamento de Producción Agrícola y Animal Universidad Autónoma Metropolitana México City México Department of Poultry Science University of Arkansas Fayetteville USA. Depto. Nutrición Animal Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán México City México Facultad de Medicina Veterinaria y Zootecnia Universidad de Colima Colima México Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Autónoma de México México City México Universidad Autónoma Chapingo Estado de México México

The study evaluated the effect of time and fatty acid (FA) composition in eggs of laying hens supplemented with tuna oil (TO). Two hundred White Leghorn hens 30-week were divided into 5 treatments with 4 replicates of 10 birds each. Treatments were assigned randomly and consisted of 0%, 0.75%, 1.50%, 2.25% and 3% TO in commercial sorghum-soybean meal diets, in which the soybean oil was partially replaced. The experiment was conducted for 90 days. At days 28, 56 and 84 of the experiment, 10 eggs per replicate were collected for chemical analysis. Feed intake, egg production rate, egg weight, and feed conversion were not influenced by dietary treatment. FA content was significantly altered (P < 0.05) by TO, showing a progressive increase in egg n-3 FA (especially docosahexaenoic [DHA] and eicosapentaenoic [EPA] acids) when TO was added. Levels of EPA and DHA were higher (P < 0.05) in the egg lipids of TO fed hens than those in the control group. This was correlated (P < 0.05) with the concentration of TO in the diet. However, no significant differences were observed either in egg weight or percent of egg lay between groups. There was a high correlation (P < 0.05) between TO inclusion level in the diet and the incorporation of EPA (r = 0.96) or DHA (r = 0.92) into the egg yolk and an overall decrease in n-6 FA. There was a high correlation (P < 0.05) between TO inclusion level and the incorporation of linoleic acid (LA, r = -0.95) or arachidonic acid (AA, r = -0.96) into the egg yolk. The highest incorporation (P < 0.05) of total n-3 FA content in eggs was obtained with 3% TO/kg. This increase was proportional to TO inclusion levels in the diets (r = 0.95). The results indicate that the n-3 FA content in eggs can be increased by dietary supplementation with TO, and that TO can serve as a reasonable alternative feed ingredient in layer diets to produce a healthier choice of egg.

关键词： Egg Enrichment Laying Hens n-3 Fatty Acids Tuna Oil

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The complex role of protocadherin-19 in brain function:a focus on the oxytocin system

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Neural Regeneration Research 2025年第11期20卷 3211-3212页

作者： Sara Mazzoleni Marta Busnelli silvia Bassani CNR Institute of Neuroscience Vedano al LambroItaly NeuroMi Milan Center for Neuroscience MilanItaly

Mutations in the protocadherin-19(PCDH19)gene(Xq22.1)cause the X-linked syndrome known as developmental and epileptic encephalopathy 9(DEE9,OMIM#300088)(Dibbens et al.,2008).DEE9 is characterized by early-onset clustering epilepsy associated with intellectual disability ranging from mild to profound,autism spectrum disorder,and other neuropsychiatric features including schizophrenia,anxiety,attentiondeficit/hyperactivity,and obsessive or aggressive *** seizures may become less frequent in adolescence,psychiatric comorbidities persist and often worsen with age(Dibbens et al.,2008;Kolc et al.,2020).

关键词： epilepsy function system

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How Plants Cope with Cadmium: Staking All on Metabolism and Gene Expression

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Journal of Integrative Plant Biology 2008年第10期50卷 1268-1280页

作者： Giovanni DalCorso silvia Farinati silvia Maistri Antonella Furini Dipartimento Scientifico e Tecnologico Universita` degli Studi di Verona Strada Le Grazie 15 37134 Verona Italy

Environmental pollution is one of the major problems for human health. Toxic heavy metals are normally present as soil constituents or can also be spread out in the environment by human activity and agricultural techniques. Soil contamination by heavy metals as cadmium, highlights two main aspects： on one side they interfere with the life cycle of plants and therefore reduce crop yields, and on the other hand, once adsorbed and accumulated into the plant tissues, they enter the food chain poisoning animals and humans. Considering this point of view, understanding the mechanism by which plants handle heavy metal exposure, in particular cadmium stress, is a primary goal of plant-biotechnology research or plant breeders whose aim is to create plants that are able to recover high amounts of heavy metals, which can be used for phytoremediation, or identify crop varieties that do not accumulate toxic metal in grains or fruits. In this review we focus on the main symptoms of cadmium toxicity both on root apparatus and shoots. We elucidate the mechanisms that plants activate to prevent absorption or to detoxify toxic metal ions, such as synthesis of phytochelatins, metallothioneins and enzymes involved in stress response. Finally we consider new plant-biotechnology applications that can be applied for phytoremediation.

关键词： cadmium heavy metals metallothioneins phytochelatins phytoremediation transporters.

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Decoding Phthorimaea operculella(Lepidoptera: Gelechiidae) in the new age of change

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Journal of Integrative Agriculture 2020年第2期19卷 316-324页

作者： silvia I.RONDON Hermiston Agricultural Research and Extension Center Crop and Soil ScienceOregon State University

Arthropods and pathogens constantly challenge potato Solanum tuberosum *** of climate variation have increased the possibility of changes in pests’biological and ecological patterns by increasing or reducing overwintering length,changes in population growth rates,number of generations,crop-pest relationship,and therefore affecting their *** operculella Zeller(Lepidoptera:Gelechiidae)is one of the main pests affecting potatoes *** oviposit single or multiple eggs in leaves,stems,and tubers,while the larvae in immature stage mine leaves or burrows into tubers turning them *** control methods are effective in controlling ***,but many factors determine the success of the control *** review provides key highlights of current information available that could be used as a resource to fight this pest.

关键词： climate insects moth potato pest management Solanaceae pest control IPM tubermoth potato tuber moth

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Genotoxicity biomarkers in aquatic bioindicators

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Current Zoology 2014年第2期60卷 273-284页

作者： Claudia BOLOGNESI silvia CIRILLO Environmental Carcinogenesis Unit IRCCS Azienda Ospedaliera Universitaria San Martino- IST Istituto Nazionale Ricerca sulCancro L.go Rosanna Benzi 10 Genova Italy

Pollution of the aquatic environment is an ever-growing problem, as waters are the ultimate sink for the large number of xenobiotics from multiple sources. DNA damaging agents have a significant ecological relevance since they are implicated in many pathological processes and exert effects beyond that of individual being active through following generations. A large number of methods have been applied to evaluate genotoxic damage in different aquatic species. Comet assay, as method for de- tecting DNA alterations, and micronucleus test, as an index of chromosomal damage are the most widely applied and validated methods in field studies. These methods were applied in different vertebrate and invertebrate aquatic species, but only mollusk and fish species have been employed in routine biomonitoring programs. Mussels, due to their widely geographical distribution and the suitability for caging represent the bioindicator of choice in field studies. Mytilus species is the most used marine mussel. The use of fish is limited to specific geographic areas. The present review mainly focuses on the application of comet assay and micronucleus test in mussels. A number of biomonitoring studies in mussels, using comet assay or micronucleus test, revealed exposure to different classes of genotoxic compounds with a good discrimination power. The different evidence from the two as- says, reflects different biological mechanisms for the two genetic endpoints, DNA damage and chromosomal damage, suggesting their combined application in the field. Different endogenous and exogenous factors have been shown to modulate the genotoxic responses in mussels, acting as confounding factors in environmental monitoring. The use of standardized protocol for caging, sampling and genotoxity evaluation is critical in biomonitoring studies. The use of a multimarker approach coupling genotoxicity biomarkers with physiological and biochemical factors allows to have a complete picture of the environmenta

关键词： Aquatic animals Genotoxicity Bioindicator Biomarker Micronucleus test Comet assay Bivalve Mussel

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PCDH19 interplay with GABA(A)receptors:a window to DEE9 pathogenetic mechanisms

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Neural Regeneration Research 2022年第4期17卷 803-805页

作者： Sara Mazzoleni silvia Bassani Institute of Neuroscience CNRMilanItaly Department of Medical Biotechnology and Translational Medicine University of MilanSegrate(MI)Italy NeuroMI Milan Center for Neuroscience University of Milano-BicoccaMilanItaly

Developmental and epileptic encephalopathy 9(DEE9):The gene PCDH19(Xq22.1),which encodes the calcium-dependent cell adhesion protein protocadherin-19(PCDH19),is nowadays considered as one of the most important genes in monogenic epilepsy(Depienne and LeGuern,2012).Mutations in PCDH19 are responsible for DEE9(OMIM#300088),a severe neurodevelopmental disorder characterized by early-onset clustering epilepsy,various degrees of cognitive impairment and neuro psychiatric comorbidities,like autism spectrum disorder(ASD)and behavioural ***9 patients start suffering from seizures around the age of 10 months until adolescence,when seizures tend to reduce or even disappear,while the psychiatric symptoms persist(Depienne and LeGuern,2012;Kolc et al.,2018).

关键词： H19 PCD epilepsy

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Sperm priming response to perceived mating opportunities is reduced in male guppies with high baseline sperm production

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Current Zoology 2018年第2期64卷 205-211页

作者： silvia CATTELAN Andrea PILASTRO Department of Biology University of Padova Padova 35131 Italy

Producing sperm is costly and males have been selected to strategically adjust their sperm produc- tion and/or expenditure according to the fitness return associated with a specific mating. For exam- ple, males respond to fluctuations in the mating opportunities by adjusting the number of ＂ready＂ sperm. This phenomenon is known as ＂sperm priming＂ and is interpreted as a strategy to econo- mize the investment in sperm. The cost and benefits of the sperm priming response, however, are expected to depend on a male＇s baseline sperm production （BSP） in the absence of females, because of the different risk of sperm depletion and the nonlinearly increasing costs of sperm pro- duction. We tested this prediction in 2 replicated lines of male guppies Poecilia reticulata that were artificially selected for high and low BSP. BSP has a large genetic variance and a high sire heritabil- ity in guppies, and males respond to the perceived mating opportunities by increasing the number of ＂ready＂ sperm. We investigated whether males with a different BSP differed in their sperm pri- ming response. We found that when the perceived mating opportunities increased, males from low-sperm lines had a stronger sperm priming response than those from high-sperm lines. This result suggests that adaptive plasticity in sperm priming has the potential to evolve in response to different levels of BSP. The comparison between guppy populations with different levels of sperm production would allow to test whether the pattern reported here is also observed at the interpopulation level.

关键词： artificial selection mate availability Poecilia reticulata sperm investment sperm priming sperm production.

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Seropositivity for Trypanosoma cruzi in domestic dogs from Sonora,Mexico

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Infectious Diseases of Poverty 2017年第1期6卷 1067-1073页

作者： Minerva Arce-Fonseca silvia C.carrillo-Sanchez Ramon M.Molina-Barrios Mariana Martinez-Cruz Jesus R.Cedillo-Cobian Yuly A.Henao-Diaz Olivia Rodriguez-Morales Department of Molecular Biology National Institute of Cardiology“Ignacio Chavez”Juan Badiano No.1Col.Seccion XVIDelegacion Tlalpan14080 Mexico CityMexico Department of Agronomics and Veterinary Sciences Technological Institute of Sonora5 de Febrero 818 SurCentro85000Cd ObregonMexico CitySonoraMexico

Background:Chagas disease is an important health problem in Latin America due to its incapacitating effects and associated *** on seropositivity for Trypanosoma cruzi in Mexican dogs have demonstrated a direct correlation between seropositivity in humans and dogs,which can act as sentinels for the disease in this *** objective of this study was to determine the seropositivity for *** infection in dogs from Sonora,a northern borderstate of ***:Responsible pet owners were selected at random from an urban area of Empalme municipality,Sonora,Mexico,and from there,180 dog samples were *** antibodies were determined using the enzymelinked immunosorbent assay(ELISA)*** ELISA sera were processed by indirect immunofluorescence to confirm the presence of *** *** the statistical analysis,chi-square tests were ***:Dogs’sera showed a seropositivity rate of 4.44%.The rate of seropositivity was not associated with the dogs’age,sex,or socioeconomics pertaining to the geographical *** sample(1/180,0.55%)showed the acute state of the ***:The study found a presence of *** antibodies in dogs in this area,which suggests vector *** is a need for active surveillance programs throughout the state of Sonora and vector control strategies should also be implemented in endemic regions.

关键词： Chagas disease Epidemiology Reservoir US-Mexico border dogs Trypanosoma cruzi Mexico

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Potential therapeutic effects of polyphenols in Parkinson's disease:in vivo and in vitro pre-clinical studies

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Neural Regeneration Research 2021年第2期16卷 234-241页

作者： Claudio Giuliano silvia Cerri Fabio Blandini Laboratory of Cellular and Molecular Neurobiology IRCCS Mondino FoundationPaviaItaly

Parkinson's disease is a neurodegenerative disorder characterized by a combination of severe motor and non-motor *** the years,several factors have been discovered to play a role in the pathogenesis of this disease,in particular,neuroinflammation and oxidative *** date,the pharmacological treatments used in Parkinson's disease are exclusively *** this reason,in recent years,the research has been directed towards the discovery and study of new natural molecules to develop potential neuroprotective therapies against Parkinson's *** this context,natural polyphenols have raised much attention for their important anti-inflammatory and antioxidant properties,but also for their ability to modulate protein *** this review,we propose to summarize the relevant in vivo and in vitro studies concerning the potential therapeutic role of natural polyphenols in Parkinson's disease.

关键词： alpha-synuclein anti-inflammatory antioxidants natural molecules neuroprotection Parkinson's disease polyphenols syntomatic effect

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