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Expression and activity of heterologous hydroxyisocaproate dehydrogenases in Synechocystis *** 6803ΔhoxYH

Engineering Microbiology 2022年第1期2卷 1-7页

作者： Valentina Jurka Christoph K.Winkler silvan poschenrieder Paulo Oliveira Catarina C.Pacheco Eunice A.Ferreira Florian Weissensteiner Piera De Santis Selin Kara Robert Kourist Paula Tamagnini Wolfgang Kroutil a Institute of Chemistry University of GrazNAWI GrazHeinrichstrasse 288010 GrazAustria i3S–Instituto de Investigação e Inovação em Saúde Universidade do Porto4200-135 PortoPortugal IBMC–Instituto de Biologia Molecular e Celular Universidade do Porto4200-135 PortoPortugal Departamento de Biologia Faculdade de CiênciasUniversidade do Porto4169-007 PortoPortugal ICBAS–Instituto de Ciências Biomédicas Abel Salazar Universidade do Porto4050-313 PortoPortugal Aarhus University Department of EngineeringBiological and Chemical Engineering SectionBiocatalysis and Bioprocessing GroupGustav Wieds Vej 10DK 8000 AarhusDenmark Institute of Molecular Biotechnology Graz University of Technology8010 GrazAustria Field of Excellence BioHealth University of Graz8010 GrazAustria BioTechMed Graz 8010 GrazAustria.

Exploiting light to drive redox reactions is currently a hot topic since light is considered as an environmentally friendly source of ***,cyanobacteria,which can use light e.g.,for generating NADPH,are in the focus of ***,it has been shown that various heterologous redox enzymes could be expressed in these *** we demonstrated the successful inducer-free expression of𝛼-keto-acid dehydroge-nases(L-HicDH and D-HicDH)from Lactobacillus confusus DSM 20196 and Lactobacillus paracasei DSM 20008 in Synechocystis *** 6803ΔhoxYH mutant using replicative *** the L-HicDH showed poor activity limited by the amount of expressed enzyme,the D-HicDH was applied both in vivo and in vitro,transforming the selected𝛼-keto acids to the corresponding optically pure(R)-𝛼-hydroxy acids(ee>99%)in up to 53%and 90%conversion,respectively.

关键词： Cyanobacteria Biocatalysis Photobiotransformation Synechocystis sp.PCC 6803 Hydroxyisocaproate dehydrogenase Ketoacid dehydrogenase

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Forestry projections for species diversity-oriented management:an example from Central Europe

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Ecological Processes 2018年第1期7卷 244-261页

作者： Astor Toraño Caicoya Peter Biber Werner poschenrieder Fabian Schwaiger Hans Pretzsch Chair of Forest Growth and Yield Science Center of Life and Food Sciences WeihenstephanTechnische Universität MünchenHans-Carl-von-Carlowitz 2D-85354 FreisingGermany

Introduction:Changes in socio-economy and climate are affecting the demand of wood products *** the same time,society requires that forest supporting structures like biodiversity are maintained and preserved while the demand for wood products is also *** support systems,like forest simulation models,that are able to analyze connections as well as quantify trade-offs between forest structure management and biodiversity indicators are highly ***,such models are generally developed for the local plot or stand scale only and ecosystem-scale analyses are *** this study,we analyzed ways to interpret results from the single-tree forest simulator SILVA from the local to the ecosystem *** also analyzed the impacts of forest management on biodiversity using two species diversity indicators,the species profile index and the species intermingling,for scenarios adapted from the GLOBIOM model in the case study“Augsburg Western Forests”,a high productive region in *** order to evaluate diversity tendencies across the ecosystem,we applied a moving window ***:The relevance of scale for the interpretation of management effects on species diversity was shown and clear differences between scenarios *** differences between scenarios were particularly visible when comparing the two diversity indicators,especially because the species profile index focuses on vertical and horizontal information and the species intermingling focuses mainly on horizontal *** a multifunctional scenario,biodiversity values could be preserved at all scales in the vertical ***,under a bio-energy-oriented scenario diversity at the local scale was reduced,although at the ecosystem level,and only in the horizontal dimension,diversity remained at relatively high ***:With this work,we can conclude that integrative modeling,with multiple scenarios,is highly needed to support forestry decision m

关键词： Forest model Multifunctional forestry Growing window GLOBIOM

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Current Soil Nutrient Status of Intensively Managed Greenhouses

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Pedosphere 2012年第6期22卷 825-833页

作者： HU Yun-Cai SONG Zhi-Wen LU Wen-Long C. poschenrieder U. SCHMIDHALTER Chair of Plant Nutrition Technische Universitat Miinchen D-85350 Freising (Germany) Institute of Information Tianjin Academy of Agricultural Sciences Tianjin (China)

Nine districts covering the main greenhouse vegetable areas in Tianjin Municipality of the North China Plain were selected for the soil investigation in 2010 to survey the current soil nutrient status (soil available N, P and K), acidification and salinization due to excessive input of fertilizers in greenhouses in Tianjin. The study showed that, in particular, soil available P content increased with the age of greenhouses. In contrast, our results did not reveal higher K accumulation and lowered pH in the greenhouse soils compared with cultivation in open fields. Over-fertilization, causing high NO 3 accumulation, most likely resulted in salinity problems in the greenhouses. Ninety percent of the investigated greenhouse soils had electrical conductivity values of saturated paste extracts of 2-10 d S m-1 , which might affect the yields of vegetable crops like green bean, pepper, cabbage, carrot, eggplant, lettuce, spinach, celery, cucumber and tomato. The findings of our survey of the current fertility and salinity problems in greenhouse soils suggest that there is an urgent need to improve the farmers' practices and strategies in fertilization management in greenhouses of China. Because education and the agricultural technical extension services may play a more important role in avoiding overuse of fertilizers, we suggest that current nutrient management practices should be improved in the near future through training of local farmers in farmers' schools and through strengthening the agricultural extension services with practical techniques.

关键词： electrical conductivity greenhouse vegetables over-fertilization soil pH soil survey

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High Quality DNA Obtained from a Single Seed of <i>Vitis vinifera</i>L. Using Rapid DNA Extraction Method

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American Journal of Plant Sciences 2014年第13期5卷 2023-2030页

作者： Ajith Samantha Rathnayake Josep Allué Mercè Llugany Anna Puig-Pujol Kshanika Hirimburegama Charlotte poschenrieder Department of Agriculture Research and Technology Food and Agriculture Institute (IRTA)—Catalan Institute of Vine and Wine (INCAVI) Vilafranca del Penedés Spain Department of Plant Sciences Faculty of Science University of Colombo Colombo Sri Lanka Plant Physiology Laboratory Faculty of Biosciences Autonomous University of Barcelona (UAB) Bellaterra Spain Plant Physiology Laboratory Faculty of Biosciences Autonomous University of Barcelona (UAB) Bellaterra Spain Department of Plant Sciences Faculty of Science University of Colombo Colombo Sri Lanka

Use of a single seed is very useful for genetic studies on Vitis vinifera. However, molecular markers require a fair amount of high purity DNA. Grapevine contains high concentrations of polysaccharides, polyphenols, tannins and other secondary metabolites. These compounds may hamper the DNA isolation processes and subsequent analysis. In this study we have compared two DNA isolation methods: the NucleoSpin Plant II method and a modified protocol from Doyle and Doyle. The average value of 260/280 nm absorption ratio, which is used to assess the purity of DNA and RNA was 1.8 (accepted as “pure” DNA) and 0.9 (presence of protein or other contaminants) for the first and second method, respectively. Using the NucleoSpin protocol, from a single seed (20 - 35 mg) we obtained an average yield of extracted DNA of 24.8 ± 5.2 to 38.4 ± 11.5 ng·mg-1 dry weight. Among the two protocols examined, the NucleoSpin method was more efficient and gave better quality of DNA values compared to those from the modified Doyle and Doyle procedures.

关键词： Vitis Single Seed High Quality DNA DNA Extraction Method

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Comparison of high-resolution ultrasound and MR-enterography in patients with inflammatory bowel disease

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World Journal of Gastroenterology 2011年第8期17卷 1018-1025页

作者： Andreas G Schreyer Cynthia Menzel Chris Friedrich Florian poschenrieder Lukas Egger Christian Dornia Gabriela Schill Lena M Dendl Doris Schacherer Christl Girlich Ernst-Michael Jung Ernst-Michael Jung Institute of Radiology University Medical Center Ernst-Michael Jung De-partment of Interdisciplinary Ultrasound University Medical Center

AIM:To compare the results of high-resolution ultrasound(HR-US) and magnetic resonance enterography(MRE) examinations in patients with inflammatory bowel disease(IBD).METHODS:The reports of 250 consecutive cases with known IBD,who had an MRE and HR-US examination,were retrospectively *** a patient-based approach we evaluated morphological disease features such as affected bowel wall,stenosis,abscess and *** comparison between the two modalities was based on the hypothesis,that any pathological change described in any imaging modality was a true finding,as no further standard of reference was available for complete ***:Two hundred and fifty examinations representing 207 different patients were *** modalities assessed similar bowel wall changes in 65% of the examinations,with more US findings in 11% and more MRE findings in 15%.When the reports were analyzed with regard to "bowel wall inflammation",US reported more findings in 2%,while MRE reported more findings in 53%.Stenoses were assessed to be identical in 8%,while US found more in 3% and MRE in 29%(P < 0.01).For abscess detection,US showed more findings in 2%(n = 4) while MRE detected more in 6%(n = 16).US detected more fistulas in 1%(n = 2),while MRE detected more in 13%(n = 32)(P < 0.001).The most common reason for no detected pathology by US was a difficult to assess anatomical region(lesser pelvis,n = 72).CONCLUSION:US can miss clinically relevant pathological changes in patients with IBD mostly due to difficulty in assessing certain anatomical regions.

关键词： Crohn’s disease Diagnosis Inflammatory bowel disease Magnetic resonance imaging Ultrasound

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天津市设施蔬菜土壤硝态氮状况研究

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天津农业科学 2012年第2期18卷 91-94页

作者：程文娟潘洁吕雄杰赵先胜 Christoph poschenrieder 胡云才 Urs Schmidhalter 天津市农业资源与环境研究所天津300192 天津市农业科学院信息研究所天津300192 慕尼黑工业大学德国慕尼黑81245

以天津市主要蔬菜产区为研究对象,通过分析大棚内菜地耕层土壤及0～90cm垂直分层土样,研究蔬菜地氮肥污染现状。结果表明,耕层土壤硝态氮积累量表现为蓟县>西青>静海>北辰,且菜地土壤硝态氮含量随土层深度的增加有递减趋势;蔬菜地土壤... 详细信息

以天津市主要蔬菜产区为研究对象,通过分析大棚内菜地耕层土壤及0～90cm垂直分层土样,研究蔬菜地氮肥污染现状。结果表明,耕层土壤硝态氮积累量表现为蓟县>西青>静海>北辰,且菜地土壤硝态氮含量随土层深度的增加有递减趋势;蔬菜地土壤盐分与土壤硝态氮含量之间呈极显著的正相关;手持田间快速硝态氮测定仪对农田测土施肥具有一定的指导作用。

关键词：天津土壤硝态氮蔬菜大棚

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