检索结果-南通市图书馆

Panhandle PCR strategy to amplify the upstream unknown sequence of the Pr1 gene of pythium guiyangense

Journal of Medical Colleges of PLA(China) 2013年第5期28卷 268-272页

作者： duan siliang SU Xiaoqing YU Sheng Medical College Guangxi University of Science and Technology Department of Biology Guiyang Medical College

Objective： Based on a partialsubtilisin-like protease, Prl genomic sequence ofPythium guiyangense which has been cloned before, Panhandle PCR strategy was used to amplify the upstream flanking sequence adjacent to the known sequence of the Prl gene. Methods： The genomic DNA was firstly digested with BamH I and then treated with calf intestinal alkaline phosphatase（CIAP）. Next, a 5＇ phosphorylated oligonucleotide was ligated to the 5＇ ends of BamH I -digested DNA. After denaturation, intmstrand annealing and polymemse extension, a pan with a handle was formed,and lastly the nested PCR was performed. Results： A 864 bp product was amplified,which was adjacent to the known sequence of Prl *** gene has been accessed by GenBank （Accession：JQ975036）. Conclusion： Panhandle PCR is a quick and convenient approach for amplifying and identifying unknown partner genes,which facilitates cloning full-length Prl gene

关键词： Panhandle PCR Upstream regulation sequence Subtilisin-like protease Prl Chromosome walking

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enhanced anti-tumor immunity against breast cancer induced by whole tumor cell vaccines genetically modified expressing α-Gal epitopes

Enhanced anti-tumor immunity against breast cancer induced b...

引用

第十二届全国免疫学学术大会

作者： Xue Dabing Liang Ying duan siliang He Jian Su Jing Zhu Jianmeng Hu Nan Liu Jianming Zhao Yongxiang Lu Xiaoling National Center for International Research of Biological Targeting Diagnosis and Therapy Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy ResearchCollaborative Innovation Center for Targeting Tumor Diagnosis and TherapyGuangxi Medical University Department of Respiratoy Diseases The Third Hospital of Central South University The Department of Immunology Guangxi Medical University

Whole tumor cell vaccines have shown much promise,but were demonstrated poor efficiency in phase Ⅲ *** this study,we modified MDA-MB-231 tumor cells(MDA-MB-231 Gal+) to express a-1,3-galactosyltransferase(α-1,3-GT) protein,to potentially enhance antitumor effect of whole tumor cell ***-MB-231 tumor cell vaccines were transfected with a reconstructed lentiviral containing α-1,3-GT *** growth,tumorigenesis and survival of Hu-NODSCID mice were observed when tumor-bearing mice were injected with tumor cell *** and apoptosis in MDA-MB-231 tumor xenografts were observed by *** levels of cytokine secretion in the serum of mice were tested by ***8+ T cells infiltrating tumors were assessed by flow ***-MB-231 Gal+ cells expressed active α-1,3-GT and produced a-Gal in ***-MB-231 Gal+ cell vaccines suppressed tumor growth and tumorigenesis in immunized Hu-NOD-SCID ***,decrease of TGF-β,IL-10 and increase of INF-γ,IL-12 were observed in tumor cell vaccinated ***,the cell vaccines enhanced infiltration of cytotoxic CD8+ T cells in the tumormicroenvironment of immunized *** MDA-MB-231 Gal+ cell vaccines modified α-1,3-GT genes improved the antitumor effect.

关键词： whole tumor cell vaccines α-1 3-GT antitumor

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Mouse IP-10 Gene Delivered by Folate-modified Chitosan Nanoparticles and Dendritic/tumor Cells Fusion Vaccine Effectively Inhibit the Growth of Hepatocellular Carcinoma in Mice

Mouse IP-10 Gene Delivered by Folate-modified Chitosan Nanop...

引用

第十二届全国免疫学学术大会

作者： Hu Ziqian Chen Jiaojiao Zhou Sufang Yang Nuo duan siliang Zhang Zhenghua Su Jing He Jian Zhang Zhiyong Lu Xiaoling Zhao Yongxiang National Center for International Research of Biological Targeting Diagnosis and Therapy Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy ResearchCollaborative Innovation Center for Targeting Tumor Diagnosis and TherapyGuangxi Medical University Department of Surgery Robert-Wood-Johnson Medical School University HospitalRutgers University

Dendritic cells(DC) and tumor cell fusion vaccine(DC/tumor cell fusion vaccine) is considered an effective approach in cancer ***,its therapeutic effects in early clinical trials have been suboptimal partially due to the immunosuppressive tumor *** this study,we used nanoparticles of folate(FA)-modified chitosan,a non-viral vector capable of targeting tumor cells with high expression of FA ***-chitosan nanoparticles were used as biological carriers for the expression plasmid of the mouse interferon-induced protein-10(mIP-10) gene,a potent chemoattractant for cytotoxic T *** combination of FA-chitosan/mIP-10 and DC/tumor cell fusion vaccine against hepatocellular carcinoma(HCC) effectively inhibited the growth of implanted HCC tumors and prolonged the survival of *** combination therapy significantly reduced myeloid-derived suppressor cells(MDSC) in mouse spleen,local tumor,and bone marrow while increasing tumor-specific IFN-γ ***,the combination therapy significantly inhibited tumor cell proliferation while promoting their *** together,our data illustrate that the mIP-10 enhances the anti-tumor effect of DC/tumor cell fusion vaccine by alleviating the immunosuppressive tumor environment.

关键词： DC/tumor fusion cell vaccines interferon-induced protein-10 folic acid chitosan

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

粗糙非饱和多孔介质有效热导率的分形分析

引用

工程热物理学报 2023年第8期44卷 2224-2232页

作者：邵义豪段楒量郭秀娅王会利王世芳郑仟武汉纺织大学数理科学学院武汉纺织大学应用数学与交叉科学研究中心武汉430200 合肥工业大学经济学院合肥230009 湖北第二师范学院物理与机电工程学院武汉430205

热导率在许多传热工程中发挥着重要作用,传统研究往往忽略了多孔介质孔隙表面的粗糙性,因此本文基于热电相似理论,考虑多孔介质内部孔隙微观结构的统计自相似特征,建立了具有粗糙表面的非饱和多孔介质中热导率的分形模型,详细分析了多... 详细信息

热导率在许多传热工程中发挥着重要作用,传统研究往往忽略了多孔介质孔隙表面的粗糙性,因此本文基于热电相似理论,考虑多孔介质内部孔隙微观结构的统计自相似特征,建立了具有粗糙表面的非饱和多孔介质中热导率的分形模型,详细分析了多孔介质的结构参数,比如孔隙率、粗糙度以及液相饱和度等对有效热导率的影响机理。理论研究结果与实验数据吻合较好,验证了本模型的正确性。此外,研究发现有效热导率随着液相饱和度的增加而增加,随着相对粗糙度的增加而降低。本模型没有经验参数,每个参数都有明确的物理意义。本文所提出的热导率模型可推广用于预测分形多孔介质的热输运性质。

关键词：分形多孔介质粗糙度热导率

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Generation of Dual functional Nanobody-Nanoluciferase Fusion and its potential in Bioluminescence Enzyme Immunoassay for trace Glypican-3 in Serum

Generation of Dual functional Nanobody-Nanoluciferase Fusion...

引用

第十四届全国免疫学学术大会

作者： siliang duan Sheng Yu Zhenfeng Li Jingzhang Li Shimei Zhao Hongjing Liu Shanguang Wu Xiongjie Bi Dongyang Li Jiexian Dong Bruce D. Hammock Medical College Guangxi University of Science and Technology The Second Clinical Medical College of Guangxi University of Science and Technology Department of Entomology and Nematology and UCD Comprehensive Cancer Center University of California Liuzhou People's Hospital The First Affiliated Hospital of Guangxi University of Science and Technology

Glypican-3(GPC3) is a serological biomarker for the diagnosis of Hepatocellular carcinoma(HCC), but it is a challenging task to develop a bioassay for determination of the trace GPC3 in serum. In this study, Bioluminescense immunoassay based on bifunctional nanobody-nanoluciferase fusion was developed with the ultra-sensitive feature to achieve this goal. First, nanobodies special against GPC3 binder as biological recognition element were generated by immunization and phage display technology. Second, The best clone GPN2 was fused with nanoluciferase as a dual-functional immunoreagent to establish an ultra-sensitive bioluminescence enzyme immunoassay(BLEIA), which is 30 and 5 times more sensitive than the traditional colorimetric assay and fluorescent assay, respectively. The cross-reactivity analysis of BLEIA showed that there was no cross-reactivity with HCC related tumor markers AFP, CEA, CA19-9 and GPC1/GPC2. The limit of detection(LOD) of developed BLEIA was 1.5 ng/mL, which assured its application in the diagnosis of GPC3 in 94 serum samples. This study indicates that BLEIA based on nanobody-nanoluciferase fusion could be used as a useful tool for the diagnosis of HCC patients.

关键词： Anti-GPC3 binder Nanobody/nanoluciferase fusion Bioluminescence enzyme immunoassay Diagnosis for HCC

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Folate-Modified Chitosan Nanoparticles Coating the Human IP-10 Gene Promote pMAGE-A1278-286 Specific CTL Responses to Hepatocellular Carcinoma in Mice

Folate-Modified Chitosan Nanoparticles Coating the Human IP-...

引用

第十六届中国科协年会——分13感染、免疫和疫苗论坛

作者： Khoune Sodaly Mong Nuo ZHOU Jian HE siliang duan Sufang ZHOU Chunhui LAI Xia YU Yi PENG Xiaoxun XIE Guorong LUO Yuan XIE Yongxiang ZHAO Xiaoling LU National Center for International Research of Biological Targeting Diagnosis and Therapy Guangxi Medical University Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research Guangxi Medical University Department of Oral and Maxillofacial Surgery the Affiliated Hospital of Stomatology Guangxi Medical University The Department of Immunology Guangxi Medical University

Adoptive therapy is a promising approach for the treatment of *** adoptive therapy,antigen-presenting cells(APCs)loaded with tumor specific antigens stimulate the proliferation of peripheral blood mononuclear cells(PBMCs)to produce tumor antigen-specific CTLs that are transferred back to patients to kill cancer ***,tumor antigen-specific CTLs are difficult to get to tumor tissues,due to immune suppression in ***-inducible protein-10(IP-10),a powerful chemokine,attracts CTLs to tumor tissues and improves their anti-tumor *** this study,we developed folate-modified chitosan nanoparticles coating the human IP-10 gene(FA-CS-hIP-10),which bound to the folate receptors on hepatoma cells and promoted the expression of IP-10 to improve the activity of pMAGE-A1278-286 specific *** combination of FA-CS-hIP-10 and pMAGEA1278-286 specific CD8+CTLs increased the secretion of IFN-γ,inhibited tumor growth and extended the survival of nude mice with subcutaneously transplanted human hepatocellular *** results demonstrated that the mechanism of this novel therapy approach was involved in the inhibition of angiogenesis,inhibition of proliferation,and promotion of apoptosis of tumor *** study provided a potentially novel approach for the treatment of human hepatocellular carcinoma by improving the activity of tumor antigen-specific CTLs.

关键词： cytotoxic T lymphocytes,chitosan,interferon-inducible protein-10,nanoparticles,folate receptor

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Folate-modified Chitosan Nanoparticles Containing the IP-10 Gene Enhance Melanoma-specific Cytotoxic CD8+CD28+ T Lymphocyte Responses

Folate-modified Chitosan Nanoparticles Containing the IP-10 ...

引用

第十二届全国免疫学学术大会

作者： He Jian duan siliang Yu Xia Qian Zhiyong Zhou Sufang Zhang Zhiyong Huang Xianing Huang Yong Su Jing Lai Chunhui Meng Jinyu Zhou Nuo Lu Xiaoling Zhao Yongxiang National Center for International Research of Biological Targeting Diagnosis and Therapy Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy ResearchCollaborative Innovation Center for Targeting Tumor Diagnosis and TherapyGuangxi Medical University State Key Laboratory for Biotherapy and Cancer Center West China HospitalSichuan University Department of Surgery Robert-Wood-Johnson Medical School University HospitalRutgers University Biomedical Polymers Laboratory Soochow University The Department of Immunology Guangxi Medical University

Background:Adoptive immunotherapy with cytotoxic T lymphocytes(CTLs) has great potential for the treatment of some malignant ***,augmenting the responses of tumor-specific CTLs is significant for the adoptive immunotherapy of *** study aimed to investigate the anti-tumor response of a combination therapy employing folate-modified chitosan nanoparticles containing IP-10(interferon-γ-inducible protein-10) plus melanoma TRP2-specific CD8+CD28+ T ***:We prepared folate-modified chitosan nanoparticles containing the mouse IP-10 gene(FA-CS-mIP-10),and induced melanoma TRP2-specific CD8+CD28+ T cells by co-culturing them with artificial antigen-presenting cells.B16-bearing mice were treated with FA-CS-mIP-10,melanoma TRP2-specific CD8+CD28+ T cells,a combination of both,and the saline *** volumes and the survival time of mice were *** proportion of myeloid-derived suppressor cells(MDSCs) infiltrating the tumor microenvironment and regulatory T cells(Tregs) in the spleen was analyzed by flow *** also detected the proliferation and angiogenesis of tumors by immunohistochemistry and apoptosis by ***:The combination therapy inhibited the progression of melanoma in *** with other treatments,it more efficiently inhibited tumor growth and increased the survival time of *** treatment with combination therapy,the proportion of MDSCs and Tregs decreased,while the percentage of CXCR3+CD8+ T cells ***,combination therapy inhibited proliferation and promoted apoptosis of tumor cells and significantly inhibited tumor angiogenesis in ***:We describe a novel strategy for improving the anti-tumor response of CD8+CD28+ CTLs by combining them with FA-CS-mIP-10 nanoparticles.

关键词： cytotoxic T lymphocytes melanoma folate chitosan interferon-γ-inducible protein-10

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

A new PD-1-specific nanobody improves T-cell-based tumor immunotherapy upon the induction of dendritic cell vaccine

A new PD-1-specific nanobody improves T-cell-based tumor imm...

引用

第十四届全国免疫学学术大会

作者： Xiaomei Yang wei shi Shenxia Xie Dani Zhong Xuandong Lin Ziqiang Ding siliang duan Fengzhen Mo Aiqun Liu Shihua Yin Xiaobing Jiang Zhi Ping(Gordon) Xu Xiaoling Lu Guangxi Medical University 广西医科大学附属肿瘤医院广西医科大学附属口腔医院华中科技大学同济医学院附属协和医院澳大利亚昆士兰大学生物工程与纳米技术研究所

Immune checkpoints impair the antitumor efficiency of T-cells in adoptive cell therapies(ACT),where PD-1/PD-L1 interaction play a key role in the negative effect of T-cell *** great success of PD-1 blockade,anti-PD-1 monoclonal antibody still demonstrates shortages,while nanobody(Nb) may fill the *** we report a strategy by using a new PD-1-blocking Nb(PD-1 Nb20) in combination of tumor-specific dendritic cell(DC)/tumor-fusion cell(FC) vaccine,to improve the activation,proliferation,cytokine secretion,and human tumor cell cytotoxicity of human CD8+*** PD-1 N b20 was found to effectively enhance the cytotoxicity of CD8+T-cells to kill human non-small cell lung cancer(NSCLC) HCC827 cells,hepatocellular carcinoma(HCC) HepG2 cells,and tongue squamous cell carcinoma(TSCC) Tca8113 cells in vitro,in combination of the DC/tumor-FCs generated by corresponding tumor cells,***,the PD-1 Nb20 and tumor-specific DC/tumor-FCs pre-treated CD8+T-cells significantly inhibited the growth of NSCLC-,HCC-or TSCC-derived xenograft tumors and prolonged survival of the tumor-bearing mice,through promoting T-cell infiltration to kill tumor cells and inhibiting tumor *** data demonstrate that this PD-1 Nb20 augments antitumor activity of T-cell upon the specific induction of DC/tumor-FC vaccine,and may provide an alternative and promising ACT strategy for cancer patients who are T-cell-dysfunctional or not sensitive to anti-PD-1 therapy.

关键词： Nanobody PD-1 blockade cytotoxic T lymphocytes dendritic cell/tumor fused cell vaccine tumorimmunotherapy

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Folate-Modified Chitosan Nanoparticles Coated Interferon-Inducible Protein-10 Gene Enhance Cytotoxic T Lymphocytes' Responses to Hepatocellular Carcinoma

Folate-Modified Chitosan Nanoparticles Coated Interferon-Ind...

引用

第十二届全国免疫学学术大会

作者： duan siliang Song Mongkhoune He Jian Zhou Nuo Zhou Sufang Zhao Jing Fang Yuan Peng Yi Huang Xianing Luo Guorong Lai Chunhui Yu Xia Zhang Zhiyong Xie Yuan Zhao Yongxiang Lu Xiaoling National Center for International Research of Biological Targeting Diagnosis and Therapy Guangxi Medical University Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research Guangxi Medical University Department of Oral and Maxillofacial Surgery the Affiliated Hospital of StomatologyGuangxi Medical University Department of Surgery Robert-Wood-Johnson Medical School University HospitalRutgers University The Department of Immunology Guangxi Medical University

Adoptive therapy using tumor antigen-specific cytotoxic T lymphocytes(CTLs) is a promising approach for treatment of human *** to immune suppression in cancer patients,it is difficult for tumor antigen-specific CTLs to arrive at tumor ***-inducible protein-10(IP-10) is a powerful chemokine that effectively attracts CTLs to tumor tissues and improves their anti-tumor *** over expression of IP-10 in tumor tissues can efficiently promote efficacy of adoptive ***-modified chitosan nanoparticles coating the human IP-10 gene(FA-CS-hIP-10) were therefore developed in this *** FA-CS-hIP-10 nanoparticles were specifically bound to folate receptors on hepatoma cells and promoted the expression of IP-10,to improve the activity of pMAGE-A1278-286 specific *** of the FA-CS-hIP-10 and pMAGE-A1278-286 specific CD8+ CTLs efficiently increased secretion of IFN-,inhibited tumor growth and extended survival of nude mice with subcutaneously transplanted human hepatocellular *** results demonstrated that the mechanism behind this novel therapeutic approach involved inhibition of angiogenesis and proliferation,and also promoted apoptosis of tumor *** study provides a potentially novel approach for treatment of human hepatocellular carcinoma by improving the activity of tumor antigen-specific CTLs.

关键词： Cytotoxic T Lymphocytes Chitosan Interferon-Inducible Protein-10 Nanoparticles Folate Receptor

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Folate-Modified Chitosan Nanoparticles Coated InterferonInducible Protein-10 Gene Enhance Cytotoxic T Lymphocytes' Responses to Hepatocellular Carcinoma

Folate-Modified Chitosan Nanoparticles Coated InterferonIndu...

引用

第十一届全国免疫学学术大会

作者： duan siliang Song Mongkhoune He Jian Zhou Nuo Zhou Sufang Zhao Jing Fang Yuan Peng Yi Huang Xianing Luo Guorong Lai Chunhui Yu Xia Zhang Zhiyong Xie Yuan Zhao Yongxiang Lu Xiaoling National Center for International Research of Biological Targeting Diagnosis and Therapy Guangxi Medical University Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research Guangxi Medical University Department of Oral and Maxillofacial Surgery the Affiliated Hospital of Stomatology Guangxi Medical University Department of Surgery Robert-Wood-Johnson Medical School University Hospital Rutgers UniversityThe State University of New Jersey The Department of Immunology Guangxi Medical University

Purpose:To improve the efficacy of adoptive immunotherapy,we aimed to develop a novel therapy approach to increase the concentration of tumor antigen-specific CTLs in tumor tissues and improve their anti-tumor ***:Chitosan nanoparticles were prepared using the ion template method,then folate and carbodiimide were then combined in a beaker and mixed in the dark using a magnetic stirrer for 1 *** diluted h IP-10 plasmid

关键词： Cytotoxic T Lymphocytes Chitosan Interferon-Inducible Protein-10 Nanoparticles Folate Receptor

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：