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Genotype Screening of Recipient Resources with High Regeneration and Transformation Efficiency in Chrysanthemum

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Phyton-International Journal of Experimental Botany 2022年第4期91卷 869-888页

作者： Yajun Li Yumeng Cui Bingjie Wang Yue Li Mengmeng Zhang silan dai He Huang Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular BreedingNational Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological EnvironmentKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of EducationMinistrySchool of Landscape ArchitectureBeijing Forestry UniversityBeijing100083China Beijing Botanical Garden Beijing100093China

Genetic transformation is one of the key steps in the molecular breeding of chrysanthemum,which relies on an optimal regeneration and transformation ***,the regeneration system of different chrysanthemum cultivars varies,and the regeneration time of most cultivars is *** screen cultivars with highly efficient regeneration,leaves and shoot tip thin cell layers(tTCL)from eight chrysanthemum cultivars with different flower colors and flower types were cultured on Murashige and Skoog media(MS)supplemented with 1.0-5.0 mg L^(−1)6-benzylaminopurine(6-BA)and 0.1-1.0 mg L^(−1)α-naphthaleneacetic(NAA).The results showed that the most efficient regeneration media were MS+6-BA 1.0 mg L^(−1)+NAA 0.5 mg L^(−1)for leaf explants and MS+6-BA 5.0 mg L^(−1)+NAA 0.1 mg L^(−1)for tTCL ***,another 13 chrysanthemum cultivars were screened by using the media,and finally,three cultivars with high regeneration efficiency were obtained from 21 *** these,C1 had the highest regeneration efficiency:the regeneration rate of leaf explants reached 80.0%after 42 days of culture,and the regeneration rate of tTCL explants reached 100%after 31 days of ***,we also established the transformation system for C1 as follows:preculturing for one day,infecting with Agrobacterium suspension(OD_(600)=0.6)for 10 min,and cultivating in the regeneration medium with 350 mg L^(−1)carbenicillin and 10 mg L^(−1)kanamycin,thus ultimately achieving a transformation rate of 4.0%.In this study,a new chrysanthemum cultivar with an efficient regeneration and transformation system was screened,which is beneficial to enrich the flower color of chrysanthemum transgenic plant recipients and to the functional research of flower color or type-related genes.

关键词： Chrysanthemum tissue culture regeneration system genetic transformation cultivars screening

Flavonoid extracts from chrysanthemum with appropriate anthocyanins turn blue when exposed to iron ions

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Horticultural Plant Journal 2024年第3期10卷 837-852页

作者： Yanfei Li Jiaying Wang Chenfei Lu Zhongman Wang Chengyan Deng Kang Gao Jingjing Li Zhijun Fang Hao Liu Yan Hong silan dai Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding National Engineering Research Center for FloricultureBeijingLaboratory of Urban and Rural Ecological EnvironmentKey Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of EducationSchool of Landscape ArchitectureBeijing Forestry UniversityBeijing 100083China Beijing Greenland Maintenance and Management Service Center Beijing 102211China Beijing North Asia Landscape Co. Beijing 102211China

Although some species that accumulate only cyanidin(Cy)in nature can produce blue flowers through iron ions,there has been no evidence of blue chrysanthemums being generated in this *** study revealed that flavonoid extracts from the ray florets of the chrysanthemum cultivar‘Wandai Fengguang’turned blue when exposed to Fe^(3+).Samples that could turn blue were labeled as CB(Cy-determined blue flowers),while samples that did not turn blue were labeled as CN(Cy-determined non-blue flowers).After a series of experiments,a stable screening system was established using flavonoid extracts containing NaAc buffer at pH 5.5 and a total anthocyanin concentration(TAC)of 30 μmol·L^(-1),and the addition of Fe^(3+)from 0 to 0.25 μmol·L^(-1)allowed for the selection of five CB samples from 39 chrysanthemum *** five CB samples exhibited flower color phenotypes that belonged to Cluster-I with redness(a*)values ranging from 29.03 to 45.99,yellowness(b*)values from-11.31 to 3.77,and brightness(L*)values from 29.07 to ***,the ratio of TAC to total luteolin concentration(TLC)was found to be a critical factor for distinguishing between CB and CN *** realize the desired blue hue in the flavonoid extracts with the participation of Fe^(3+),a TAC to TLC ratio of 2.25 and above is ***,the protoplasts and ray florets of CB samples that turned blue with the involvement of Fe^(2+)showed great potential for cultivating blue chrysanthemums through ferric-anthocyanin ***,this study reveals that blue flowers can be cultivated through the increase in the iron ion concentration,combined with the accumulation of Cy.

关键词： Chrysanthemum×morifolium Cyanidin Copigment Ferric-anthocyanin chelate Blue flower

High-density genetic map construction and identification of loci controlling flower-type traits in Chrysanthemum(Chrysanthemum×morifolium Ramat.)

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Horticulture Research 2020年第1期7卷 1469-1481页

作者： Xuebin Song Yuhui Xu Kang Gao Guangxun Fan Fan Zhang Chengyan Deng silan dai He Huang Huaigen Xin Yingying Li Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular BreedingNational Engineering Research Center for FloricultureBeijing Laboratory of Urban and Rural Ecological EnvironmentKey Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of the Ministry of EducationBeijing Forestry UniversitySchool of Landscape ArchitectureBeijing Forestry University35 East Qinghua RoadBeijing 100083China College of Landscape Architecture and Forestry Qingdao Agricultural UniversityQingdao 266109 ShandongChina Biomarker Technologies Co. LTDBeijing 101300China LC Science Co. LTD.Hangzhou 310018China

Flower type is an important and extremely complicated trait of *** corolla tube merged degree(CTMD)and the relative number of ray florets(RNRF)are the two key factors affecting chrysanthemum flower ***,few reports have clarified the inheritance of these two complex traits,which limits directed breeding for flower-type *** this study,305 F1 hybrids were obtained from two parents with obvious differences in CTMD and RNRF *** specific-locus amplified fragment sequencing(SLAF-seq)technology,we constructed a high-density genetic linkage map with an average map distance of 0.76 *** major QTLs controlling CTMD and four major QTLs underlying RNRF were repeatedly detected in the 2 ***,the synteny between the genetic map and other Compositae species was investigated,and weak collinearity was *** QTL regions with a high degree of genomic collinearity,eight annotated genes were probed in the Helianthus annuus *** Lactuca sativa *** ***,20 and 11 unigenes were identified via BLAST searches between the SNP markers of the QTL regions and the *** and *** transcriptomes,*** results lay a foundation for molecular marker-assisted breeding and candidate gene exploration in chrysanthemum without a reference assembly.

关键词： breeding controlling traits

Application of Real-time Fluorescent Quantitative PCR in Studies on Plants

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Agricultural Biotechnology 2012年第1期1卷 1-7页

作者： Yueping MA silan dai Yanrong MA College of Sciences Northeastern University College of Landscape Architecture Beijing Forestry University College of Agriculture Ningxia University

Real-Lime fluorescent quantitative PCR is a method for quantitative analysis of gene expression developed in recent years, which has been widely used in various fields such as basic scientific research, clinical diagnosis, disease study, drug research and development since its appearance. It starts relatively late in study on plants, but has already been used for analysis of gene expression in plants and gene identification of exogenous genes. The principles or advantages and dis- advantages of real-time fluorescent quantitative PCR, or its potential problems and condition optimizations in tests were introduced in this study, and then the appli- cation and prospect of real-time fluorescent quantitative PCR in study on plants were also been discussed.

关键词： Real-time fluorescent quantitative PCR (FQ-PCR) Plant C, ene expression

Effects of Different Treatment Methods on Virus Elimination of Chrysanthemum morifolium 'Shenma'

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Plant Diseases and Pests 2013年第6期4卷 5-9页

作者： Zhao Shuang Li Qing dai silan Beijing Forestry University

[Objective]The paper was to explore the virus elimination technique of Chrysanthemum morifolium′Shenma′.[Method]With *** ′Shenma′as the test materials,the virus elimination effects of shoot-tip treatment,shoot-tip with virazole treatment and shoot-tip with heat treatment were used to study the effect of virus elimination in the *** shoot-tips with the lengths of 0.2-0.3,0.4-0.5,0.6-0.8 and 0.9-1.0 mm were cultured by the shoot-tip treatment.A randomized complete blocks design was conducted to screen the best shoot-tip length and virazole concentration by shoot-tip with virazole *** test-tube seedling were cultured by the method of variable temperature at day and night and stepwise temperature *** test was used to identify the elimination efficiencies.[Result]The shoot-tip with the length of 0.4-0.5 mm was the best in shoot-tip treatment;the combination of shoot-tip length of 0.4-0.5mm and virazole mass concentration of 10 mg/L was the best in shoot-tip with virazole treatment;the shoot-tip with the length of 0.4-0.5 mm stripped after heat treatment for 45d was the best in shoot-tip with heat treatment.[Conclusion]The study provides an effective way for virus elimination in C. morifolium′Shenma′.

关键词： Chrysanthemum morifolium Cucumber mosaic virus Elimination

染色体原位杂交技术在植物亲缘关系研究中的应用

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北京林业大学学报 2000年第6期22卷 100-104页

作者：王文奎戴思兰北京林业大学园林学院北京100083

该文综述了近年来染色体原位杂交技术 (ISH)在植物亲缘关系研究中的应用 .文中介绍了 3种基于不同探针类型的ISH技术 ,以及该项技术在植物供体基因组的识别、不同供体基因组之间关系和物种形成过程中供体基因组的变化等方面的具体应用 ... 详细信息

该文综述了近年来染色体原位杂交技术 (ISH)在植物亲缘关系研究中的应用 .文中介绍了 3种基于不同探针类型的ISH技术 ,以及该项技术在植物供体基因组的识别、不同供体基因组之间关系和物种形成过程中供体基因组的变化等方面的具体应用 ,文章还指出了ISH在该领域的应用前景和它存在的局限性 .

关键词：染色体原位杂交植物亲缘关系供体基因组

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光受体介导信号转导调控植物开花研究进展

植物学报 2019年第1期54卷 9-22页

作者：马朝峰戴思兰北京林业大学园林学院国家花卉工程技术研究中心花卉种质创新与分子育种北京市重点实验室城乡生态环境北京实验室林木花卉遗传育种教育部重点实验室北京100083

光照是影响植物生长发育的重要环境因子,开花是高等植物生活史上最重要的事件。植物通过光受体感知外界环境中的光照变化,激活一系列信号转导过程从而适时开花。该文介绍了高等植物光受体的种类、结构特征和生理功能的研究进展,并系统... 详细信息

光照是影响植物生长发育的重要环境因子,开花是高等植物生活史上最重要的事件。植物通过光受体感知外界环境中的光照变化,激活一系列信号转导过程从而适时开花。该文介绍了高等植物光受体的种类、结构特征和生理功能的研究进展,并系统阐述了红光/远红光受体光敏色素、蓝光受体隐花色素以及FKF1/ZTL/LKP2等介导光信号调控植物开花的分子机制,包括光受体对CO转录及转录后水平调控和对FT转录水平的调控等。此外,还介绍了光受体整合光信号与温度和赤霉素等信号调控植物开花的研究进展,并展望了未来的研究方向。

关键词：光受体开花光敏色素隐花色素

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瓜叶菊花色性状形成机理的研究进展

植物遗传资源学报 2024年第5期25卷 695-703页

作者：崔宇萌黄河戴思兰北京林业大学园林学院/花卉种质创新与分子育种北京市重点实验室/国家花卉工程技术研究中心北京100083

瓜叶菊是拥有多种花色品种资源的观赏植物,同时具有蓝色、斑色等观赏植物中稀缺的花色表型,不同花色品种具有多种花青素代谢途径,解析其花色性状形成的分子机制能够为观赏植物的分子育种提供理论依据,其中鉴定的关键基因能够为蓝色花新... 详细信息

瓜叶菊是拥有多种花色品种资源的观赏植物,同时具有蓝色、斑色等观赏植物中稀缺的花色表型,不同花色品种具有多种花青素代谢途径,解析其花色性状形成的分子机制能够为观赏植物的分子育种提供理论依据,其中鉴定的关键基因能够为蓝色花新品种培育提供宝贵的基因资源。在对瓜叶菊多年研究的基础上,本文从瓜叶菊特殊的花青素结构、花青素生物合成调控途径及其花色研究的主要技术手段方面,综述了近20年来瓜叶菊花色研究的进展,具体内容包括:(1)瓜叶菊不同色系呈色的色素基础,尤其是蓝色花品种中特殊的多聚酰化色素结构;(2)多聚酰化修饰相关糖基化、酰基化修饰的瓜叶菊花青素代谢途径的基因,以及调控花色、花斑形成的MYB、MADS-box等转录因子的功能;(3)瓜叶菊中进行花色研究相关的高效遗传转化体系和病毒介导的基因沉默(VIGS,virus-induced gene silencing)体系,及其在花色研究中的研究进展。本文旨在为后续瓜叶菊及其他花卉花色研究和分子育种提供参考。

关键词：瓜叶菊花色花青素生物合成及调控

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环境因子调控植物花青素苷合成及呈色的机理

植物学报 2010年第3期45卷 307-317页

作者：胡可韩科厅戴思兰北京林业大学园林学院北京100083

花青素苷(anthocyanin)是决定被子植物花、果实和种皮等颜色的重要色素之一。花青素苷的合成与积累过程往往与植物发育过程密切相关,由内外因子共同控制。环境因子通过诱导植物体内花青素苷合成途径相关基因的表达来调控花青素苷的呈色... 详细信息

花青素苷(anthocyanin)是决定被子植物花、果实和种皮等颜色的重要色素之一。花青素苷的合成与积累过程往往与植物发育过程密切相关,由内外因子共同控制。环境因子通过诱导植物体内花青素苷合成途径相关基因的表达来调控花青素苷的呈色反应。该文追踪了国内外相关研究,认为光是影响花青素苷呈色的主要环境因子之一,光质和光强均能在一定程度上影响花青素苷的合成,其中光质起着更为关键的作用;低温能诱导花青素苷的积累,高温则会加速花青素苷的降解;不同的糖类物质均能影响花青素苷的合成,大部分结构基因和调节基因的表达均受糖调控。关于花发育与花青素苷呈色的关系、观赏植物花色对环境因子的响应以及花青素苷抵御逆境的机理尚待深入研究。因此,综合考察花发育与植物花青素苷合成及其呈色之间的关系,特别是光周期对花发育的影响导致花青素苷合成及呈色的机理是花色研究的一个重要课题。利用环境因子调控花色将会极大地提高花卉的观赏价值。

关键词：花青素苷呈色花青素苷合成环境因子基因调控