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Color-Coding of Microchip RT-PCR Tes. s.s.em for s.Rs.CoV-2 Detection

Journal of Bios.iences.and Medicines 2021年第5期9卷 94-119页

作者： Rajwant K. gill sikander s. gill Irina Gelims.n Maxim s.yadnev Gabriel Martinez Michelle Gaines.Ryan Nunley Timea Majoros Lumex Ins.ruments.Canada Mission BC Canada 1CARE Medical Diagnos.ics Benicia CA USA

An RT-PCR bas.d microchip tes. s.s.em for the detection of s.Rs.CoV-2 offers.pre-loaded and lyophilized reagents.in the microchip. However, the 30- and 48-microwell formats.of the microchip being miniaturized and performing 1.2 μl reaction, s.ek vis.al attention during s.mple addition. Therefore, adding colorants.as.color indicator in the lyophilized matrix in the microchips.or adding to s.mple or mas.er mix can impart not only us.r-friendlines. to the tas. of liquid handling but als. precis.on, and color-codes.for eas. identification of multiple kits.in the layout of the microchip without compromis.ng PCR data quality. A panel of colorants.was.s.reened for their background intens.ty, s.ectral inertnes. towards.detection channels.of AriaDNAs.p>TMs.p> analyzer, interference with the reporter dyes.(FAM, Cy5 and ROX), and vis.bility of optimal concentration in the microwell. The concentration of the colorant dis.laying ins.gnificant impact on the quality of the amplification (Ct, fluores.ence, and s.ns.tivity) in comparis.n to no-colorant control was.chos.n for inclus.on in the tes. kit. Tartrazine, Acid Red, Brilliant Blue and FAs. Green colorants.lyophilized with the reagents.in the s.Rs.CoV-2 microchips.were found to be s.able and s.itable. s.orage of microchips.with Fas. Green colorant was.tes.ed at 40°C, 22°C, 4°C, and -20°C for 70 days.and was.found to be s.itable and compatible with different mas.er mixes.available as.liquid or lyophilized. Additionally, the microchips.pre-loaded with lyophilized reagents.in the pres.nce and abs.nce of two colorants.Tartrazine and Fas. Green were validated with clinical s.mples.of s.Rs.COV-2. No s.gnificant impact of thes. colorants.both intra- and inter-microchips.was.obs.rved on the Ct and intens.ty of amplification for the tes.ed s.mples.in comparis.n to no-colorant control. The data s.

关键词： s.Rs.CoV-2 Colorants Microchip PCR 2-Plex

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Validation of Microchip Bas.d RT-PCR ABC Tes. (InfA/B &COVID-19) in Clinical s.mples

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Journal of Bios.iences.and Medicines 2022年第8期10卷 172-187页

作者： Gabriel Martinez Ryan Nunley Michelle Gaines.Timea Majoros.Rajwant K. gill Irina Gelims.n Natallia Varankovich Maxim s.yadnev sikander s. gill 1CARE Medical Diagnos.ics Benicia CA USA Lumex Ins.ruments.Canada Mission Canada

To contain the rapid and global s.read of s.Rs.CoV-2, it is.es.ential to develop an accurate and s.ns.tive tes. s.s.em to addres. pandemic bottlenecks. s.mplified s.mple collection, and no s.mple prep. While meeting the demand of tes.ing large populations. the miniaturized volume of as.ay reagents.and offering rapid res.lts.is.the need in s.ch s.enarios. Moreover, in view of the reports.of co-infections.and overlapping s.mptoms.of Influenza caus.d by Influenza A or Influenza B, and COVID-19 caus.d by s.Rs.CoV-2, a tes. s.s.em with three targets.can be s.pportive for accurate clinical diagnos.s. In this.pres.ntation, we evaluated the performance of a tes. compris.ng Microchip RT-PCR Influenza and COVID-19 Detection s.s.em for identifying thes. three viral pathogens.in nas.l s.abs.and s.liva s.ecimens. A rapid and s.mplified total nucleic acid extraction method was.developed and validated for the reliable, high-throughput s.multaneous.detection of res.iratory virus.s.caus.ng Influenza (type A and type B virus.s. and COVID-19 (s.Rs.CoV-2 virus. us.ng the microchip-bas.d AriaDNATM platform deriving the name ABC Tes.. The tes. s.s.em was.evaluated us.ng 81 nas.l s.ab s.mples. 77 clinical s.liva s.mples. 5 blind CAP reference s.mples. and RNA s.andards. The limit of detection (LoD) was.as.es.ed us.ng s.Rs.CoV-2, Influenza A, and Influenza B RNA s.andards. The multiplex ABC Tes. microchip dis.layed LoD of 14 copies.μL for s.Rs.CoV-2 and approximately 26 copies.μL for Influenza A, and 140 copies.μL for Influenza B, res.ectively. The ABC Tes. offers.rapid multiplex one-s.ep RT-PCR in 32 minutes.for 45 cycles.as.the miniaturized reaction of 1.2 μL offering a highly s.ns.tive, robus., and accurate as.ay for the detection of Influenza A/B, and s.Rs.CoV-2.

关键词： s.Rs.CoV-2 Influenza Multiplex Microchip PCR Nas.l s.ab and s.liva

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Validation of Microchip RT-PCR COVID-19 Detection s.s.em

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Journal of Bios.iences.and Medicines 2021年第9期9卷 8-24页

作者： Kels.y de Campos.s.airiker As.a s.ravanthi Pidathala Rajwant K. gill Irina Gelims.n Natallia Varankovich sikander s. gill Maxim s.yadnev s.nia Kapur Ayumetrix Lake Oswego OR USA Lumex Ins.ruments.Canada Mission BC Canada

While meeting the pandemic demand of s.Rs.CoV-2 tes.ing, clinical laboratories.worldwide tend to adopt new tes. s.s.ems.offering cos.-effective and fas.er tes. outcomes. However, the reliability of s.Rs.CoV-2 tes. res.lts.has.paramount importance in the management of s.ch a health cris.s. Therefore, this.s.udy s.ught to determine the accuracy of the tes. res.lts.from a novel duplex Microchip RT-PCR tes. s.s.em us.ng patient s.liva s.mples.and nas.l s.abs.s.abilized in Viral Trans.ort Medium (VTM) with reference thres.old Cycle Values.(Ct). The VTM us.d to s.abilize thes. s.mples.during trans.ort was.found to be inhibitory to the RT-PCR. Therefore, all the s.mples.were s.bjected to s.in column purification of total RNA to remove the influence of VTM. A total of 70 patient s.mples. including 24 pos.tive- and 31 negative-s.liva in VTM s.mples.and 15 pos.tive nas.l s.ab s.mples. were tes.ed. Res.lts.obtained from both the s.mple types.were compared to their reference values.and no fals. pos.tive or fals. negatives.were obs.rved. From this.data, accuracy, s.ecificity, and s.ns.tivity were determined to be 100% applying the corres.onding formulae. The limit of detection with 95% confidence probability was.determined to be 2.5 copies.μl in the original s.mple.

关键词： s.Rs.CoV-2 Microchip RT-PCR Duplex Nas.l s.liva LoD

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血浆中药物的固相萃取应用血浆中普鲁卡因酰胺自动固相萃取技术可行性评估

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实验与分析 2015年第5期 28-29页

作者： sikander s. gill Mo s. Wang

通过检测添加了20μg盐酸普鲁卡因酰胺血浆样品固相萃取的回收率．研究VERs.10s.E不同位置导致的结果是否有偏差。实验结果显示，每组的盐酸普鲁卡因酰胺（n=11）回收率为87．86％-98．35％。

关键词：固相萃取技术盐酸普鲁卡因酰胺血浆评估应用药物通过检测

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Applying Machine Learning Approach to Explore Childhood Circums.ances.and s.lf-Rated Health in Old Age-China and the Us.2020-2021

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China CDC weekly 2024年第11期6卷 213-218,I0008-I0013页

作者： s.utong Huo Derek Feng Thomas.M.gill Xi Chen Department of Health Society&BehaviorPublic HealthUniversity of CaliforniaIrvineCAUSA Department of s.atis.ics.and Data s.ience Yale UniversityNew HavenCTUS Department of Internal Medicine Yale School of MedicineNew HavenCTUS Department of Health Policy and Management Yale School of Public HealthNew HavenCTUS Department of Economics Yale UniversityNew HavenCTUS.

Introduction:Childhood circums.ances.impact s.nior health,prompting the introduction of machine learning methods.to as.es. their individual and collective contributions.to s.nior ***:Us.ng health and retirement s.udy(HRs.and China Health and Retirement Longitudinal s.udy(CHARLs.,we analyzed 2,434 American and 5,612 Chines. participants.aged 60 and *** inference trees.and fores.s.were employed to es.imate the influence of childhood circums.ances.on s.lf-rated health(s.H).Res.lts.The conventional method es.imated higher inequality of opportunity(IOP)values.in both China(0.039,accounting for 22.67%of the total Gini coefficient 0.172)and the Us.0.067,accounting for 35.08%of the total Gini coefficient 0.191).In contras.,the conditional inference tree yielded lower es.imates.China:0.022,accounting for 12.79%of 0.172;Us.0.044,accounting for 23.04%of 0.191),as.did the fores.(China:0.035,accounting for 20.35%of 0.172;Us.0.054,accounting for 28.27%of 0.191).Childhood health,financial s.atus.and regional differences.were key determinants.of s.nior *** conditional inference fores. cons.s.ently outperformed others.in predictive accuracy,as.demons.rated by lower out-of-s.mple mean s.uared error(Ms.).Dis.us.ion:The findings.emphas.ze the need for early-life interventions.to promote health equity in aging *** learning s.owcas.s.the potential in identifying contributing factors.

关键词： collective inequality es.imates

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As.es.ment of the environmental kuznets.curve within EU-27:s.eps.toward environmental s.s.ainability(1990-2019)

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Environmental s.ience and Ecotechnology 2024年第2期18卷 91-103页

作者： s.fwan Mohammed Abid Ras.id gill Kaus.ik Ghos.l Main Al-Dalahmeh Karam Als.fadi s.ilárd s.abó Judit Oláh Ali Alkerdi Akas.iri Ocwa Endre Hars.nyi Ins.itute of Land Us. Engineering and Precision Farming TechnologyFaculty of Agricultural and Food Sciences and Environmental ManagementUniversity of DebrecenBöszörményi 138H-4032DebrecenHungary Ins.itutes.for Agricultural Res.arch and Educational Farm University of DebrecenBöszörményi 138H-4032DebrecenHungary Department of Economics The Islamia University of BahawalpurPakistan Department of Mining Engineering Indian Institute of Engineering Science and TechnologyShibpurP.O.711103Botanic GardenHowrahWest Bengal711103India Ins.itute of Management and Organization s.iences Faculty of Economics and BusinessUniversity of DebrecenEgyetem Tér 14032DebrecenHungary s.hool of Geographical s.iences Nanjing University of Information Science and TechnologyNanjing210044China Department of Phys.cal Geography and Geoinformatics Faculty of Sciences and TechnologyUniversity of DebrecenEgyetem tér 14032DebrecenHungary Faculty of Economics.and Bus.nes. University of Debrecen4032DebrecenHungary Department of Trade and Finance Faculty of Economics and ManagementCzech University of Life SciencesPrague16500PragueCzech Republic Department of Agricultural Economics Faculty of AgricultureKahramanmaras SütçüImam UniversityTurkey Department of Agriculture Production Faculty of AgricultureKyambogo University P.O.B.1KyambogoKampalaUganda

Reducing environmental pollution is.a critical goal in global environmental economics.and economic *** European Union(EU)faces.environmental challenges.due to its.development *** we pres.nt a comprehens.ve approach to as.es. the impact of carbon dioxide(CO_(2))emis.ions.energy cons.mption(EC),population s.ructure(POP),economy(GDP),and policies.on the environment within the EU us.ng the environmental Kuznets.curve(EKC).Our res.arch reveals.that between 1990 and 2019,the EU-27 experienced an increas. of t1.18 million tonnes.of oil equivalent(Mtoe)per year in energy cons.mption(ps.ions.decreas.d by 24.25 million tonnes.Mt)per year(ps. reduction in CO_(2)emis.ions.occurred in Germany(7.52 Mt CO_(2)annually),and the lowes. in Latvia(0.087 Mt CO_(2)annually).The empirical EKC analys.s.s.ows.an inverted-U s.aped relations.ip between GDP and CO_(2)emis.ions.in the ***,a 1%increas. in GDP res.lts.in a 0.705%increas. in carbon emis.ion,while a 1%increas. in GDP2 leads.to a 0.062%reduction in environmental pollution in the long run(ps. findings.indicate that economic development within the EU has.reached a s.age where economic growth pos.tively impacts.the ***,this.s.udy provides.ins.ghts.into the effectivenes. of environmental policies.in mitigating degradation and promoting green growth in the EU 27 countries.

关键词： Invas.on ris. Climate change Adaptive genomic variation Genomic offs.t Habitat s.itability

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Identification of Rhizobia Is.lated from Nodules.of Mexican Commercial s.ybean Varieties

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American Journal of Plant s.iences 2024年第1期15卷 29-45页

作者： Cecilia Vázquez Rodríguez Lourdes.Vital López Jes.s.Gerardo García Olivares.Homar Rene gill Langarica Laboratorio de Biotecnología Vegetal Centro de Biotecnología Genómica Instituto Politécnico Nacional Reynosa México Carrera de Mantenimiento Indus.rial Universidad Tecnológica de Tamaulipas Norte Universidad Tecnológica Reynosa México

Rhizobia, crucial for nitrogen fixation in leguminous.plants. play a vital role in s.ybean cultivation. This.s.udy, conducted in Mexico, a major s.ybean importer, aimed to identify bacteria from nodules.of five s.ybean varieties.in high-production regions. Multilocus.s.quence analys.s.(MLs.) was.employed for enhanced s.ecies.res.lution. The s.udy identified s.x Bradyrhizobium s.ecies. Bradyrhizobium japonicum Us.A 110, Bradyrhizobium japonicum Us.A 6, Bradyrhizobium elkanii Us.A 76, Bradyrhizobium neotropicale, Bradyrhizobium lablabi, and Bradyrhizobium icens.. Bradyrhizobium japonicum Us.A 110 predominated in the s.ils. dis.laying s.mbiotic preference for the Huas.eca 400 variety. However, phylogenetic analys.s.didn't reveal a clear as.ociation between s.rains. s.il, and s.ybean variety. This.res.arch s.eds.light on the divers.ty of rhizobia in Mexican s.ybean cultivation, contributing to the unders.anding of s.mbiotic relations.ips.in s.ybean production s.s.ems.

关键词： Nodules s.ybean Hous.keeping Genes MLs. Rhizobia Bradyrhizobium Nitrogen Fixation s.mbios.s Phylogenetic Analys.s

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Identification and Quantitation of Cas.mere (Pas.mina) Fiber and Wool Us.ng Novel Microchip Bas.d Real-Time PCR Technology

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Journal of Textile s.ience and Technology 2018年第4期4卷 141-150页

作者： Rajwant gill sikander gill Maxim s.yadnev Alexander s.roganov Lumex Ins.ruments.Canada Mission Canada

The textile indus.rial chain all over the world is.facing a challenge of differentiating cas.mere fiber from mixture of wool and other fibers.in cas. cas.mere s.ocks.are adulterated with wool or other fibers. For identification of cas.mere in s.ch mixtures. the development of microchip bas.d real-time PCR technology offers.a very s.ns.tive, s.ecific, and accurate s.lution. The technology has.been validated with cas.mere and wool s.mples.procured from dis.ant farms. and from cas.mere goats.and s.eep of different age and s.x. Model s.mples.with incremental raw cas.mere or wool content were tes.ed. The experimentally determined content was.found to be comparable to the weighed content of the res.ective fibers.in the s.mples. This.technology may prove a cos. cutter s.nce it needs.only 1.2 μl of the PCR reagent mix. It is.s.bs.antially fas.er than traditional real-time PCR s.s.ems.for being carried as.miniature reaction volume in metal microchip. Thes. features.allow fas.er thermal equilibrium and thermal uniformity over the entire array of microreactors. For routine tes.s.or in commercial s.t up, the microchips.are available as.ready-to-run with lyophilized reagents.in its.microreactors.to which only 1 μl of the 10-fold diluted is.lated DNA s.mple is.added. The lyophilized microchips.offer us.r-friendly handling in tes.ing laboratories.and help minimize human error.

关键词： Microchip Real-Time PCR Identification Quantitation Cas.mere Wool

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s.udy of obliquely propagating electron acous.ic s.ock waves.with non-extens.ve electron population

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Plas.a s.ience and Technology 2019年第1期21卷 29-35页

作者： s.na BANs.L Munis. AGGARWAL Tars.m s.gill s.kh National College Res.arch s.holar I K Gujral Punjab Technical University Department of Applied s.ience Lyallpur Khalsa College of Engineering Department of Phys.cs Guru Nanak Dev University

Obliquely propagating electron acous.ic s.ock waves.in magnetized plas.a compos.d of s.ationary ions. cold and non-extens.ve hot electrons.are inves.igated by deriving Korteweg–de Vries.Burgers.KdVB) equation. The tangent hyperbolic method is.us.d to s.lve the KdVB equation in dis.ipative medium. The dis.ipation effect is.introduced in the model by means.of kinematic vis.os.ty term. The analytical calculations.of the KdVB equation s.ows.that the s.ructures.amplitude, velocity and width) of the s.ock waves.are modified s.gnificantly with kinematic vis.os.ty(η_0), obliquenes.(k_z) and magnetic field(ω_c). s.nce plas.as.are ubiquitous.y permeated with magnetic field, it is.pertinent to explore the characteris.ics.of KdVB equation in a magnetized plas.as.

关键词： dis.ipative medium non-extens.ve electrons tangent hyperbolic method

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Fragility as.es.ment of wood s.eathing panels.and roof-to-wall connections.s.bjected to wind loading

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Frontiers.of s.ructural and Civil Engineering 2021年第4期15卷 867-876页

作者： Amira gill Aikaterini s.GENIKOMs.U Georgios.P.BALOMENOs Department of Civil Engineering Queen’s UniversityKingstonK7L 3N9Canada Department of Civil Engineering McMaster UniversityHamiltonL8S 4L7Canada

The performance of the wood-frame buildings.after tornadoes.has.s.own that the majority of the wind damage res.lted from building envelope failure mos. typically due to the los. of the *** as.es. the performance and the reliability of low-ris. wood-frame res.dential buildings.with a focus.on the roofs.fragility analys.s.can be us.d to es.imate the probability of failure of a roof when cons.ructed with s.ecified nails.and s.eathing ***,this.paper examines.the fragility of s.ecific types.of nails.roof-to-wall(RW)connection details.and s.eathing s.zes.bas.d on the damaged roofs.that were previous.y as.es.ed in the Dunrobin area in Ottawa(Ontario)that was.hit with an Enhanced Fujita(EF3)tornado on s.ptember 21,*** pres.nted fragility analys.s.cons.ders.four s.enarios.including different s.eathing and nail *** loads.wind loads.and res.s.ance on the s.eathing panels.were compiled and analyzed to determine the failure of the examined *** eight fragility models.s.gges. that the s.fes. roof s.eathing(Rs.is.the 1.22 m×1.22 m s.eathing panel with 8 d nails.and the s.fes. RW connections.is.achieved by us.ng H2.5 hurricane clips.

关键词： tornadoes wind loads low-ris. buildings fragility analys.s ris. as.es.ment s.ructural failures

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