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Microbial reduction of graphene oxide and its application in microbial fuel cells and biophotovoltaics

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Frontiers of Materials Science 2023年第2期17卷 45-63页

作者： Jing-Ye Tee Fong-Lee Ng Fiona Seh-Lin Keng G.Gnana kumar siew-moi phang Institute of Ocean and Earth Sciences(IOES) Universiti Malaya50603 Kuala LumpurMalaysia Institute for Advanced Studies Universiti Malaya50603 Kuala LumpurMalaysia Department of Physical Chemistry School of ChemistryMadurai Kamaraj UniversityMadurai 625021Tamil NaduIndia Faculty of Applied Sciences UCSI University56000 Kuala LumpurMalaysia

Despite more than a decade of study,there are still significant obstacles to overcome before graphene can be successfully produced on a large scale for commercial *** oxidation of graphite to produce graphene oxide(GO),followed by a subsequent reduction process to synthesize reduced graphene oxide(rGO),is considered the most practical method for mass ***,which are abundant in nature and inexpensive,are one of the potential green reductants for rGO ***,there is no recent review discussing the reported microbial reduction of GO in *** address this,we present a comprehensive review on the reduction of GO by a range of microorganisms and compared their efficacies and reaction ***,presented were the mechanisms by which microorganisms reduce *** also reviewed the recent advancements in using microbially reduced GO as the anode and cathode material in the microbial fuel cell(MFC)and algal biophotovoltaics(BPV),as well as the challenges and future directions in microbial fuel cell research.

关键词： reduced graphene oxide microbial reduction microbial fuel cell algal biophotovoltaics green chemistry

Growth and photosynthesis of Chlorella strains from polar,temperate and tropical freshwater environments under temperature stress

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Journal of Oceanology and Limnology 2018年第4期36卷 1266-1279页

作者： Kok-Keong LEE Phaik-Eem LIM Sze-Wan POONG Chiew-Yen WONG siew-moi phang John BEARDALL Institute of Ocean and Earth Sciences University of Malaya Institute of Graduate Studies University of Malaya School of Health Sciences International Medical University National Antarctic Research Centre University of Malaya Institute of Biological Sciences University of Malaya School of Biological Sciences Monash University

Elevated temperatures as a consequence of global warming have significant impacts on the adaptation and survival of microalgae which are important primary producers in many ecosystems. The impact of temperature on the photosynthesis of microalgae is of great interest as the primary production of algal biomass is strongly dependent on the photosynthetic rates in a dynamic environment. Here, we examine the effects of elevated temperature on Chlorella strains originating from different latitudes, namely Antarctic, Arctic, temperate and tropical regions. Chlorophyll fluorescence was used to assess the photosynthetic responses of the microalgae. Rapid light curves(RLCs) and maximum quantum yield( F_v/F_m) were recorded. The results showed that Chlorella originating from different latitudes portrayed different growth trends and photosynthetic performance. The Chlorella genus is eurythermal, with a broad temperature tolerance range, but with strain-specific characteristics. However, there was a large overlap between the tolerance range of the four strains due to their "eurythermal adaptivity". Changes in the photosynthetic parameters indicated temperature stress. The ability of the four strains to reactivate photosynthesis after inhibition of photosynthesis under high temperatures was also studied. The Chlorella strains were shown to recover in terms of photosynthesis and growth(measured as Chl a) when they were returned to their ambient temperatures. Polar strains showed faster recovery in their optimal temperature compared to that under the ambient temperature from which they were isolated.

关键词： Antarctic Arctic Fv/Fm microalgae pigments recovery

Effects of elevated temperatures on growth and photosynthetic performance of polar Chlorella

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Advances in Polar Science 2020年第2期31卷 124-131页

作者： Syazana ANUWAR Ming-Li TEOH Wei-Hsum YAP Fong-Lee NG siew-moi phang School of Biosciences Taylor’s UniversityLakeside Campus47500 Subang JayaSelangor Darul EhsanMalaysia Institute of Ocean and Earth Sciences(IOES) University of Malaya50603 Kula LumpurMalaysia National Antarctic Research Centre Institute of Graduate Studies50603 Kula LumpurMalaysia Faculty of Applied Sciences UCSI University56000 Kuala LumpurMalaysia

Global warming has been the subject of concern in today’s world with elevating temperature causing the melting of polar ice and increasing sea *** aim of this study was to investigate the physiological and photosynthetic performance of two polar Chlorella,namely Chlorella UMACC 250 and Chlorella UMACC 234 to elevating temperatures as might be experienced under future warming *** cultures were exposed to three different temperatures of 4℃,8℃and 12℃.The growth and photosynthetic activity were determined every 2 d for a period of 10 *** the end of the experiment,the cultures were harvested and analysed for biochemical *** Chlorella strains were able to tolerate higher temperatures than their ambient *** final pigments content showed an increasing trend with increased temperatures for both *** photosynthetic activities were measured using pulse-amplitude modulation(PAM)*** photosynthetic parameters including maximum quantum efficiency(Fv/Fm),maximum relative electron transport rate(r ETRmax),light harvesting efficiency(a)and photoadaptive index(Ek)were derived from the rapid light curves(RLCs).Both Chlorella strains showed a slight decline in growth and photosynthetic activities at the initial part of the ***,they showed the ability to recuperate with Chlorella UMACC 250 recovers better compared to Chlorella UMACC *** Chlorella strains showed similar trend in their carbohydrate content at 12℃,while the protein content of Chlorella UMACC 234 decreased when exposed to increasing *** results indicated that polar Chlorella are able to survive at increased temperatures throughout the experiment.

关键词： polar Chlorella photosynthesis pulse-amplitude modulation(PAM)fluorometry

Sonication and grinding pre-treatments on Gelidium amansii seaweed for the extraction and characterization of Agarose

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Frontiers of Environmental Science & Engineering 2018年第4期12卷 17-23页

作者： Kit Wayne Chew Pau Loke Show Yee Jiun Yap Joon Ching Juan siew moi phang Tau Chuan Ling Jo-Shu Chang Department of Chemical and Environmental Engineering Faculty of Engineering University of Nottingham Malaysia Campus Jalan Broga 43500 Semenyih Selangor Darul Ehsan Malaysia Bioseparation Research Group Faculty of Engineering University of Nottingham Malaysia Campus Jalan Broga 43500 Semenyih Selangor Darul Ehsan Malaysia Department of Applied Mathematics Faculty of Engineering University of Nottingham Malaysia Campus Jalan Broga 43500 Semenyih Selangor Darul Ehsan Malaysia Nanotechnology & Catalysis Research Centre (NANOCAT) Institute of Postgraduate Studies University of Malaya 50603 Kuala Lumpur Malaysia Institute of Ocean and Earth Sciences University of Malaya 50603 Kuala Lumpur Malaysia Institute of Biological Sciences University of Malaya 50603 Kuala Lumpur Malaysia Department of Chemical Engineering National Cheng Kung University Tainan 701 Taiwan China

Various prctreatments methods including sonication and grinding were perfonned on red seaweed Gelidium amansii for the subsequent extraction ofagarose. The agarose products are usually extracted from agar powder products from seaweeds. In this study, the agarose was extracted using a direct polyethylene glycol （PEG） method without the need to first process the agar from seaweed. The agar extract was frozen then thawed and mixed directly with PEG solution to precipitate the agarose. The quality of agarose obtained was evaluated through physico-chemical properties analysis which includes spectral technique （FTIR）, melting and boiling point, gel strength and sulfate content. These properties were compared with a non-pretreated sample and it was found that the addition of pretreatment steps improved the quality of agarosc but gave a slightly lower yield. The gel strength of pretreated samples was much higher and the sulfate content was lower compared to non-pretreated samples. The best pretreatment method was sonication which gave gel strength of 742 gcm - and sulfate content of 0.63%. The extraction of agarose can be further improved with the use of different neutralizing agents. Pretreating the seaweed shows potential in improving the quality of agarosc from seaweed and can be applied for thture extraction of the agarose.

关键词： AgaroseGe lidium Pretreatment Seaweed Sonication

Inhibitory activities of microalgal extracts against Epstein-Barr virus DNA release from lymphoblastoid cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第5期12卷 335-345页

作者： Yih-Yih KOK Wan-Loy CHU siew-moi phang Shar Mariam MOHAMED Rakesh NAIDU Pey-Jiun LAI Shui-Nyuk LING Joon-Wah MAK Patricia Kim-Chooi LIM Pauline BALRAJ Alan Soo-Beng KHOO Division of Human Biology International Medical University Institute of Biological Sciences Faculty of Scienceand Institute of Ocean and Earth Sciences(IOES)University of Malaya Faculty of Medicine University of Malaya School of Medicine and Health Sciences Monash University Molecular Pathology Unit Institute for Medical ResearchJalan Pahang

This study aimed to assess the inhibitory activities of methanol extracts from the microalgae Ankistrodesmus convolutus,Synechococcus elongatus,and Spirulina platensis against Epstein-Barr virus(EBV) in three Burkitt's lymphoma(BL) cell lines,namely Akata,B95-8,and *** antiviral activity was assessed by quantifying the cell-free EBV DNA using real-time polymerase chain reaction(PCR) *** methanol extracts from Ankistrodesmus convolutus and Synechococcus elongatus displayed low cytotoxicity and potent effect in re-ducing cell-free EBV DNA(EC5028 000).After fractionation by column chromatography,the fraction from Synechococcus elongatus(SEF1) reduced the cell-free EBV DNA most effectively(EC50=2.9 μg/ml,therapeutic index>69).Upon further fractionation by high performance liquid chromatography(HPLC),the sub-fraction SEF1'a was most active in reducing the cell-free EBV DNA(EC50=1.38 μg/ml,therapeutic index>14.5).This study suggests that microalgae could be a potential source of antiviral compounds that can be used against EBV.

关键词： Microalgae Ankistrodesmus convolutus Synechococcus elongatus Spirulina platensis Lymphoblastoid cells Epstein-Barr virus(EBV)

Depth distribution and ecological preferences of periphytic algae in Kenyir Lake,the largest tropical reservoir of Malaysia

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Chinese Journal of Oceanology and Limnology 2010年第4期28卷 856-867页

作者： ROUF A.J.M.Abdur phang siew-moi AMBAK M.Azmi Institute of Oceanography Universiti Malaysia Terengganu Institute Ocean and Earth Sciences University of Malaya Department of Fisheries and Aquaculture Universiti Malaysia Terengganu

We studied the depth distribution of periphyton,growing on inundated dead trees in Kenyir Lake,Malaysia in June *** algal floral composition and structure manifested changes down the depth gradient in terms of species richness,abundance,diversity and cell *** regression analysis,all these algal attributes were negatively correlated with the depth gradients at P<*** terms of species richness,the bacillariophytes showed dominance over the cyanophytes and chlorophytes;whereas with respect to standing crop,the cyanophytes showed dominance over the bacillariophytes and *** chlorophyll a was higher at the mid and bottom-depths than the surface-depth in both the downstream and upstream sites,which showed that vertical productivity or biomass accumulation was greater in low light *** product-moment correlation analysis showed that conductivity,turbidity,dissolved oxygen,reactive phosphate and ammonium-nitrogen were highly correlated with the algal assemblage ***,photosynthetic active radiation(PAR) showed poor correlation with the community *** observations have cast some light on the autoecological characteristics,habitat preferences and environmental responses of tropical periphytic communities.

关键词： depth periphytic algae tropical large reservoir Kenyir Lake community structure biomass

Extraction of agar from Eucheuma cottonii and Gelidium amansii seaweeds with sonication pretreatment using autoclaving method

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Journal of Oceanology and Limnology 2019年第3期37卷 871-880页

作者： DIN Siti Sabariah CHEW Kit Wayne CHANG Yu-Kaung SHOW Pau Loke phang siew moi JUAN Joon Ching Department of Chemical and Environmental Engineering Faculty of EngineeringUniversity of Nottingham MalaysiaJalan BrogaSemenyih 43500Selangor Darul EhsanMalaysia Department of Chemical Engineering Ming Chi University of TechnologyNew TaipeiTaiwan 24301China Institute of Ocean and Earth Sciences University of MalayaKuala Lumpur 50603Malaysia Institute of Biological Sciences University of MalayaKuala Lumpur 50603Malaysia Nanotechnology & Catalysis Research Centre Institute of Postgraduate StudiesUniversity of MalayaKuala Lumpur 50603Malaysia

The effect of sonication pretreatment condition on Eucheuma cottonii and Gelidium amansii seaweed towards agar extraction wae *** parameters were changed during sonication to investigate the effects on agar yield and *** parameters include the time interval,concentration ratio,frequency,and *** highest amount of agar extracted from Eucheuma cottonii species could be obtained from the time interval of 30min,seaweed weight to solvent volume ratio of 1:20,the frequency of 35Hz,and the sonication power intensity of 30%.For Gelidium amansii species,the best agar yield also could be obtained from the time interval of 30 min,1:20 of seaweed weight to water volume ratio,the frequency of 35Hz,and power intensity of 30%.From the experiment,sonication pretreatment signifi cantly influenced the yield and properties of extracted *** sonication with autoclaved seaweed produced agar containing less sulfate content,which is an excellent chemical property for gel electrophoresis *** gelstrength of sonication with autoclaving for both seaweeds,Eucheuma and Gelidium species was the highest among those by sonication with direct heating,which proved that sonication pretreatment with autoclaving could enhance the physical properties of the agar.

关键词： Gelidium amansii Eucheuma collonii autoclaving sonication pretreatment water extraction

Effects of Boundary Conditions on the Design of Anti-Ram Bollards

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Transactions of Tianjin University 2008年第5期14卷 384-386页

作者： LIU Chunlin LI Guoqiang phang siew Kheong SUN Jianyun K＆C Protective Technologies Pte Ltd. 1 59361 Singapore School of Civil Engineering Tongji University Shanghai 200092 China

Anti-ram bollards used in perimeter protection are tested to meet performance requirements of established standards such as the US Department of State Specification SD-STD-02.01. Under these standards, tests are conducted in prescribed conditions that should be representative of the service installation. In actual project, conditions encountered on site may vary from the test environment and it would be expensive and time consuming to validate each deviation with a physical test. High-fidelity physics-based (HFPB) finite element modeling can provide precise simulations of the behavior of anti-ram bollards. This paper presents the use of HFPB finite element modeling, using LS-DYNA, in an actual project to evaluate the performance of an anti-ram bollard design subjected to various boundary conditions representing the physical conditions encountered on site. The study shows that boundary conditions can have a significant influence on the performance of the anti-ram bollards. This suggests that anti-ram bollards must be designed and engineered according to actual conditions that are found on site. It also shows that HFPB modeling can be an effective tool that supplements physical testing of anti-ram bollards.

关键词： anti-ram bollards perimeter protection high-fidelity physics-based modeling LS- DYNA boundary conditions

Pteropine Orthoreovirus(PRV7S)does not Establish Persistent Infection in NP460 Cells but with Inflammatory Response Triggered

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Biomedical and Environmental Sciences 2023年第9期36卷 886-891页

作者： LOH Mun Khin Alson siew Zhen Yun LEONG Pooi Pooi KOH Rhun Yian CHYE Soi moi WONG siew Tung VOON Kenny School of Postgraduate Studies International Medical University57000 Kuala LumpurMalaysia School of Pharmacy University of Nottingham Malaysia43500 SemenyihMalaysia Faculty of Medicine and Health Sciences Universiti of Tunku Abdul Rahman43000 KajangMalaysia School of Health Sciences International Medical University57000 Kuala Lumpur Malaysia School of MedicineInternational Medical University57000 Kuala LumpurMalaysia

Pteropine orthoreovirus(PRV)is a nonenveloped double-stranded RNA(dsRNA)virus of the genus Orthoreovirus under the family *** PRV genome is composed of 10 dsRNA segments,including three large(L)segments,three medium(M... 详细信息

关键词： PRV pine reovirus