检索结果-南通市图书馆

Analysis of 12 variants in the development of gastric and colorectal cancers

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2017年第48期23卷 8533-8543页

作者： Giovanna C Cavalcante Marcos AT Amador Andre M Ribeiro dos Santos Darlen C Carvalho Roberta B Andrade Esdras EB Pereira Marianne R Fernandes Danielle F Costa Ney PC Santos Paulo P Assumpcao Andrea Ribeiro dos Santos sidney Santos Laboratório de Genética Humana e Médica Universidade Federal do Pará Núcleo de Pesquisas em Oncologia Universidade Federal do Pará

AIM To evaluate the relation between 12 polymorphisms and the development of gastric cancer(GC) and colorectal cancer(CRC).METHODS In this study,we included 125 individuals with GC diagnosis,66 individuals with CRC diagnosis and 475 cancer-free individuals. All participants resided in the North region of Brazil and authorized the use of their samples. The 12 polymorphisms(in CASP8,CYP2 E1,CYP19 A1,IL1 A,IL4,MDM2,NFKB1,PAR1,TP53,TYMS,UGT1 A1 and XRCC1 genes) were genotyped in a single PCR for each individual,followed by fragment analysis. To avoid misinterpretation due to population substructure,we applied a previously developed set of 61 ancestryinformative markers that can also be genotyped by multiplex PCR. The statistical analyses were performed in Structure v.2.3.4,R environment and SPSS *** After statistical analyses with the control of confounding factors,such as genetic ancestry,three markers(rs79071878 in IL4,rs3730485 in MDM2 and rs28362491 in NFKB1) were positively associated with the development of GC. One of these markers(rs28362491) and the marker in the UGT1 A1 gene(rs8175347) were positively associated with the development of CRC. Therefore,we investigated whether the joint presence of the deleterious alleles of each marker could affect the development of cancer and we obtained positive results in all analyses. Carriers of the combination of alleles RP1 + DEL(rs79071878 and rs28361491,respectively) are at 10-times greater risk of developing GC than carriers of other combinations. Similarly,carriers of the combination of DEL + RARE(rs283628 and rs8175347) are at about 12-times greater risk of developing CRC than carriers of other *** These findings are important for the comprehension of gastric and CRC development,particularly in highly admixed populations,such as the Brazilian population.

关键词： Inflammatory processes Immune response Genomic and cellular stability Gastric cancer Colorectal cancer Amazon

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

索状炸药

火炸药

火炸药 1982年第1期 60-65页

作者： sidney A.Moses 高瑞

含有充填的燃烧剂或熔爆炸物的管或索可定义为索状炸药。外鞘材料与重量以及所含的炸药种类取决于使用要求。火药或推进剂在火炮或火箭中燃烧时产生热量和增压的气体。通常由金属粉末和氧化剂混合成的焰火剂,也包括在火药范畴之内。在... 详细信息

含有充填的燃烧剂或熔爆炸物的管或索可定义为索状炸药。外鞘材料与重量以及所含的炸药种类取决于使用要求。火药或推进剂在火炮或火箭中燃烧时产生热量和增压的气体。通常由金属粉末和氧化剂混合成的焰火剂,也包括在火药范畴之内。在炸弹或战斗部中炸药由猛炸药组成,它们在爆轰时产生破坏、冲击和破片。区分燃烧索状炸药与爆轰索状炸药的其它特性简要地概括于表1。在一定条件下,火药可以发生爆轰而炸药可以发生燃烧,它取决于某些因素如装填密度,限定的数量和引爆方法。

关键词：黑索今中速药芯 MDF 环三甲撑三硝胺硝胺类炸药索状炸药冲击感度 PETN 炸药爆轰导爆索爆轰传播延期索

Piwi like RNA-mediated gene silencing 1 gene as a possible major player in gastric cancer

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2018年第47期24卷 5338-5350页

作者： Taíssa Araújo Andre Khayat Luciana Quintana Danielle Calcagno Ronald Mourao Antonio Modesto Juliana Paiva Adhara Lima Fabiano Moreira Edivaldo Oliveira Michel Souza Moneeb Othman Thomas Liehr Eliana Abdelhay Renata Gomes sidney Santos Paulo Assumpcao Núcleo de Pesquisas em Oncologia Universidade Federal do ParáBelém 66073-000Brazil Laboratório de Cultura de Tecidos e Citogenética Instituto Evandro ChagasBelém 66087-082Brazil Institute of Human Genetics Universitatsklinikum JenaJena 07747Germany Laboratório de Célula Tronco Centro de Transplante de Medula OsseaInstituto Nacional de Cancer Jose Alencar Gomes da SilvaRio de Janeiro 20230-130Brazil

AIM To establish a permanent piwi like RNA-mediated genesilencing 1(PIWIL1) gene knockout in AGP01 gastric cancer cell line using CRISPR-Cas9 system and analyze phenotypic modifications as well as gene expression *** CRISPR-Cas9 system used was purchased from Dharmacon GE Life Sciences(Lafayette, CO, United States) and permanent knockout was performed according to manufacturer's recommendations. Woundhealing assay was performed to investigate the effect of PIWIL1 knockout on migration capability of cells and Boyden chamber invasion assay was performed to investigate the effect on invasion capability. For the gene expression analysis, a one-color microarray-based gene expression analysis kit(Agilent Technologies, Santa Clara, CA, United States) was used according to the protocol provided by the manufacturer. RESULTS PIWIL1 gene knockout caused a significant decrease in AGP01 migration capacity as well as a significant decrease in cell invasiveness. Moreover, functional analysis based on grouping of all differentially expressed m RNAs identified a total of 35 genes(5 up-regulated and 30 down-regulated) encoding proteins involved in cellular invasion and migration. According to current literature, 9 of these 35 genes(DOCK2, ZNF503, PDE4 D, ABL1, ABL2, LPAR1, SMAD2, WASF3 and DACH1) are possibly related to the mechanisms used by PIWIL1 to promote carcinogenic effects related to migration and invasion, since their functions are consistent with the changes observed(being up-or down-regulated after knockout). CONCLUSION Taken together, these data reinforce the idea that PIWIL1 plays a crucial role in the signaling pathway of gastric cancer, regulating several genes involved in migration and invasion processes; therefore, its use as a therapeutic target may generate promising results in the treatment of gastric cancer.

关键词： Gastric cancer Piwi like RNA-mediated gene silencing 1 CRISPR-Cas9 Migration Invasion

PRINCIPAL NORTH PACIFIC HEATING ANOMALY PATTERNS AND THEIR RELATIONS TO THE ATMOSPHERIC CIRCULATION

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Oceanology and Limnology 1989年第2期7卷 123-134页

作者：赵永平 G.A.McBean Institute of oceanology Academia Sinica Qingdao Canadian Climate Centre Institute of Ocean Sciences Sidney

The principal variability patterns (EOF) of the anomalies of total heat transfer from ocean to atmosphere computed from 30 years’ monthly averaged data over the North Pacific Ocean (20-60°N) showed variability was dominated by two patterns : a bipolar pattern and a dominantiy positive or negative pattern depending on the sign of the time series coefficients . The atmosphere contributes greatly to the marine heating anomalies in most of the North Pacific in all seasons. In winter, a positive feedback is formed between the Aleutian Low and the marine heating anomalies; in summer ,the marine heating anomalies are controlled by the heating on the Qinghai - Tibetan Plateau. Both patterns have a winter correlation with the Southern Oscillation Index.

关键词： winter Plateau heating latitude Tibetan dominated ocean averaged season bipolar

A SIMPLE,MANUAL SOLID-PHASE SYNTHESIS OF OLIGORIBONUCLEOTIDE

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1990年第2期1卷 179-182页

作者： Chun Guang WANG School of Pharmacy,Beijing Medical University Beijing 100083sidney M.HECHT Department of Chemistry,University of Virginia Charlottesville,VA22901,USA

A simple,manual solid-phase synthesis of RNA fragments using 2'-(p-methoxyben- zyl)nucleosides and phosphoramidite chemistry is described.

关键词： RNA A SIMPLE,MANUAL SOLID-PHASE SYNTHESIS OF OLIGORIBONUCLEOTIDE

Gross Chemical Profile and Calculation of Nitrogen-to-Protein Conversion Factors for Five Tropical Seaweeds

在线全文

学校读者我要写书评

暂无评论

American Journal of Plant Sciences 2011年第3期2卷 287-296页

作者： Graciela S.Diniz Elisabete Barbarino João Oiano-Neto sidney Pacheco Sergio O.Lourenco Departamento de Biologia Marinha Universidade Federal FluminenseNiteróiBrazil Laboratório de Cromatografia Líquida Embrapa Agroindústria de AlimentosRio de JaneiroBrazil Instituto Virtual Internacional de Mudanças Globais Universidade Federal do Rio de Janeiro—UFRJ/IVIGRio de JaneiroBrazil

Despite decades of research on marine algae, there are still significant gaps in basic knowledge about chemical composition of these organisms, especially in tropical environments. In this study, the amino acid composition and contents of total nitrogen, phosphorus, lipid, carbohydrate and protein were determined in Asparagopsis taxiformis, Centroceras clavulatum, Chaetomorpha aerea, Sargassum filipendula and Spyridia hypnoides. The seaweeds showed low lipid contents (lower than 5.5% d.w. in all species) and were rich in carbohydrates (more than 16% d.w. in all seaweeds). The percentage of nitrogen, phosphorus and protein varied widely among species, which red algae showed the highest con-centrations. The amino acid composition was similar among the seaweeds, which glutamic acid, aspartic acid and leu-cine as the most abundant. All species are poor in histidine. An average of 24.2% of the total nitrogen is non-proteinaceous. From data of total amino acid and total nitrogen, specific nitrogen-to-protein conversion factors were calculated for each species. The nitrogen-to-protein conversion factors calculated ranged from 4.51 to 5.21, with an overall average of 4.86. These findings show that the traditional conversion factor of 6.25 should be avoided for seaweeds, since it overestimates the actual protein content.

关键词： Nitrogen-to-Protein Conversion Factors Protein Amino Acid Seaweeds Element Composition Tropical Environment

Legal opinion letters formbook 2nd ed.

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Legal opinion letters formbook 2nd ed.

2003年

作者： A. sidney Holderness Jr. Brooke Wunnicke editors

来源：畅想之星图书光盘版（南... 评论

在线全文

畅想之星图书光盘版（南通市图书馆镜像）

学校读者我要写书评

暂无评论

博扬·斯莱特:清理海洋垃圾的少年

新东方英语（中学版） 2015年第2期 39-41页

作者： sidney Stevens 赵青奇

"这行不通。"博扬·斯莱特最初提出一种清理污染海洋的数百万吨塑料垃圾的方法时,屡屡听到这样的回答。换成其他任何人,绝大部分都会因为挫折和绝望知难而退。但是,20岁(编注:英文原文发表于2014年8月)的斯莱特却从未退缩。"人类的历史... 详细信息

"这行不通。"博扬·斯莱特最初提出一种清理污染海洋的数百万吨塑料垃圾的方法时,屡屡听到这样的回答。换成其他任何人,绝大部分都会因为挫折和绝望知难而退。但是,20岁(编注:英文原文发表于2014年8月)的斯莱特却从未退缩。"人类的历史基本上就是一部不可能最终成为可能的历史。"他说。如今,斯莱特及其"海洋清理"项目团队正在不断取得进展,证明那些质疑者错了。这对地球来说是个好消息。

关键词：斯莱特项目团队塑料垃圾英文原文质疑者废弃塑料 ocean 浮栅 decided Pursuit

Quantification of acylglycines in human urine by HPLC electrospray ionization-tandem mass spectrometry and the establishment of pediatric reference interval in local Chinese

在线全文

学校读者我要写书评

暂无评论

Quantification of acylglycines in human urine by HPLC electr...