检索结果-南通市图书馆

Advances in Microbiology 2014年第4期4卷 202-206页

作者： sideney becker.onofr. Dirlane Antoniazzi Departamento de Ciências Biológicas e Farmacêuticas Faculdade Educacional de Francisco Beltrao—FEFB Uniao de Ensino do Sudoeste do Paraná—UNISEP Francisco Beltrao Paraná Brasil

The apple is the second most important fruit in Brazil. However, apple cultivars are susceptible to several diseases that can cause losses after harvesting. Bitter rot is caused by the fungus Colletotrichum gloeosporioides and is one of the most damaging summer diseases. The goal of this work was to evaluate the behavior of this fungus in four apple cultivars grown in Brazil (Fuji, Gala, Golden and Green) under two treatments: direct inoculation and isolated fungus. The fungus was isolated by taking fragments from infected fruits, which were stored on Potato Dextrose Agar (PDA) in a laboratory. For the direct treatment, the fungus was removed from infected fruits and directly inoculated into healthy fruits. After inoculation, the fruits were kept at room temperature and the halos of degradation were evaluated every 48 hours. The results demonstrated that direct inoculation was more pathogenic, that the Gala cultivar was the most resistant to the pathogen, and that the Golden cultivar was the most susceptible.

关键词： Diseases Biotechnology Phytopathogens Fungi Pathology

Presence of Staphylococcus aureus in the Nasal Cavity of Children Attending a Public Daycare Center in Francisco Beltrao—Parana—Brazil

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Infectious Diseases 2013年第2期3卷 89-94页

作者： sideney becker.onofr. Géssica Araceli Costa Department of Biological Sciences and Pharmacy Faculty of Educational of Dois VizinhosUniao de Ensino do Sudoeste do ParanáDois VizinhosBrazil.

The objective of this study was to evaluate the presence of Staphylococcus aureus in the nasal cavity of children attending a daycare center in the town of Francisco Beltrao—PR, by comparing the frequency between age groups and genders. Antimicrobial susceptibility testing with different drugs was also carried out in the bacterial isolates. A prospective study involving 200 children aged 2 and 4 years, grouped by gender, was carried out. Samples were collected from the nasal vestibules of children, incubated and identified. Antimicrobial susceptibility testing was carried out in 30% of the S. aureus colonies. The results showed that 35% of children (10% female and 25% male) were colonized by S. aureus. The frequency of colonization in both male age groups was similar, with 201.93 ± 13.10 CFU/plate for 2-year-old and 266 ± 12.60 CFU/plate for 4-year-old. However, colonization averages for females were different, with 30.43 ± 1.17 CFU/plate for 2-year-old and 394.38 ± 10.70 CFU/plate for 4-year-old. In the antimicrobial susceptibility testing, some strains were resistant to oxacillin, erythromycin and tetracycline, in addition to showing intermediate susceptibility to cephalothin.

关键词： Nasal Cavity Frequency Susceptibility Antimicrobials

Action of Essential Oils Obtained from Baccharis coridifolia D.C.(Asteraceae-Astereae)on the Activity of Antibiotics

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Microbiology 2013年第2期3卷 166-170页

作者： sideney becker.onofr. Marilde Canton Paula Andressa Pires Department of Biological Sciences and Pharmacy FAED—Faculty of Educational of Dois Vizinhos—Uniao de Ensino do Sudoeste do Paraná—UNISEP—Bairro Nossa Senhora AparecidaDois VizinhosParanáBrazil

The aim of this study was to investigate the effects of Baccharis coridifolia essential oil on the Activity of Antibiotics. Assays were performed with ampicillin (10 μg), cephalothin (30 μg), chloramphenicol (30 μg), gentamicin (10 μg) and tetracycline (30 μg) alone and in combination with the essential oil (4% v/v) through the disk diffusion susceptibility test. The results showed the effects of essential oil on the activity of the antibiotics tested. Zones of inhibition of bacterial growth with different diameters were observed surrounding the antibiotic disks, whether or not they were impregnated with the essential oil. The occurrence of the synergistic or antagonistic effect was observed in both bacterial strains assessed—Staphylococcus aureus (ATCC-25923) and Escherichia coli (ATCC-25922). These results show that the use of products derived from plants can, in some cases, interfere with the effectiveness of antibiotics during clinical therapy.

关键词： Medicinal Plants Essential Oils Antibiotic Associated Use Interference

Cellulase Production by Endophytic Strains of Trichoderma reeseifrom Baccharis dracunculifoliaD. C. (Asteraceae)

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Microbiology 2014年第5期4卷 275-283页

作者： sideney becker.onofr. Taismara Bonfante Zípora Morgana Quinteiro dos Santos Marielly Coradin de Moura Aline Filakowski Cardoso Departamento de Ciências Biológicas e da Saúde Universidade Paranaense UNIPAR Unidade Universitária de Francisco Beltr&#227o Francisco Beltr&#227o Paraná Brasil Laboratório de Microbiologia Departamento de Ciências Biológicas da Uni&#227o de Ensino do Sudoeste do Paraná UNISEP Campus de Francisco Beltr&#227o Francisco Beltr&#227o Paraná Brasil

Cellulases are enzymes responsible for the degradation of cellulose, the major compound in plant cells. Cellulose is a polysaccharide composed of several glucose units linked together by chemical bonds. Cellulases, such as endoglucanases, beta-glucosidase and exoglucanases, break the chemical bonds between the glucose units. Fungi, including the endophytic species, can be great cellulase producers. This study aimed to evaluate cellulase production by four endophytic strains of Trichoderma reesei semi-solid media containing sugarcane bagasse, supplemented or not with salts. Two fermentations were carried out for 43 days. Samples were taken every seven days to obtain production peaks. The enzymes were characterized by their optimum pH and temperature of activity and stability upon incubation in the presence of ions, pH and temperature variations. The results showed that the endophytic strains FB1, FB2, FB3 and FB4 of Trichoderma reesei produce cellulases in a sugarcane bagasse medium, supplemented or not with salts, at pH 5.5 and 30°C. The supplemented medium proved to be more appropriate to induce cellulase production after 29 days of fermentation, with FB4 having the best yield: 16.32 ± 2.65 IU/gram of fermented substrate.

关键词： Substrate Fermentation Processes Enzymes Cellulases